Figure 1 | Scientific Reports

Figure 1

From: Mapping the virome in wild-caught Aedes aegypti from Cairns and Bangkok

Figure 1

Bioinformatics work-flow. The bioinformatics work-flow is shown with read numbers and where the final data is presented (blue). Raw sequence reads were quality filtered using Trimmomatic and paired end reads were merged using the software PEAR. Ae. aegypti reads were identified using STAR and BWA mem and used for variant analyses. Non-mosquito reads were subsequently aligned to a custom ribosomal database using BLAST. Reads not matching a ribosomal reference sequence were taxonomically assigned using DIAMOND and the lowest common ancestor (LCA) approach. Non-chordate sequences were analyzed using BLAST to identify blood meal species. Contigs assembled with Trinity from non-mosquito and non-ribosomal reads were taxonomically assigned using the best BLAST hit.