Deafness and loss of cochlear hair cells in the absence of thyroid hormone transporters Slc16a2 (Mct8) and Slc16a10 (Mct10)

Sharlin, David S.; Ng, Lily; Verrey, François; Visser, Theo J.; Liu, Ye; Olszewski, Rafal T.; Hoa, Michael; Heuer, Heike; Forrest, Douglas

doi:10.1038/s41598-018-22553-w

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Published: 13 March 2018

Deafness and loss of cochlear hair cells in the absence of thyroid hormone transporters Slc16a2 (Mct8) and Slc16a10 (Mct10)

David S. Sharlin^1,2^na1,
Lily Ng²^na1,
François Verrey ORCID: orcid.org/0000-0003-3250-9824³,
Theo J. Visser⁴,
Ye Liu²,
Rafal T. Olszewski⁵,
Michael Hoa⁵,
Heike Heuer⁶ &
…
Douglas Forrest²

Scientific Reports volume 8, Article number: 4403 (2018) Cite this article

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Abstract

Transmembrane proteins that mediate the cellular uptake or efflux of thyroid hormone potentially provide a key level of control over neurodevelopment. In humans, defects in one such protein, solute carrier SLC16A2 (MCT8) are associated with psychomotor retardation. Other proteins that transport the active form of thyroid hormone triiodothyronine (T3) or its precursor thyroxine (T4) have been identified in vitro but the wider significance of such transporters in vivo is unclear. The development of the auditory system requires thyroid hormone and the cochlea is a primary target tissue. We have proposed that the compartmental anatomy of the cochlea would necessitate transport mechanisms to convey blood-borne hormone to target tissues. We report hearing loss in mice with mutations in Slc16a2 and a related gene Slc16a10 (Mct10, Tat1). Deficiency of both transporters results in retarded development of the sensory epithelium similar to impairment caused by hypothyroidism, compounded with a progressive degeneration of cochlear hair cells and loss of endocochlear potential. Administration of T3 largely restores the development of the sensory epithelium and limited auditory function, indicating the T3-sensitivity of defects in the sensory epithelium. The results indicate a necessity for thyroid hormone transporters in cochlear development and function.

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Introduction

Neurodevelopment is known to require adequate thyroid hormone in the circulation but for many years, little attention was given to questions of access of the hormone to target tissues^1,2. The necessity for proteins that transport hormone across the plasma membrane of cells was indicated by finding mutations in solute carrier SLC16A2 (monocarboxylate transporter 8, MCT8) in Allan-Herndon-Dudley syndrome, an X-linked disorder of psychomotor and speech retardation^3,4. SLC16A2 has 12 transmembrane domains and transports the active form of thyroid hormone, T3, and its precursor T4⁵. In vitro studies have identified other proteins that transport T3 or T4 among other substrates, suggesting that the current picture of thyroid hormone transport is far from complete⁶. Moreover, it has been suggested that Slc16a2 cooperates with organic anion transporter Oatp1c1 (Slco1c1)⁷ or L-type amino acid transporter Lat2 (Slc7a8)⁸ in the mouse brain, raising the possibility that combinations of transporters extend control over additional developmental functions.

Auditory development is highly sensitive to thyroid hormone⁹. Hearing loss is associated with endemic iodine deficiency¹⁰, resistance to thyroid hormone¹¹ and congenital hypothyroidism^12,13. In rodents, the cochlear sensory epithelium, which contains the mechanosensory hair cells, is a major site of T3 action^14,15,16. The Thrb thyroid hormone receptor gene promotes developmental remodeling of the sensory epithelium prior to the onset of hearing^17,18,19. Thyroid hormone also influences hair cell survival, and cochlear functions including the endocochlear potential^19,20 as well as the maintenance of hearing²¹.

The compartmentalized anatomy of the cochlea led us to hypothesize that membrane transporters are necessary to convey blood-borne hormone to its target tissues²². Blood enters the cochlea through the spiral modiolar artery then branches through radiating arterioles to capillary networks in the lateral wall before draining through collecting venules and the spiral modiolar vein²³. These vessels bypass the sensory epithelium, implying a need for mechanisms that transport T3 and T4 internally. A need for transport is further implied by the requirement in auditory development for type 2 deiodinase, which amplifies levels of T3 by conversion from T4²². Type 2 deiodinase is expressed in the medial cochlea and lateral wall in proximity to blood vessels but distant from the sensory epithelium²⁴. We previously reported expression of Slc16a2 and a related thyroid hormone transporter Slc16a10 (Mct10)²⁵ in cochlear tissues²⁶. Here we report deafness and loss of hair cells in mice lacking Slc16a2 and Slc16a10. Treatment with T3 partly rescued phenotypes, supporting a critical role for transmembrane transport of T3 for the development and maintenance of the cochlea.

Results

Auditory deficits in mice lacking Slc16a2 and Slc16a10

To investigate the requirement for Slc16a2 and Slc16a10 for hearing, we analyzed the auditory-evoked brainstem response (ABR). In Slc16a2- or Slc16a10-deficient mice, auditory thresholds were similar to those in wt mice at 6–12 weeks of age (approximately young adult ages). However, in double knockout (dko) mice lacking both transporters, thresholds were markedly elevated compared to wt mice (Fig. 1A), thus unmasking a critical combined role for these transporters for auditory function. Responses in dko mice were defective for a click (broad band of frequencies) or pure tone stimuli at frequencies of 8, 16 and 32 kHz that span the sensitive range of hearing in mice. Defects persisted in older dko mice (6–9 months old), indicating a permanent loss of hearing. Severe impairment was also evident in weanling dko mice (3–4 weeks old), indicating an early-onset of hearing loss (not shown).

Analysis of ABR waveforms showed that compared to wt mice, the residual responses that could be detected in dko mice were much diminished in magnitude (Fig. 1B). When subjected to a click stimulus at equivalent high intensity (90 dB sound pressure level), the mean amplitude of the first peak was severely reduced by ~90% in dko compared to wt mice (Fig. 1C). In ~50% of dko mice, no specific waveform could be detected for a click stimulus. Amplitudes were also reduced in Slc16a2 and Slc16a10 single mutants, suggesting that although thresholds were in the normal range, the magnitude of the response was subtly compromised in the absence of either transporter alone (Fig. 1C).

In addition to defects in the ABR, an overall measure of auditory function, dko mice also displayed impairment of the distortion product otoacoustic emission (DPOAE) (Fig. 1D), a measure of the function of cochlear outer hair cells during the response to sound. Furthermore, the endocochlear potential (EP), the positive potential difference in the endolymph in the scala media that is considered necessary for auditory transduction, was substantially reduced in adult dko mice compared to wt mice (Fig. 1E). These results indicate that cochlear defects contribute to hearing loss in dko mice.

T4 and T3 levels in dko mice

We investigated levels of circulating thyroid hormone in dko mice as it is known that overt deficiency of thyroid hormone at early postnatal stages causes hearing loss in mice²⁷. At P6, during the period of cochlear remodeling before hearing normally begins, serum levels of T4 and T3 were not significantly different in dko compared to wt groups (Fig. 2). In wt mice, T4 and T3 levels rise to peak between P12 and P16, then decline slightly after weaning^24,28. In dko mice during this period, T4 was reduced, although only to a similar extent as in Slc16a2 mutant mice, which have minimal auditory defects. In adult 20-week old dko mice, T4 was normal and T3 was ~2-fold elevated. Similar trends for T4 and T3 have been reported for dko mice at 3 weeks of age, shortly after hearing normally begins²⁹.

Slc16a10 mutants displayed minimal changes in T4 and T3 compared to wt mice at any age examined. Slc16a2 mutants, as reported previously^29,30, displayed an approximately 50% decrease in T4 and an ~2-fold increase in T3 as adults and a similar trend at juvenile ages (P12 - P21). In summary, dko mice displayed levels of T4 and T3 that would normally be expected to suffice for the development of hearing. Moreover, changes in T4 and T3 in the dko, which have severe deafness, were more modest than in Slc16a2 single mutants, which have minimal auditory defects. Thus, systemic hormonal changes are unlikely to explain the deafness in dko mice.

Developmental and degenerative defects in the sensory epithelium

Histological examination of adult dko mice at 3 months of age revealed degeneration of the sensory epithelium with the most severe degeneration in upper (more apical) regions of the cochlear spiral (Fig. 3A). The dko mice displayed loss of outer hair cells, inner hair cells and support cells with a flattened residual epithelium. A more variable disorganization of the sensory epithelium was observed in lower (more basal) regions of the cochlea. The tectorial membrane, which extends over the inner sulcus to the hair cells and is critical for auditory transduction³¹, appeared moderately swollen, resembling a characteristic feature of hypothyroid mice^15,32. Slc16a2 or Slc16a10 single mutants displayed no overt defects in cochlear morphology (Fig. 3B), suggesting that either transporter alone can promote and maintain cochlear structure, consistent with the minimal auditory impairment observed in either single mutant. In the dko, the overall structure of the cochlear chambers (scala media, scala tympani, scala vestibuli) was intact (Fig. 3C).

At postnatal stages (P7) (Fig. 4A), the immature sensory epithelium of dko mice contained a normal complement of cell types including hair cells, indicating that the loss of cells observed at older ages arose from degeneration rather than developmental failure to form cell types. Figure 4B shows a reference diagram of cell types in the immature cochlea.

However, dko pups displayed retardation of cochlear remodeling (Fig. 4A), a defect typical of hypothyroid^15,20,32 or Thrb-deficient²¹ mice. At P7, the sensory epithelium in wt mice begins to acquire mature morphology: the cell mass of the greater epithelial ridge regresses, the inner sulcus opens beneath the tectorial membrane and the tunnel of Corti opens between the pillar cells. These events progress in a basal-to-apical direction and thus are more advanced in lower than upper regions. In dko pups at P7, the greater epithelial ridge had not regressed, the inner sulcus and tunnel of Corti had not opened and the tectorial membrane was malformed. Remodeling was retarded in both lower and upper cochlear regions in dko mice at P7.

Remodeling did eventually progress in dko mice but lagged persistently behind the developmental status in wt mice. At P15 in wt mice, soon after the onset of hearing, the greater epithelial ridge had largely regressed, the hair cells and supporting cells had an upright structure and the tunnel of Corti was open. However, in dko mice, the greater epithelial ridge had only partly regressed, hair cells and supporting cells were poorly elongated and the tunnel of Corti was only partly open. The retardation was less pronounced in lower regions of the cochlea consistent with development occurring eventually in a basal-to-apical direction as in wt mice.

By P21, degeneration of the sensory epithelium was evident in upper cochlear regions in dko mice (Fig. 4A). In severe examples, the tunnel of Corti had collapsed and only sparse cells remained in the epithelium. The greater epithelial ridge retained an excessive cell mass, indicating persistently retarded development. In lower cochlear regions, degeneration was initially less obvious and remodeling had progressed to some extent to give a more normal morphology. Variable degeneration was also evident in dko mice at P18 (Fig. 4C). In summary, juvenile dko mice displayed a complex phenotype involving both retardation and a variable degeneration evident during the third postnatal week.

Progression of degeneration

As an indicator of the long-term progression of degeneration of the sensory epithelium, hair cells were counted on histological sections up to 6 months of age (Fig. 4D). At P7 or P15, numbers of hair cells in the dko mice were not significantly abnormal in upper or lower cochlear regions. However, by P28-P30, in upper regions, numbers of outer and inner hair cells in dko mice were reduced by ~75% and at 6 months, by ~90% compared to wt mice. Loss of hair cells in middle and lower cochlear regions was less severe up to P28-P30 but was notable by 6 months at which stage ≥45% of outer and inner hair cells were missing.

Morphology of the stria vascularis and spiral ganglion

Given the defective endocochlear potential, we investigated the stria vascularis, which has a role in maintaining homeostasis in the endolymph in the scala media³³. No major histological abnormality was evident in the stria vascularis or adjacent fibrocyte layer of the spiral ligament (Fig. 5A). The area and thickness of the stria vascularis measured on cochlear sections were in the normal range (Fig. 5C). Analysis for markers of marginal (Kcnq1, Slc12a2), intermediate (Kcnj10) and basal (Cldn11) cell types of the stria vascularis indicated the presence of all three cell layers. However, the intensity of the Kcnj10 signal was increased in the dko mice compared to wt in middle and upper regions of the cochlea (Fig. 5B,C) suggesting an abnormality in the status of the stria vascularis. Reissner’s membrane was not obviously distorted, suggesting that fluid pressure in the scala media or scala vestibuli was not grossly abnormal (Fig. 3C).

The dko mice also began to lose spiral ganglion cells concomitantly with loss of hair cells after ~2 weeks of age (Fig. 5D,E). By P28, in upper regions of the cochlea in dko mice, almost 30% of spiral ganglion cells were lost and by 6 months, ~60% were lost compared to wt mice. By 6 months, a 36% loss of spiral ganglion cells was also evident in mid-lower regions of the cochlea.

Rescue of cochlear remodeling by exogenous T3

To test the hypothesis that the cochlear phenotypes in dko mice result from insufficient T3 reaching target tissues, the T3-dependence of phenotypes was determined by administration of exogenous T3. The experiments were based on the assumption that high doses of T3 would overcome impaired uptake by tissues through utilization of other routes, potentially involving lower affinity types of thyroid hormone transporters.

To investigate the developmental retardation of the cochlea, dko pups were injected with T3 or saline vehicle for three consecutive days at P0-P2, then examined histologically at P5 (Fig. 6A). In wt pups, as expected³⁴, T3 accelerated the regression of the greater epithelial ridge and the opening of the inner sulcus and tunnel of Corti (Fig. 6B). In dko pups, T3 similarly stimulated regression of the greater epithelial ridge, partial opening of the inner sulcus and tunnel of Corti and thinning of the tectorial membrane. T3 recovered a similar degree of opening of the inner sulcus in dko pups as in wt pups (Fig. 6C). T3 at higher doses also stimulated opening of the tunnel of Corti in dko pups to a similar extent as in wt pups.

Preservation of the sensory epithelium by exogenous T3

The degeneration of the sensory epithelium in dko mice at more mature ages was preventable by administration of T3 over a later time course (Fig. 7A). T3 was given by continuous provision in the drinking water from P7 until analysis at P28. Previous studies demonstrated that exogenous T3 administered in the first few postnatal days produces long term deafness whereas T3 given at P7 or later does not cause hearing loss³⁴. Thus, treatment starting at P7 was not expected to cause additional auditory damage.

In wt mice with or without T3 treatment, no loss of hair cells nor malformation of the sensory epithelium was observed at P28 (Fig. 7B). In untreated dko mice, as expected, the epithelium was flattened and lacked hair cells, especially in upper cochlear regions. However, in dko mice, T3 treatment remarkably recovered a more normal cochlear morphology. Inner and outer hair cells, pillar cells and other supporting cell types were restored and the sensory epithelium regained an upright structure with an open tunnel of Corti (Fig. 7B,C). A small cluster of cells remained near the base of the inner hair cells suggesting that T3 treatment started at P7 was unable to correct fully the early regression of the greater epithelial ridge in dko mice. Counts on histological sections showed that T3 prevented loss of inner and outer hair cells (Fig. 7E). These results were confirmed in an independent experiment using an alternative route of administration of T3 by daily injection over the same time-course (not shown, see Methods). T3 given from P7 to P28 also recovered spiral ganglion cell numbers in the dko mice (Fig. 7D,E).

Auditory function after treatment with exogenous T3

To determine if T3 treatment also recovered auditory function in dko mice, the ABR was analyzed at P28 following administration of T3 in drinking water from P7 to P28 (Fig. 8A,B).

In wt mice, as expected for treatment over this period³⁴, T3 produced little or no change in ABR thresholds compared to untreated mice (Fig. 8A). In dko mice, T3 stimulated a limited recovery of the ABR. Thresholds were slightly lower in T3-treated than untreated dko groups across a range of frequencies. Figure 8C shows representative waveforms for a click stimulus in which the untreated dko lacked discernible peaks, even with high intensity stimuli (up to 100 dB SPL) whereas the T3-treated dko displayed distinct peaks. Similar improvements in the definition and amplitude of the waveform in dko mice were observed for pure tone stimuli. The mean amplitude of response was unchanged by T3 in wt groups but was enhanced in dko groups, as shown in Fig. 8D for a click and a representative pure tone stimulus (16 kHz) when delivered at equivalent high intensity (90 dB SPL).

Mice treated with T3 in the same way (in drinking water from P7) were also tested for recovery of the endocochlear potential at P28 - P30 (Fig. 8E). Compared to wt mice, untreated dko mice at ~P30 displayed a reduced endocochlear potential resembling the defect in adult dko mice (see Fig. 1E). T3 did not rescue the endocochlear potential in dko mice at P28 - P30. Thus, the endocochlear potential in dko mice was not restored by treatment with T3 under conditions that rescued hair cell survival and partly rescued the ABR.

Discussion

This study demonstrates a key role for Slc16a2 (Mct8) and Slc16a10 (Mct10) transporters in the development of hearing and maintenance of cochlear structure. The findings suggest that apart from the systemic provision of thyroid hormone, cellular uptake or efflux of the hormone by transmembrane transporters is required to promote cochlear development and hearing.

Studies of Allan-Herndon-Dudley syndrome and of mice with Slc16a2 mutations have established a requirement for Slc16a2 for T3 action in the brain and pituitary-thyroid axis but less is known about Slc16a10. In mice, Slc16a10 facilitates transport of aromatic amino acids in liver and kidney³⁵, consistent with initial descriptions of this protein as a T-type amino acid transporter³⁶. However, Slc16a2 and Slc16a10 share a related transmembrane structure and ~53% amino acid identity, which led to the finding that Slc16a10 also transports T3 and less efficiently T4, across the plasma membrane of cells in vitro^25,37,38. Deletion of Slc16a10 in Slc16a2-deficient mice modifies the levels of T4 and T3 in serum, kidney and brain, suggesting that both transporters cooperate in a complex manner to determine the thyroid hormone status of certain tissues²⁹.

Our study reveals a new role for Slc16a10 in the auditory system and suggests that this role, at least in part, involves transport of T3. First, the retarded cochlear morphology in dko mice resembles that of hypothyroid¹⁵ or Thrb-deficient²¹ mice. Secondly, cochlear morphology in the dko is substantially rescued by administration of T3. Thus, although Slc16a10 can transport aromatic amino acids and T3 in vitro, the particular substrate utilized in vivo may depend on tissue and developmental context. Conceivably, the substrate specificity is governed in part by the co-expression of other types of transporters, such as Slc16a2 in cochlear cell types.

Overt hearing loss arises only in the absence of both transporters indicating that Slc16a10 and Slc16a2 compensate functionally for each other in the auditory system. The simplest explanation may be direct substitution since in situ hybridization²⁶ revealed partly overlapping expression of both transporters in the inner sulcus, sensory epithelia, Claudius cells and tympanic border cells. The developmental expression profiles are complex with Slc16a10 following Slc16a2. The induction of Slc16a10 at ~P5 may provide a temporal cue for the remodeling of the cochlea. Type 2 deiodinase, a T3-amplifying enzyme that is required for cochlear development²², is also induced in the cochlea at this time²⁴ suggesting that coordinated mechanisms drive the T3-dependent remodeling that precedes the onset of hearing. However, the level of Slc16a10 is low and detailed analysis at cellular resolution awaits improved methods to detect the protein.

The solute carrier Slc16A family, includes 14 members and was originally named a monocarboxylate transporter (Mct) family as several members transport monocarboxylate substrates such as lactate and pyruvate³⁹. Among this family, Slc16a10 and Slc16a2 constitute a distinct subgroup based on protein sequence and substrate preference for iodothyronines (T4 and T3)²⁵. Our finding of cooperation for these transporters in the auditory system together with previous evidence in the endocrine system²⁹ raise the possibility that Slc16a2 and Slc16a10 together may be found to mediate additional functions for thyroid hormone in vivo.

In the cochlea, we proposed that different transporters demarcate a cellular route that transfers thyroid hormone from the bloodstream to tissues within the cochlea²⁶. The phenotype of the dko mice supports a role for Slc16a2 and Slc16a10 in transport of T3 in sensory or adjacent tissues, in which expression of the transporters overlaps partly with that of the Thrb receptor gene²¹. The initial steps for uptake of T4 and T3 from the circulation may involve other types of transporters including the L-type amino acid transporter Lat1 in endothelial cells and the organic anion transporting polypeptide Oatp1c1 (Slco1c1) in tissues adjacent to capillaries in the spiral ligament and modiolus²⁶.

The expression of Slc16a2 and Slc16a10 in Claudius cells and other support cells leads to speculation that these cells might form a conduit for relaying hormone internally from the lateral wall or medial cochlea to the sensory epithelium. Slc16a2 and Slc16a10 mediate both uptake and efflux of T3²⁵, which may allow cell-to-cell relay of hormone. Interestingly, other intercellular transport functions have been attributed to Claudius, Deiters, Hensen and root cells and another more medial cellular network in the spiral limbus and inner sulcus. These cells form epithelial cell gap junctional networks⁴⁰ that may transfer potassium ions away from the hair cells to the lateral wall of the cochlea⁴¹. The transport of ions, metabolites and nutrients by specialized epithelial cells maintains homeostasis in the cellular and fluid compartments of the cochlea³³. Our findings suggest that cell-mediated transport of hormones may be similarly critical to cochlear function.

Deafness in the dko is severe and is comparable to that caused by deletion of all T3 receptors¹⁹ suggesting that Slc16a2 and Slc16a10 together transport most of the thyroid hormone required by the immature cochlea under normal conditions. However, the rescue experiments imply that other lower affinity thyroid hormone transporters are utilized when T3 is given in excess. In common with proposals for the brain^2,42, the cochlea may express different classes of transporters: high specificity types such as Slc16a2 and Slc16a10 and other types, perhaps with a wider spectrum of substrates that may not normally transport thyroid hormone.

Allan-Herndon-Dudley syndrome is associated with impaired speech but there have been few remarks on hearing, perhaps reflecting a lack of obvious defects or lack of detailed analysis in some cases. One report has mentioned hearing impairment³ but others have not⁴³. It is conceivable that as in mice, compensation by SLC16A10 masks auditory defects in patients with SLC16A2 (MCT8) mutations. Nonetheless, subtle defects in auditory function or auditory processing might contribute to defective speech in this syndrome. Mutations in human SLC16A10 have not been reported.

Our study indicates a remarkable ability of T3 to rescue cochlear structure and hair cell survival in dko mice. Degenerative forms of hearing loss are common in human populations and may result from many environmental or genetic factors⁴⁴. Regarding possible therapeutic approaches to deafness, much effort has focused on hair cells because these cells cannot regenerate naturally in mammals⁴⁵. Our results suggest that T3 may be worth testing in attempts to preserve hair cells in experimental models of deafness. The pattern of hair cell loss in the dko mice with an initial bias to upper cochlear regions is unusual and has not been observed in hypothyroid or Thrb-deficient mice. On the contrary, Thrb-deficient mice display most hair cell loss in the lower cochlea²¹, suggesting that unknown factors contribute to the regional patterns of degeneration. In dko mice, hair cell loss might be a secondary defect as auditory function was impaired at all frequencies tested and did not obviously correlate with tonotopical domains along the length of the cochlea.

The loss of endocochlear potential would impair hearing in dko mice and may reflect defects in the tissues of the lateral wall³³. Interestingly, dko mice display elevated expression of Kcnj10 in the intermediate cells of the stria vascularis. In hypothyroid mice, the expression of Kcnj10 is reduced in the stria vascularis²⁰ and the cerebral cortex⁴⁶, consistent with this gene being T3-responsive. The increased expression of Kcnj10 in the stria vascularis in dko mice may result from the elevated T3 levels in the dko. Kcnj10 is required for an endocochlear potential in mice⁴⁷ and mutations in KCNJ10 occur in a syndromic form of human deafness⁴⁸. T3 treatment recovered cochlear morphology and a limited ABR but not endocochlear potential. The persistent defect in endocochlear potential may explain why T3 did not rescue auditory function to a greater extent. The results also support a view that the survival of the sensory epithelium is not strictly dependent on the endocochlear potential⁴⁹.

It is possible that in dko mice, early events that promote the endocochlear potential are disrupted before the beginning of T3 treatment at P7, or that no alternative transporters for T3 are present in tissues that generate the endocochlear potential. A role for T3 in determining the endocochlear potential is consistent with a partial loss of endocochlear potential reported in mice lacking thyroid hormone receptors¹⁹ and in hypothyroid dwarf mice²⁰. T3 may act directly in lateral wall tissues that contribute to the endocochlear potential as the Thrb gene is expressed in root cells and fibrocytes of the spiral ligament²¹, as well as in the sensory epithelium and spiral ganglion. It is not excluded that the loss of endocochlear potential reflects impaired transport of substrates other than T3.

Finally, we note that the complex auditory phenotype of dko mice may involve additional defects not in the sensory epithelium or lateral wall, as T3 also influences the spiral ganglion^26,50 and brainstem auditory nuclei⁵¹.

Methods

Mouse Strains

The null allele of Slc16a2 (Mct8) was previously reported⁵². The Slc16a10 (Mct10) null allele carries a premature stop at codon 88 (Y88*), which truncates most of the protein including 11 of 12 transmembrane domains³⁵. Double mutant (dko) mice were originally on a C57BL/6 (Charles River) background²⁹ and at NIH were further backcrossed for up to 2 generation onto a C57BL6/J (Jackson Lab) background. Slc16a2-deficient groups were generated by crossing Slc16a2+/− females with y/− males. Slc16a10-deficient groups were generated by crossing Slc16a10+/− males and females. Groups of dko mice were generated by crossing Slc16a10+/−; Slc16a2+/− females with either Slc16a10+/−; Slc16a2y/− males or Slc16a10−/−; S lc16a2y/− males. Controls were wt littermates from these crosses. For some T3 treatment experiments, wt progeny were intercrossed to generate additional wt mice on the same genetic background. Genotypes were determined by PCR as described^35,52. Most analyses were performed on mixed groups of males and females in approximately equal numbers. Male-only groups were used for hormone measurements. Animal studies were conducted under approved protocols at NIDDK and NIDCD at NIH and Minnesota State University Mankato. All methods were performed in accordance with relevant guidelines and regulations.

Thyroid hormone treatment

T3 (Sigma-Aldrich) was provided to pups in the drinking water or by daily sub-cutaneous injection in the nape of the neck, as described^22,53 for the time courses noted in Figs 6, 7 and 8. Previous studies reported that thyroid hormone is transferred through the milk of nursing dams at neonatal stages⁵⁴. Short term postnatal treatment involved injections on P0, P1 and P2 with 0.2 μg or 1.5 μg T3 in saline or with saline alone in a 10 μl volume. Longer term treatment was given by injections from P7 to P28-P30 with 0.5 μg T3 in saline or saline alone in a 10 μl volume (4–11 mice), or by T3 added in drinking water at 0.5 μg/mL from P7 to P28 (5–6 mice).

Auditory Function Tests

The auditory-evoked brain stem response (ABR) was analyzed using a SmartEP system (Intelligent Hearing Systems, Miami, FL) as described⁵³. Mice were anesthetized with tribromoethanol (avertin) (0.25 mg/g body weight). Active, reference and ground electrode needles were placed subcutaneously at the vertex, ventrolateral to the left ear, and ventrolateral to the right ear, respectively. Groups (mixed males and females) at 6–12 weeks of age, contained 14 wt, 7 Slc16a10 ko, 8 Slc16a2 ko and 8 dko mice. Groups at 3–4 weeks of age contained 7 wt and 9 dko. Groups at 6–9 months of age contained 10 wt and 10 dko. For dko mice with no detectable response, a threshold was assigned at 100 dB SPL, the maximum stimulus level possible. The Distortion Product Otoacoustic Emission was analyzed using a Smart DPOAE system (Intelligent Hearing Systems) on 8 week old mice (groups of 7) under avertin anesthesia, as described⁵³.

Methods for endocochlear potential measurement have been previously described by Wangemann and colleagues^55,56. Briefly, groups (at ~P90 and ~P28-P30) were anesthetized with avertin (0.35 mg/g body weight). Endocochlear potential measurements were made using glass microelectrodes inserted into the round window and through the basilar membrane of the first turn of the cochlea. Induction of anoxia, allowing measurement in the anoxic-state, was accomplished by intramuscular injection of succinylcholine chloride (0.1 µg/g body weight) after establishment of deep anesthesia followed by additional injection of avertin. Measurement in the anoxic-state provides an indicator of the lowest endocochlear potential and hair cell function: in the presence of functional hair cells, the anoxic-state endocochlear potential is negative, whereas the endocochlear potential is zero if the hair cells are not functional. In all adult dko mice at ~P90, bony overgrowth of the bulla was noted and clear fluid was observed to arise from the region of the round window in a continuous fashion prior to insertion of the electrode for recording the endocochlear potential. Data were recorded digitally (Digidata 1440 A and AxoScope 10; Axon Instruments) and analyzed using Clampfit10.

Measurement of Thyroid Hormones

Serum T4 and T3 levels were determined from trunk blood samples collected from male mice by radioimmunoassay (RIA) as described⁵⁷. Hormone values represent individual mice. Groups contained 3–12 male mice per genotype at P12, P14–16, P21, and 20 weeks of age. At P6, groups of 3–5 mice per genotype were measured as pools consisting of 3 mice. Hormone values at P21 have been previously published and are included in Fig. 2 for completeness²⁹.

Histology

Cochleae were dissected from the temporal bone, fixed overnight in phosphate buffered saline (PBS) containing 3% glutaraldehyde/2% paraformaldehyde, decalcified in 0.1 M ethylenediaminetetraacetic acid (EDTA) in PBS, dehydrated through a graded series of ethanol concentrations (50, 70, 80, 95, 100%) then embedded in glycol methacrylate plastic (Polysciences, Warrington, PA) as described⁵³. Four μm thick sections in the vertical, mid-modiolar plane were stained with hematoxylin Gill #3 (Sigma), air-dried, then coverslipped in Permount (Sigma-Aldrich). At least one cochlea/mouse for ≥3 mice was analyzed at each age.

Immunofluorescence

For immunohistochemistry of the lateral wall, adult inner ears were fixed, then decalcified in 150 mM EDTA for 5–7 days, transferred to 30% sucrose, embedded then frozen in SCEM tissue embedding medium (Section-Lab Co, Ltd.; Hiroshima, Japan). Adhesive film (Section-Lab Co, Ltd.; Hiroshima, Japan) was fastened to the cut surface of the sample in order to support the section and cut slowly with a blade to obtain 6 µm thickness sections. The adhesive film with section attached was submerged in 100% ethanol for 60 seconds, then transferred to distilled water. The adhesive film consists of a thin plastic film and an adhesive and prevents specimen shrinkage and detachment. This methodology allows for high quality anatomic preservation of the specimen and allows sectioning at reduced thickness (to 0.5 µm). Mid-modiolar sections were obtained for samples in which an endocochlear potential recording had been performed.

For analysis of stria vascularis markers, mid-modiolar sections were washed in PBS then permeabilized and blocked for 1 hour at room temperature in PBS with 0.2% Triton X-100 (PBS-T) with 10% fetal bovine serum. Samples were incubated with primary antibodies in PBS-T with 10% fetal bovine serum, followed by three rinses in PBS-T, then incubated with AlexaFluor-conjugated secondary antibodies (1:250, Life Technologies) in PBS-T for 1 hour at room temperature. Where indicated, 4,6-diamidino-2-phenylindole (DAPI, 1:10,000, Life Technologies) was included with secondary antibodies to detect nuclei. Sections were rinsed in PBS three times and mounted in SlowFade (Invitrogen), then imaged using a Zeiss LSM 710 confocal microscope. Sections were mounted with SCMM mounting medium (Section-Lab Co, Ltd.; Hiroshima, Japan). Primary antibodies included rabbit anti-Kcnj10 (Alomone Labs, Cat# APC-035, polyclonal, dilution 1:200), rabbit anti-Cldn11 (Life Technologies, Cat# 364500, polyclonal, dilution 1:200), goat anti-Slc12a2 (Santa Cruz Biotech, Cat# sc-21545, polyclonal, dilution 1:200), goat anti-Kcnq1 (Santa Cruz Biotech, Cat# sc-10646, polyclonal, dilution 1:200), Phalloidin AlexaFluor 647 (Invitrogen, Cat# A22287, dilution 1:250).

Cell Counts and Histological Measurements

Images were acquired for upper, middle and lower turns of the cochlea on 4 μm thick methacrylate sections in the mid-modiolar, vertical plane. The most extreme apical and basal regions were avoided. For developmental studies, hair cell and spiral ganglion cells were counted on 4 cochlear sections per mouse (groups of 4–11) at P7, P15, P28–30 and ~P180. Spiral ganglion cell counts were normalized relative to the surrounding area of Rosenthal’s Canal using the ImageJ program. For T3 treatment experiments, spiral ganglion cells were counted on 1–3 cochlear sections per mouse (groups 3–4 mice). Areas of the inner sulcus and tunnel of Corti were measured on middle turns of the cochlea on 3 sections per cochlea (groups of 3–7 mice) using ImageJ software.

ImageJ was utilized to calculate the cross-sectional area and thickness of the stria vascularis on mid-modiolar sections. Fluorescence intensity quantification was performed in ImageJ by calculating the fluorescence intensity of the outlined region of the stria vascularis on maximal intensity projection images. Fluorescence intensity was normalized by comparing the stria vascularis fluorescence intensity to that of a corresponding region in the scala media. Measurements were made for upper, middle, and lower turns of the cochlea for Kcnq1, Slc12a2, Kcnj10 and Cldn11 markers.

Statistical Analyses

Unless stated otherwise, all values represent mean ± SEM. For pairwise comparisons (between wt and a given mutant), an unpaired 2-tailed Student’s t-test was used. For comparison of multiple genotype groups, ANOVAs were performed. For analysis of auditory thresholds, a one-way ANOVA followed by a Bonferroni’s posthoc t-test was used for each stimulus frequency. For comparison of hair cell and spiral ganglion cell numbers in wt and dko groups over developmental time or with T3 treatment, a two-way ANOVA followed by a Bonferroni’s posthoc t-test was used. For consistency with previous analyses of T4 and T3⁵⁸, T4 and T3 levels were compared across genotypes using a two-way ANOVA and Tukey’s post hoc analysis. All statistical analyses were performed using GraphPad Prism version 6.0 h for Mac. The datasets generated and/or analyzed during the current study are available from the corresponding authors on reasonable request.

References

Heuer, H. & Visser, T. J. The pathophysiological consequences of thyroid hormone transporter deficiencies: Insights from mouse models. Biochim Biophys Acta 1830, 3974–3978 (2013).
Article CAS PubMed Google Scholar
Schweizer, U. & Kohrle, J. Function of thyroid hormone transporters in the central nervous system. Biochim Biophys Acta 1830, 3965–3973 (2013).
Article CAS PubMed Google Scholar
Dumitrescu, A. M., Liao, X. H., Best, T. B., Brockmann, K. & Refetoff, S. A novel syndrome combining thyroid and neurological abnormalities is associated with mutations in a monocarboxylate transporter gene. Am J Hum Genet 74, 168–175 (2004).
Article CAS PubMed Google Scholar
Friesema, E. C. et al. Association between mutations in a thyroid hormone transporter and severe X-linked psychomotor retardation. Lancet 364, 1435–1437 (2004).
Article CAS PubMed Google Scholar
Friesema, E. C. et al. Identification of monocarboxylate transporter 8 as a specific thyroid hormone transporter. J Biol Chem 278, 40128–40135 (2003).
Article CAS PubMed Google Scholar
van der Deure, W. M., Peeters, R. P. & Visser, T. J. Molecular aspects of thyroid hormone transporters, including MCT8, MCT10, and OATPs, and the effects of genetic variation in these transporters. J Mol Endocrinol 44, 1–11 (2010).
Article PubMed Google Scholar
Mayerl, S. et al. Transporters MCT8 and OATP1C1 maintain murine brain thyroid hormone homeostasis. J Clin Invest 124, 1987–1999 (2014).
Article CAS PubMed PubMed Central Google Scholar
Wirth, E. K. et al. Neuronal 3′,3,5-triiodothyronine (T3) uptake and behavioral phenotype of mice deficient in Mct8, the neuronal T3 transporter mutated in Allan-Herndon-Dudley syndrome. J Neurosci 29, 9439–9449 (2009).
Article CAS PubMed Google Scholar
Ng, L., Kelley, M. W. & Forrest, D. Making sense with thyroid hormone–the role of T(3) in auditory development. Nat Rev Endocrinol 9, 296–307 (2013).
Article CAS PubMed Google Scholar
DeLong, G. R., Stanbury, J. B. & Fierro-Benitez, R. Neurological signs in congenital iodine-deficiency disorder (endemic cretinism). Dev Med Child Neurol 27, 317–324 (1985).
Article CAS PubMed Google Scholar
Ferrara, A. M. et al. Homozygous thyroid hormone receptor beta-gene mutations in resistance to thyroid hormone: three new cases and review of the literature. J Clin Endocrinol Metab 97, 1328–1336 (2012).
Article CAS PubMed PubMed Central Google Scholar
Rovet, J., Walker, W., Bliss, B., Buchanan, L. & Ehrlich, R. Long-term sequelae of hearing impairment in congenital hypothyroidism. J Pediatr 128, 776–783 (1996).
Article CAS PubMed Google Scholar
Lichtenberger-Geslin, L. et al. Factors associated with hearing impairment in patients with congenital hypothyroidism treated since the neonatal period: a national population-based study. J Clin Endocrinol Metab 98, 3644–3652 (2013).
Article CAS PubMed Google Scholar
Bradley, D. J., Towle, H. C. & Young, W. S. 3rd. Alpha and beta thyroid hormone receptor (TR) gene expression during auditory neurogenesis: evidence for TR isoform-specific transcriptional regulation in vivo. Proc Natl Acad Sci USA 91, 439–443 (1994).
Deol, M. S. An experimental approach to the understanding and treatment of hereditary syndromes with congenital deafness and hypothyroidism. J Med Genet 10, 235–242 (1973).
Article CAS PubMed PubMed Central Google Scholar
Uziel, A. Periods of sensitivity to thyroid hormone during the development of the organ of Corti. Acta Otolaryngol Suppl 429, 23–27 (1986).
Article CAS PubMed Google Scholar
Dettling, J. et al. Autonomous functions of murine thyroid hormone receptor TRalpha and TRbeta in cochlear hair cells. Mol Cell Endocrinol 382, 26–37 (2014).
Article CAS PubMed Google Scholar
Griffith, A. J. et al. Knock-in mouse model for resistance to thyroid hormone (RTH): an RTH mutation in the thyroid hormone receptor beta gene disrupts cochlear morphogenesis. J Assoc Res Otolaryngol 3, 279–288 (2002).
Article PubMed PubMed Central Google Scholar
Rusch, A. et al. Retardation of cochlear maturation and impaired hair cell function caused by deletion of all known thyroid hormone receptors. J Neurosci 21, 9792–9800 (2001).
CAS PubMed Google Scholar
Mustapha, M. et al. Deafness and permanently reduced potassium channel gene expression and function in hypothyroid Pit1dw mutants. J Neurosci 29, 1212–1223 (2009).
Article CAS PubMed Google Scholar
Ng, L. et al. Age-Related Hearing Loss and Degeneration of Cochlear Hair Cells in Mice Lacking Thyroid Hormone Receptor beta1. Endocrinology 156, 3853–3865 (2015).
Article CAS PubMed PubMed Central Google Scholar
Ng, L. et al. Hearing loss and retarded cochlear development in mice lacking type 2 iodothyronine deiodinase. Proc Natl Acad Sci USA 101, 3474–3479 (2004).
Article ADS CAS PubMed PubMed Central Google Scholar
Axelsson, A. Comparative anatomy of cochlear blood vessels. Am J Otolaryngol 9, 278–290 (1988).
Article CAS PubMed Google Scholar
Campos-Barros, A. et al. Type 2 iodothyronine deiodinase expression in the cochlea before the onset of hearing. Proc Natl Acad Sci USA 97, 1287–1292 (2000).
Article ADS CAS PubMed PubMed Central Google Scholar
Friesema, E. C. et al. Effective cellular uptake and efflux of thyroid hormone by human monocarboxylate transporter 10. Mol Endocrinol 22, 1357–1369 (2008).
Article CAS PubMed PubMed Central Google Scholar
Sharlin, D. S., Visser, T. J. & Forrest, D. Developmental and cell-specific expression of thyroid hormone transporters in the mouse cochlea. Endocrinology 152, 5053–5064 (2011).
Article CAS PubMed PubMed Central Google Scholar
Deol, M. S. Congenital deafness and hypothyroidism. Lancet 2, 105–106 (1973).
Article CAS PubMed Google Scholar
Hadj-Sahraoui, N., Seugnet, I., Ghorbel, M. T. & Demeneix, B. Hypothyroidism prolongs mitotic activity in the post-natal mouse brain. Neurosci Lett 280, 79–82 (2000).
Article CAS PubMed Google Scholar
Muller, J. et al. Tissue-specific alterations in thyroid hormone homeostasis in combined Mct10 and Mct8 deficiency. Endocrinology 155, 315–325 (2014).
Article PubMed Google Scholar
Ferrara, A. M. et al. Changes in thyroid status during perinatal development of MCT8-deficient male mice. Endocrinology 154, 2533–2541 (2013).
Article CAS PubMed PubMed Central Google Scholar
Lukashkin, A. N., Richardson, G. P. & Russell, I. J. Multiple roles for the tectorial membrane in the active cochlea. Hear Res 266, 26–35 (2010).
Article PubMed Google Scholar
Johnson, K. R. et al. Congenital hypothyroidism, dwarfism, and hearing impairment caused by a missense mutation in the mouse dual oxidase 2 gene, Duox2. Mol Endocrinol 21, 1593–1602 (2007).
Article CAS PubMed Google Scholar
Wangemann, P. Supporting sensory transduction: cochlear fluid homeostasis and the endocochlear potential. J Physiol 576, 11–21 (2006).
Article CAS PubMed PubMed Central Google Scholar
Peeters, R. P., Ng, L., Ma, M. & Forrest, D. The timecourse of apoptotic cell death during postnatal remodeling of the mouse cochlea and its premature onset by triiodothyronine (T3). Mol Cell Endocrinol 407, 1–8 (2015).
Article CAS PubMed PubMed Central Google Scholar
Mariotta, L. et al. T-type amino acid transporter TAT1 (Slc16a10) is essential for extracellular aromatic amino acid homeostasis control. J Physiol 590, 6413–6424 (2012).
Article CAS PubMed PubMed Central Google Scholar
Kim, D. K. et al. Expression cloning of a Na+− independent aromatic amino acid transporter with structural similarity to H+/monocarboxylate transporters. J Biol Chem 276, 17221–17228 (2001).
Article CAS PubMed Google Scholar
Abe, S. et al. Monocarboxylate transporter 10 functions as a thyroid hormone transporter in chondrocytes. Endocrinology 153, 4049–4058 (2012).
Article CAS PubMed Google Scholar
Johannes, J. et al. Few Amino Acid Exchanges Expand the Substrate Spectrum of Monocarboxylate Transporter 10. Mol Endocrinol 30, 796–808 (2016).
Article CAS PubMed PubMed Central Google Scholar
Halestrap, A. P. The monocarboxylate transporter family–Structure and functional characterization. IUBMB Life 64, 1–9 (2012).
Article CAS PubMed Google Scholar
Kikuchi, T., Kimura, R. S., Paul, D. L., Takasaka, T. & Adams, J. C. Gap junction systems in the mammalian cochlea. Brain Res Brain Res Rev 32, 163–166 (2000).
Article CAS PubMed Google Scholar
Jagger, D. J. & Forge, A. Connexins and gap junctions in the inner ear–it’s not just about K(+) recycling. Cell Tissue Res 360, 633–644 (2015).
Article CAS PubMed Google Scholar
Bernal, J., Guadano-Ferraz, A. & Morte, B. Thyroid hormone transporters–functions and clinical implications. Nat Rev Endocrinol 11, 506 (2015).
Article PubMed Google Scholar
Schwartz, C. E. et al. Allan-Herndon-Dudley syndrome and the monocarboxylate transporter 8 (MCT8) gene. Am J Hum Genet 77, 41–53 (2005).
Article CAS PubMed PubMed Central Google Scholar
Van Eyken, E., Van Camp, G. & Van Laer, L. The complexity of age-related hearing impairment: contributing environmental and genetic factors. Audiol Neurootol 12, 345–358 (2007).
Article PubMed Google Scholar
Groves, A. K. The challenge of hair cell regeneration. Exp Biol Med (Maywood) 235, 434–446 (2010).
Article CAS Google Scholar
Gil-Ibanez, P., Morte, B. & Bernal, J. Role of thyroid hormone receptor subtypes alpha and beta on gene expression in the cerebral cortex and striatum of postnatal mice. Endocrinology 154, 1940–1947 (2013).
Article PubMed Google Scholar
Marcus, D. C., Wu, T., Wangemann, P. & Kofuji, P. KCNJ10 (Kir4.1) potassium channel knockout abolishes endocochlear potential. Am J Physiol Cell Physiol 282, C403–407 (2002).
Article CAS PubMed Google Scholar
Scholl, U. I. et al. Seizures, sensorineural deafness, ataxia, mental retardation, and electrolyte imbalance (SeSAME syndrome) caused by mutations in KCNJ10. Proc Natl Acad Sci USA 106, 5842–5847 (2009).
Article ADS CAS PubMed PubMed Central Google Scholar
Liu, H. et al. Organ of Corti and Stria Vascularis: Is there an Interdependence for Survival? PLoS One 11, e0168953 (2016).
Article PubMed PubMed Central Google Scholar
Sundaresan, S., Balasubbu, S. & Mustapha, M. Thyroid hormone is required for the pruning of afferent type II spiral ganglion neurons in the mouse cochlea. Neuroscience 312, 165–178 (2016).
Article CAS PubMed Google Scholar
Friauf, E. et al. Hypothyroidism impairs chloride homeostasis and onset of inhibitory neurotransmission in developing auditory brainstem and hippocampal neurons. Eur J Neurosci 28, 2371–2380 (2008).
Article PubMed Google Scholar
Trajkovic, M. et al. Abnormal thyroid hormone metabolism in mice lacking the monocarboxylate transporter 8. J Clin Invest 117, 627–635 (2007).
Article CAS PubMed PubMed Central Google Scholar
Ng, L. et al. A protective role for type 3 deiodinase, a thyroid hormone-inactivating enzyme, in cochlear development and auditory function. Endocrinology 150, 1952–1960 (2009).
Article CAS PubMed Google Scholar
Noguchi, T., Sugisaki, T., Satoh, I. & Kudo, M. Partial restoration of cerebral myelination of the congenitally hypothyroid mouse by parenteral or breast milk administration of thyroxine. J Neurochem 45, 1419–1426 (1985).
Article CAS PubMed Google Scholar
Wangemann, P. et al. Loss of KCNJ10 protein expression abolishes endocochlear potential and causes deafness in Pendred syndrome mouse model. BMC Med 2, 30 (2004).
Article PubMed PubMed Central Google Scholar
Wangemann, P. et al. Loss of cochlear HCO3- secretion causes deafness via endolymphatic acidification and inhibition of Ca2+ reabsorption in a Pendred syndrome mouse model. Am J Physiol Renal Physiol 292, F1345–1353 (2007).
Article CAS PubMed PubMed Central Google Scholar
Friedrichsen, S. et al. Regulation of iodothyronine deiodinases in the Pax8−/− mouse model of congenital hypothyroidism. Endocrinology 144, 777–784 (2003).
Article CAS PubMed Google Scholar
Muller, J. & Heuer, H. Expression pattern of thyroid hormone transporters in the postnatal mouse brain. Front Endocrinol (Lausanne) 5, 92 (2014).
Google Scholar
Iwagaki, T., Suzuki, T. & Nakashima, T. Development and regression of cochlear blood vessels in fetal and newborn mice. Hear Res 145, 75–81 (2000).
Article CAS PubMed Google Scholar

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Acknowledgements

This work was supported by the Intramural Research Program at NIDDK (DSS, LN, YL, DF) and intramural grant DC000088-01 at NIDCD (RO, MH) at the National Institutes of Health, DFG grants SPP1629/He3418/7-1 and 7-2 (HH), the Swiss National Science Foundation (FV) and faculty funds at Minnesota State University Mankato (DSS).

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David S. Sharlin and Lily Ng contributed equally to this work.

Authors and Affiliations

Department of Biological Sciences, Minnesota State University Mankato, Mankato, Minnesota, 56001, USA
David S. Sharlin
Laboratory of Endocrinology and Receptor Biology, NIDDK, National Institutes of Health, Bethesda, Maryland, 20892, USA
David S. Sharlin, Lily Ng, Ye Liu & Douglas Forrest
Center for Integrative Human Physiology (ZIHP) and NCCR Kidney. CH, Institute of Physiology, University of Zürich, Zürich, 8057, Switzerland
François Verrey
Department of Internal Medicine and Rotterdam Thyroid Center, Erasmus University Medical Center, Rotterdam, The Netherlands
Theo J. Visser
National Institute on Deafness and other Communication Disorders, National Institutes of Health, Bethesda, Maryland, 20892, USA
Rafal T. Olszewski & Michael Hoa
Department of Endocrinology, Diabetes and Metabolism, University Hospital Essen, University of Duisburg-Essen, 45147, Essen, Germany
Heike Heuer

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D.S.S., L.N., H.H., T.J.V. and R.O. performed the research; D.S.S., L.N., H.H., Y.L., R.O., M.H., D.F. designed research and analyzed data; F.V. provided reagents; D.S.S., L.N., M.H. and D.F. wrote the paper.

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Sharlin, D.S., Ng, L., Verrey, F. et al. Deafness and loss of cochlear hair cells in the absence of thyroid hormone transporters Slc16a2 (Mct8) and Slc16a10 (Mct10). Sci Rep 8, 4403 (2018). https://doi.org/10.1038/s41598-018-22553-w

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Received: 20 July 2017
Accepted: 16 February 2018
Published: 13 March 2018
DOI: https://doi.org/10.1038/s41598-018-22553-w

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