Figure 11 | Scientific Reports

Figure 11

From: In silico screening for human norovirus antivirals reveals a novel non-nucleoside inhibitor of the viral polymerase

Figure 11

Secondary screen for inhibition of human norovirus Sydney 2012 RdRp activity. (A) The inhibitory effects of 26 compounds were examined against norovirus RdRp activity using a fluorescent activity assay at 10 µM. Five compounds that exhibited more than 50% inhibition (red bars) were chosen for further assessment with a counter-screen gel-shift RdRp assay. The positive control (blue bar) is the norovirus NNI RdRp inhibitor NIC0214. The mean values of triplicate datasets with standard deviations are shown. (B) A counter-screen-gel-shift assay was used to confirm norovirus RdRp inhibitory activity of the five compounds identified in panel (A). PE44-NoV RNA templates (32 nucleotides) were extended (44 nucleotides) by the RdRp in the absence of any test compounds (0.5% DMSO [vol/vol] negative control) or with test compounds at a fixed concentration of 100 µM. The nucleoside analogue 3′-deoxyguanosine-5′-triphosphate (3′dGTP) was used as a positive control (10 µM) to demonstrate complete inhibition, and no RdRp was used as a negative control. Full-length gels are presented in Supplementary Fig. S3.

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