Predicted changes in PI(4,5)P2 and PI(3,4)P2 levels as a consequence of siRNA knockdown assays. Each kinase is inactivated, one at the time, and phosphatases are upregulated. The protein complex catalysing v0→45 is composed of PIP5KI and PI4K; therefore, when one of these enzymes is knocked out, the complex should be also knocked out. There is also the possibility of knocking out only the complex. Enzymes are coloured according to the classification in the siRNA screens: enzymes activating ENaC are marked red, those inhibiting ENaC are marked green and those exhibiting both effects are marked black. The graph was created in R42 and the x axis labels were added with PowerPoint.