PI(3,4,5)P3 is sensitive to PTEN when v345→34 is slow. A decrease in PTEN is sufficient to increase the levels of PI(3,4,5)P3 and change the membrane configuration form apical (low PI(3,4,5)P3) to basolateral (high PI(3,4,5)P3). A fast v345→34 will decrease PI(3,4,5)P3 and make the membrane much less sensitive to a PTEN change. A PTEN knockdown of 98.33% does not alter the amount of PI(4,5)P2 in either fast or slow v345→34 conditions. Fast v345→34 increases the levels of PI(3,4)P2 and makes the levels of this lipid dependent on the PI(3,4,5)P3 pool. Slow v345→34 is modelled as γ345→34 = 1e11 and f345→34 = 0.9982. Fast v345→34 is modelled as γ345→34 = 6e13 and f345→34 = 0.9998. The graph was created in R42 and the x axis labels were added with PowerPoint.