Figure 5 | Scientific Reports

Figure 5

From: ASC- and caspase-8-dependent apoptotic pathway diverges from the NLRC4 inflammasome in macrophages

Figure 5

Caspase-1 does not compete with ASC for interaction with NLRC4 in enzymatically inactive Casp1C284A/C284A BMDMs. (a) Immunoblot of pro-caspase-1 and actin from wt, Casp1−/−, Casp1C284A/C284A BMDMs. (b) BMDMs with Pam3CSK4 (1 μg ml−1) pre-stimulation were electroporated with flagellin (0.5 μg ml−1). IL-1β release and LDH release measured after 16 h. Data is expressed as mean ± SD; n = 3. (c) % YOYO-1 positive BMDMs from two mice/genotype after stimulation. BMDMs with no pre-stimulation were electroporated with flagellin (0.5 μg ml−1). Live cell images taken every 45 min for 16 h.

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