Figure 4 | Scientific Reports

Figure 4

From: ASC- and caspase-8-dependent apoptotic pathway diverges from the NLRC4 inflammasome in macrophages

Figure 4

NF-κB signaling blocks NLRC4/caspase-8-dependent apoptosis pathway. Casp1−/−Casp11−/− BMDMs were pre-stimulated for with or without various stimuli – Pam3CSK4 (1 μg ml−1), LPS (1 μg ml−1), R837 (2 μg ml−1), TNFα (100 ng ml−1), IFN-α (100 U ml−1), or IFN-β (100 U ml−1), then electroporated with flagellin (0.5 μg ml−1) or stimulated with FasL (100 ng ml−1). (a) LDH release after 16 h. Data is represented as mean ± SD; n = 3. (b) % YOYO-1 positive BMDMs from live cell imaging taken every 45 min for 16 h.

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