ASC and caspase-8 are identified through a genome-wide CRISPR/Cas9 screen for caspase-1-independent NLRC4-mediated cell death. (a) % YOYO-1 positive iMac cell lines from live imaging taken every hour up to 16 h after flagellin electroporation. Data is represented as mean ± SD; n = 3 images. (b) Scatter plot showing relative fold-change enrichment of genes (x-axis) with their corresponding enrichment p value (y-axis) from n = 3 biological replicates. Counts are log2 transformed and normalized using median scaling. Top scoring genes are highlighted. (c) Box-plot showing the distribution of individual sgRNA frequencies of flagellin- over control-treated populations, ordered left-to-right by increasing p-value.