Figure 5 | Scientific Reports

Figure 5

From: Vesicle-based secretion in schistosomes: Analysis of protein and microRNA (miRNA) content of exosome-like vesicles derived from Schistosoma mansoni

Figure 5

Quantitative qRT-PCR analysis of S. mansoni exosomal miRNAs in sera of infected mice. Circulating exosomes were purified from sera of S. mansoni –infected mice at 6–7 weeks post-infection or uninfected controls of the same age, using ExoQuick. RNA was extracted from the purified exosomes and then used for amplification of four S. mansoni miRNAs (Sma-mir-125a, Sma-mir-125b, Sma-mir-71a, Sma-bantam) by qRT-PCR. Data were median normalized relative to a “spike-in” synthetic miRNA17,80 and are shown as the fold-change relative to the uninfected control sample (background).

Back to article page