Figure 5 | Scientific Reports

Figure 5

From: Intratesticular injection followed by electroporation allows gene transfer in caprine spermatogenic cells

Figure 5

Detection of EGFP expression in embryos produced in vitro using semen from in vivo gene transferred bucks. (A) In vitro produced embryos (pooled). Scale bar, 100 μm; (B) EGFP expression in the embryo (a) under bright-field and (b) UV light. Scale bar, 50 μm; (C) RT-PCR analysis revealed expression of EGFP mRNA in the fluorescent embryo. 1; normal embryo, 2; EGFP-expressing embryo, 3; 100 bp molecular marker. The gel picture was cropped, and the full-length gel is presented in Supplementary Figure 5C.

Back to article page