Assessment of semen samples obtained from bucks with transfected testis. (A) The semen parameters showed no variation before and after gene transfer. Semen from three in vivo gene transferred bucks (n = 3) were assessed at different intervals. BE: before electroporation, MY: progressive motility, VY: viability, MI: membrane integrity, AI: acrosome integrity. Data represent the mean ± s.e.m.; (B) EGFP expression in the sperm from a gene transferred buck; (C) no expression of EGFP was observed in sperm from a wild buck. (a & c): Bright-field, (b & d): under UV light. Scale bar, 20 μm; (D) The graph shows qPCR analysis of sperm from in vivo gene transferred bucks at different intervals indicating the genomic integration of transgene. Data represent the mean ± s.e.m. for n = 3. Here, ‘ns’ means statistically not significant.