Figure 3 | Scientific Reports

Figure 3

From: Intratesticular injection followed by electroporation allows gene transfer in caprine spermatogenic cells

Figure 3

EGFP expression in the testis of pre-pubertal bucks on d21 after in vivo gene transfer. (A) Gel electrophoresis of quantitative real-time PCR products from gene transferred testis revealed an EGFP-specific band of 104 bp. The top of the gel with the wells was cropped; N: no template control (NTC), G1-G3: testicular tissue from pre-pubertal goats with gene transferred testis, S1 & S2: cultured seminiferous tubules from in vitro transfected testes, P: positive control (pIRES2-EGFP plasmid), M: 100 bp molecular marker; (B) graphical representation of the relative expression of EGFP mRNA in testicular tissues from pre-pubertal bucks at d21 post-gene transfer. G1, G2 & G3 are pre-pubertal bucks #1, #2 & #3, respectively. Data represent the mean ± s.e.m. for n = 3. Here, ‘ns’ means statistically not significant; (C) an approx. 27 kDa protein band was observed by western blotting analysis, using total protein from testicular tissue of an in vivo electroporated goat. The marker lane was cut separately before incubating the membrane with the primary antibody. The wells are labeled 1: gene transferred testis of a pre-pubertal goat, 2: testis of a non-gene transferred goat, 3: protein marker (#1610373, Bio-Rad).