Figure 2 | Scientific Reports

Figure 2

From: Intratesticular injection followed by electroporation allows gene transfer in caprine spermatogenic cells

Figure 2

Expression of EGFP in testicular cells on d21 after in vivo gene transfer. (A) Direct fluorescence microscopy of a testis cross-section showing green fluorescence in testicular cells; (B) IHC revealed the localization EGFP protein (in brown) in almost all the testicular cells including spermatogonial cells; (C) clusters of green fluorescent cells in seminiferous tubules; (D) IHC analysis of testis showing the location of SSCs in the basement membrane of seminiferous tubules. The α6 integrin antibody was used as a specific marker for SSCs. Scale bar, 50 μm.