Figure 1 | Scientific Reports

Figure 1

From: Intratesticular injection followed by electroporation allows gene transfer in caprine spermatogenic cells

Figure 1

EGFP expression in the goat testes transfected in vitro. EGFP expression was observed as green fluorescence in (A) cultured seminiferous tubules and (B) spermatogonial stem cell (SSC) colonies; (C) An absence of fluorescence was observed in cultured seminiferous tubules from the testis injected with only PBS (negative control). BF: Bright field, UV: Under UV light. Scale bar, 50 μm; (D) Graphical representation of transfection efficiency using different plasmid concentrations (0.1, 0.25, 0.5, 1.0 and 1.5 µg) in goat testis. After in vitro transfection, seminiferous tubules were isolated and cultured in 24-well plates. The X-axis denotes plasmid concentration and the Y-axis represents the number of wells showing EGFP expression in seminiferous tubules. P: PBS injection only, D: injection of plasmid DNA without electroporation and E + D: injection of plasmid DNA followed by electroporation. Data represent the mean ± s.e.m. for n = 3. Letters (a–d) above the bars indicate that these groups differ significantly. In the E + D, P < 0.05 (between 0.1 µg and 0.25 µg), P < 0.05 (between 0.25 µg and 0.5 µg) and P < 0.01 (between 0.5 µg and 1.0 µg). Between the D and E + D groups, ***P < 0.001.