Allopurinol-treated mice are partially protected against hindlimb unloading-induced skeletal muscle atrophy. (A) Hematoxylin and eosin staining (Original magnification, 4×. Scale bar, 100.00 µm). (B) Myosin staining specific of MHC (type I fibers were deeply stained, while type II were lightly stained. Original magnification 20×. Scale bar, 100.00 µm). (C) Shows quantitative analysis of the cross-sectional area of the soleus muscle. (D) Representative western blotting and densitometric analysis of soleus MHC I (full-length blots are included in supplementary information. See Supplementary Figure 1). Actin was used as a loading control. Data are shown as mean ± SD. C = Control (n = 5–7); U = Unloading (n = 5–7); UA = Unloading treated with allopurinol (n = 5–7).