Figure 2 | Scientific Reports

Figure 2

From: The selective reversible FAAH inhibitor, SSR411298, restores the development of maladaptive behaviors to acute and chronic stress in rodents

Figure 2

(A) Inhibitory effect of SSR411298 on mouse brain FAAH. Brain homogenates were incubated with 10 μM of [3H]anandamide in the absence (controls) or presence of increasing concentrations of SSR411298 (3 × 10−11 M to 10−5 M). Data are the mean ± SEM of 4 independent experiments; (B) Reversibility of the effects of SSR411298 and PMSF on mouse brain FAAH activity by dialysis. For each concentration, data are the mean ± SEM of mouse brain FAAH activity estimated by measuring [3H]ethanolamine production, expressed in percent of mean of controls after 16 to 18 hours at 25 °C without (black bars) or with dialysis (hatched bars). *P < 0.05 versus non dialyzed (Student’s t-test); (C) Ex vivo effect of SSR411298 on mouse brain FAAH activity 2 hours after intraperitoneal or oral administration; (D) Time-course of p.o. treatment with SSR411298 on mouse brain FAAH activity. Each point represents the mean ± SEM of 2 to 5 independent experiments. [3H]ethanolamine production are expressed as percent of mean of controls (vehicle treated mice). ID50 are calculated on specific [3H]ethanolamine production (equal to the total minus non-specific production).