Abstract
Conopomorpha sinensis Bradley is a host-specific pest of Litchi chinensis and Euphoria longan. Here, we demonstrated that C. sinensis has evolved special physical and chemical mechanisms for host plant location that enable it to survive and reproduce. Females favored laying their eggs on the convex surface of litchi fruit that had particular volatile characteristics. Experiments using a H-type olfactometer showed that female C. sinensis were attracted to litchi flowers, tender shoots, immature fruits, and mature fruits, with the highest attraction rate to mature fruits (74.67 ± 2.31%). There were no significant differences in the attraction of male C. sinensis to different litchi tissues. Further oviposition preference tests using the pericarp, pulp, and seeds of mature litchi fruits revealed that female C. sinensis prefer to lay their eggs on the pericarp. Litchi volatiles were found to be important in attracting C. sinensis to fruits for oviposition. Analysis of volatiles from different litchi tissues by HS-SPME-GC-MS revealed 31 similar volatiles, some of which may be important in the oviposition preference choices of C. sinensis on litchi fruit.
Similar content being viewed by others
Introduction
Conopomorpha sinensis Bradley (Lepidoptera: Gracilariidae) is an important pest of Litchi chinensis and Euphoria longan1. Its larvae can bore into litchi fruit, damaging flowers, tender shoots, and leaves. After hatching, the larvae immediately penetrate the fruits, feeding on the seed neck, and can transfer a variety of microorganisms that infect the fruits and eventually destroy the crop2,3. This has caused severe economic loss to the Chinese litchi industry, with disastrous consequences for the export of Litchi and longan fruit4. Due to its cryptic feeding behavior and overlapping of generations, C. sinensis is difficult to control. Difficulties experienced with chemical control have led researchers to explore the use of biological and molecular approaches to controlling this pest5,6,7.
Although chemicals and physical irradiation can control C. sinensis8,9,10, biological control, which is one of the most widely used environmentally benign approaches to controlling pests11, has focused on the natural enemies of C. sinensis. These include many species of spider, green lacewings, predatory mirids, ladybugs, ants, and parasitic wasps6,12. Plant protectants and biopesticides can also control C. sinensis13,14. Techniques based on chemical ecology have received less attention, except for primary screens of plant volatiles to repel or lure C. sinensis15,16,17,18. Whether the attraction of C. sinensis to litchi is mediated via behavior and chemoreception is unknown.
The selection and adaptation of insects to their hosts are the result of co-evolution19,20,21,22. Both the highly sensitive olfactory system of the insect and the odor of the host plant play vital roles in insect survival and reproduction23. Studying the mechanisms underlying chemical communication between insects and plants can provide a target for the development of highly efficient and specific regulators of insect behavior24. The aims of the present study were to identify the factors responsible for the selection of litchi as a suitable host by C. sinensis, and to screen for host volatiles capable of luring C. sinensis. This study will be useful in the development of insect attractants for field biological control and forecasting insect outbreaks. A better understanding of the chemical ecology of litchi and C. sinensis will help to provide a green biocontrol technology with the characteristics of non-toxicity, non-pollution, and sustainability.
Results
The choice behavior of C. sinensis depends on the surface characteristics and volatiles of host plants and non-host plants
The taxis behavior response of C. sinensis to the surface of host plants and differences in their responses to host plants and non-hosts were observed. There were significant differences in oviposition choice by female C. sinensis on different surfaces of host plants, which preferred to oviposit on the convex surface of host plants (F = 37.14, df = 3, 12, P < 0.05). The average number of eggs deposited on the convex surface of host plants was 30.33 ± 2.52, which was similar to that of the litchi fruit control (32.00 ± 2.00) (Fig. 1). The second favorite oviposition site was the concave surface of host plants. Female C. sinensis preferred host plants to non-host plants (F = 20.14, df = 3, 12, P < 0.05), which was similar to that of the control group (Fig. 2). Otherwise, there were fewer eggs, and even no difference in oviposition between non-host plants and non-host plastic balls. This demonstrates that the surface physical characteristics and volatiles of litchi had effects on the oviposition of C. sinensis.
Taxis reactions of C. sinensis to different tissues of litchi
In this study, the taxis behavior of adult C. sinensis to different litchi tissues was studied using a H-type olfactometer13,25. The results showed differences in the taxis behavior of female and male adults of C. sinensis to different tissues of the litchi host (Table 1), with female adults preferring the host more than male adults. The attraction rates of mature fruits to female adult C. sinensis was 74.67 ± 2.31%, which had a significant attraction than other tissues of host plants (F = 44.98, df = 3, 12, P < 0.05). The attraction rate of male adult C. sinensis to different litchi tissues was less than 27%, although this was not statistically significant (F = 4.44, df = 3, 12, P > 0.05).
Oviposition preference of female C. sinensis to different tissues of litchi fruit
The oviposition preference test showed that the number and proportion of laid eggs on pericarp tissues were the largest among all the treatments (Fig. 3). Female adults laid 175 ± 28 eggs on average and 88.83% in total on the pericarp, which is significantly different to the values obtained with pulps and seeds of litchi fruit (F = 10.93, df = 3, 9, P < 0.05).
Isolation and identification of volatiles from different litchi tissues
Results of HS-SPME-GC-MS analysis indicated that the volatiles of different litchi tissues were diverse. Figure 4 shows representative chromatograms of litchi: (a) flowers, (b) tender shoots, (c) immature fruits, and (d) mature fruits. The numbers of chromatographic peaks for each tissue type were 17, 15, 12, and 28, respectively, and 42 volatile compounds were identified. A database comparison of mass spectra revealed 31 similar volatiles (Similarity, SI >800; Table 2). The individual chemical compounds and their percentage in the volatile emissions of different litchi tissues are shown in Table 2. The analysis showed that the major compounds of the volatiles from different litchi tissues were terpenes (21%), alcohols (3%), and esters (2%). There were also large differences among the volatiles: litchi flowers contained caryophyllene (25.11%), (+)-aromadendrene (18.70%), α-copaene (10.76%); volatiles from tender shoots contained ß-elemene (38.7%), (+)-aromadendrene (29.18%), (+)-epi-bicyclosesquiphellandrene (13.48%); immature fruit contained mainly 1,3-cyclohexadiene, 5-(1,5-dimethyl-4-hexenyl)−2-methyl-, [S-(R*,S*)]- (39.01%), l-ß-bisabolene (27.06%), benzene, 1-(1,5-dimethyl-4-hexenyl)−4-methyl- (9.02%); and mature fruit contained mainly 1,3-cyclohexadiene, 5-(1,5-dimethyl-4-hexenyl)−2-methyl-, [S-(R*,S*)]- (35.21%), l-ß-bisabolene (27.75%), and caryophyllene (9.71%). The volatile common to the different litchi tissues was (+)-aromadendrene, which was found in the following proportions: tender shoots (29.18%)> flowers (18.70%)> immature fruits (8.41%)> mature fruits (0.59%).
Discussion
The relationship between insects and plants is mainly a reflection of insect feeding, oviposition, and choice of habitat26. The host range of C. sinensis is narrow, and there is high host specificity. To date, C. sinensis has only been reported in Litchi chinensis and Euphoria longan in South China and Southeast Asia6,27,28. Previous studies show that the cultivar of Litchi chinensis most seriously affected by C. sinensis is Feizixiao15. In the present study, physical and chemical choice behavior tests confirmed that C. sinensis exhibited a preference for different tissues of the litchi cultivar Feizixiao. The host choice behavior of C. sinensis showed that female C. sinensis preferred to oviposit on a convex surface that resembled that of the surface of litchi fruit. Some researchers link this oviposition behavior to predator avoidance29,30,31. In addition, differences in the selection of host plants and non-hosts by C. sinensis confirmed that host volatiles attract C. sinensis to the oviposit. For many herbivorous insects, host plant selection is very important for progeny survival and fitness32.
The relationship between herbivorous insects and host plant compounds plays a vital role in the preference choices of insects to their host plants33,34,35. Different tissues and volatile cues from host plants are crucial for insect attraction, affecting choice of feeding sites36, oviposition behavior37, helping offspring to locate and recognize a suitable host plant for larval growth and movement38,39, and even finding a mating partner40. Behavioral assays in a H-type olfactometer showed that adult female C. sinensis preferred mature fruits to the other tissues of host plants. By contrast, there were no significant differences in the taxis reactions of male adult C. sinensis among the different litchi tissues. This suggests that host plant compounds and their nutritional quality might be key factors in offspring survival30,41,42,43. Tests of the oviposition preference of female C. sinensis to different tissues of mature litchi fruits indicated that the greatest number and proportion of eggs were laid on the pericarp. This is consistent with the results of Xian et al.13, who reported that C. sinensis preferred the pericarp of host plant fruits after mating.
Plants can produce complicated odor blends44, with large numbers of volatiles comprising several compounds in particular ratios. These volatiles strongly influence the ecological interactions of plants with insects45. In the present study, 42 compounds, many of which were terpenes, were identified in the volatile blend emitted from different litchi tissues (Table 2). These findings provide a theoretical basis for studying chemical communication between C. sinensis and litchi. Previous studies revealed that zingiberene attracts C. sinensis to lay eggs on its host18. (E)-ß-farnesene and (Z)-ß-farnesene were two unique compounds from mature fruit pericarp of the litchi cultivar Feizixiao, and it was speculated that specific ratios of these compounds and six other volatiles that were mainly found in representative susceptible cultivars of litchi may contribute to the attraction of C. sinensis46. Our results suggest that the chemical composition of mature litchi fruit is most conducive to successful attraction of adult female C. sinensis. We found that silanol, trimethyl-, 2-isopropyl-5-methyl-9-methylenebicyclo[4.4.0]dec-1-ene, isocaryophillene, α-trans-bergamotene, (+)-cyclosativene, and cyclohexene were unique compounds of the volatiles of mature Feizixiao fruit. In addition, 7-epizingiberene, l-ß-bisabolene, and caryophyllene were detected as the main compounds of the volatiles emitted from immature and mature fruit, while (+)-aromadendrene is the common compound of the volatiles from different litchi tissues. Volatile blends comprising these constituents in specific proportions may be key signals for host identification in C. sinensis. How the mature fruit processes such volatile mixtures is unclear and requires further research.
In conclusion, our studies indicate that C. sinensis has evolved physical and chemical mechanisms to locate a suitable host, thereby enabling it to survive and reproduce. Host plant volatiles have a significant effect on the oviposition preference of adult female C. sinensis, and the analysis and identification of these volatiles afford many valuable basal data and candidate volatiles, which are key factors in the choice of C. sinensis to oviposit on litchi (Fig. 5). To take advantage of the ability of host plant volatiles to influence directional selection in C. sinensis, it is necessary to determine the single and composite compounds of host volatiles that exert the greatest attractant activity to C. sinensis. Future work will require the implementation of electroantennogram (EAG) response measurements, wind tunnel tests, field tests, and molecular tools to tackle C. sinensis infestation effectively.
Methods
Insects and litchi fruits
Conopomorpha sinensis Bradley for olfactory experiments were collected from the litchi orchard, Institution of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, China. They were reared on litchi fruit in a climate room (26 ± 1 °C; 14 h Light:10 h Dark; 60–80% RH). Females and males were identified and separated according to their reproductive organs under a stereomicroscope.
Fresh fruits of the litchi cultivar Feizixiao were chosen as the basic material, since it is the cultivar most seriously affected by C. sinensis15. The litchi flower, tender shoot, fruit, and the fruit pericarp, pulp, seed were separated for experiments.
C. sinensis attraction to the surfaces of host plants and non-host plants Attraction of C. sinensis to the surfaces of host plants
Ten mature litchi fruits were wrapped individually in either flat paper, convex paper, or concave paper; controls were not wrapped in paper. The fruit were then placed in the same insect rearing cage (45 cm × 45 cm × 50 cm). Twenty 2-day-old male and female adults of C. sinensis (♀:♂ = 1:1) were placed in one cage for the oviposition experiment with three replicates; every insect was used for once in the test. After 48 h, the egg numbers oviposited by females from each treatment was recorded.
Attraction of C. sinensis to host plants and non-host plants
Ten each of the following were wrapped in convex paper: mature litchi fruits, fruits of a non-host plant (Prunus salicina Lindl, a kind of plum, the same size as litchi fruits), and non-host (red plastic balls of similar dimensions to litchi fruits). The wrapped fruits and plastic balls were placed in the same insect rearing cage (dimensions as above). The control consisted of the same number of mature litchi fruits, unwrapped. The oviposition test with three replicates were recorded as described above.
Chemical choice behavior of C. sinensis to host plants Taxis reaction of C. sinensis to different tissues of litchi
The taxis reaction of C. sinensis to four different litchi tissues (flower, tender shoots, immature fruit, and mature fruit) was studied using the H-type olfactometer method of Kudon25 and Xian et al.13. The olfactometer is in the form of letter H and made of plexiglass. It had two cylindrical channels (Height: 30 cm, Diameter:10 cm) on each side, and the channels plus a cylindrical bioassay chamber (Length: 30 cm, Diameter: 3 cm) composed the olfactometer proper. Insects were placed in the central entry of the bioassay chamber and their activity to which side were recorded in the olfactometer. One side of the H-type olfactometer contained tender shoots, immature fruit, or mature fruit; the other side of the olfactometer contained nothing and was a blank control. Host selection behaviors were observed and recorded 1 hour after an insect was placed in the H-type olfactometer. Fifty male and female adult C. sinensis were tested in the experiment, which was repeated three times. Every insect was used for once in the test. The number of C. sinensis showing a taxis reaction to the different litchi tissues was recorded and the attraction rate was calculated.
Oviposition preference of female C. sinensis on different tissues of litchi fruit
The oviposition preference of female C. sinensis was tested in an insect rearing cage (45 cm × 45 cm × 50 cm). Fifty 2-day-old male and female adult C. sinensis (♀:♂ = 1:1) were placed in the cage and fed with 5% honey, soaked into cotton. All tissues were wrapped in convex paper respectively. The cage was filled respectively with three litchi pericarps, pulps, seeds, and blank controls at random positions within the cage. Each experiment was performed in triplicate. The culture environment was at 26 ± 1 °C, 14 h:10 h (Light: Dark) photoperiod, and 60–80% RH. The number of ovipositing female C. sinensis was recorded after 48 h.
Analysis of volatiles from different litchi tissues using HS-SPME-GC-MS Headspace solid-phase microextraction (HS-SPME)
Fresh litchi fruits were put into a 500 ml extraction bottle and sealed. The SPME conditions were further optimized by careful selection of the fibers, the extraction time, and the desorption time according to Bianchin et al.47. After 1 h microextraction, the extraction fiber was placed into the instrument for GC-MS analysis. The temperature for SPME fiber desorption was 200 °C with 3 min sample injection.
GC-MS analysis
A gas chromatograph (GC-8000Top, Finnigan, USA) equipped with a gas chromatographic column (30 mm × 0.25 mm × 0.25 μm) was used during the study. The injector temperature was set at 240 °C and operated in split mode with a flow rate of 10 ml min−1. Ultrapure helium at 1 ml min−1 was used as the carrier gas. The oven temperature program was as follows: 40 °C (held for 4 min), 10 °C min−1 to 240 °C (held for 3 min). MS detection was performed under the following conditions: ionization source at 230 °C, and the ionization mode was electron impact with electron energy of 70 eV, 350 v. The mass spectrometer was operated in total scan mode over a m/z range of 35–350 amu. The analytes were univocally characterized on the basis of their retention times and their mass-to-charge ratios.
Statistical analysis
The attraction rate formula of taxis reaction of C. sinensis to ifferent tissues of litchi = (number attracted by the treatment - number attracted by the control)/(n = 50) × 100%.
Statistical analysis was performed using SPSS Statistics 22 software (IBM Corp., Armonk, NY, USA). One-way ANOVA with a post hoc LSD test was used. Results were considered statistically significant when p-values were <0.05.
The chemical compounds of different litchi tissue volatiles were determined by analyzing and comparing the mass spectra using the U.S. National Institute of Standards and Technology (NIST) spectrum library, and the retention times from standard mass spectrometry.
References
Yao, Z. W. & Liu, S. K. Two Gracillariid insects attacking litchi and longan. Acta. Entomol. Sin. 33, 207–212 (1990).
Thanh, V. N., Hai, D. A. & Lachance, M. A. Cryptococcus bestiolae and Cryptococcus dejecticola, two new yeast species isolated from frass of the litchi fruit borer Conopomorpha sinensis Bradley. FEMS. Yeast. Res. 6, 298–304 (2006).
Wang, S. S., Huang, S. S., Liang, G. W. & Zeng, L. The rearing and the laboratory population life table of litchi fruit borer (Conopomorpha sinensis Bradley). Acta. Ecol. Sin. 28, 0836–0841 (2008).
Yi, G. J., Wang, X. B. & Huo, H. Q. The litchi industry status in China and its export strategies. J. Fruit. Sci. 19, 188–190 (2002).
Chen, B. X., Dong, Y. Z. & Lu, H. Development of Kelü™ 15% alphacypermethrin· chlorpyrifos EC and its field trial to Conopomorpha sinensis. Guangdong. Agr. Sci. 7, 97–99 (2010).
Meng, X. et al. Molecular screening and predation evaluation of the key predators of Conopomorpha sinensis Bradley (Lepidoptera: Gracilariidae) in litchi orchards. B. Entomol. Res. 104, 243–250 (2014).
Meng, X., Hu, J. J., Liu, H., Ooyang, G. C. & Guo, M. F. Analysis of the transcriptome and olfaction-related genes of Conopomorpha sinensis Bradley (Lepidoptera: Gracilariidae). Acta. Entomol. Sin. 59, 823–830 (2016).
Hu, M.Y. et al. Studies on gamma irradiation of litchi stem-end borer larvae as quarantine treatment. J. Zhongkai Agr. Coll. 11: 56–61(998)
Cai, Y. C. & Chen, Z. T. Control effect of 25% DUTE EC Against Conopomorpha sinensis Bradley. Chin. J. Trop Agr. 28, 17–18 (2008).
Fu, H. H. et al. Development, reproduction and sexual competitiveness of Conopomorpha sinensis (Lepidoptera: Gracillariidae) gamma-irradiated as pupae and adults. Fla. Entomol. 99, 66–72 (2016).
Ren, S. X. & Chen, X. X. Biological Control. Ch. 8, 227–262 (China Agriculture Press, Beijing. 2012).
Winson, T., Liang, G. W., Liu, W. H. & Chen, Q. X. The new record of selecting effective species of egg parasitoids of Conopomorpha sinensis Bradley (Lepidoptera: Graillariidae). Nat. Enemies. Insects. 29, 6–11 (2007).
Xian, J. D., Wang, X. L., Liu, S. L. & Ling, G. W. Behavioral responses of Conopomorpha sinensis Bradley to different tissues of host plants. J. Environ. Entomol. 33, 456–460 (2011).
Yang, P., Xiong, J. J., Liu, D. G. & Huang, M. D. Studies on the safety of mineral spray oil and its control of Conopomorpha sinensis in litchi and longan orchard. Nat. Enemies. Insects. 24, 26–31 (2002).
Peng, H. H., Lu, Y. Y., Liang, G. W. & Zeng, L. Oviposition attraction effect of host plants to the litchi fruit borer Conopomorpha sinensis. Chin. Bull. Entomol. 44, 361–363 (2007).
Yang, C. L., Jiang, S. H. & Xu, H. H. Oviposition Repellent Effect of Secondary Substance from Twelve Plants on Conopomorpha sinensis Bradley. J. Huazhong Agr. Univ. 26, 316–318 (2007).
Wang, S. S., Liang, G. W., Zeng, L. & Huang, S. S. Analysis on volatile components of fresh litchi fruit. Xinjiang Agr. Sci. 47, 237–240 (2010).
Huang, L. L., Li, C. Y., Deng, X. Y. & Gu, W. Compared study about volatility chemical constituents of young leaf from Litchi Chinese Sonn. Guangdong Chem. Ind. 37, 128–134 (2010).
Karban, R. & Baldwin, I.T. Induced Responses to Herbivory. (The University of Chicago Press, Chicago. III. 1997).
Qin, J. D. & Wang, C. Z. The relation of interaction between insects and plants to evolution. Acta. Entomol. Sin. 44, 360–365 (2001).
Will, T. & van Bel, A. J. E. Physical and chemical interactions between aphids and plants. J. Exp. Bot. 57, 729–737 (2006).
Walling, L. L. Avoiding effective defenses: strategies employed by phloem-feeding insects. Plant. Physiol. 146, 859–866 (2008).
Pitts, R. T. & Zwiebel, J. L. Antennal sensilla of two female anopheline sibing species with differing host ranges. Malaria J. 5, 26–29 (2006).
Plettner, E. Insect pheromone olfaction: new targets for the design of species-selective pest control agents. Curr. Med. Chem. 9, 1075–1085 (2002).
Kudon, L. & Berisford, C. An olfactometer for bark beetle parasites. J. Cheml. Ecol. 7, 359–366 (1981).
Thöming, G., Larsson, M. C., Hansson, B. S. & Anderson, P. Comparison of plant preference hierarchies of male and female moths and the impact of larval rearing hosts. Ecol. 94, 1744–1752 (2013).
Bradley, J. D. Identity of the South-East Asian cocoa moth, Conopomorpha cramerella (Snellen) (Lepidoptera: Gracillariidae), with descriptions of three allied new species. B. Entomol. Res. 76, 41–51 (1986).
Yao, Z. W. & Liu., X. Q. Two gracillariid insect pests attacking Litchi and Longan. Acta. Entomol. Sin. 33, 207–212 (1990).
Ohsaki, N. & Sato, Y. Food plant choice of pieris butterflies as a trade-off between parasitoid avoidance and quality of plants. Ecol. 75, 59–68 (1994).
Ballabeni, P., Wlodarczyk, M. & Rahier, M. Does enemyfree space for eggs contribute to a leaf beetle’s oviposition preference for a nutritionally inferior host plant? Funct. Ecol. 15, 318–324 (2001).
Cunningham, J. P. Can mechanism help explain insect host choice? J. Evolution. Biol. 25, 244–251 (2012).
Gabriella, G. S., Lina, S., Niklas, J. & Sören, N. Host plant choice in the comma butterfly–larval choosiness may ameliorate effects of indiscriminate oviposition. Insect.Sci. 21, 499–506 (2014).
Du, Y. J. & Yan, F. S. The role of plant volatiles in tritrophic interactions among phytophagous insects, their host plants and natural enemies. Acta. Entomol. Sin. 37, 233–250 (1994).
Awmack, C. S. & Leather, S. R. Host plant quality and fecundity in herbivorous insects. Annu. Rev. Entomol. 47, 817–44 (2002).
Knolhoff, L. M. & Heckel, D. G. Behavioral Assays for Studies of Host Plant Choice and Adaptation in Herbivorous Insects. Annu. Rev. Entomol. 59, 263–278 (2014).
Scheirs, J. & De Bruyn, L. Integrating optimal foraging and optimal oviposition theory in plant-insect research. Oikos. 96, 187–191 (2002).
Bruce, T. J., Wadhams, L. J. & Woodcock, C. M. Insect host location: a volatile situation. Trends. Plant. Sci. 10, 269–274 (2005).
Thompson, J. N. Evolutionary ecology of the relationship between oviposition preference and performance of offspring in phytophagous insects. Entomol. Exp. Appl. 47, 3–14 (1988).
Cunningham, J. P., West, S. A. & Zalucki, M. P. Host selection in phytophagous insects: a new explanation for learning in adults. Oikos. 95, 537–543 (2001).
Anderson, P. & Anton, S. Experience-based modulation of behavioural responses to sensory cues in insect herbivores. Plant. Cell. Environ. 37, 1826–1835 (2014).
Jaenike, J. On optimal oviposition behaviour in phytophagous insects. Theor. Popul. Biol. 14, 330–356 (1978).
Mayhew, P. J. Adaptive patterns of host–plant selection by phytophagous insects. Oikos. 79, 417–428 (1997).
West, S. A. & Cunningham, J. P. A general model for host plant selection in phytophagous insects. J. Theor. Biol. 214, 499–513 (2002).
Bruce, T. J. A. & Pickett, J. A. Perception of plant volatile blends by herbivorous insects – Finding the right mix. Phytochemistry. 72, 1605–1611 (2011).
Krieger, J. & Breer, H. Olfactory reception in invertebrates. Science. 286, 720–723 (1999).
Guo, Y. H., Ye, H. J., Fang, W. & Gu, W. X. Study on volatile constituents of 4 cultivars of litchi with differentresistant ability to Conopomorpha sinensis Bradley. Nat. Prod. Res. Dev. 25, 1218–1221 (2013).
Bianchin, J. N. et al. Screening of volatile compounds in honey using a new sampling strategy combining multiple extraction temperatures in a single assay by HS-SPME–GC–MS. Food. Chem. 145, 1061–1065 (2014).
Acknowledgements
This work was financed by The National Natural Science Foundation of China (31301664), Science and Technology Planning Project of Guangdong Province, China (2014A020208079; 2015A020209091); Science and Technology Planning Project of Guangzhou, Guangdong Province, China (201610010002); Guangdong Academy of Sciences Outstanding Young and Scientific Talent Foundation (rcjj2015). GDAS Special Project of Science and Technology Development (2017GDASCX-0107). We sincerely thank Professor Walter S. Leal from UC Davis for providing scientific advices of this paper.
Author information
Authors and Affiliations
Contributions
X.M. and J.J.H. conceived and designed research. X.M., J.Q.D., M.F.G. and O.Y. G.C. conducted the research. X.M., J.J.H. and Y.H.L. performed the experiments, analyzed data and wrote the paper.
Corresponding authors
Ethics declarations
Competing Interests
The authors declare that they have no competing interests.
Additional information
Publisher's note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Meng, X., Hu, J., Li, Y. et al. The preference choices of Conopomorpha sinensis Bradley (Lepidoptera: Gracilariidae) for litchi based on its host surface characteristics and volatiles. Sci Rep 8, 2013 (2018). https://doi.org/10.1038/s41598-018-20383-4
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41598-018-20383-4
This article is cited by
-
Screening for effective odors through which Conopomorpha sinensis Bradley (Lepidoptera: Gracillariidae) locates its host
Chemoecology (2021)
-
Adaptive changes in morph and preference induced by novel host plants mediate host specialization of the cotton–melon aphid
Arthropod-Plant Interactions (2020)
-
Interference mechanism of Sophora alopecuroides L. alkaloids extract on host finding and selection of the Asian citrus psyllid Diaphorina citri Kuwayama (Hemiptera: Psyllidae)
Environmental Science and Pollution Research (2019)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.