New 2-Aryl-9-methyl-β-carbolinium salts as Potential Acetylcholinesterase Inhibitor agents: Synthesis, Bioactivity and Structure–Activity Relationship

Abstract

A series of 2-aryl-9-methyl-β-carbolinium bromides (B) were synthesized and explored for anti-acetylcholinesterase (AChE) activities in vitro, action mechanism and structure-activity relationship. All the compounds B along with their respective 3,4-dihydro intermediates (A) presented anti-AChE activity at 10 μM. Thirteen compounds B showed the excellent activity with IC₅₀ values of 0.11–0.76 μM and high selectivity toward AChE relative to butyrylcholinesterase (BChE), superior to galantamine (IC₅₀ = 0.79 μM), a selective AChE inhibitor drug. Kinetic analysis showed that the action mechanisms of both compounds B and A are a competitive inhibition model. Structure-activity relationship analyses showed that the C = N⁺ moiety is a determinant for the activity. Substituents at 6, 7 or 4′ site, the indole-N-alkyl and the aromatization of the C-ring can significantly improve the activity. Molecular docking studies showed that the compounds could combine with the active site of AChE by the π-π or cation-π action between the carboline ring and the phenyl rings of the residues, and the β-carboline moiety is embedded in a cavity surrounded by four aromatic residues of Trp86, Tyr337, Trp439 and Tyr449. The present results strongly suggest that the para-position of the D-ring should be a preferred modification site for further structural optimization design. Thus, 2-aryl-9-methyl-β-carboliniums emerged as novel and promising tool compounds for the development of new AChE inhibitor agents.

Introduction

As the most common form of adult onset dementia, Alzheimer’s disease (AD) is an age-related irreversible neurodegenerative disorder characterized by a progressive memory loss, a decline in language skills and other cognitive impairments¹. It was estimated that 47 million people suffered from dementia worldwide in 2016, and this number is predicted to increase to more than 131 million by 2050. The total estimated worldwide cost of dementia is US$818 billion, and it will become a trillion dollar disease by 2030². Therefore, given the aging of the population, AD is becoming one of the main public health issues we have to face.

AD is a complicated disease involving different molecular events. Its pathological features include tau-protein aggregation in nerve cells, β-amyloid protein aggregation in the spaces between nerve cells, oxidative stress and lowered levels of acetylcholine in the brain³. Among them, cholinergic hypothesis is the most popular explanation of mechanism of AD development⁴. Neuropathological evidence has proved that the memory impairment and behavioral abnormalities in patients with AD results from low level of acetylcholine (ACh) in different areas of the central nervous system, mainly the cerebral cortex and the hippocampus. Therefore, AChE inhibitors has been the leading strategy for the development of AD drugs, which can elevate the level of acetylcholine in the synapses between cholinergic neurons and enhance cholinergic function⁵. In addition, AChE inhibitors are also used for the treatment of senile dementia, ataxia, myasthenia gravis and Parkinson’s disease⁶, or as insecticides in modern agricultural procedures⁷.

Acetylcholinesterase and butyrylcholinesterase (BuChE) are two different types of cholinesterases present in the human brain, which catalyze the hydrolysis of choline-based esters to terminate cholinergic signal transmission. The two enzymes are distinguished on the basis of substrate specificities, tissue distribution and sensitivity to inhibitors^8,9. AChE mainly exist in the synapses of the central and peripheral nervous systems and in the membranes of erythrocytes, while BuChE is primarily present in the blood and glial cells or neurons^10,11. AChE hydrolyzes acetylcholine (ACh) more quickly whereas BuChE hydrolyzes butyrylcholine (BuCh) more quickly. Under normal conditions, acetylcholine (ACh) is dominantly decomposed by AChE instead of BuChE although both AChE and BuChE can hydrolyze ACh^12,13. Therefore, AChE has been considered the main targeting cholinesterase in the AD scenario. Current clinical therapeutic strategy for mild-to-moderate AD is mainly to improve cholinergic neurotransmission by using AChE inhibitors¹⁴. However, it is worth mentioning that BuChE was also found to have a critical role for ACh hydrolysis in AD, especially in the late stage of AD^15,16. In progressed AD, level of AChE in brain declines while BuChE increases, leading to ratio of BuChE/AChE shifting from 0.6 to 1.1^17,18. At this point, BuChE can compensate for AChE loss by hydrolyzing ACh in cholinergic transmission. Therefore, to avoid the adverse effects caused by suppression of AChE, exploits of BuChE inhibitors for AD treatment have also aroused a worldwide popularity. A lot of effective and selective BuChE inhibitors have been discovered¹⁹. Furthermore, it was found that the two enzymes are also related with the formation of amyloid protein plaques, which is encouraging the development of dual- or multi-functional ChE inhibitors²⁰.

At present, there are four FDA-approved AChE inhibitor-type drugs (tacrine, donepezil, galantamine and rivastigmine) for treatment of cognitive dysfunction and memory loss associated with mild-to-moderate AD²¹. Among them, donepezil and galantamine are reversible and selective AChE inhibitors that compete with acetylcholine for AChE binding²². Recent researches showed that AChE inhibitors not only alleviate the cognitive defect of AD patients by elevating acetylcholine (ACh) levels, but also act as disease modifying agents by preventing the early step of AD, the assembly of β-amyloid peptide (Aβ) into amyloid plaque^23,24. Nevertheless, some obvious adverse effects including nausea and vomiting, decreased appetite, weight loss, hepatotoxicity or problems associated with bioavailability were also reported for these drugs^25,26,27,28. Additionally, these drugs are only focused on the symptomatic aspects but cannot prevent, halt or reverse the progression of the disease¹⁴. Therefore, the search of central selective AChEIs devoid of adverse effects is still a challenging research topic. It is very necessary to find better AChE inhibitor agents and effective therapeutics for AD desease²⁹.

To date, a lot of highly active AChE and/or BuChE inhibitors had been found, including synthetic, semi-synthetic and natural compounds³⁰. It is worth noting that most of the compounds are nitrogen-containing compounds or alkaloids. Among them, β-carboline (pyrido[3,4-b]indole) compounds are one important type. β-Carboline alkaloids, also referred to as harman alkaloids, were widely distributed in organisms including plants, animals, halobios and human being³¹. In addition, β-carbolines occur in foods and cigarette smoke, suggesting human uptake and exposure to these compounds³². In the past decades, β-carbolines have attracted lots of attention from researchers due to their diverse biological activities such as antimicrobial³³, antitumor³⁴, antiviral³⁵ and antiparasitic³⁶, anti-inflammatory³⁷, vasorelaxant³⁸, antioxidant³⁹, neuroactive or neurotoxic actions⁴⁰. Especially interesting for us is that some natural or synthetic quaternary 2-methyl-β-carboline salts were also found to have strong AChE inhibitory activity^41,42. The results suggest that β-carboline is a promising molecular framework for development of new anti-AChE agents.

With the aim of finding more potent β-carboline-type AChE inhibitors for treatment of AD, herein, a series of new β-carboline derivatives (Fig. 1) were designed and explored for AChE inhibition activity, action mechanism as well as structure-activity relationship (SAR). These compounds are characterized by a quaternary 2-phenyl-9-methyl-β-carbolinium skeleton with various substituents on the 2-aryl ring.

Figure 1

Synthetic route of target compounds B1-B34 and C1. Reagents and conditions: (a) phenylhydrazine-HCl, H₂O, 12 h at r.t.; (b) EtOH, con. H₂SO₄, reflux for 12 h; (c) R²I or R²Br, NaH, dry DMF, 0 °C; (d) LiAlH₄, dry THF, 0 °C to r.t.; (e) active MnO₂, CHCl₃, r.t.; (f) MsCl, Et₃N, LiBr, dry THF, 0 °C to r.t.; (g) R-PhNH₂, TsOH·H₂O, EtOH, r.t.; (h) Pd/C, acetonitrile, reflux; (i) NaBH₄, EtOH, r.t.

Results and Discussion

Chemistry

Based on the consideration of SAR and structural diversity, we designed a series of 2-aryl-9-mehtyl-β-carboline salts (B1-B33) with various substituents on the D-ring (Fig. 1) in order to get an insight into the effect of the substitution pattern of the D ring on anti-AChE activity. The substituents include both electron-withdrawing groups like halogen atom, CN, CF₃, NO₂, and electron-donating groups like CH₃, OCH₃ and OH. The substitution position involves the 2′, 3′ or 4′ site of the D-ring. In addition, we also designed one the indole-N-H instead of N-Me derivative (B34) and one 1,2,3,4-tetrahydro derivative (C1) of compounds B in order to explore the effect of the indole-N-substituents and the C-ring aromatization on the activity. The substitution patterns of target compounds are depicted in Table 1.

Table 1 Structures and inhibitory activity of compounds A and intermediates B against AChE. ^aThe test concentration of the compound is 10 μM. The differences between data with the different lowercases within a column are significant (p < 0.05).

The synthetic route of target compounds is shown in Fig. 1, in which phenylhydrazine–HCl and α-ketoglutaric acid were used as the starting materials. According to our reported method³³, key intermediates A1-A35 were synthesized via 6–7 steps. In sequence, the intermediates A1-A34 were dehydrogenated by Pd/C in acetonitrile or toluene to provide the final compounds B1-B34. Compound A1 was reduced with NaBH₄ to obtain its 1,2,3,4-tetrahydro derivative C1.

Compounds B include 32 new compounds (B2-B33) and 2 known compounds (B1, B34). The known compounds B1, B34 and intermediates A1-A35 were confirmed by comparison of NMR and MS data and those reported in literature³³. New compounds B2-B33 were elucidated by ¹H NMR, ¹³C NMR and HRMS analyses. All compounds B showed some similar spectroscopic characteristics because of the structural similarity. Each compound showed a characteristic ion peak at m/z [M-Br]⁺ in positive ESI-HRMS spectra. The presence of bromide anion was confirmed by ion peaks at m/z 79 and 81 in negative ESI-MS spectra. In ¹H and ¹³C NMR spectra, each compound B revealed one signal of H-1 at δ_H ca. 10.0 (1 H, s or d, J = ca 1.2 Hz) and one signal of C-1 in the range of δ_C 152–163, one doublet signal of H-4 at δ_H ca. 9.02 (1 H, d, J = ca 6.5 Hz), one doublet or double doublet signal of H-3 at δ_H ca. 9.00 (1 H, d, J = ca. 6.5 Hz, or dd, J = ca. 6.5, ca. 1.2 Hz), one signal of C-4 at δ_C ca. 130 and one signal of C-3 at δ_C ca.133. The NMR spectra of C1 were similar to that of B22 except for three additional CH₂ signals at δ_H 4.38 (2 H, s, H-1)/δ_C 46.6 (C-1), 3.60 (2 H, t, J = 5.6 Hz, H-3)/δ_C 48.7 (C-3), 2.93 (2 H, t, J = 5.6 Hz, H-4)/δ_C 21.8 (C-4) and fewer signals of one HC=N⁺ unit and one AX system [C(3)H=C(4)H].

AChE inhibition activity

According to Ellman’s coupled enzyme assay⁴³, compounds B1-B34, C1 along with intermediates A1-A35 were screened for inhibition activity on AChE at 10 μM. Galantamine, a selective and competitive AChE inhibitor drug for treatment of AD, was used as a reference control. The results are shown in Table 1.

The data in Table 1 showed that all compounds B and the majority of intermediates A presented some anti-AChE activity at 10 μM. However, compared with compounds A, except for B14, all compounds B showed the much higher activity. Among compounds B, thirty-two compounds displayed inhibition rates of >50%, and nine compounds (B7, B10, B13, B19, B22, B27, B30, B32 and B33) showed the inhibition rates of 90.6% to 96.7%, higher or equal to galantamine (91.2%). B33 (R = 2′,4′-diBr, R′ = Me) showed the highest activity. In contrast to compounds B, only the minority of compounds A (A1, A4, A7, A10, A13, A15, A16, A19, A22, A34-A43 and A46) showed the moderate inhibition rates of 40% to 78%.

In order to explore anti-AChE potential more detail, the compounds with the higher initial activity in Table 1 were further determined for median inhibition concentrations (IC₅₀) on AChE. Galantamine was used as a reference standard. The results are listed in Table 2. Gratifyingly, thirteen compounds B (B7, B10, B13, B19, B22, B27, B30, B32, B33, B35, B37, B38 and B40) showed the excellent activity with IC₅₀ values of ≤0.76 μM, higher or approximately equal to that of galantamine (IC₅₀ = 0.79 μM), agreement with that showed in Table 1. Among them, B33 (IC₅₀ = 0.11 μM) was most active, superior to rivastigmine (IC₅₀ = 9.94 μM)⁴⁴ and tacrine (IC₅₀ = 0.25 μM)⁴⁵ but inferior to donepezil (IC₅₀ = 0.03 μM)⁴⁶. In contrast to compounds B, all the compounds A in Table 2 showed the low to the moderate activity (IC₅₀ values > 1.4 μM).

Table 2 Median inhibition concentrations of part of compounds B and intermediates A against AChE and BuChE. ^aSI: selectivity index, the ratio value of IC₅₀ (BuChE)/IC₅₀ (AChE); ^bEstimated values based on the results in Table 1; ^cND: no determination. ^dNA: no inhibition activity at 10 μM; ^eThe data are cited from ref.⁴⁴; ^fThe data are cited from ref.⁴⁵. ^gThe data are cited from ref.⁴⁶.

There is increasing recognition that BuChE may play an important role in cholinergic transmission^15,16. While the level of AChE decreases dramatically in the advanced stage of AD, BuChE increases in brain regions involved in cognitive functions^17,18. At this point, BuChE is able to compensate for AChE loss by hydrolyzing ACh. These observations lead one to believe that inhibition of both AChE and BuChE may contribute to improvement of current symptomatic treatments of AD. Therefore, the compounds in Table 2 were also evaluated for BuChE inhibition activity by means of Ellman’s method⁴³. Galantamine was used as a reference standard. The results in Table 2 showed that the compounds also exhibited some inhibition activity on BuChE with IC₅₀ values of 3.90 to 212.2 μM, but compared with the anti-AChE activity of each the compound, its anti-BuChE activity is obviously lower except for A1 and A34. The results above showed that both the compounds B and A had the higher selectivity for AChE than BuChE. Compared with galantamine, thirteen compounds B (B7, B10, B13, B19, B22, B27, B30, B32, B33, B34, B35, B38 and B40) also showed the higher selectivity for AChE in addition to the higher anti-AChE activity. Among them, the selectivity indexes (SI) of compounds B30, B32 and B33 reached up to >240, comparable with that of donepezil (SI = 180)⁴⁶, a selective AChE inhibitor drug. Relative to compounds B, compounds A in Table 2 showed the lower selectivity to AChE. Unexpectedly, compounds A1 and A46 (SI < 1.0) showed the higher selectivity to BuChE but not AChE.

As the representative compounds, compound B33 with the highest anti-AChE activity among compounds B and compound A22 with the highest anti-AChE activity among compounds A were further evaluated for cytotoxic activities on both mouse neuroblastoma N2a cells and primary cultured porcine fetal kidney cells. Meanwhile, in order to compare the cytotoxicity of compounds A and B, compound B22 was subjected to the assay. The results in Table 3 showed that both B33 and B22 showed the much lower cytotoxicity with IC₅₀ values of 10.6 and 19.2 μM on mouse neuroblastoma N2a cells compared with their respective anti-AChE activity (IC₅₀ = 0.11, 0.29 μM). Similarly, both B33 and B22 also showed the much lower cytotoxicity on primary cultured porcine fetal kidney cells (IC₅₀ = >20, 18.1 μM). Compared with B22, compound A22 showed the higher cytotoxicity with IC₅₀ values of 2.13 and 5.18 μM on the two strains of cells although its cytotoxicities are also lower than its anti-AChE activity (IC₅₀ = 1.45 μM).

Table 3 Cytotoxicity of the compounds on mouse neuroblastoma N2a cells and primary cultured porcine fetal kidney cells (48 h). ^a95% CI: the confidence interval of IC₅₀ at 95% probability.

Mechanism of AChE inhibition

In order to know the mechanism of AChE inhibition, compounds B22 and A22 as two representative compounds were conducted for kinetic analysis of AChE inhibition. The graphical analysis of the inhibition data for B22 and A22 in comparison with galantamine is shown in Fig. 2. The results in Fig. 2A and C clearly showed that both B22 and A22 inhibited AChE in a competitive manner with the substrate acetylthiocholine iodide (ATCh). The estimates of the inhibition constants K_i of B22 and A22 for AChE were 4.25 × 10⁻⁸ M and 4.83 × 10⁻⁷ M, respectively, while the K_m value of acetylthiocholine iodide (ATCh) for AChE was 1.63 × 10⁻⁴ M. The results showed that the binding capacity of B22 with AChE is approximately 11-fold that of A22 and 3835-fold that of the substrate ATCh.

Figure 2

The Mechanism of AChE inhibition by compound B22 (A) and intermediate A22 (C) respective to ATCh, and their K_i determination (B,D). (A and B) The reciprocals of the initial reaction rates and substrate concentrations are plotted. (C and D) The slope values of the lines from graph A or C are plotted versus the inhibitor concentration, affording an equation of linear regression. When y is 0, the equations give K_i values of 4.25 × 10⁻⁸ M for compound B22 and 4.83 × 10⁻⁷ M for compound A22.

Structure-activity relationships

By comparison of the activity and structures of the compounds in Tables 1 and 2, we can find some clear and regular structure-activity relationships for compounds B and A (Tables 4, 5 and 6).

Table 4 Effect of substitution patterns of the D-ring on the activity of compounds B and A. ^aArrows outside of parentheses represent compounds B; arrows inside parentheses represent compounds A. ^b↑significantly increasing the activity relative to R = H; ↓significantly decreasing the activity; ±, slight change of the activity. ^c“nd” means no determination.

Table 5 Effect of substitution patterns of the D-ring on the activity of compounds B and A. ^a↑significantly increasing the activity relative to R = H; ↓significantly decreasing the activity; ±, slight change of the activity. ^b“nd” means no determination.

Table 6 Effect of the indole-N-alkyl and substitution patterns of the A-ring on the activity of compounds B and A. ^a↑, significantly increasing the activity relative to R = H; ↓, significantly decreasing the activity; ±, slight change of the activity. ^b“nd” means no determination.

Firstly, the substitution pattern of the D-ring can dramatically influence the activity. For compounds B, the p-substitution of all the substituents except cyano group (B25, A25) can significantly increase the anti-AChE activity (B4, B7, B10, B13, B16, B19, B22, B27 vs B1) whereas the o- or m-substitution generally leads to decrease or slight change of the activity. A similar trend was also found in compounds A (A4, A7, A10, A13, A16, A19, A22 vs A1). The substituents above not only include electron-donating groups like OH, OMe or Me and electron-withdrawing groups like halogen atoms or CF₃ but also involve hydrogen-bond acceptors (OMe) and hydrogen-bond donors (OH). Therefore, we speculated that the effect of the p-substituents on the activity should be mainly steric effect but not electron effect or hydrogen-bond effect. For p-mono-substituted compounds B, the order of the anti-AChE activity is the iodide (B13) ≈ the Me-substituted compound (B22) > the bromide (B10) ≈ the MeO-substituted compound (B19) > the chloride (B7) > the CF₃-substituted compound (B27) > the fluoride (B4) ≈ the OH-substituted compound (B16). The results above strongly suggest that the 4′ site of the D-ring should be one important modifiable position for further structure optimization.

For BuChE, the effect of substituents on the D-ring on the activity strongly depends on the structure of the C-ring. For compounds B with the aromatic C-ring, the introduction of substituents to the D-ring causes increase of the anti-BuChE activity in most cases. However, the opposite was observed for compounds A with a nonaromatic C-ring. Nevertheless, 4′-substituents (Cl, Br, I, Me, OMe, OH, CF₃) can significantly increase the selectivity of both compounds B and A to AChE at the same time.

For the dihalides (B29‒33), the presence of 2′,4′-dihalogen atoms (B30, 32, 33) can significantly improve both the anti-AChE activity and selectivity, whereas the presence of 2′,6′-difluoro or 3′,5′-dichloro leads to dramatic decrease of the activity (B29, B31 vs B1). Compared the corresponding 4′-mono-halogenated compounds, 2′,4′-dihalogenated compounds showed the slight improvement of the anti-AChE activity although they exhibited the much higher selectivity for AChE (B30 vs B7; B32 or B33 vs B10). The results above show that for the 2′,4′-halogenated compounds, 2′-halogen atom can significantly increase the selectivity to AChE but not greatly influences the anti-AChE activity (B30 vs B7; B32 or B33 vs B10). In other words, there may be a synergistic effect on the anti-AChE selectivity between 2′-Cl and 4′-Cl, 2′-F and 4′-Br or 2′-Br and 4′-Br. The selectivity indexes for AChE of the 2′,4′-dibromo compound (B33), the 2′-fluoro-4′-bromo compound (B32), 2′,4′-dichloro compound (B30) reach up to 278, 385 and 241, respectively.

As strong electron-withdrawing substituents, cyano, nitro or trifluoromethyl group on the D-ring generally causes decrease of the activity of compounds B (B24-26, B28 vs B1). The exception is that 4′-CF₃ is able to dramatically improve the activity (B27 vs B1). Unlike compounds B, the above substituents or double halogen atoms on the D-ring always leads to decrease of the activity of compounds A (A22-33 vs A1).

Secondly, the C=N⁺ moiety of the compounds should be a determinant for the activity because transformation of the 3,4-dihydro-β-cabolinium (A22) or aromatic β-cabolinium (B22) to its 1,2,3,4-tetrahydro-derivative C1 leads to complete loss of the activity. A similar case was also observed in some tertiary aromatic β-carbolines and their 1,2,3,4-tetrahydro-β-carbolines, where only quaternary aromatic 2-methyl-β-carboliniums showed the higher activity, whereas its corresponding tertiary aromatic β-carboline derivatives without 2-methyl, 2-methyl-1,2,3,4-tetrahydro-β-carbolines or quaternary 2,2-dimethyl-1,2,3,4-tetrahydro-β-carboline salts only exhibited very weak or no activity^41,42.

Thirdly, compared with B46 (the indole-N-H), the indole-N-alkylation can significantly improve the anti-AChE activity of compounds B. This effect varies with the type and steric hindrance of the alkyl groups. A similar case was also observed for compounds A. Among all the substituents, iso-propyl (B40) and ethyl (B38) can give the best improvement of the anti-AChE activity followed by iso-butyl (B43) and allyl (B41) while benzyl has the weakest effect on the activity. Methyl, propyl or butyl cause the moderate effect. The only exception is that —CH₂CO₂Et has almost no effect on the activity (B45 vs B46, Table 1). On the contrary, for anti-BuChE activity, whether for compounds B or A, the indole-N-alkylation always causes decrease of the activity compared with B46 or A46 (the indole-N-H). The above SARs are obviously different from that of 2-methyl-β-carbolinium salts, where the indole-N-methylation is not able to significantly increase the anti-AChE activity⁴¹, even decrease the activity⁴². The results above suggest that the effect of the indole-N-alkylation on the activity of the aromatic 2-substituted-β-carboliniums is also related with the type of the pyridine-N-substituents.

It is worth noting that for compounds A, the effect of the indole-N-alkylation on the activity varies with the substituents on the D-ring. When the D-ring is phenyl (R=H), the indole-N-methylation hardly impact the activity (A1 vs A46). However, when the D-ring is 4′-bromrophenyl, the indole-N-methylation causes the significant increase of the activity (A10 vs A47, Table 1). We assume that a similar case might also exist in compounds B.

Fourthly, comparison of B34-B37 and B1 or A34-A37 and A1 suggests that substitution patterns of the A-ring can also dramatically influence the activity. The introduction of 6-OMe, 6-Me, 7-F or 7-Cl to the A-ring leads to obvious increase of the anti-AChE activity of both compounds B and A. However, different cases exist in aspect of anti-BuChE activity. The substituents on the A-ring can also improve the anti-BuChE activity of compounds B (B37-B37 vs B1) but decrease that of compounds A (A37-A37 vs A1). Since the substituents above involve different electron effect, we speculated that the effect of substituents on the A-ring might mainly be steric effect but not electron effect.

Finally, besides the substituents on the A- and D-ring and the indole-N-substituents, the aromatization of the C-ring can also intensely influence the anti-AChE activity. Among these factors, there is an interaction effect on the activity. The aromatization of the C-ring dramatically enhances the activity of the indole-N-alkyl compounds (B1‒B45 vs A1‒A45), but hardly influences the activity of the indole-N-H compounds (A45 vs B45), indicating that the influence of the aromatization of the C-ring on the activity is related with the indole-N-substituents. Compound A33 was no activity at 10 μM whereas B33 showed the highest activity; compound A7 was more active than compound A4, but B7 was less active than B4. This result shows that the influence of the aromatization of the C-ring on the activity also closely varies with the substituents on the D-ring. Furthermore, the presence of 4′-Br can improve the activity of the indole-N-Me compounds (A10 vs A1, B10 vs B1) but decrease the activity of the indole-N-H compounds (A47 vs A46, Table 1), suggesting that an interaction effect on the activity also exists between the substituents on the D-ring and the indole-N-substituents.

For BuChE, a similar interaction effect on the activity also exists among the four factors. For example, for the N₉-Me compounds, the C-ring aromatization improves that activity of the 4′-F, 4′-Br, 4′-I, 4′-OMe or 4′-Me compounds (B4 vs A4, B10 vs A10, B13 vs A13, B19 vs A19, B22 vs A22) but decreases the activity of the 4′-Cl, 3′-OH or 4′-OH compounds (B7 vs A7, B15 vs A15, B16 vs A16). For the indole-N-H compound, the C-ring aromatization also causes decrease of the activity (B46 vs A46). Interestingly, whether for the indole-N-alkyl compounds or for the indole-N-H compounds, the C-ring aromatization always leads to increase of the selectivity toward AChE (B1 vs A1; B4 vs A4; B7 vs A7; B10 vs A10; B15 vs A15; B16 vs A16; B19 vs A19; B22 vs A22; B34-45 vs A34-45; B46 vs A46) (Table 2).

Molecular docking of compounds with AChE and BuChE

To gain insight into binding interactions of the compounds in the hydrolytic active site of AChE and BuChE, molecular docking studies were performed for the representative compounds B15, B16, B18, B19, B21, B22 and A22. These studies were performed into an AChE structure (PDB 4BDT) and BuChE (PDB 5K5E). The results are shown in Figure 3 and Table 7. Figure 3A revealed that the binding modes of huprine W and 6QS obtained by AutodDock are almost identical to the crystallographic structure, which proves the feasibility of the docking protocol. The data in Table 5 show that the binding free energies (<−9.3 kcal/mol) of compounds A or B with the catalytic site of AChE are larger than that (−7.2 to −8.3 kcal/mol) with BuChE, indicating that compounds A or B have more inhibition potential to AChE than BuChE, agreement with the measured inhibition activities. The results of molecular docking showed that the compounds have very similar binding modes (Fig. 1B–E and F). Figure 3B–E showed that the compounds could be stabilized in the active site of AChE by interactions similar to those described for huprine W. The β-carboline moiety is embedded in a remarkable group of aromatic rings including Trp86, Tyr337, Trp439 and Tyr449. The B-ring of the β-carboline is facing Tyr337 while the C-ring is facing Trp86 (Fig. 3B). Obviously, the full aromatic β-carboline moiety should be more beneficial than its 3,4-dihydro structure for enhancing the binding affinity of the ligands for AChE due to the π-π action between the carboline ring and the indole ring of the residue Trp86. The speculation was further supported by the compounds B having larger binding free energy with the catalytic site of AChE than compounds A (Table 7, B13 vs A13, B15 vs A15, B19 vs A19, B22 vs A22).

Figure 3

The estimated binding modes of the compounds into the active site of AChE and BuChE. The protein structures are show in Ribbon style with coil in green, helix in red and strand in yellow. The binding site residues are displayed using a stick model with carbon in green. The potential hydrogen contacts between compounds and protein were highlighted by yellow dashed lines. (A) Superposition of huprine W in the X-ray crystallographic structure (PDB code 4BDT; orange) and in docking resultant complex structure of the AChE (dark green). (B–E) The binding modes of the compounds (in stick model with carbon) into the active site of AChE: (B) B22 (cyan) and A22 (pink); (C) B22 (cyan) and B21 (goldenrod); (D) B19 (chartreuse) and B18 (medium blue); (E) B16 (purple) and B15 (orange red). (F) The binding modes of compounds B22 (cyan) and A22 (pink) into the active site of BuChE. Other atoms were colored as follows: nitrogen, blue; oxygen, red; hydrogen, gray.

Table 7 The binding free energies of compounds with AChE and BuChE. ^aThe inhibitor co-crystals with AChE in the crystal structure of the protein complex (PDB code: 4BDT). ^bThe inhibitor co-crystals with BuChE in the crystal structure of the protein complex (PDB code: 5K5E).

The binding models of B22/21 (Fig. 3C) or B19/18 (Fig. 3D) showed that the 3′-substituents may bring some inappropriate clash penetration by the ligand into the enzyme, whereas 4′-substituents generally increase the binding free energy of AChE-the compound. Similar situations arise in other compounds, such as B16/B15 (Fig. 3E), B13/B12, A16/A15, et al. Interestingly, Fig. 3E showed that the hydrogen atom of 3′-OH group and the phenolic oxygen atom of Tyr124 could form an H-bond (Fig. 3E), which compensates the inappropriate clash penetration, resulting in the comparable binding free energy for A16 vs A15 or B16 vs B15. These results support our presumption above that the effect of the p-substituents on the activity should be mainly steric effect but not electron effect or hydrogen-bond effect.

All of the current marketed AChEIs are nitrogen-contained compounds, which are partly protonated at physiological pH. In the acid-base equilibrium, the cations formed by protonation can enhance the overall affinity of AChE for the ligands by additional cation–π interactions with aromatic residues at the active site of AChE, whereas their neutral can cross the blood‒brain barrier (BBB) due to their good lipophilicity⁴⁷. In the present study, ionic compounds A22 and B22 showed the higher activity against AChE, but their neutral derivative C1 lost the activity (Table 2). Obviously, the positive charge of compounds A and B should be a crucial factor for the activity. Molecular docking showed that the β-carboline moiety locates in the cavity consisting of four aromatic residues of Trp-86, Trp-439, Tyr-337 and Tyr-449 at the active site of AChE (Fig. 3B–E). All the aromatic rings of these residues are rich in π-electrons. Therefore, the positively charged β-carboline moiety with the π-electron poor property can form stronger π-π action with the residues to improve the overall affinity of AChE for the compounds or the activity of the compounds.

Similarly, the low activity of the indole-N-H compounds may also be explained by loss of the positive charge. Theoretically, the indole-N-H has stronger acidity because of the presence of the C=N⁺ moiety adjacent to the N₉ site. Therefore, the indole-N-H compounds such as B34 and A35 can partly release their protons in aqueous solution to form inactive or low active neutral forms (Fig. 4). In other word, the indole-N-H compounds can co-exist in two forms of ion and non-ion in aqueous solution, but only their ionic forms have the good activity. Unlike the indole-N-H compounds, the indole-N-Me compounds always exist in ionic forms. This may be why the indole-N-methylation can increase the activity of compounds B (B1 vs B34, Table 2) or A (A10 vs A35, Table 1). Additionally, the similar case was not observed for 2-methyl-β-carbolinium salts^41,42, where their indole-N-substiutents did not significantly influent the activity against AChE. The reason is that the non-ionic form formed by release of the indole-N-H of 2-methyl-β-carbolinium salts is unstable because of the lack of conjugation action of the D-aryl ring.

Figure 4

Possible existing forms of N₉-H-type compounds in an aqueous solution.

Sanguinarine and chelerythrine are two quaternary benzo[c]phenanthridine alkaloids, which contain a C=N⁺ moiety with high chemical activity. Studies showed that the two can react with OH⁻ or H₂O to yield the corresponding pseudobase or dimer by its C=N⁺ moiety, and mainly exist in its dimer in pH 7.0 aqueous solution^48,49. Therefore, both sanguinarine and chelerythrine can easily cross the membrane of cells to quickly enter living cells although they are ionic compounds⁵⁰. Additionally, we also found the similar chemical transformation for 2-aryl-3,4-dihydroisoquinoliniums containing a C=N⁺ moiety (unpublished). Based on the results above and the structural similarity, we speculated that compounds A or B could also co-exist in two forms of ion and their corresponding pseudobases or dimers in human blood (pH 7.35–7.45) due to the presence of a C=N⁺ moiety with high chemical activity (Fig. 5). The resulting pseudobase or dimer forms can allow compounds A or B to cross the blood brain barrier (BBB) due to its good lipophilicity. At present, the investigation on BBB permeability of the compounds is ongoing in our lab.

Figure 5

Plausible existing forms of compounds A or B in an aqueous solution.

Besides higher anti-AChE activity and higher selectivity to AChE, compound B22 also showed the lower cytotoxicity than A22, which should be attributed to the aromatization of the C-ring. A similar case was observed for the antifungal activity of compounds A, where the aromatization of the C-ring causes reduction of the activity³³. This similarity might be related with both cytotoxicity and antifungal activity involving inhibition of cell proliferation. Obviously, for potent and high selective β-carboline-type AChE inhibitors for treatment of AD, the full aromatic 9-alkyl-β-carboline skeleton is crucial whereas for anticancer or antimicrobial, the 9-alkyl-3,4-dihydro-β-carboline skeleton is favorable.

In conclusion, a series of new 2-aryl-9-methyl-β-carboline bromides (B) were synthesized in the present study. All compounds B along with most of their 3,4-dihydro intermediates (A) showed anti-AChE activities in vitro at 10 μM. Among them, nine compounds B showed higher anti-AChE activity and selectivity relative to BChE than galantamine, a selective AChE inhibitor drug, which showed great potential to be developed as new selective AChE inhibitor agents. Kinetic analysis proved that both compounds B and A are able to inhibit AChE in a competitive manner, and the binding capacity of B22 with AChE reaches up to 3835-fold that of substrate acetylthiocholine iodide (ATCh). In aspect of SAR, the C=N⁺ moiety was proved to be a determinant for the activity, and 4′-substituents of the D-ring, the indole-N-methyl and C-ring aromatization can dramatically increase the anti-AChE activity and selectivity by independent or synergistic effect. These findings will be of great importance for further structural optimization design. Thus, we succeeded in discovering a class of new and promising tool compounds for development of new AChE inhibitor agents.

Methods

Chemicals

Acetylthiocholine iodide (ATCh), butyrylthiocholine iodide (BuTCh), 5,5′-dithio-bis(2-nitrobenzoic acid) (DTNB), and galantamine were purchased from Sigma Chemicals Co., Ltd. (Shanghai, China). Phenylhydrazine-HCl and α-ketoglutaric acid were purchased from Aladdin Industrial Inc. (Shanghai, China). Other reagents and solvents were obtained locally and of analytical grade or purified according to standard methods before use. The water used was redistilled and ion-free.

Enzymes

AChE (E.C. 3.1.1.7) (BR, 200 u/mg) from fly’s head and BuChE (E.C. 3.1.1.8) (BR, 20 u/mg) from horse serum were purchased from Shanghai Yuanye biological technology Co. Ltd (Shanghai, China).

Instruments

Melting points were determined on a digital melting point apparatus. Nuclear magnetic resonance spectra (NMR) were performed on an Avance III 500 MHz instrument (Bruker, Karlsruhe, Germany). Chemical shifts (δ values) and coupling constants (J values) are given in parts per million and hertz, respectively. High-resolution mass spectra (HR-MS) were carried out with a microTOFQ II instrument (Bruker). Low-resolution mass spectra were carried out with a LCQ Fleet instrument (Thermo Fisher, Shanghai, China).

Synthesis

General procedure for the synthesis of compounds A1-A47

According to our previous method³³, intermediates A1-A47 were prepared via 5 or 6 steps starting from phenylhydrazine-HCl and α-ketoglutaric acid. The compounds were confirmed by ¹H NMR, ¹³C NMR and MS analyses, and their spectral data were agreement with those in literature²³.

General procedure for the synthesis of compounds B1-B46

To a solution of compounds A (0.2 mmol) in acetonitrile (40 mL) was added 5% Pd/C (wetted with ca. 55% water) (0.17 mmol, 80 mg). The mixture was refluxed at 80 °C for about 3 days to complete the reaction. The Pd/C powders in the reaction solution was filtered off through a sand core funnel and completely washed with methanol. The combined solution was evaporated under vacuum to yield the desired compounds.

9-Methyl-2-phenyl-β-carboline bromide (B1): Yield 78%; yellow powders; mp 271.2–272.8 °C; The ¹H NMR, ¹³C NMR and MS data are consistent with those in the literature³³.

9-Methyl-2-(2-fluorophenyl)-β-carboline bromide (B2): Yield, 29%; golden yellow powders; mp 247.6–249.0 °C; ¹H NMR (500 MHz, DMSO-d₆) δ: 9.99 (1 H, s, H-1), 9.07 (1 H, d, J = 6.5 Hz, H-4), 9.00 (1 H, d-like, J = 6.5 Hz, H-3), 8.67 (1 H, d, J = 8.0 Hz, H-5), 8.04 (1 H, 2 × t, J = 7.9, 1.7 Hz, H-6′), 8.01–7.95 (2 H, m, H-7, H-8), 7.84 (1 H, m, H-4′), 7.75 (1 H, t-like, J = 9.4, 8.5, 1.3 Hz, H-3′), 7.63 (1 H, t, J = 7.7 Hz, H-5′), 7.58 (1 H, 2 × t, J = 7.9, 1.5 Hz, H-6), 4.17 (3 H, s, NCH₃); ¹³C NMR (125 MHz, DMSO-d₆) δ 155.4 (d, J = 252.1 Hz, C-2′), 146.0 (C-1), 136.4 (C-8a), 134.5 (C-8a), 133.7 (d, J = 7.9 Hz, C-4′), 133.5 (C-3), 133.1 (C-9a), 131.5 (d, J = 11.7 Hz, C-1′), 131.0 (C-4b), 128.9 (C-6′), 126.3 (d, J = 3.8 Hz, C-5′), 124.7 (C-7), 122.7 (C-5), 119.4 (C-6), 118.1 (C-8), 117.7 (d, J = 18.8 Hz, C-3′), 112.0 (C-4a), 31.2 (NCH₃); HR-ESI-MS [M-Br]⁺: Calcd for C₁₈H₁₄FN₂⁺, 277.1136, found 277.1170.

9-Methyl-2-(3-fluorophenyl)-β-carboline bromide (B3): Yield, 50%; orangered powders; mp 143.0–144.0 °C; ¹H NMR (500 MHz, DMSO-d₆) δ 9.97 (1 H, d, J = 1.1 Hz, H-1), 9.04 (2 H, s, H-3, H-4), 8.67 (1 H, d, J = 8.0 Hz, H-5), 8.06 (1 H, 2 × t, J = 9.5, 2.3 Hz, H-2′), 8.01–7.93 (2 H, m, H-7, H-8), 7.92–7.82 (2 H, m, H-5′, H-6′), 7.66 (1 H, t-like, J = 8.4 Hz, H-4′), 7.57 (1 H, 2 × t, J = 8.0, 1.4 Hz, H-6), 4.20 (3 H, s, NCH₃); ¹³C NMR (126 MHz, DMSO-d₆) δ 162.5 (d, J = 247.1 Hz, H-3′), 145.9 (C-1), 145.0 (d, J = 10.3 Hz, H-1′), 136.4 (C-8a), 133.32 (C-3), 133.26 (C-4b), 132.9 (C-9a), 132.5 (d, J = 8.8 Hz, C-5′), 129.8 (C-4), 124.7 (C-7), 122.7 (C-5), 122.1 (d, J = 3.2 Hz, C-6′), 119.4 (C-6), 118.23 (d, J = 21.1 Hz, C-2′), 118.19 (C-8), 113.8 (d, J = 26.3 Hz, C-4′), 112.0 (C-4a), 31.0 (NCH₃); HR-ESI-MS [M-Br]⁺: Calcd for C₁₈H₁₄FN₂⁺, 277.1136, found 277.1170.

9-Methyl-2-(4-fluorophenyl)-β-carboline bromide (B4): Yield, 76%; yellow powders; mp 296.8–297.8 °C; ¹H NMR (500 MHz, DMSO-d₆) δ 9.95 (1 H, s, H-1), 9.03 (1 H, d, J = 6.5 Hz, H-3), 8.99 (1 H, dd, J = 6.5, 1.3 Hz, H-2), 8.67 (1 H, d, J = 8.0 Hz, H-5), 8.08 (2 H, dd, J = 8.8, 4.5 Hz, H-2′, H-6′), 8.01–7.93 (2 H, m, H-7, H-8), 7.67 (2 H, t, J = 8.7 Hz, H-3′, H-5′), 7.57 (1 H, 2 × t, J = 8.0, 1.3 Hz, H-6), 4.19 (3 H, s, NCH₃). ¹³C NMR (126 MHz, DMSO-d₆) δ 162.9 (d, J = 249.0 Hz, C-4′), 145.2 (C-1), 140.0 (d, J = 2.9 Hz, C-1′), 136.0 (C-8a), 132.9 (C-3), 132.6 (C-9a), 132.1 (C-4b), 129.4 (C-4), 127.7 (d, J = 9.4 Hz, C-2′, C-6′), 124.1 (C-7), 122.1 (C-5), 118.9 (C-6), 117.6 (C-8), 116.9 (d, J = 23.6 Hz, C-3′, C-5′), 111.4 (C-4a), 30.5 (NCH₃); HR-ESI-MS [M-Br]⁺: Calcd for C₁₈H₁₄FN₂⁺, 277.1136, found 277.1118.

9-Methyl-2-(2-chlorophenyl)-β-carboline bromide (B5): Yield, 36%; golden yellow powders; mp 206.9–207.7 °C; ¹H NMR (500 MHz, DMSO-d₆) δ 9.99 (1 H, d, J = 1.1 Hz, H-1), 9.10 (1 H, d, J = 6.5 Hz, H-4), 8.96 (1 H, dd-like, J = 6.5, 1.4 Hz, H-4), 8.69 (1 H, d, J = 8.0 Hz, H-5), 8.04 (dd, J = 7.8, 1.4 Hz, H-6′), 8.02–7.96 (2 H, m, H-7, H-8), 7.94 (1 H, d, J = 8.0, 1.4 Hz, H-3′), 7.82 (1 H, 2 × t, J = 7.8, 1.6 Hz, H-4′), 7.77 (1 H, 2 × t, J = 7.7, 1.5 Hz, H-5′), 7.59 (1 H, dt-like, J = 8.0, 1.5 Hz, H-6), 4.17 (3 H, s, NCH₃); ¹³C NMR (126 MHz, DMSO-d₆) δ 145.4 (C-1), 140.6 (C-1′), 135.8 (C-8a), 134.0 (C-2′), 133.0 (C-3), 132.8 (C-9a), 132.6 (C-4b), 130.6 (C-4), 130.4 (C-4′), 128.9 (C-3′), 128.8 (C-5′), 128.6 (C-6′), 124.3 (C-7), 122.2 (C-5), 119.0 (C-6), 117.7 (C-8), 111.6 (C-4a), 30.7 (NCH₃). HR-ESI-MS [M-Br]⁺: Calcd for C₁₈H₁₄ClN₂⁺, 293.0840, found 293.0848.

9-Methyl-2-(3-chlorophenyl)-β-carboline bromide (B6): Yield, 60%; orangered powders; mp 206.7–207.5 °C; ¹H NMR (500 MHz, DMSO-d₆) δ 9.97 (1 H, d, J = 1.1 Hz, H-1), 9.04 (2 H, broad s, H-3, H-4), 8.67 (1 H, d, J = 8.0 Hz, H-5), 8.23 (1 H, t, J = 2.1 Hz, H-2′), 8.01–7.94 (3 H, m, H-7, H-8, H-6′), 7.86 (1 H, 2 × t, J = 8.2, 1.6 Hz, H-4′), 7.83 (1 H, t, J = 7.9 Hz, H-5′), 7.57 (1 H, t, J = 6.6 Hz, H-6), 4.19 (3 H, s, NCH₃); ¹³C NMR (126 MHz, DMSO-d₆) δ 145.3 (C-1), 144.4 (C-1′), 140.1 (C-3′), 135.9 (C-8a), 134.1 (C-5′), 132.8 (C-3), 132.3 (C-9a), 131.6 (C-4b), 130.6 (C-2′), 129.2 (C-4), 125.5 (C-4′), 124.2 (C-7), 124.1 (C-6′), 122.1 (C-5), 118.8 (C-6), 117.6 (C-8), 111.4 (C-4a), 30.5 (NCH₃); HR-ESI-MS [M-Br]⁺: Calcd for C₁₈H₁₄ClN₂⁺, 293.0840, found 293.0847.

9-Methyl-2-(4-chlorophenyl)-β-carboline bromide (B7): Yield, 89%; golden yellow powders; mp 265.5–266.5 °C; ¹H NMR (500 MHz, DMSO-d₆) δ 9.94 (1 H, d, J = 1.2 Hz, H-1), 9.03 (1 H, d, J = 6.5 Hz, H-4), 9.00 (1 H, dd, J = 6.5, 1.2 Hz, H-3), 8.66 (1 H, d, J = 8.0 Hz, H-5), 8.05 (2 H, d, J = 8.7 Hz, H-3′, H-5′H-2′, H-6′), 8.01–7.92 (2 H, m, H-7, H-8), 7.90 (2 H, d, J = 8.7 Hz, H-2′, H-6′), 7.56 (1 H, t, J = 7.3 Hz, H-6), 4.19 (3 H, s, NCH₃). ¹³C NMR (126 MHz, DMSO-d₆) δ 145.2 (C-1), 142.3 (C-1′), 136.0 (C-8a), 135.5 (C-4′), 132.8 (C-3), 132.7 (C-3′, C-5′), 132.2 (C-9a), 130.0 (C-4b), 129.2 (C-4), 127.2 (C-2′, C-6′), 124.1 (C-7), 122.1 (C-5), 118.9 (C-6), 117.7 (C-8), 111.5 (C-4a), 30.6 (NCH₃); HR-ESI-MS [M-Br]⁺: Calcd for C₁₈H₁₄ClN₂⁺, 293.0840, found 293.0933; Negative ESI-MS m/z: 78.68 [⁷⁹Br⁻], 80.70 [⁸¹Br⁻].

9-Methyl-2-(2-bromophenyl)-β-carboline bromide (B8): Yield, 32%; golden yellow powders; mp 212.6–213.5 °C; ¹H NMR (500 MHz, DMSO-d₆) δ 10.00 (1 H, d, J = 1.3 Hz, H-1), 9.12 (1 H, d, J = 6.4 Hz, H-4), 8.93 (1 H, dd, J = 6.4, 1.4 Hz, H-3), 8.70 (1 H, d, J = 8.1 Hz, H-5), 8.07 (1 H, dd, J = 8.1, 1.4 Hz, H-6′), 8.03 (1 H, dd, J = 8.0, 1.6 Hz, H-2′), 8.02–7.96 (2 H, m, H-7, H-8), 7.80 (1 H, 2 × t, J = 7.8, 1.4 Hz, H-4′), 7.74 (1 H, 2 × t, J = 7.8, 1.6 H, H-5′), 7.59 (1 H, 2 × t, J = 8.0, 1.4 Hz, H-6), 4.17(3 H, s, NCH₃); ¹³C NMR (126 MHz, DMSO-d₆) δ 145.9 (C-1), 142.7 (C-1′), 136.3 (C-8a), 134.6 (C-4′), 134.2 (C-3′), 133.5 (C-3), 133.4 (C-5′), 133.2 (C-9a), 130.8 (C-4b), 129.9 (C-4), 129.2 (C-6′), 124.8 (C-7), 122.8 (C-5), 119.4 (C-2′), 119.3 (C-6), 118.1 (C-8), 112.1 (C-4a), 31.1 (NCH₃); HR-ESI-MS [M-Br]⁺: Calcd for C₁₈H₁₄BrN₂⁺, 337.0335 (⁷⁹Br), 339.0314 (⁸¹Br), found 337.0340, 339.0329.

9-Methyl-2-(3-bromophenyl)-β-carboline bromide (B9): Yield, 53%; yellow powders; mp 219.2–220.2 °C; ¹H NMR (500 MHz, DMSO-d₆) δ 9.96 (1 H, s, H-1), 9.03 (2 H, s, H-3, H-4), 8.66 (1 H, d, J = 8.0 Hz, H-5), 8.33 (1 H, d, J = 2.1 Hz, H-2′), 8.02 (1 H, dd, J = 8.2, 2.1 Hz, H-4′), 8.00–7.94 (3 H, m, H-7, H-8, H-6′), 7.75 (1 H, t, J = 8.1 Hz, H-5), 7.57 (1 H, t, J = 7.3 Hz, H-6), 4.19 (3 H, s, NCH₃); ¹³C NMR (125 MHz, DMSO-d₆) δ 145.8 (C-1), 145.0 (C-1′), 136.4 (C-8a), 134.1 (C-6′), 133.32 (C-5′), 133.30 (C-3), 132.8 (C-9a), 132.4 (C-4b), 129.8 (C-4), 128.7 (C-4′), 125.1 (C-3′), 124.6 (C-7), 122.8 (C-2′), 122.7 (C-5), 119.4 (C-6), 118.1 (C-8), 112.0 (C-4a), 31.0 (NCH₃). HR-ESI-MS [M-Br]⁺: Calcd for C₁₈H₁₄BrN₂⁺, 337.0335 (⁷⁹Br), 339.0314 (⁸¹Br), found 337.0342, 339.0323.

9-Methyl-2-(4-bromophenyl)-β-carboline bromide (B10): Yield, 80%; faint yellow powders; mp 260.0–261.1 °C; ¹H NMR (500 MHz, DMSO-d₆) δ 9.93 (1 H, d, J = 1.4 Hz, H-1), 9.03 (1 H, d, J = 6.5 Hz, H-3), 9.00 (1 H, dd, J = 6.5, 1.4 Hz, H-4), 8.66 (1 H, d, J = 8.0 Hz, H-5), 8.03 (2 H, d, J = 8.8 Hz, H-2′, H-6′), 7.97 (2 H, d, J = 8.8 Hz, H-3′, H-5′), 7.99–7.93 (2 H, m, H-7, H-8), 7.57 (1 H, 2 × t, J = 8.0, 1.4 Hz, H-6), 4.18 (3 H, s, NCH₃); ¹³C NMR (126 MHz, DMSO-d₆) δ 145.3 (C-1), 142.7 (C-1′), 136.0 (C-8a), 133.0 (C-3), 132.8 (C-3′), 132.7 (C-5′), 132.2 (C-9a), 131.3 (C-4b), 129.2 (C-4), 127.8 (C-3′), 127.4 (C-5′), 124.2 (C-2′), 124.1 (C-6′), 122.2 (C-5), 118.9 (C-6), 117.7 (C-8), 111.5 (C-4a), 30.6 (NCH₃). HR-ESI-MS [M-Br]⁺: Calcd for C₁₈H₁₄BrN₂⁺, 337.0335 (⁷⁹Br), 339.0314 (⁸¹Br), found 337.0337, 339.0316.

9-Methyl-2-(2-iodohenyl)-β-carboline bromide (B11): Yield, 34%; golden yellow powders; mp 253.6–254.4 °C; ¹H NMR (500 MHz, DMSO-d₆) δ 9.92 (1 H, d, J = 1.2 Hz, H-1), 9.09 (1 H, d, J = 6.4 Hz, H-4), 8.87 (1 H, dd, J = 6.4, 1.4 Hz, H-3), 8.68 (1 H, d, J = 8.1 Hz, H-5), 8.23 (1 H, dd, J = 7.9, 1.4 Hz, H-6′), 8.02–7.96 (2 H, m, H-7, H-8), 7.93 (1 H, dd, J = 7.9, 1.5 Hz, H-2′), 7.78 (1 H, td, J = 7.7, 1.4 Hz, H-4′), 7.60 (1 H, td, J = 8.0, 2.0 Hz, H-6), 7.53 (1 H, td, J = 7.8, 1.5 Hz, H-5′), 4.17 (3 H, s, NCH₃); ¹³C NMR (126 MHz, DMSO-d₆) δ 146.4 (C-1′), 145.9 (C-1), 140.2 (C-3′), 136.3 (C-8a), 134.6 (C-3), 133.5 (C-9a), 133.15 (C-4′), 133.12 (C-4b), 130.6 (C-5′), 130.4 (C-4), 128.2 (C-6′), 124.7 (C-7), 122.8 (C-5), 119.4 (C-6), 118.2 (C-8), 112.1 (C-4a), 96.7 (C-2′), 31.1 (NCH₃); HR-ESI-MS [M-Br]⁺: Calcd for C₁₈H₁₄IN₂⁺, 385.0196, found 385.0198.

9-Methyl-2-(3-iodohenyl)-β-carboline bromide (B12): Yield, 60%; brown red powders; mp 243.7–244.0 °C; ¹H NMR (500 MHz, DMSO-d₆) δ 9.93 (1 H, d, J = 1.1 Hz, H-1), 9.01(1 H, d, J = 6.5 Hz, H-4), 9.00 (1 H, dd, J = 6.5, 1.1 Hz, H-3), 8.66 (1 H, d, J = 8.0 Hz, H-5), 8.42 (1 H, t, J = 1.9 Hz, H-2′), 8.13 (1 H, d, J = 8.2 Hz, H-6′), 8.01 (1 H, dd, J = 8.1, 2.2 Hz, H-4′), 8.00–7.93 (2 H, m, H-7, H-8), 7.60–7.54 (2 H, m, H-6, H-5′), 4.19 (3 H, s, NCH₃); ¹³C NMR (126 MHz, DMSO-d₆) δ 145.8 (C-1′), 144.8 (C-1), 139.9 (C-4′), 136.4 (C-8a), 134.0 (C-2′), 133.3 (C-5′), 133.2 (C-9a), 132.7 (C-3), 132.2 (C-4b), 129.7 (C-4), 125.3 (C-6′), 124.6 (C-7), 122.6 (C-5), 119.4 (C-6), 118.1 (C-8), 112.0 (C-4a), 96.0 (C-3′), 31.0 (NCH₃); HR-ESI-MS [M-Br]⁺: Calcd for C₁₈H₁₄IN₂⁺, 385.0196, found 385.0188.

9-Methyl-2-(4-iodohenyl)-β-carboline bromide (B13): Yield, 92%; orangered powders; mp 281.6–282.8 °C; ¹H NMR (500 MHz, DMSO-d₆) δ 9.92 (1 H, d, J = 1.3 Hz, H-1), 9.02 (1 H, d, J = 6.5 Hz, H-4), 8.98 (1 H, dd, J = 6.5, 1.4 Hz, H-3), 8.65 (1 H, dd, J = 8.0, 1.0 Hz, H-5), 8.18 (2 H, d, J = 8.7 Hz, H-3′, H-5′), 7.99–7.93 (2 H, m, H-7, H-8), 7.80 (2 H, d, J = 8.7 Hz, H-2′, H-6′), 7.56 (1 H, 2 × t, J = 8.0, 1.4 Hz, H-6), 4.18 (3 H, s, NCH₃); ¹³C NMR (126 MHz, DMSO-d₆) δ 145.2 (C-1), 143.2 (C-1′), 138.8 (C-3′, H-5′), 136.0 (C-8a), 132.7 (C-3), 132.5 (C-9a), 132.1 (C-4b), 129.0 (C-4), 127.2, 124.1 (C-7), 122.1 (C-5), 118.9 (C-6), 117.7 (C-8), 111.4 (C-4a), 97.7 (C-4′), 30.5 (NCH₃); HR-ESI-MS [M-Br]⁺: Calcd for C₁₈H₁₄IN₂⁺, 385.0196, found 385.0150; Negative ESI-MS m/z: 78.69 [⁷⁹Br⁻], 80.68 [⁸¹Br⁻].

9-Methyl-2-(2-hydroxyhenyl)-β-carboline bromide (B14): Yield, 33%; red brown powders; mp 189.7–191.2 °C; ¹H NMR (500 MHz, DMSO-d₆) δ 9.72 (1 H, s, H-1), 8.82 (1 H, d, J = 6.4 Hz, H-4), 8.76 (1 H, d, J = 6.5 Hz, H-3), 8.59 (1 H, d, J = 8.0 Hz, H-5), 7.94 (1 H, d, J = 8.4 Hz, H-8), 7.89 (1 H, 2 × t, J = 8.4, 2.1 Hz, H-7), 7.56–7.48 (2 H, m, H-4′), 7.33 (1 H, dd, J = 7.6, 2.0 Hz, H-6′), 7.15 (1 H, d, J = 7.9 Hz, H-3′), 7.07 (1 H, 2 × t, J = 8.0, 1.8 Hz, H-5′), 6.58 (1 H, 2 × broad s, OH), 4.14 (s, 3 H, NCH₃); ¹³C NMR (126 MHz, DMSO-d₆) δ 145.1 (C-2′), 136.1 (C-1), 134.1 (C-8a), 131.9 (C-3), 131.4 (C-9a), 130.8 (C-4b), 129.6 (C-4′), 128.4 (C-3), 125.9 (C-6′), 125.5 (C-1′), 124.6 (C-7), 124.0 (C-5′), 121.9 (C-5), 119.6 (C-6), 117.0 (C-8), 112.4 (C-3′), 111.5 (C-4a), 30.6 (NCH₃). HR-ESI-MS [M-Br]⁺: Calcd for C₁₈H₁₅N₂O⁺, 275.1179, found 275.1178.

9-Methyl-2-(3-hydroxyhenyl)-β-carboline bromide (B15): Yield, 69%; red brown powders; mp 233.8–235.1 °C; ¹H NMR (500 MHz, DMSO-d₆) δ 10.42 (1 H, s, OH), 9.89 (1 H, s, H-1), 8.99 (1 H, d, J = 6.5 Hz, H-4), 8.96 (1 H, dd, J = 6.5, 1.3 Hz, H-3), 8.65 (1 H, d, J = 8.1 Hz, H-5), 8.04–7.89 (2 H, m, H-7, H-8), 7.58 (1 H, t, J = 8.0 Hz, H-5′), 7.54 (1 H, 2 × t, J = 8.0, 1.4 Hz, H-6), 7.38 (1 H, dd, J = 7.8, 2.1 Hz, H-6′), 7.35 (1 H, t, J = 2.3 Hz, H-2′), 7.17 (1 H, dd, J = 8.2, 2.2 Hz, H-4′), 4.18 (3 H, s, NCH₃); ¹³C NMR (126 MHz, DMSO-d₆) δ 158.5 (C-3′), 145.1 (C-1), 144.5 (C-1′), 136.0 (C-8a), 132.52 (C-9a), 132.48 (C-3), 132.0 (C-4b), 130.9 (C-5′), 128.8 (C-4), 124.0 (C-7), 121.9 (C-5), 118.8 (C-6), 117.6 (C-8), 117.5 (C-6′), 115.3 (C-4′), 112.1 (C-4a), 111.3 (C-2′), 30.4 (NCH₃); HR-ESI-MS [M-Br]⁺: Calcd for C₁₈H₁₅N₂O⁺, 275.1179, found 275.1190.

9-Methyl-2-(4-hydroxyhenyl)-β-carboline bromide (B16): Yield, 93%; red brown powders; mp 267.9–268.5 °C; ¹H NMR (500 MHz, DMSO-d₆) δ 9.72 (1 H, s, H-1), 8.88 (1 H, d, J = 6.6 Hz, H-4), 8.86 (1 H, d, J = 6.6 Hz, H-3), 8.60 (1 H, d, J = 8.0 Hz, H-5), 7.95–7.88 (2 H, m, H-7, H-8), 7.56 (2 H, d, J = 8.1 Hz, H-2′, H-6′), 7.53 (1 H, t, J = 7.3 Hz, H-6), 6.71 (2 H, d, J = 8.1 Hz, H-3′, H-5′), 4.18 (1 H, b s, OH), 4.18 (3 H, s, NCH₃). ¹³C NMR (126 MHz, DMSO-d₆) δ 144.7 (C-1), 136.3 (C-4′), 132.1 (C-1′), 132.0 (C-8a), 131.8 (C-3), 130.4 (C-9a), 127.5 (C-4), 125.4 (C-4b), 125.33 (C-2′), 125.31 (C-6′), 123.7 (C-7), 121.7 (C-5), 119.0 (C-6), 118.0 (C-3′), 117.9 (C-5′), 117.6 (C-8), 111.1 (C-4a), 30.4 (NCH₃); HR-ESI-MS [M-Br]⁺: Calcd for C₁₈H₁₅N₂O⁺, 275.1179, found 275.1185.

9-Methyl-2-(2-methoxylphenyl)-β-carboline bromide (B17): Yield, 46%; orange powders; mp 181.7–182.4 °C; ¹H NMR (500 MHz, DMSO-d₆) δ 9.85 (1 H, d, J = 1.3 Hz, H-1), 8.99 (1 H, d, J = 6.4 Hz, H-4), 8.84 (1 H, dd, J = 6.5, 1.3 Hz, H-3), 8.64 (1 H, d, J = 8.1 Hz, H-5), 8.00–7.94 (2 H, m, H-7, H-8), 7.81 (1 H, dd, J = 7.8, 1.6 Hz, H-6′), 7.74 (1 H, 2 × t, J = 8.0, 1.7 Hz, H-4′), 7.57 (1 H, 2 × t, J = 7.2, 1.7 Hz, H-6), 7.49 (1 H, dd, J = 8.5, 1.2 Hz, H-5′), 7.31 (1 H, 2 × t, J = 7.7, 1.2 Hz, H-3′), 4.15 (3 H, s, OCH₃), 3.86 (3 H, s, NCH₃); ¹³C NMR (125 MHz, DMSO-d₆) δ 152.9 (C-2′), 145.7 (C-1), 136.4 (C-8a), 135.0 (C-4′), 133.2 (C-3), 133.0 (C-9a), 132.7 (C-4b), 131.1 (C-4), 127.9 (C-6′), 124.6 (C-7, C-1′), 122.5 (C-5), 121.6 (C-5′), 119.4 (C-6), 117.7 (C-8), 113.9 (C-3′), 111.9 (C-4a), 57.0 (OCH₃), 31.0 (NCH₃); HR-ESI-MS [M-Br]⁺: Calcd for C₁₉H₁₇N₂O⁺, 289.1335, found 289.1367.

9-Methyl-2-(3-methoxylphenyl)-β-carboline bromide (B18): Yield, 70%; yellow powders; mp 204.0–205.1 °C; ¹¹H NMR (500 MHz, DMSO-d₆) δ 9.93 (1 H, s, H-1), 9.01 (2 H, s, H-3, H-4), 8.66 (1 H, d, J = 8.0 Hz, H-5), 8.03–7.92 (2 H, m, H-7, H-8), 7.70 (1 H, t, J = 8.2 Hz, H-5′), 7.63 (1 H, t, J = 2.2 Hz, H-2′), 7.61–7.52 (2 H, m, H-6, H-6′), 7.33 (1 H, dd, J = 8.4, 2.4 Hz, H-4′), 4.19 (3 H, s, OCH₃), 3.93 (3 H, s, NCH₃); ¹³C NMR (126 MHz, DMSO-d₆) δ 160.6 (C-3′), 145.7 (C-1), 145.1 (C-1′), 136.5 (C-8a), 133.3 (C-5′), 133.2 (C-9a), 132.7 (C-3), 131.5 (C-4b), 129.6 (C-4), 124.6 (C-7), 122.6 (C-5), 119.4 (C-6′), 118.2 (C-6), 117.6 (C-8), 116.9 (C-2′), 111.9 (C-4′), 111.7(C-4a), 56.5 (OCH₃), 31.1 (NCH₃); HR-ESI-MS [M-Br]⁺: Calcd for C₁₉H₁₇N₂O⁺, 289.1335, found 289.1340.

9-Methyl-2-(4-methoxylphenyl)-β-carboline bromide (B19): Yield, 93%; faint yellow powders; mp 225.5–226.8 °C; ¹¹H NMR (500 MHz, DMSO-d₆) δ 9.87 (1 H, d, J = 1.3 Hz, H-1), 8.99 (1 H, d, J = 6.5 Hz, H-4), 8.95 (1 H, dd, J = 6.5, 1.4 Hz, H-3), 8.65 (1 H, d, J = 8.0 Hz, H-5), 7.99–7.92 (2 H, m, H-7, H-8), 7.93 (2 H, d, J = 9.0 Hz, H-2′, H-6′), 7.55 (1 H, 2 × t, J = 8.0, 1.2 Hz, H-6), 7.32 (2 H, d, J = 9.0 Hz, H-3′, H-5′), 4.18 (3 H, s, OCH₃), 3.92 (3 H, s, NCH₃); ¹³C NMR (126 MHz, DMSO-d₆) δ 160.7 (C-4′), 145.1 (C-1), 136.8 (C-8a), 136.1 (C-1′), 132.8 (C-9a), 132.4 (C-3), 131.7 (C-4b), 129.0 (C-4), 126.4 (C-2′, C-6′), 124.0 (C-7), 122.0 (C-5), 118.9 (C-6), 117.7 (C-8), 115.1 (C-3′, C-5′), 111.3 (C-4a), 55.9 (OCH₃), 30.5 (NCH₃); HR-ESI-MS [M-Br]⁺: Calcd for C₁₉H₁₇N₂O⁺, 289.1335, found 289.1342; Negative ESI-MS m/z: 78.68 [⁷⁹Br⁻], 80.67 [⁸¹Br⁻].

9-Methyl-2-(2-methylphenyl)-β-carboline bromide (B20): Yield, 43%; yellow powders; mp 122.6–130.5 °C; ¹H NMR (500 MHz, DMSO-d₆) δ 9.83 (1 H, s, H-1), 9.03 (1 H, d, J = 6.3 Hz, H-4), 8.85 (1 H, d, J = 6.3 Hz, H-3), 8.68 (1 H, d, J = 8.0 Hz, H-5), 8.02–7.93 (2 H, m, H-7, H-8), 7.74 (1 H, d, J = 7.8 Hz, H-6′), 7.67 (1 H, t, J = 8.0 Hz, H-4′), 7.64 (1 H, t, J = 8.0 Hz, H-5′), 7.58 (2 H, m, H-6, H-3′), 4.16 (3 H, s, NCH₃), 2.17 (3 H, s, CH₃); ¹³C NMR (126 MHz, DMSO-d₆) δ 145.2 (C-1), 143.0 (C-1′), 136.0 (C-8a), 133.6 (C-9a), 133.0 (C-3), 132.6 (C-2′), 132.2 (C-4b), 131.5 (C-4), 130.9 (C-3′), 130.0 (C-4′), 127.4 (C-5′), 126.5 (C-6′), 124.1 (C-7), 122.0 (C-5), 119.0 (C-6), 117.6 (C-8), 111.4 (C-4a), 30.5 (NCH₃), 16.6 (CH₃); HR-ESI-MS [M-Br]⁺: Calcd for C₁₉H₁₇N₂⁺, 273.1386, found 273.1392.

9-Methyl-2-(3-methylphenyl)-β-carboline bromide (B21): Yield, 68%; luminous yellow powders; mp 227.9–229.3 °C; ¹H NMR (500 MHz, DMSO-d₆) δ 9.96 (1 H, s, H-1), 9.05 (1 H, d, J = 6.5 Hz, H-4), 9.03 (1 H, d, J = 6.7 Hz, H-3), 8.69 (1 H, d, J = 8.0 Hz, H-5), 8.06–7.94 (2 H, m, H-7, H-8), 7.87 (1 H, s, H-2′), 7.82 (1 H, d, J = 8.0 Hz, H-6′), 7.70 (1 H, t, J = 7.8 Hz, H-5′), 7.63–7.56 (2 H, m, H-6, H-4′), 4.23 (3 H, s, NCH₃), 2.54 (3 H, s, CH₃); ¹³C NMR (126 MHz, DMSO-d₆) δ 145.7 (C-1), 144.1 (C-1′), 140.6 (C-3′), 136.6 (C-8a), 133.2 (C-3), 133.1 (C-9a), 132.6 (C-4b), 131.7 (C-2′), 130.4 (C-4′), 129.5 (C-4), 126.0 (C-5′), 124.6 (C-7), 122.7 (C-6′), 122.6 (C-5), 119.4 (C-6), 118.2 (C-8), 111.9 (C-4a), 31.1 (NCH₃), 21.4 (CH₃); HR-ESI-MS [M-Br]⁺: Calcd for C₁₉H₁₇N₂⁺, 273.1386, found 273.1416.

9-Methyl-2-(4-methylphenyl)-β-carboline bromide (B22): Yield, 82%; orange powders; mp 290.2–290.9 °C; ¹H NMR (500 MHz, DMSO-d₆) δ 9.89 (1 H, s, H-1), 9.00 (1 H, d, J = 6.5 Hz, H-4), 8.97 (1 H, dd, J = 6.5, 1.4 Hz, H-3), 8.65 (1 H, d, J = 8.0 Hz, H-5), 8.00–7.92 (2 H, m, H-7, H-8), 7.88 (2 H, d, J = 8.3 Hz, H-2′, H-6′), 7.59 (2 H, d, J = 8.3 Hz, H-3′, H-5′), 7.56 (1 H, t, J = 8.1 Hz, H-6), 4.18 (3 H, s, NCH₃), 2.49 (3 H, s, CH₃); ¹³C NMR (126 MHz, DMSO-d₆) δ 145.1 (C-1), 141.3 (C-4′), 140.7 (C-1′), 136.1 (C-8a), 132.7 (C-9a), 132.5 (C-3), 131.9 (C-4b), 130.4 (C-3′, C-5′), 128.9 (C-4), 124.8 (C-2′, C-6′), 124.0 (C-7), 122.0 (C-5), 118.9 (C-6), 117.7 (C-8), 111.4 (C-4a), 30.5 (NCH₃), 20.6 (CH₃); HR-ESI-MS [M-Br]⁺: Calcd for C₁₉H₁₇N₂⁺, 273.1386, found 273.1390; Negative ESI-MS m/z: 78.72 [⁷⁹Br⁻], 80.72 [⁸¹Br⁻].

9-Methyl-2-(2-cyanophenyl)-β-carboline bromide (B23): Yield, 57%; brown yellow powders; mp 179.5–179.8 °C; ¹H NMR (500 MHz, DMSO-d₆) δ 10.08 (1 H, s, H-1), 9.14 (1 H, d, J = 6.5 Hz, H-4), 9.12 (1 H, d, J = 6.5 Hz, H-3), 8.68 (1 H, d, J = 8.0 Hz, H-5), 8.34 (1 H, d, J = 7.8 Hz, H-6′), 8.21–8.12 (2 H, m, H-3′, H-4′), 8.06–7.96 (3 H, m, H-7, H-8, H-5′), 7.61 (1 H, 2 × t-like, J = 8.0, 1.6 Hz, H-6), 4.18 (3 H, s, NCH₃); ¹³C NMR (126 MHz, DMSO-d₆) δ 145.6 (C-1), 144.3 (C-1′), 135.7 (C-8a), 135.0 (C-5′), 134.2 (C-3′), 133.5 (C-4′), 133.2 (C-9a), 132.9 (C-3), 131.8 (C-4b), 130.2 (C-3), 128.1 (C-6′), 124.3 (C-7), 122.4 (C-5), 118.9 (C-6), 117.6 (C-8), 114.8 (C≡N), 111.6 (C-4a), 109.4 (C-2′), 30.6 (NCH₃); HR-ESI-MS [M-Br]⁺: Calcd for C₁₉H₁₄N₃⁺, 284.1182, found 284.1193.

9-Methyl-2-(3-cyanophenyl)-β-carboline bromide (B24): Yield, 79%; orange-yellow powders; mp 271.0–272.8 °C; ¹H NMR (500 MHz, DMSO-d₆) δ 10.0 (1 H, s, H-1), 9.08 (1 H, s, H-4), 8.67 (1 H, d, J = 8.0 Hz, H-5), 8.63 (1 H, br s, H-3), 8.37 (1 H, dd, J = 8.2, 2.3 Hz, H-6′), 8.27 (1 H, d, J = 7.9 Hz, H-4′), 8.03 (1 H, d, J = 7.9 Hz, H-4′), 8.01–7.95 (2 H, m, H-7, H-8), 7.99 (1 H, s, H-3′), 7.58 (1 H, 2 × t, J = 8.0, 1.4 Hz, H-6), 4.20 (3 H, s, NCH₃); ¹³C NMR (126 MHz, DMSO-d₆) δ 147.0 (C-1′), 145.3 (C-1), 143.6 (C-4′), 135. 9 (C-8a), 134.3 (C-5′), 132.9 (C-9a), 132.7 (C-3), 132.4 (C-4b), 131.4 (C-6′), 130.3 (C-4), 129.3 (C-2′), 124.2 (C-7), 122.2 (C-5), 118.8 (c-6), 117.7 (C-8), 117.5 (C≡N), 112.7 (C-3′), 111.5 (C-4a), 30.5 (NCH₃); HR-ESI-MS [M-Br]⁺: Calcd for C₁₉H₁₄N₃⁺, 284.1182, found 284.1187.

9-Methyl-2-(4-cyanophenyl)-β-carboline bromide (B25): Yield, 74%; orange-yellow powders; mp 188.6–189.9 °C; ¹H NMR (500 MHz, DMSO-d₆) δ 10.0 (1 H, d, J = 1.2 Hz, H-1), 9.07 (1 H, d-like, J = 6.5 Hz, H-4), 9.04 (1 H, dd-like, J = 6.5, 1.2 Hz, H-3), 8.67 (1 H, d, J = 8.0 Hz, H-5), 8.34 (2 H, d, J = 8.7 Hz, H-2′, H-6′), 8.24 (2 H, d, J = 8.7 Hz, H-3′, H-5′), 8.03–7.94 (2 H, m, H-7, H-8), 7.58 (1 H, 2 × t, J = 8.0, 1.4 Hz, H-6), 4.20 (3 H, s, NCH₃); ¹³C NMR (126 MHz, DMSO-d₆) δ 146.4 (C-1′), 145.4 (C-1), 135.9 (C-8a), 134.2 (C-3′, C-5′), 132.9 (C-3), 132.6 (C-9a), 132.5 (C-4b), 129.2 (C-4), 126.6 (C-2′, C-6′), 124.2 (C-7), 122.2 (C-5), 118.9 (C-6), 117.74 (C-8), 117.71 (C-4′), 113.5 (C≡N), 111.5 (C-4a), 30.6 (NCH₃); HR-ESI-MS [M-Br]⁺: Calcd for C₁₉H₁₄N₃⁺, 284.1182, found 284.1180.

9-Methyl-2-(3-trifluoromethylphenyl)-β-carboline bromide (B26): Yield, 82%; orange-yellow powders; mp 125.4–126.1 °C; ¹H NMR (500 MHz, DMSO-d₆) δ 10.0 (1 H, s, H-1), 9.08 (1 H, dd, J = 6.5, 1.3 Hz, H-3), 9.06 (1 H, d, J = 6.5 Hz, H-4), 8.68 (1 H, d, J = 8.0 Hz, H-5), 8.48 (1 H, s, H-2′), 8.33 (1 H, dd, J = 8.0, 2.2 Hz, H-6′), 8.16 (1 H, d, J = 7.9 Hz, H-4′), 8.05 (1 H, t, J = 8.0 Hz, H-5′), 8.02–7.94 (2 H, m, H-7, H-8), 7.58 (1 H, 2 × t, J = 8.0, 1.3 Hz, H-6), 4.20 (3 H, s, NCH₃); ¹³C NMR (126 MHz, DMSO-d₆) δ 145.3 (C-1), 143.9 (C-1′), 135.9 (C-8a), 133.0 (C-3), 132.8 (C-9a), 132.3 (C-4b), 131.4 (C-4), 130.4 (q, J = 33.0 Hz, C-3′), 129.7 (C-5′), 129.5 (C-6′), 127.5 (q, J = 3.7 Hz, C-4′), 124.2 (C-7), 123.4 (q, J = 273.3 Hz, CF₃), 122.7 (q, J = 4.0 Hz, C-2′), 122.2 (C-5), 118.9 (C-6), 117.6 (C-8), 111.5 (C-4a), 30.6 (NCH₃); HR-ESI-MS [M-Br]⁺: Calcd for C₁₉H₁₄F₃N₂⁺, 327.1104, found 327.1081.

9-Methyl-2-(4-trifluoromethylphenyl)-β-carboline bromide (B27): Yield, 86%; yellow powders; mp 152.7–153.8 °C; ¹H NMR (500 MHz, DMSO-d₆) δ 10.0 (1 H, s, H-1), 9.07 (2 H, s, H-3, H-4), 8.67 (1 H, d, J = 8.0 Hz, H-5), 8.26 (2 H, d, J = 8.6 Hz, H-2′, H-6′), 8.22 (2 H, d, J = 8.6 Hz, H-3′, H-5′), 8.03–7.94 (2 H, m, H-7, H-8), 7.58 (1 H, 2 × t, J = 8.0, 1.4 Hz, H-6), 4.20 (3 H, s, NCH₃); ¹³C NMR (126 MHz, DMSO-d₆) δ 146.3 (d-like, J = 4.5 Hz, H-1′), 145.3 (C-1), 135.9 (C-8a), 132.9 (C-3), 132.7 (C-9a), 132.4 (C-4b), 130.8 (q, J = 32.3 Hz, C-4′), 129.3 (C-4), 127.3 (q, J = 3.8 Hz, C-3′, C-5′), 126.6 (C-2′, C-6′), 123.6 (q, J = 273.5 Hz, CF₃), 124.2 (C-7), 122.2 (C-5), 118.9 (C-6), 117.7 (C-8), 111.5 (C-4a), 30.6 (NCH₃); HR-ESI-MS [M-Br]⁺: Calcd for C₁₉H₁₄F₃N₂⁺, 327.1104, found 327.1130.

9-Methyl-2-(3-nitrophenyl)-β-carboline bromide (B28): Yield, 79%; orange-yellow powders; mp 182.2–183.5 °C; ¹H NMR (500 MHz, DMSO-d₆) δ 10.1 (1 H, d, J = 1.3 Hz, H-1), 9.09 (1 H, dd, J = 6.5, 1.4 Hz, H-3), 9.07 (1 H, d, J = 6.5 Hz, H-4), 8.96 (1 H, t, J = 2.3 Hz, H-2′), 8.68 (1 H, d, J = 8.1 Hz, H-5), 8.61 (1 H, dd, J = 8.2, 2.1 Hz, H-6′), 8.48 (1 H, dd, J = 7.8, 2.2 Hz, H-4′), 8.10 (1 H, t, J = 8.2 Hz, H-5′), 8.04–7.95 (2 H, m, H-7, H-8), 7.58 (1 H, dt-like, J = 8.0, 1.3 Hz, H-6), 4.21 (3 H, s, NCH₃); ³C NMR (126 MHz, DMSO-d₆) δ 148.2 (C-3′), 145.4 (C-1), 143.8 (C-1′), 135.9 (C-8a), 132.92 (C-3), 132.89 (C-9a), 132.5 (C-4b), 132.0 (C-5′), 131.6 (C-6′), 129.6 (C-4), 125.4 (C-4′), 124.2 (C-7), 122.2 (C-5), 121.0 (C-2′), 118.9 (C-6), 117.7 (C-8), 111.5 (C-4a), 30.6 (NCH₃); HR-ESI-MS [M-Br]⁺: Calcd for C₁₈H₁₄N₃O₂⁺, 304.1081, found 304.1089.

9-Methyl-2-(2,6-difluorophenyl)-β-carboline bromide (B29): Yield, 68%; yellow powders; mp 259.5–261.0 °C; ¹H NMR (500 MHz, DMSO-d₆) δ 10.10 (1 H, s, H-1), 9.15 (1 H, d, J = 6.5 Hz, H-4), 9.06 (1 H, d, J = 6.4 Hz, H-3), 8.67 (1 H, d, J = 8.1 Hz, H-5), 8.03–7.98 (2 H, m, H-7, H-8), 7.92 (1 H, nonet, J = 8.7, 6.3 Hz, H-4′), 7.66 (2 H, t, J = 8.8 Hz, H-3′, H-5′), 7.60 (1 H, dt-like, J = 8.0, 2.2 Hz, H-6), 4.14 (3 H, s, NCH₃); ¹³C NMR (126 MHz, DMSO-d₆) δ 156.1 (d, J = 254.2 Hz, C-2′, C-6′), 145.8 (C-1), 136.0 (C-8a), 134.4 (C-3), 133.8 (C-9a), 133.5 (C-4b), 133.2 (C-4), 131.2 (C-4′), 124.4 (C-7), 122.4 (C-5), 119.0 (C-6), 118.0 (C-8), 113.6 (d, J = 12.3 Hz, C-1′), 113.2 (dd, J = 18.8, 3.2 Hz, C-3′, C-5′), 111.7 (C-4a), 30.6 (NCH₃); HR-ESI-MS [M-Br]⁺: Calcd for C₁₈H₁₃F₂N₂⁺, 295.1041, found 295.1049.

9-Methyl-2-(2,4-dichlorophenyl)-β-carboline bromide (B30): Yield, 63%; brown powders; mp 215.6–216.8 °C; ¹H NMR (500 MHz, DMSO-d₆) δ 9.97 (1 H, d, J = 1.3 Hz, H-1), 9.10 (1 H, d, J = 6.4 Hz, H-4), 8.94 (1 H, dd, J = 6.4, 1.3 Hz, H-3), 8.67 (1 H, d, J = 8.0 Hz, H-5), 8.21 (1 H, d, J = 2.3 Hz, H-3′), 8.07 (1 H, d, J = 8.5 Hz, H-6′), 8.05–7.95 (2 H, m, H-7, H-8), 7.91 (1 H, dd, J = 8.5, 2.3 Hz, H-5′), 7.59 (1 H, dt-like, J = 8.0, 2.2 Hz, H-6), 4.15 (3 H, s, NCH₃); ¹³C NMR (126 MHz, DMSO-d₆) δ 145.5 (C-1), 139.5 (C-1′), 136.8 (C-4′), 136.6 (C-2′), 135.8 (C-8a), 133.9 (C-3), 133.1 (C-9a), 132.8 (C-4b), 130.4 (C-3′), 130.2 (C-5′), 130.1 (C-4), 128.9 (C-6′), 124.2 (C-7), 122.3 (C-5), 118.9 (C-6), 117.6 (C-8), 111.6 (C-4a), 30.6 (NCH₃); HR-ESI-MS [M-Br]⁺: Calcd for C₁₈H₁₃Cl₂N₂⁺, 327.0450 (³⁵Cl), 329.0421 (³⁷Cl) found 327.0458, 329.0428.

9-Methyl-2-(3,5-dichlorophenyl)-β-carboline bromide (B31): Yield, 73%; yellow powders; mp 272.6–273.8 °C; ¹H NMR (500 MHz, DMSO-d₆) δ 10.01 (1 H, s, H-1), 9.08 (2 H, t-like, J = 6.0 Hz, H-3, H-4), 8.69 (1 H, d, J = 8.0 Hz, H-5), 8.27 (2 H, d, J = 1.8 Hz, H-2′, H-6′), 8.13 (1 H, t, J = 1.8 Hz, H-4′), 8.08–7.94 (2 H, m, H-7, H-8), 7.61 (1 H, dt-like, J = 8.0, 1.6 Hz, H-6), 4.22 (3 H, s, NCH₃). ¹³C NMR (126 MHz, DMSO-d₆) δ 145.4 (C-1), 144.8 (C-1′), 135.8 (C-8a), 135.0 (C-3′, C-5′), 133.0 (C-3), 132.8 (C-9a), 132.5 (C-4b), 130.3 (C-4′), 129.3 (C-4), 124.8 (C-7), 124.2 (C-2′, C-6′), 122.2 (C-5), 118.8 (C-6), 117.6 (C-8), 111.5 (C-4a), 30.6 (NCH₃); HR-ESI-MS [M-Br]⁺: Calcd for C₁₈H₁₃Cl₂N₂⁺, 327.0450 (³⁵Cl), 329.0421 (³⁷Cl) found 327.0429, 329.0399.

9-Methyl-2-(2-fluoro-4-bromophenyl)-β-carboline bromide (B32): Yield, 56%; orange-yellow powders; mp 211.2–212.9 °C; ¹H NMR (500 MHz, DMSO-d₆) δ 9.97 (1 H, s, H-1), 9.07 (1 H, d, J = 6.5 Hz, H-4), 8.98 (1 H, dt, J = 6.4, 1.5 Hz, H-3), 8.66 (1 H, d, J = 8.0 Hz, H-5), 8.18 (1 H, dd, J = 9.9, 2.1 Hz, H-6′), 8.04–7.96 (3 H, m, H-7, H-8, H-5′), 7.89 (1 H, dt, J = 8.5, 1.5 Hz, H-5′), 7.59 (1 H, dt-like, J = 8.0, 1.7 Hz, H-6), 4.16 (3 H, s, NCH₃); ¹³C NMR (126 MHz, DMSO-d₆) δ 145.5 (C-1), 139.5 (C-1′), 136.8 (C-4′), 136.6 (C-2′), 135.8 (C-8a), 133.9 (C-3), 133.1 (C-9a), 132.8 (C-4b), 130.4 (C-3′), 130.2 (C-5′), 130.1 (C-4), 128.9 (C-6′), 124.2, 122.3, 118.9, 117.6 (C-8), 111.6 (C-4a), 30.6 (NCH₃); HR-ESI-MS [M-Br]⁺: Calcd for C₁₈H₁₃BrFN₂⁺, 355.0241 (⁷⁹Br), 357.0220 (⁸¹Br) found 355.0244, 357.0223.

9-Methyl-2-(2,4-dibromophenyl)-β-carboline bromide (B33): Yield, 55%; yellow powders; mp 258.4–258.7 °C; ¹H NMR (500 MHz, DMSO-d₆) δ 9.97 (1 H, d, J = 1.3 Hz, H-1), 9.10 (1 H, d, J = 6.4 Hz, H-4), 8.92 (1 H, dd, J = 6.4, 1.3 Hz, H-4), 8.68 (1 H, d, J = 8.1 Hz, H-5), 8.41 (1 H, d, J = 2.0 Hz, H-3′), 8.06 (1 H, dd, J = 8.5, 2.2 Hz, H-5′), 8.03–7.94 (3 H, m, H-7, H-8, H-6′), 7.59 (1 H, dt-like, J = 8.0, 1.7 Hz, H-6), 4.16 (3 H, s, NCH₃); ¹³C NMR (126 MHz, DMSO-d₆) δ 145.5 (C-1), 141.5 (C-1′), 135.7 (C-3′), 135.6 (C-8a), 133.9 (C-3), 133.1 (C-9a), 132.7 (C-4b), 132.3 (C-5′), 130.2 (C-6′), 130.1 (C-4), 125.1 (C-2′), 124.2 (C-7), 122.3 (C-5), 120.4 (C-4′), 118.9 (C-6), 117.6 (C-8), 111.6 (C-4a), 30.6 (NCH₃); HR-ESI-MS [M-Br]⁺: Calcd for C₁₈H₁₃Br₂N₂⁺, 414.9440 (2 × ⁷⁹Br), 416.9420 (⁷⁹Br + ⁸¹Br), 418.9399 (2 × ⁸¹Br) found 414.9439, 416.9418, 418.9398.

6-Methoxy-9-methyl-2-phenyl-β-carboline bromide (B34): Yield, 88%; orange-yellow powders; ¹H NMR (500 MHz, MeOD) δ 9.64 (1 H, s, H-1), 8.82 (1 H, d, J = 6.5 Hz, H-4), 8.73 (1 H, d, J = 6.5 Hz, H-3), 8.00 (1 H, d, J = 2.6 Hz, H-5), 7.91 (2 H, d-like, J = 6.6 Hz), 7.82 (1 H, d, J = 9.2 Hz), 7.78–7.74 (3 H, m), 7.58 (1 H, dd, J = 9.2, 2.7 Hz), 4.17 (NCH₃), 3.99 (OCH₃); ¹³C NMR (126 MHz, CD₃OD) δ 157.5 (C-6), 145.6 (C-1), 142.8 (C-1′), 138.0, 133.8, 132.9, 132.1, 131.6 (C-3′, C-5′), 129.5, 126.1 (C-2′, C-6′), 125.8, 121.2, 118.7, 113.3, 104.6, 56.6 (OCH₃), 30.9 (NCH₃); HR-ESI-MS [M-Br]⁺: Calcd for C₂₁H₂₁N₂⁺, 289.1335 found 289.1342.

6,9-Dimethyl-2-phenyl-β-carboline bromide (B35): Yield, 90%; orange-yellow powders; ¹H NMR (500 MHz, MeOD) δ 9.66 (1 H, s, H-1), 8.81(1 H, d, J = 6.5 Hz, H-4), 8.79 (1 H, d, J = 6.5 Hz, H-3), 8.34 (1 H, s, H-5), 7.94 (2 H, d, J = 6.9 Hz, H-2′, H-6′), 7.79–7.82 (5 H, m), 4.19 (3 H, NCH₃), 2.63 (3 H, CH₃); ¹³C NMR (126 MHz, MeOD) δ 145.9 (C-1), 145.6 (C-1′), 138.1, 136.1, 134.2, 133.9, 133.5, 132.1, 131.6 (C-3′, C-5′), 129.3, 126.1 (C-2′, C-6′), 124.0, 120.9, 118.6, 111.9, 30.9 (NCH₃), 21.4 (CH₃); HR-ESI-MS [M-Br]⁺: Calcd for C₁₉H₁₇N₂⁺, 273.1386 found 273.1393.

7-Fluoro-9-methyl-2-phenyl-β-carboline bromide (B36): Yield, 93%; orange-yellow powders; ¹H NMR (500 MHz, CD₃Cl) δ 9.94 (1 H, s, H-1), 9.06 (1 H, dd, J = 6.5, 1.2 Hz, H-3), 9.02 (1 H, d, J = 6.5 Hz, H-4), 8.75 (1 H, dd, J = 8.5, 5.5 Hz, H-5), 8.01 (2 H, d-like, J = 8.0 Hz, H-8), 7.94 (1 H, dd, J = 10.0, 2.0 Hz, H-8), 7.84–7.78 (3 H, m), 7.50–7.47 (1 H, m), 4.19 (3 H, s, CH₃); ¹³C NMR (126 MHz, DMSO-d₆) δ 165.2 (d, J = 249.2 Hz, C-7), 146.6 (d, J = 13.7 Hz, C-1), 143.6 (C-1′), 136.9 (d, J = 1.2 Hz, C-8a), 133.5 (C-3), 132.1 (C-4′), 130.8 (C-9a), 130.2 (C-3′, C-5′), 129.1, 128.9, 126.6 (d, J = 11.4 Hz, C-5), 125.5 (C-4), 125.2 (C-2′, C-6′), 117.6 (C-4b), 115.8 (C-4a), 111.3 (d, J = 25.7 Hz, C-8), 98.2 (d, J = 27.5 Hz, C-6), 30.9 (NCH₃); HR-ESI-MS [M-Br]⁺: Calcd for C₁₈H₁₄FN₂⁺, 277.1136 found 277.1145.

7-Chloro-9-methyl-2-phenyl-β-carboline bromide (B37): Yield, 93%; orange-yellow powders; ¹H NMR (500 MHz, CD₃Cl) δ 9.98 (1 H, s, H-1), 9.08–9.04 (2 H, m), 8.80 (1 H, d, J = 6.0 Hz, H-4), 8.70 (1 H, d, J = 8.5 Hz, H-5), 8.20 (1 H, d, J = 1.6 Hz, H-8), 8.03–8.01 (2 H, m), 7.84–7.78 (3 H, m), 7.63 (1 H, dd, J = 8.5, 1.7 Hz, H-6), 7.48 (1 H,d, J = 8.0 Hz, H-4′), 7.1.2 (1 H, d, J = 7.4 Hz, H-6), 4.20 (3 H, s, CH₃); ¹³C NMR (126 MHz, DMSO-d₆) δ 145.7 (C-1), 143.6 (C-1′), 136.7 (C-8a), 133.5, 130.8, 130.2 (C-3′, C-5′), 129.6, 128.0, 125.8, 125.5, 125.2 (C-2′, C-6′), 122.7, 118.0, 118.2 (C-8), 111.7 (C-4a), 30.8 (NCH₃); HR-ESI-MS [M-Br]⁺: Calcd for C₁₈H₁₄ClN₂⁺, 293.0840 found 293.0851.

9-Ethyl-2-phenyl-β-carboline bromide (B38): Yield, 90%; orange-yellow powders; ¹H NMR (500 MHz, CD₃Cl) δ 10.1 (1 H, s, H-1), 8.80 (1 H, d, J = 6.0 Hz, H-4), 8.71 (1 H, d, J = 6.0 Hz, H-3), 8.31 (1 H, d, J = 8.0 Hz, H-5), 8.06 (2 H, d, J = 7.5 Hz, H-2′, H-6′), 7.78 (1 H, t, J = 7.5 Hz), 7.61 (1 H, d, J = 8.4 Hz), 7.56 (2 H, t, J = 7.5 Hz, H-3′, H-5′), 7.48 (1 H, t, J = 7.5 Hz), 7.40 (1 H, t, J = 7.4 Hz, H-4′), 4.96 (2 H, q, J = 7.0 Hz, H-1″), 1.49 (3 H, t, J = 7.0 Hz, H-2″); ¹³C NMR (126 MHz, CD₃Cl) δ 144.7 (C-1), 143.1 (C-1′), 135.5 (C-8a), 133.0 (C-3), 132.9 (C-9a), 131.9 (C-4′), 130.7 (C-3′, C-5′), 130.5 (C-4b), 127.8 (C-4), 124.8 (C-7), 124.0 (C-2′, C-6′), 122.4 (C-5), 119.5 (C-6), 118.3 (C-8), 110.8 (C-4a), 40.2 (C-1″), 14.5 (C-2″); HR-ESI-MS [M-Br]⁺: Calcd for C₁₉H₁₇N₂⁺, 273.1386 found 273.1385.

9-Propyl-2-phenyl-β-carboline bromide (B39): Yield, 86%; orange-yellow powders; ¹H NMR (500 MHz, CD₃Cl) δ 10.1 (1 H, s, H-1), 8.81 (1 H, d, J = 6.0 Hz, H-4), 8.72 (1 H, d, J = 6.0 Hz, H-3), 8.30 (1 H, d, J = 7.8 Hz, H-5), 8.05 (2 H, d, J = 7.5 Hz, H-2′, H-6′), 7.77 (1 H, t, J = 7.4 Hz), 7.59 (1 H, d, J = 8.3 Hz), 7.54 (2 H, t, J = 7.4 Hz, H-3′, H-5′), 7.46 (1 H, t, J = 6.9 Hz), 7.38 (1 H, t, J = 7.4 Hz, H-4′), 4.86 (2 H, t, J = 6.7 Hz, H-1″), 1.97–1.87 (2 H, m, H-2″), 0.94 (3 H, t, J = 7.0 Hz, H-3″); ¹³C NMR (126 MHz, CD₃Cl) δ 145.2 (C-1), 143.1 (C-1′), 136.1, 132.92, 132.86, 132.0, 130.7, 130.4 (C-3′, C-5′), 127.9, 124.9 (C-2′, C-6′), 124.0, 122.4, 119.3, 118.4, 111.0, 46.5 (C-1″), 22.7 (C-2″), 11.5 (C-3″); HR-ESI-MS [M-Br]⁺: Calcd for C₂₀H₁₉N₂⁺, 287.1543 found 287.1538.

9-Iso-propyl-2-phenyl-β-carboline bromide (B40): Yield, 85%; orange-yellow powders; ¹H NMR (500 MHz, CDCl₃) δ 10.1 (1 H, s, H-1), 8.84 (1 H, d, J = 6.4 Hz, H-4), 8.76 (1 H, d, J = 6.4 Hz, H-3), 8.34 (1 H, d, J = 8.0 Hz, H-5), 8.06–8.04 (2 H, m), 7.82 (1 H, d, J = 8.5 Hz), 7.76 (1 H, t, J = 7.7 Hz), 7.55 (2 H, t, J = 7.7 Hz), 7.48 (1 H, t, J = 7.7 Hz), 7.40 (1 H, t, J = 7.5 Hz), 5.84 (1 H, p, J = 7.1 Hz, NCHMe₂), 1.77 (6 H, d, J = 6.9 Hz, 2 × CH₃); ¹³C NMR (126 MHz, CDCl₃) δ 143.9 (C-1), 143.3 (C-1′), 135.5 (C-8a), 133.0 (C-3), 132.6, 132.0, 130.8, 130.5 (C-3′, C-5′), 128.1, 124.9 (C-2′, C-6′), 124.2, 122.2, 120.3, 118.3 (C-8), 113.2 (C-4a), 49.8 (NCHMe₂), 21.3 (2 × CH₃); HR-ESI-MS [M-Br]⁺: Calcd for C₂₀H₁₉N₂⁺, 287.1543 found 287.1539.

9-Allyl-2-phenyl-β-carboline bromide (B41): Yield, 74%; orange-yellow powders; ¹H NMR (500 MHz, CD₃Cl) δ 10.1 (1 H, s, H-1), 8.82 (1 H, d, J = 6.0 Hz, H-4), 8.80 (1 H, d, J = 6.0 Hz, H-3), 8.32 (1 H, d, J = 8.0 Hz, H-5), 8.13 (2 H, d, J = 7.6 Hz, H-2′, H-6′), 7.79 (1 H, t, J = 7.6 Hz), 7.61–7.55 (3 H, m), 7.50 (1 H, t, J = 7.2 Hz), 7.43 (1 H, t, J = 7.5 Hz), 6.09–6.03 (1 H, m, H-2″), 5.70 (2 H, br s, H-1″), 5.17 (1 H, d, J = 10.3 Hz, H-3″a), 5.06 (1 H, d, J = 17.1 Hz, H-3″b); ¹³C NMR (126 MHz, CD₃Cl) δ 145.2 (C-1), 143.1 (C-1′), 135.8, 133.0, 132.04, 133.01, 131.3, 130.8, 130.5 (C-3′, C-5′), 128.4, 124.8 (C-2′, C-6′), 123.9, 122.6, 119.4, 118.4, 117.9 (C-3″), 111.2, 47.3 (C-1″); HR-ESI-MS [M-Br]⁺: Calcd for C₂₀H₁₇N₂⁺, 285.1386 found 285.1383.

9-Butyl-2-phenyl-β-carboline bromide (B42): Yield, 83%; orange-yellow powders; ¹H NMR (500 MHz, CD₃Cl) δ 10.1 (1 H, s, H-1), 8.81 (1 H, d, J = 5.9 Hz, H-4), 8.72 (1 H, d, J = 5.9 Hz, H-3), 8.30 (1 H, d, J = 7.8 Hz, H-5), 8.05 (2 H, d, J = 7.3 Hz, H-2′, H-6′), 7.77 (1 H, t, J = 7.3 Hz), 7.59–7.53 (3 H, m), 7.46 (1 H, t, J = 7.1 Hz), 7.38 (1 H, t, J = 7.1 Hz), 4.88 (2 H, t, J = 6.5 Hz, H-1″), 1.88–1.80 (2 H, m, H-2″), 1.34–1.31 (2 H, m, H-3″), 0.85 (3 H, t, J = 6.9 Hz, H-3″); ¹³C NMR (126 MHz, CD₃Cl) δ 145.1 (C-1), 143.1 (C-1′), 135.92, 132.87, 132.0, 130.7, 130.5 (C-3′, C-5′), 127.8, 124.8 (C-2′, C-6′), 124.0, 122.4, 119.3, 118.4, 111.0, 57.9 (C-1″), 31.4 (C-2″), 20.3 (C-3″), 13.8 (C-4″); HR-ESI-MS [M-Br]⁺: Calcd for C₂₁H₂₁N₂⁺, 301.1699 found 301.1696.

9-Iso-butyl-2-phenyl-β-carboline bromide (B43): Yield, 80%; orange-yellow powders; ¹H NMR (500 MHz, CD₃Cl) δ 10.1 (1 H, s, H-1), 8.84 (1 H, d, J = 5.7 Hz, H-4), 8.76 (1 H, d, J = 5.7 Hz, H-3), 8.30 (1 H, d, J = 7.8 Hz, H-5), 8.13 (2 H, d, J = 7.3 Hz, H-2′, H-6′), 7.75 (1 H, t, J = 7.3 Hz), 7.57 (1 H, d, J = 8.3 Hz), 7.52 (2 H, J = 7.1 Hz, H-3′, H-5′), 7.43 (1 H, t, J = 7.1 Hz), 7.37 (1 H, t, J = 7.1 Hz), 4.74 (2 H, d, J = 7.1 Hz, H-1″), 2.28–2.36 (1 H, m, H-2″), 0.93 (6 H, d, J = 6.0 Hz, 2 × CH₃); ¹³C NMR (126 MHz, CD₃Cl) δ 145.5 (C-1), 143.1 (C-1′), 136.3, 132.9, 132.8, 132.2, 130.7, 130.4 (C-3′, C-5′), 128.1, 124.9 (C-2′, C-6′), 123.9, 122.4, 119.2, 118.5, 111.3, 51.9 (C-1″), 29.2 (C-2″), 20.2 (2 × CH₃); HR-ESI-MS [M-Br]⁺: Calcd for C₂₁H₂₁N₂⁺, 301.1699 found 301.1697.

9-Benzyl-2-phenyl-β-carboline bromide (B44): Yield, 76%; orange-yellow powders; ¹H NMR (500 MHz, CD₃Cl) δ 10.2 (1 H, s, H-1), 8.69 (1 H, d, J = 6.3 Hz, H-3), 8.55 (1 H, d, J = 6.3 Hz, H-4), 8.37 (1 H, dd, J = 8.1 Hz), 8.16 (1 H, d, J = 7.7 Hz), 7.87 (1 H, d, J = 7.8 Hz), 7.74 (1 H, J = 8.6 Hz), 7.66 (2 H, t, J = 7.6 Hz), 7.60 (1 H, t, J = 7.4 Hz), 7.53 (1 H, t, J = 7.7 Hz), 7.34 (3 H, d-like, J = 7.1 Hz), 7.28 (3 H, d-like, J = 7.2 Hz), 6.43 (2 H, s, NCH₂); ¹³C NMR (126 MHz, CD₃Cl) δ 145.7 (C-1), 143.3 (C-1′), 136.0, 135.6, 133.3, 133.1, 131.4, 131.0, 130.6, 129.5, 129.0, 128.1, 127.0, 124.8, 123.7, 122.8, 119.4, 117.7, 111.4, 48.3 (NCH₂); HR-ESI-MS [M-Br]⁺: Calcd for C₂₄H₁₉N₂⁺, 335.1544 found 335.1543.

9-(Ethoxy-2-oxyethyl)-2-phenyl-β-carboline bromide (B45): Yield, 75%; orange-yellow powders; ¹H NMR (500 MHz, CD₃Cl) δ 10.5 (1 H, s, H-1), 8.59 (1 H, d, J = 6.1 Hz, H-3), 8.48 (1 H, d, J = 6.3 Hz, H-4), 8.31 (1 H, d, J = 7.8 Hz), 8.16 (2 H, d, J = 7.4 Hz), 7.86 (1 H, t, J = 7.6 Hz), 7.66 (2 H, t-like, J = 7.3 Hz), 7.59 (2 H, t-like, J = 8.8 Hz), 7.50 (1 H, t, J = 7.6 Hz), 6.17 (2 H, s, N-CH₂), 4.28 (2 H, q, J = 7.1 Hz, CH₂CH₃), 1.36 (3 H, t, J = 7.1 Hz, CH₃); ¹³C NMR (126 MHz, CDCl₃) δ 168.2 (C = O), 145.2 (C-1), 143.2 (C-1′), 136.7, 133.22, 133.17, 131.2, 131.0, 130.6, 130.5, 124.7, 123.6, 122.9, 119.6, 117.5, 110.7, 62.4 (OCH₂), 47.0 (NCH₂), 14.1 (CH₃); HR-ESI-MS [M-Br]⁺: Calcd for C₂₁H₁₉N₂O₂⁺, 331.1450 found 331.1441.

2-Phenyl-β-carboline bromide (B46): Yield 72%; yellow powders; mp 228.1–229.8 °C; The ¹H NMR, ¹³C NMR and MS data are consistent with those in the literature³³.

Synthesis of 9-methyl-2-p-toly-1,2,3,4-tetrahydro-β-carboline (C1)

To the solution of compound A22 (6.1 mmol) in 50 mL ethanol was slowly added NaBH₄ (0.347 g, 9.2 mmol) under stirring. After completion of the reaction by TLC, the solution was concentrated under reduced pressure. 100 mL water was added to the residue, extracted with EtOAc (2 × 60 mL), washed with brine and dried over anhydrous Na₂SO₄. After filtration, the solvent was removed under reduced pressure to yield compound C1 as white powders in 98% yield. Mp 118.4–118.7 °C; ¹H NMR (125 MHz, D₃COD) δ 7.52 (1 H, d, J = 7.8 Hz, H-8), 7.30 (1 H, d, J = 8.2 Hz, H-5), 7.20 (1 H, 2 × t, J = 8.1, 1.0 Hz, H-6), 7.11 (2 H, d, J = 8.5 Hz, H-3′, H-5′), 7.10 (1 H, 2 × t, J = 7.8, 0.9 Hz, H-7), 7.00 (2 H, d, J = 8.5 Hz, H-2′, H-6′), 4.38 (2 H, s, H-1), 3.69 (3 H, s, NCH₃), 3.60 (2 H, t, J = 5.6 Hz, H-3), 2.93 (2 H, t, J = 5.6 Hz, H-4), 2.30 (3 H, s, CH₃); ¹³C NMR (500 MHz, D₃COD) δ 149.1 (C-1′), 137.3 (C-8a), 133.2 (C-9a), 129.9 (C-4′), 129.3 (C-3′, C-5′), 126.8 (C-4b), 121.1 (C-7), 119.1 (C-6), 118.2 (C-5), 116.9 (C-8), 108.8 (C-2′, C-4′), 108.1 (C-4a), 48.7 (C-3), 46.6 (C-1), 29.4 (NCH₃), 21.8 (C-4), 20.6 (CH₃); HR-ESI-MS [M−H]⁺ calcd for C₁₉H₁₉N₂, 275.1543, found 275.1536.

Assay of AChE and BuChE

The inhibitory activities of the compounds against AChE were evaluated according to Ellman’s coupled enzyme assay⁴⁷ with the following modifications. 90 μL potassium phosphate buffer (PBS, 0.1 M, pH 7.4), 10 μL the solution of 2 units/mL AChE in 0.1 M PBS (pH 7.4) and 10 μL the solution of 200 μM test compounds in the mixed solution of methanol-PBS (pH 7.4) (1:9, V/V) were added to each well of a 96-well plate. The methanol-PBS solution was used as a blank control. After incubation at room temperature for 10 min, 60 μL the solution of 10 mM DTNB in PBS was added into each the well. The plate was placed on crushed ices and cooled for 20 min. To each the well was added 30 μL the solution of 7.5 mM ATCh in PBS (pH 7.4). After incubation at 37 °C for 40 min, the initial rate of the enzyme was analyzed by measuring absorbance at wavelength of 412 nm with a microplate reader (Molecular Devices Co., Ltd.). Each test was performed in triplicate. Inhibition rates of the enzyme were calculated relative to a control sample. The inhibitory activities against BuChE were measured as described above for AChE, using 0.7 unit/mL BuChE and 4 mM BuTCh instead of AChE and ATCh as the enzyme and substrate, respectively.

The compounds with higher initial activities were further assayed for IC₅₀ values according to the method described above. Based on the screening results, a series of test concentrations of the compound were set and tested for inhibition rate against AChE and BChE. The probit value of the average inhibition rate for each test concentration and the corresponding lg[concentration] were used to establish enzyme inhibition regression equation by the linear least-square fitting method. IC₅₀ values were calculated from the equations.

Duncan multiple comparison test was used to evaluate significant difference between the average inhibition rates of various compounds at the same concentration by using PRISM software ver. 5.0 (GraphPad Software Inc., San Diego, CA, USA).

Data

Data will be made available upon request to the corresponding authors.