Figure 2 | Scientific Reports

Figure 2

From: A dynamic allosteric pathway underlies Rad50 ABC ATPase function in DNA repair

Figure 2

Mutations reveal an allosteric network in Rad50NBD. (a) 2D 13C,1H methyl-TROSY HMQC spectral overlay of WT and mutant Mre11HLH-Rad50NBD recorded at 14.1 T and 50 °C. Arrows highlight side chain methyl groups experiencing CSPs. (b) Structure of Mre11HLH-Rad50NBD emphasizing side chain methyl groups whose CSPs upon mutation cluster according to CHESCA as described in the Methods. Clusters are listed in Supplementary Table S1 and were determined via the dendrogram in Supplementary Fig. S4. Methyl groups not affected by the mutations are not shown.

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