Table 1 Development of oocytes after ICSI rescue with sperm from Plcz1 KO or point mutant mice.

From: Sperm-borne phospholipase C zeta-1 ensures monospermic fertilization in mice

Sperm Supplemented mRNA (conc.) No. of eggs injected No. of 2-cell stage embryos No. of 2cell embryos transferred Pregnant/Recipients No. of pups born
WT None 72 69 57 3/3 21
KO mouse Plcz1 (2 ng/µl) 78 78 62 3/3 31
D210R mouse Plcz1 (2 ng/µl) 73 71 49 2/3 13
H435P mouse Plcz1 (2 ng/µl) 73 70 45 3/3 17
KO human PLCz1 (0.2 ng/µl) 51 51 29 2/2 9
  1. All offspring from Plcz1 KO, D210R, or H435P spermatozoa were confirmed to possess the Plcz1 KO, D210R, H435P allele by PCR.