Evolutionary relationships of ElBla2 and other MBLs. The evolutionary history was inferred using the Neighbor-Joining method54. The optimal tree with a total branch length of 11.09452999 is shown. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the Poisson correction method66 and represent the number of amino acid substitutions per site. The analysis involved 25 amino acid sequences of MBLs belonging to subgroup B1 (red), B2 (green) and B3 (purple). All ambiguous positions were removed for each sequence pair. There were a total of 336 positions in the final dataset. Evolutionary analyses were conducted in MEGA755.