Figure 1 | Scientific Reports

Figure 1

From: Evaluation of PD-L1 expression on vortex-isolated circulating tumor cells in metastatic lung cancer

Figure 1

Workflow for evaluation of PD-L1 expression on patient CTC and matched tumor biopsy. (A) CTC Workflow. Blood is collected from cancer patients and processed through Vortex technology to enrich for CTCs. Blood is diluted 10X with PBS and injected through the microfluidic device with syringe pumps. Purified cells are collected into a 96 well-plate, where they are stained with immunofluorescence markers and imaged. Fluorescence intensity can be analyzed and PD-L1 gene expression quantified. (B) Tumor biopsy workflow: In parallel of the CTC workflow, lung biopsies were analyzed for PD-L1 expression. While biopsy provides information on the intra-tumor heterogeneity, only the CTCs present in a blood draw can cover both intra and inter-tumor heterogeneity.

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