Cell proliferation in Chn2 deficient animals. (a–h) BrdU was injected into either early postnatal stages (P4 and P10, a,b,d,e) or adult (P35, g,h) and allowed to incorporate for two hours prior to animal collection. (c,f) Quantification of BrdU pulse-chase experiments. We found there is no significant difference in the density of proliferating, BrdU-positive GCPs in the oEGL between Chn2−/− mice and WT animals (n = 5 animals per genotype, per stage, 4–5 sections 4 μm-thick per animal, two-tail t-test; P4: p = 0.32; P10: p = 0.952). In adult (P35) animals, neuronal ectopias do not contain proliferating cells, suggesting that they are composed entirely of postmitotic cells (h). Scale bar, 50 μm for all. Error bars represent SEM.