Figure 7 | Scientific Reports

Figure 7

From: Loss of CD14 leads to disturbed epithelial-B cell crosstalk and impairment of the intestinal barrier after E. coli Nissle monoassociation

Figure 7

B cell contribution to the intestinal homeostasis. (a) Bacterial CFU determined 72 h post EcN monoassociation in spleen and liver of CXCR5−/− and CCR7−/− mice (n = 4). (b) Quantification of TUNEL stained cells in the ileum of CXCR5−/− and CCR7−/− mice before and after EcN stimulation (n = 4). (c) ZO-1, claudin 8 and occludin gene expression measured by qPCR in total RNA isolated from CXCR5−/− ileum. Relative differences in gene expression were calculated by the comparative 2−ΔΔCt method. Values are shown as fold induction (n = 4). (d) J chain gene expression measured by qPCR in the distal small intestine of CXCR5−/− mice. Relative differences in gene expression were calculated by the comparative 2−ΔΔCt method. Values are shown as fold induction (n = 4). (e) Quantification of granzyme B positive cells in the distal small intestine of CXCR5−/− mice (n = 4).

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