Figure 2 | Scientific Reports

Figure 2

From: Peptide Sequencing Directly on Solid Surfaces Using MALDI Mass Spectrometry

Figure 2

Selective derivatization of a peptide at its N-terminus for sequence analysis. (a) An 8 mer peptide was synthesized using BOC chemistry; the peptide’s side chains were deprotected by a conventional TMFSA Low-High procedure before it was derivatized with TMPP-Ac-OSu under controlled pH (8.0–8.5). (b) A few beads of the derivatized peptide were treated in ammonia gas and analyzed by MALDI mass spectrometry. The spectrum was not calibrated.

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