Figure 1 | Scientific Reports

Figure 1

From: The serine proteinase hepsin is an activator of pro-matrix metalloproteinases: molecular mechanisms and implications for extracellular matrix turnover

Figure 1

Hepsin induces cartilage degradation in OA cartilage explant cultures. (A) Hepsin or matriptase (100 nM) was added to human OA knee cartilage in explant culture. Cartilage from donor #1 (85 y, M) and #2 (77 y, F) were incubated for 3 days whilst donor #3 (66 y, F) was incubated for 7 days. (B) Hepsin or matriptase were added to human OA cartilage in the presence or absence of the metalloproteinase inhibitor GM6001 (10 µM). Collagen and aggrecan release were measured by hydroxyproline and DMMB assays, respectively. Statistical analyses were performed using one-way ANOVA with Bonferroni post-hoc test where: *p < 0.05, ***p < 0.001 versus hepsin; and ††p < 0.01, †††p < 0.001 versus control; ###p < 0.001 versus hepsin or matriptase only. Data are plotted as mean ± SD and representative of at least 3 independent experiments (n = 4).