Figure 4 | Scientific Reports

Figure 4

From: Improved Detection of Cytokines Produced by Invariant NKT Cells

Figure 4

Removal of dead cells allowed for an improved detection of iNKT cell cytokines in vitro. C57BL/6 splenocytes were either left untreated (control) or purified via a density-gradient (+Lymphoprep) and stimulated in vitro for 5 h with 100ng/ml αGalCer in the presence of the Golgi-transport inhibitors Brefeldin A and monensin. The expression of the indicated cytokines by splenic iNKT cells was analyzed by ICCS. A summary graph (left panels) and representative data (right panels) are shown. Representative data from one of at least three independent experiments are shown.

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