Number of attached cells (A), cell projected area (B), and cell circularity (C) on the FLAT (first column of graphs), FINE (second column of graphs), and COARSE (third column of graphs) surfaces with or without biomolecule coating. Cell projected area has been normalized to the uncoated condition in each graph (FLAT, FINE, and COARSE, respectively). Number of attached cells was not affected by the biomolecules on FLAT and FINE, while more cells adhered to the biomolecule-coated COARSE substrates. Cell area was significantly increased in the presence of the biomolecule on all three topographies. While low circularity was observed on all FLAT conditions, the biomolecule-coated FINE and COARSE surfaces presented lower circularity compared to the respective uncoated topography. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 vs. uncoated condition (FLAT, FINE and COARSE, respectively).