SNX27 links DGKζ to the control of transcriptional and metabolic programs in T lymphocytes

Sorting nexin 27 (SNX27) recycles PSD-95, Dlg1, ZO-1 (PDZ) domain-interacting membrane proteins and is essential to sustain adequate brain functions. Here we define a fundamental SNX27 function in T lymphocytes controlling antigen-induced transcriptional activation and metabolic reprogramming. SNX27 limits the activation of diacylglycerol (DAG)-based signals through its high affinity PDZ-interacting cargo DAG kinase ζ (DGKζ). SNX27 silencing in human T cells enhanced T cell receptor (TCR)-stimulated activator protein 1 (AP-1)- and nuclear factor κB (NF-κB)-mediated transcription. Transcription did not increase upon DGKζ silencing, suggesting that DGKζ function is dependent on SNX27. The enhanced transcriptional activation in SNX27-silenced cells contrasted with defective activation of the mammalian target of rapamycin (mTOR) pathway. The analysis of Snx27 −/− mice supported a role for SNX27 in the control of T cell growth. This study broadens our understanding of SNX27 as an integrator of lipid-based signals with the control of transcription and metabolic pathways.

both in T cell cultures and genetically modified Snx27 −/− mice. Our results demonstrate that SNX27-silencing in Jurkat T cells results in Ras/ERK/AP-1 and NF-κB pathway hyperactivation, the two main DAG-regulated pathways in T lymphocytes. Such activation did not increase further upon DGKζ silencing suggesting that constitutive interaction of SNX27 with DGKζ sustains its function as a negative regulator of DAG metabolism. Analysis of SNX27-deficient mice demonstrated normal T cell differentiation and enhanced CD69 activation in agreement with SNX27 and DGKζ acting on the Ras/ERK/AP-1 pathway. In contrast, SNX27 silencing in Jurkat cells decreased mTORC1-dependent S6 kinase (S6K) activation that correlated with limited antigen-induced cell growth of T cells from Snx27 −/− mice. Our results extend the knowledge on SNX27 functions and suggest a previous unrecognized contribution on the coordinated regulation of DAG-based signals.

Results
Analysis of DAG-regulated signaling in SNX27-silenced activated T cells. In the Jurkat T cells model, DGKζ silencing promotes PKCα/RasGRP1 activation that, upon antigenic triggering, mediates upregulation of the Ras/ERK axis 13 . We investigated the effect of SNX27 in the magnitude of Ras activation following T cell receptor (TCR) triggering. Activation of the Ras pathway can be monitored by measuring surface abundance of CD69, a C-type lectin that is transcriptionally upregulated in response to Ras/ERK activation after TCR stimulation 21 . SNX27-silenced Jurkat cells showed a significantly higher increase in the geometric mean fluorescence intensity (GMFI) of the CD69 hi population after T cell stimulation and costimulation (Fig. 1A).
Enhanced Ras/ERK signalling in DGKζ deficient T cells results in a greater proportion of CD69 expressing cells and prolonged expression of this activation marker 22 . When compared to WT mice, the analysis of long term CD69 induction in Snx27 −/− T cells demonstrated a similar effect with higher percentages of CD69 + cells both in the CD4 (Fig. 1B) and CD8 populations (Fig. 1C). This is reminiscent of the larger proportion of CD69 + T cells observed at all antigen concentrations after stimulation of Dgkzζ −/− mouse T cells 22 , and suggests that SNX27, as DGKζ, also limits the Ras/ERK signaling threshold for activation in primary mouse T cells. The GMFI of the CD69 + cells was similar in WT and Snx27 −/− cells (not shown), again resembling that described for ERK phosphorylation in Dgkz −/− mice 23 .
CD69 upregulation is largely due to AP-1 mediated transcription in response to activation of the Ras/ERK pathway. Analysis of the promoter activity of the AP-1-binding site in the CD69 promoter showed higher activity in SNX27-silenced compared to control Jurkat cells; CD28 costimulation markedly augmented this difference (Fig. 1D), which correlated well with the effect observed on CD69 induction in Jurkat and mouse T cells. To discriminate between inputs operating on ERK to CD69 transcriptional upregulation as the result of SNX27 silencing, cells were pretreated with the classic PKC (cPKC)-selective inhibitor Gö6976 (Gö) or PD98059 for MEK (MAPK/ERK kinase) before stimulation. In all cases, SNX27 depletion slightly increased AP-1 transcription independently of pharmacological inhibition of PKCα or MEK that decreased AP-1 dependent transcription to a similar extent in SNX27-silenced cells and controls (Fig. 1D).
Similar to that described for DGKζ deficiency, SNX27-silenced Jurkat T cells and SNX27-deficient splenocytes showed hyperactivation of DAG/PKC/Ras/ERK pathway, determined by the increased expression of the CD69 activation marker. To examine in more detail DGKζ contribution in SNX27-silenced cells, we compared CD69 expression in cells independently silenced for either SNX27 or DGKζ with cells silenced for the two proteins (Fig. 1E). CD69 induction upon CD3 or CD3/CD28 stimulation increased to a similar extent in double silenced cells when compared to silencing of SNX27 or DGKζ individually (Fig. 1F). The combined down-modulation of the two proteins did not have any appreciable additional effect in the presence of inhibitors (Fig. 1F).
In addition to PKCα dependent activation of the RasGRP1/Ras/ERK/AP-1 pathways, DGKζ silencing also enhances PKCθ activity downstream of CD28 19 . PKCθ triggering not only promotes ERK-dependent AP-1 transcription, but also activates the NF-κB-mediated transcription program 15 . Analyses of the promoter activity of the NF-κB binding site in the CD69 promoter confirmed increased activation in SNX27-silenced cells that paralleled the one observed upon DGKζ silencing (Fig. 1G). Dual silencing of the two proteins did not induced additional effects, supporting the idea that down-regulation of NF-κB activity by DGKζ is dependent on SNX27 expression. SNX27 regulates the PKCα/DGKζ axis. The best-known function of SNX27 interaction with its transmembrane cargoes is to facilitate their recycling to the cell surface, protecting them from degradation 5 . As a result, SNX27 silencing is reported to accelerate lysosomal dependent degradation of its partners. We reasoned that the similar effects observed upon SNX27 or DGKζ silencing could be interpreted as the result of DGKζ degradation. We thus examined DGKζ stability in SNX27 silenced Jurkat T cells. As a control, we monitored the abundance of the transmembrane proteins GLUT1 and Kidins220, both known transmembrane SNX27 PDZ cargoes 5 . Western blot analyses of DGKζ in SNX27-silenced Jurkat T cells showed no substantial changes in the protein levels of the short isoform and only modest variations in the abundance of the long isoform ( Fig. 2A,B). SNX27 silencing revealed increased levels of Kidins220 and GLUT-1 ( Fig. 2A). To assess if increased protein expression was the result of protein synthesis we examined the effect of inhibition of protein synthesis by cycloheximide (CHX). CHX treatment had no obvious effect in control cells but acutely decreased GLUT1 and Kidins220 abundance in SNX27-silenced cells ( Fig. 2A), suggesting that impaired recycling of SNX27 cargoes in Jurkat T cells is compensated by enhanced protein synthesis. Diminished GLUT1 and Kidins220 abundance in CHX treated cells was not recovered by inhibitors of the lysosome pathway (not shown) and partially recovered by proteasome inhibition. DGKζ did not behave as one of these classical SNX27 PDZ cargoes, suggesting that DGKζ stability is independent of SNX27 interaction. The best-characterized DGKζ function is that of restricting PKC activity through DAG consumption 19 . The impact of SNX27 silencing on basal PKC activity was assayed using an antibody that recognizes phosphorylated PKC substrates. Basal phosphorylation of PKC substrates increased upon SNX27 silencing, and was sensitive to Gö6976 (Gö) (Fig. 2C) suggesting increased activity of cPKCs in the absence of SNX27.
Scientific RepoRts | 7: 16361 | DOI:10.1038/s41598-017-16370-w Analysis of SNX27 deficiency on T cell development. The previous experiments indicated that, in the absence of SNX27, DGKζ cannot fulfill its role as a negative regulator of DAG-triggered signals. DGKζ-deficient mice show peripheral T cells with hyperactive responses to TCR stimulation but normal T cell development 22 . To further examine SNX27 contribution to the regulation of DGKζ functions, we characterized T cell development ) with soluble anti-CD3 alone or with anti-CD28 for costimulation. Cells were stained for CD69 surface marker and, using flow cytometry, CD69 hi cells were gated, and geometric mean fluorescence intensity (GMFI) of CD69 hi cells was calculated. (B,C) Splenocytes from WT and Snx27 −/− littermate pairs were stimulated (48 h) with plate-bound anti-CD3 alone or with soluble anti-CD28 for costimulation and stained for CD69 surface marker. The percentage of CD69 + cells were gated in CD4 + (B) or CD8 + (C) cells. Data are shown as mean ± SEM (ns no significant, p > 0.05; *p < 0.05; paired t-test; n = 4). Luciferase assays were performed to calculate (D) AP-1 and (G) NF-κB promoter activity. RLU, relative luciferase units (see Methods). Where indicated, cells were pretreated with Gö6976 or PD98059 for classic PKC or MEK inhibition, respectively. (E) Western blot analysis of cell lysates from shControl and shSNX27/DGKζ Jurkat T cells (F) shControl and shSNX27/DGKζ Jurkat T cells were treated as in A with or without inhibitors where indicated. Two-way ANOVA with the Bonferroni post-hoc test was used for multiple comparisons, both with GraphPad Prism 5 software. (ns) when p > 0.05, (*) when p < 0.05, (**) when p < 0.01, (***) when p < 0.001. in SNX27 knockout mice (Snx27 −/− ). Snx27 −/− mice were originally reported to die at 4 weeks post-partum due to postnatal growth defects 24 . Optimization of housing and feeding conditions allowed us to analyze mice from 6 to 12 weeks old. Crossing Snx27 +/− heterozygotes on C57BL/6 and 129SV mixed backgrounds 24 generated F1 hybrid background Snx27 +/+ and Snx27 −/− mice and enabled paired analysis of littermates.
Analysis of total cellularity of the lymphoid organs: thymus, spleen and lymph nodes (LN) in Snx27 −/− mice showed no defect in the appearance or cellularity of thymus and LN (Fig. 3A). Consistent with previous reports showing that Snx27 deletion in mice results in smaller animals with reduced organ size 24 , Snx27 −/− mice showed a significant decrease in spleen size (Fig. 3B) that correlated with reduced cellularity compared to wild type (WT) littermate controls (Fig. 3A). In the thymus, thymocytes develop from the CD4 − CD8 − double negative cell population (DN) to the CD4 + CD8 + double positive (DP) stage and then to the single positive (SP) CD4 + and CD8 + mature T cells. To evaluate the development of Snx27 −/− T cells, we stained thymocytes for cell surface expression of CD4 and CD8 and analyzed the different populations by flow cytometry. As described for DGKζ deficient mice 22 , the analysis of DP and SP thymocyte populations showed no significant differences (Fig. 3C,D).

Analysis of SNX27 deficiency on T cell homing. Mature T lymphocytes exit the thymus and migrate to
peripheral lymphoid organs where mature B cells also reside 25 . Albeit the spleen showed reduced cellularity, in agreement with its reduced size, quantification did not reveal major differences in the percentages of B and T cell populations in spleen (Fig. 4A,B) nor lymph nodes (Fig. 4E,F). Snx27 −/− mice showed no gross differences with WT littermates in the percentages of CD4 and CD8 cells gated in TCRαβ + (Fig. 4C,D,G,H). Overall the results indicate that Snx27 deletion in mice, as that of Dgkz, did not markedly alter thymic T cell development. In spite of their reduced size, Snx27 −/− mice nonetheless maintained peripheral T cell populations similar to WT littermates.

SNX27 deficiency promotes PKC-regulated functions but impairs activation of the AKT/mTOR axis. CD69 upregulation is a very sensitive but transient measure of antigen recognition. Expression of CD44
is also induced after antigen recognition, and remains high on all antigen-experienced cells 26 . At difference from that observed in WT splenocytes, the analysis of the CD44 + T cell population in Snx27 −/− cells revealed no substantial increase following CD3/CD28 costimulation ( Fig. 5A and B). The quantification of paired littermates showed significantly lower GFMI in CD4 T cells (Fig. 5C). CD44 GMFI was slightly reduced in Snx27 −/− activated CD8 T cells in response to either anti-CD3 or CD3/CD28 costimulation (Fig. 5D).
The analysis of CD69 induction correlated with Snx27 −/− mice displaying a larger population of responsive T cells, whereas low cell surface CD44 abundance suggested impairment in the ability to achieve a full activation phenotype. To better clarify the contribution of SNX27 to the correct activation of signals, we made use of the Jurkat T cell model. Analysis of SNX27 silenced cells revealed enhanced PKD phosphorylation, a dual, direct and PKCθ-mediated DAG effector that is very sensitive to DGKζ silencing 19 (Fig. 5E). Enhanced PKCθ activation as the result of DGKζ deficiency also promotes TCR/CD28 dependent phosphorylation and degradation of the NF-κB inhibitor IκB 19 . In accordance, SNX27 silencing led to increased IκB degradation compared to controls (Fig. 5E).
CD28 engagement also activates phosphatidylinositol (PtdIns) 3-kinase (PI3K), that favours PtdIns-dependent kinase 1 (PDK-1) and AKT recruitment and activation. This pathway activates the metabolic regulator mammalian target of rapamycin (mTOR)/S6 kinase (S6K) pathway (reviewed in 27,28 ). DGKζ silencing facilitates the association of PKCθ and PDK1 and enhances mTOR/S6K pathway activation downstream CD28 19 . In contrast with PKCθ enhanced functions, SNX27-silenced cells showed reduced mTOR activation determined by S6K phosphorylation at its Thr389 residue (Fig. 5E). Analysis of AKT confirmed a decrease in the initial phosphorylation at Ser 308, the PDK-1 phosphorylation site (Fig. 5F). In agreement with the data from SNX27 silenced Jurkat T cells, phosphorylation of the S6K effector rps6 (ribosomal protein S6) at Ser235/6 was delayed in Snx27 −/− splenocytes (Fig. 5G). AKT Ser473 phosphorylation was also reduced in Snx27 −/− splenocytes at later times, which indicated alterations in mTORC2 pathway downstream of TCR triggering. Differential effect of SNX27 and DGKζ silencing on IL-2 production. The analysis of SNX27 silenced Jurkat cells correlates with that observed in SNX27 deficient T cells and suggest that, differently from DGKζ, SNX27 is necessary for the correct activation of the AKT/mTOR/S6K pathway downstream of CD3/CD28 triggering. The integration of PKCθ and AKT signals downstream TCR and CD28 are indispensable to sustain IL-2 production 29 . The enhanced activation of PKCθ and AKT pathways in DGKζ null cells heightens IL-2 production both in genetically modified 22 and silenced activated T cells 19 . We next determined the effect of SNX27 silencing on IL-2 production and compared it with that observed upon DGKζ silencing. IL-2 production in Jurkat T cells was exclusively detected in the presence of costimulation (Fig. 6A). SNX27 silencing resulted in minor increase on IL-2 production in sharp contrast with the enhanced synthesis observed in DGKζ-silenced cells (Fig. 6A). Double silencing of DGKζ and SNX27 in Jurkat T cells resulted in further increase on IL-2 production upon costimulation (Fig. 6A). These results suggest that, in contrast to their partnership in DAG-dependent transcriptional control, DGKζ limits IL-2 production independently of SNX27 interaction. A similar analyses of IL-2 production in SNX27-deficient T cells showed diminished IL-2 production upon activation (Fig. 6B), confirming a differential effect with that described for DGKζ deficient mice. Recognition of antigens by naïve T lymphocytes triggers an activation program that induces growth and ultimately cell division. Enhanced IL-2 production in DGKζ deficient T cells correlates with hyperproliferation of naïve T cells in response to antigenic challenge 22 . We monitored cell proliferation in SNX27-defficient T cells. Before stimulation, WT and Snx27 −/− splenocytes were stained with CellTrace and at 48 h after stimulation,  stained for CD4 and CD8 expression and analysed by flow cytometry. Proliferating cells were identified by dye dilution. Compared to WT littermates, CD4 and CD8 populations from Snx27 −/− mice proliferated normally in response to CD3 stimulation and showed a slight delay in their division upon co-stimulation (Fig. 6C,D). To determine whether Snx27 deficiency induced some intrinsic defect that prevented a high division index, we used pharmacological, more potent proliferative stimuli. Cell treatments with either phorbol 12-myristate 13-acetate (PMA) plus ionomycin or concavalin A (Con A) resulted in similar numbers of dividing cells in WT and Snx27 −/− T cells (Fig. 6C and D).

SNX27 silencing limits Transferrin receptor expression.
Our experiments suggested that impaired activation of the AKT/mTOR/S6K pathway in Snx27 −/− T lymphocytes did not grossly affected cell proliferation. In mature peripheral T cells the extension of activation of the AKT/mTOR/S6K axis controls protein synthesis, nutrient uptake and cell growth of antigen-activated T cells 30,31 . Optimal growth responses in lymphocytes are associated with the upregulation of transferrin receptors that deliver iron, a necessary co-factor for several metabolic reactions, to the cell 32 . The levels of expression of CD71 (the transferrin receptor; TfR) is markedly upregulated upon costimulation and reflect the amplitude of mTOR activation 32 . Analysis of CD71 surface expression in WT splenocytes confirmed enhanced expression following costimulation. Snx27 −/− T cells upregulated CD71, although it was less abundant than in WT controls (Fig. 7A-D).
Jurkat T cells constitutively express TfR so we determined if SNX27 silencing had any effect on receptor expression. Western blot analysis of SNX27-silenced Jurkat T cells showed decreased TfR expression. In contrast to that observed for GLUT1, TfR abundance remained constant after CHX treatment, suggesting a defect independent on receptor recycling (Fig. 7E). Flow cytometry analysis confirmed reduced TfR/CD71 cell surface expression after SNX27 silencing with no significant differences between control and SNX27-silenced cells after CHX treatment (Fig. 7F).

SNX27 is required for TCR/CD28-dependent induction of T cell growth. In recently activated T lym-
phocytes, cell size can be used as a surrogate indicator of mTOR activity 33 . In cancer T cells, expression of CD44 has been linked to enhanced mTOR activation as the result of RasGRP1 mutations 34 . We examined cell surface CD44 abundance relative to T cell size following stimulation. We calculated the CD44 GMFI of cells from four distinctly sized populations [from the smallest FSC MFI population ("size 1"; S1) to the largest ("size 4"; S4) (Fig. 8A,B), and used cell surface TCRβ expression as a control. Our results showed a constant value for TCRβ GMFI and isotype controls independently of cell size whereas CD44 GMFI was directly proportional to FSC MFI (Fig. 8C,D). As a consequence, the S3 and S4 populations in CD4 and CD8 cells expressed the highest CD44 levels ( Fig. 8A and B). . (E) shControl and shSNX27 Jurkat T cells were treated with 10 μg/ml cycloheximide (CHX) for the times indicated and total levels of GLUT1, TfR, SNX27 and GAPDH as loading control were determined by western blot. Graph represents a typical experiment from three independent experiments repeated with similar results (F) shControl and shSNX27 Jurkat T cells were treated with 10 μg/ml cycloheximide (CHX) for 4 h and cell surface levels of TfR(CD71) were determined by flow cytometry. Geometric mean fluorescence intensity (GMFI) was normalized to untreated shControl cells. Data shown as mean mean ± SEM (**p < 0.01; ***p < 0.001; two-way ANOVA/Bonferroni post test) from at least three independent experiments. These data confirm that CD44 expression in recently activated T cells is a direct reporter of cell growth, and indicate that the defective expression of CD44 in Snx27 −/− T cells could be linked to impaired mTOR activation and concomitant cell growth. We next measured cell size in WT and Snx27 −/− T cells before and after activation. Naïve Snx27 −/− T cells were the same size as WT T cells (Fig. 8E,F), but they had reduced size after CD3 or CD3/ CD28 stimulation. Analysis of cell distribution among distinctly sized populations (gates shown in Fig. 8G,H), demonstrated that CD3-stimulated T cells were found mostly in the smaller S1 and S2 populations, with no gross differences between WT and Snx27 −/− T cells. CD28 costimulation resulted in a marked increase of the percentage of WT T cells distributed in the larger sized populations (S3 and S4), whereas the percentage of these populations in CD4 + and CD8 + Snx27 −/− cells remained low (Fig. 8I,J). These results correlate with previous findings and suggest that Snx27 −/− naïve T cells present a growth defect upon strong antigenic challenge.

Discussion
In this study we have explored in more depth the contribution of SNX27 to the regulation of the signals triggered by antigen recognition. Our results confirm the relevance of SNX27 interaction with DGKζ by showing that DGKζ's role as a negative regulator of DAG signals requires SNX27. We demonstrate that the effect of SNX27 silencing generally mirrors that of DGKζ depletion, suggesting that SNX27 contributes to sustain the active pool of DGKζ. SNX27 silencing impairs DGKζ function without grossly affecting protein levels, suggesting a failure for correct localization and/or function.
Our studies showing basal PKC activation in SNX27 silenced cells, suggest a failure in the crosstalk between DGKζ and PKCα. These two proteins exist in a tight equilibrium where local DAG regulation by DGKζ limits PKCα activation, whereas upon activation PKCα phosphorylates DGKζ disrupting their interaction 35,36 . Studies in other systems have suggested that the crosstalk between DGKζ and PKCα requires PDZ containing scaffolds. At neuronal synapses, the PDZ-containing scaffold protein PSD-95 (postsynaptic density protein) enables this regulatory mechanism in basal conditions 37 . In neuromuscular junctions, the PDZ proteins syntrophins facilitate PKC-dependent localization of DGKζ to junctions 38 . In T cells, where reciprocal PKCα/DGKζ modulation also occurs 13 , SNX27 acts as an endosomal scaffold for DGKζ to control basal PKC activity. We hypothesize that PKC activation, as the result of the disruption of SNX27/DGKζ association, reflects impaired DAG consumption at the endosomal/recycling compartment where SNX27 resides.
Antigenic recognition by T cells triggers rapid elevation of DAG at the contact area. The rapid recycling of DAG-enriched organelles together with local generation of DAG by PLCγ-dependent PIP2 hydrolysis helps to sustain activation of DAG effectors at this particular localization. Functional inactivation of DGKζ in recycling endosomes as a result of SNX27 silencing correlates with the enhanced activation of DAG-regulated pathways triggered by co-stimulatory signals. SNX27-silenced cells showed enhanced activation of DGKζ-regulated pathways including enhanced NF-κB and AP-1 promoter activity upon costimulation. The effects of silencing SNX27 and DGKζ on CD69 upregulation and NF-κB activity were not enhanced in double-silenced T cells, which supports the hypothesis that SNX27 controls DAG signaling through DGKζ interaction. The analysis of Snx27 −/− mice demonstrated normal thymus development and hyperactivation of DAG-mediated signals in mature T cells, similar to that described for dgkζ −/− mice. Consistent with previous reports 24 Snx27 −/− mice showed a significant decrease in spleen size in agreement with the reduced size of the animals. Reduced spleen size correlated with small number of cells although the percentage and size of T cells was maintained. In agreement with that observed in Jurkat T cells, Snx27 −/− splenocytes revealed normal activation of the Ras/ERK pathway and enhanced expression of the CD69 activation marker.
DAG regulated transcription is essential for T cells to synthesize and secrete IL-2. In contrast to the non-additive effects on NF-κB and AP-1 promoter activity observed in the luciferase reporter assays, T cells silenced for both DGKζ and SNX27 showed enhanced IL-2 production compared to those silenced for SNX27 alone. This suggests that not all of the functions attributed to DGKζ are impaired following SNX27 silencing. In agreement, SNX27 silencing partly reduced S6K activation in CD3/CD28 activated T cells, while previous reports found that DGKζ silencing led to increased CD3/CD28-dependent activation of the mTOR/S6K pathway 19 .
Impaired mTOR activation correlated with defective growth of naïve T cells in response to antiCD3/CD28 stimulation. mTOR is a major regulator of T cell metabolism and its activation promotes the expression of nutrient receptors like CD71 required to support cell growth 27 . Our studies agree with those showing that T cell size after TCR/CD28 costimulation correlates with mTOR activity 33 and demonstrate that CD44 expression, a reporter of tonic mTOR activity in T cells 34 , is also a marker of T cell growth upon activation. mTOR is a nutrient sensor activated by amino acids and glucose that, in T cells is tightly regulated by activation of the PI3K/PDK-1/AKT axis. DGKζ-silenced T cells and DGKζ deficient mice show enhanced AKT and mTOR dependent S6K activation 19 . Ectopic DGKζ overexpression impairs mTOR activation in a kinase dependent manner 39 . SNX27 silencing does not affect DGKζ expression, suggesting that DGKζ inhibitory function on the AKT/mTOR axis is enhanced in the absence of SNX27. In this regard, double SNX27 and DGKζ silencing in Jurkat T cell enhances IL-2 production. Additional SNX27 cargoes may also contribute to the differential effects observed for TCR-mediated AKT/mTOR activation. Proteomic studies and in silico analysis demonstrate that there are hundreds of potential proteins that can interact with SNX27 7 . DGKζ is a high affinity SNX27 interactor, so DGKζ silencing could favor SNX27 nteraction with other cargoes enhancing the regulation of the mTOR/S6K axis. SNX27 deficiency on the other hand would limit this function. Additional studies should explore in depth if DGKζ contributes to limit TCR-triggered mTOR activation in SNX27 deficient T cells.
SNX27-mediated control of mTOR correlates with other studies in mice where low or null expression of the proteins that participate in the mTOR signaling pathway results in small animals with reduced organ size (reviewed in 40 ). Snx27 −/− mice are indeed smaller than controls 24 . Nevertheless, while we observed decreased spleen cellularity in Snx27 −/− animals compared to controls, thymus and LN cellularity were unaltered. Normal thymus and LN cellularity concur with no gross defects in T cell development. Thymic size and thymopoiesis capacity are determined by thymic niche availability and by T cell progenitor dosage, which depends on cell proliferation and apoptosis during development and thymic involution (reviewed in 41 ). Additional studies are needed to fully explore if the lack of SNX27 affects hematopoietic cell development. It will also be of interest to examine if the minor size of SNX27 defficient mice is due to alterations in the correct activation of the AKT/mTOR/S6K pathway.
In summary our studies demonstrate that SNX27 interaction with DGKζ enables adequate metabolism of the DAG that is generated during T cell activation. In addition, SNX27 facilitates the normal growth of naïve, quiescent T cells when they have high metabolic demands. Albeit currently considered linked events, cell growth and cell cycle progression are nonetheless distinct processes in mammalian cells. Our studies corroborate this observation and agree with studies showing that, in T lymphocytes, mTOR promotes cell cycle progression but is not strictly necessary for proliferation (reviewed in 42  Leupeptin and aprotinin were purchased from Roche. We used Na 3 VO 4 , PMSF, β-glycerophosphate, paraformaldehyde (PFA), cycloheximide (CHX), concanavalin A (ConA), BSA and NP40 (all from Sigma-Aldrich). Gö6976, PD98059 and MG-132 were from Calbiochem.
Snx27 +/− mice were kindly provided by Dr. Wanjin Hong (Institute of Molecular and Cell Biology, Singapore) 24 . Mice were housed in specific pathogen-free conditions and handled in accordance with the Australian Code of Practice for the Care and Use of Animals for Scientific Purposes. All mouse strains and experimental protocols were conducted in accordance with the Animal Ethics Committee of the University of Queensland (approval #IMB/234/16/NHMRC/ARC and IMB/190/16/NHMRC/BREED).
Silencing experiments. For SNX27 and DGKζ silencing, pSUPER-derived plasmids were used as detailed in 19 .
Dual luciferase reporter assays. Assays were performed as described in 19 . Briefly, Jurkat cells were transfected with the indicated shRNA constructs. At 24 h post-transfection, cells were washed and allowed to recover (24 h), then transfected with 15 μg of the indicated promoter construct (pGL2 AP-1 or pGL4-NF-κB) and 5 μg renilla luciferase vector pRL-TK (Promega) as internal control. After 24 h, cells were washed, allowed to recover (6 h) and stimulated as above. Cells were harvested and assayed for luciferase activity using the Dual-Luciferase Reporter Assay (Promega). Luciferase activity was reported relative to renilla luciferase activity (RLU). Proliferation assays. Cell proliferation was analyzed using the Cell Trace Violet Cell Proliferation kit (Invitrogen). Cells were stained according to manufacturer's instructions, cultured for indicated times and processed for flow cytometry.
Protein Stability Assays. Jurkat T cells were treated with 10μg/ml cycloheximide (CHX) alone, or in combination with MG-132 (5 μM) and abundance of the indicated proteins was assessed by quantitative western blot. Protein levels were normalized to GAPDH or α-tubulin.
Western blot analysis. Cells were lysed in NP40 buffer (10 mM HEPES pH 7.5, 15 mM KCl, 1 mM EGTA, 1 mM EDTA, 1% NP40, 10% glycerol); clarified lysates were quantified with the Pierce 660 nm Protein Assay (Thermo Scientific). An equivalent protein amount per sample was analyzed by SDS-PAGE. Proteins were transferred to nitrocellulose membrane (Bio-Rad) and incubated with indicated primary antibodies. For HRPor fluorescent-conjugated secondary antibodies, we used an ECL detection kit (Amersham Bioscience) or an Odyssey scanner (LI-COR), respectively. Densitometric analysis of proteins in western blots was performed using ImageJ.
Flow cytometry analysis. Cells were collected in ice-cold PBS and cell surface proteins stained with saturating concentrations of the indicated fluorophore-conjugated primary antibodies in PBS staining buffer (1% FBS, 0.5% BSA, 0.01% sodium azide/PBS) (30 min, 4 °C). Cells were washed with the same buffer and fixed using 1% PFA/PBS or maintained at 4 °C for flow cytometry using Cytomics FC500 or Gallios cytometer (Beckman Coulter). Live cells were gated using forward and side scatter parameters. For fixed cells, LIVE/DEAD violet dead fixable cell stain (Invitrogen) was used. For primary cells, each sample was acquired for a minimum of 100,000 events. Data were analyzed using FlowJo software (TreeStar).
IL-2 production assays. Jurkat T cells (2.5 × 10 5 cells in 200 μl) were seeded in a flat bottom 96 well plate in triplicates, and stimulated as above, for 16 h at 37 °C. Then, ELISA test was performed on the culture supernatants, according to manufacturer's instructions (Human IL-2 ELISA MAX TM Delux, Biolegend). Primary T cells were stimulated as indicated under activation assays, after 48 hours supernatants were collected and IL-2 determined according to manufacturer's instructions (Mouse IL-2 ELISA MAX TM Delux, Biolegend)