Sphingolipids regulate critical cellular processes including inflammation. Ceramide, which serves a central role in sphingolipid metabolism, is generated by six ceramide synthases (CerS) that differ in substrate specificity. CerS6 preferentially generates C16-ceramide and its mRNA is highly expressed in immune tissues. In this study we analyzed how deficiency of CerS6 impacts on the development of colitis using an adoptive transfer model. Adoptive transfer of CerS6-deficient splenocytes, which have significantly decreased levels of C16-ceramide, showed that CerS6-deficiency protected against the development of colitis. However, adoptively transferred cells isolated from the lamina propria of the large intestine from wild type or CerS6-deficient groups showed no differences in the percentages of immune-suppressive regulatory T cells, pro-inflammatory Th17 cells, or their ability to express IL-17. In vitro polarization of wild type or CerS6-deficient splenocytes also revealed no defects in the development of T cell subsets. Our data suggest that protection from colitis following adoptive transfer of CerS6-deficient splenocytes maybe related to their ability to migrate and proliferate in vivo rather than subset development or cytokine expression.
Sphingolipids are a lipid subtype that can regulate critical processes such as cell growth and death, autophagy, migration and invasion, angiogenesis and inflammation1,2. Central to sphingolipid metabolism is ceramide, which varies in acyl chain length ranging from 14–30 (or more) carbons3. This variety in acyl chain length derives from the activity of six ceramide synthases (CerS) that have different substrate preferences4. Through the combined activity of multiple CerS, cells are endowed with a specific ceramide profile that likely reflects the need for proper signaling and sphingolipid homeostasis in any given tissue. Changes in ceramide profiles have been observed in irritable bowel syndrome, Alzheimer’s disease, cystic fibrosis, multiple sclerosis, and cancer, suggesting that dysregulation of CerS may contribute to onset or progression in a variety of diseases5,6.
The development of CerS-deficient mice has shed some light onto the roles and functions of specific ceramides and suggests that ceramide fatty acid length is associated with distinct biological roles. For example, CerS3 or CerS4-deficient mice develop problems with skin barrier function and alopecia, respectively7,8, while CerS1- and CerS2-deficient mice exhibit abnormalities in the central nervous system5. CerS2-deficiency (inability to generate C24-Cer) results in a compensatory generation of C16-Cer, which in the liver leads to apoptosis and subsequent development of hepatocellular cancer9,10. Lack of CerS5/6 expression impacts metabolism in a diet-induced obesity model and deficiency of CerS6 can also result in behavioral defects11,12,13. Altered expression of CerS2, CerS5, or CerS6 can impact cytokine production and inflammation12,14,15,16,17. As CerS6 mRNA is strongly expressed in cells of the immune system, we investigated how CerS6-deficiency impacts on the development of colitis in an adoptive transfer model4.
Characterization of CerS6-deficient mice
CerS6-deficient mice were generated on a C57BL6 background by the Texas Institute for Genomic Medicine. In our experience homozygous breeding of CerS6-deficient mice is difficult as pups often fail to thrive. Therefore all experiments were performed with wild type and CerS6-deficient littermates derived from heterozygous parents. CerS6-deficient mice tended to be significantly smaller in overall body size than their wild type littermates (Fig. 1a). However, when separated by gender the difference was only significant in males (p < 0.05, n = 5). Analysis of ceramides from lung, liver, and kidney revealed a significant decrease in C16-ceramide, which in liver and kidney was accompanied by an increase in C24:1-ceramide (Fig. 1b, Fig. S1, Table S1). Splenocytes did not differ in total ceramide content but CerS6-deficiency resulted in a significant decrease in C16-ceramide compared to wild type cells (Fig. 1c,d). This decrease in C16-ceramide did not affect the proportion of splenic CD4+ and CD8+ T cells (Fig. 1e) or memory phenotype (Fig. S2). Levels of sphingosine-1-phosphate (S1P), which can functionally oppose ceramide, were comparable (2.5 ± 0.2 vs. 2.6 ± 0.1 pmol/20 million cells in wild type and CerS6-deficient splenocytes, respectively).
Adoptive transfer of CerS6-deficient naïve CD4 cells protects against colitis
To determine whether CerS6 splenocytes differed in their ability to induce colitis, we isolated naïve CD4+CD45RBhi cells from either wild type or CerS6-deficient mice and adoptively transferred them into RAG1-deficient recipients. Three weeks after adoptive transfer mice receiving wild type but not CerS6-deficient cells experienced significant weight loss that continued until the end of the experiment (Fig. 2).
Upon euthanasia small and large intestines were collected to evaluate histology and to isolate cells from the lamina propria. Tissues were evaluated using a modified Dieleman scoring method that assessed multiple parameters as described in the Materials and Methods. In the small intestine of the wild type group, inflammatory infiltrate was evident in the muscularis and corresponded to mild or moderate inflammation. In the CerS6-deficient group the infiltrate was limited to the crypt base and was associated with mild or moderate inflammation severity. There was no increase in neutrophils and epithelial morphology was unremarkable within the small intestine of both groups. The number of lymphocytes recovered from the small intestine lamina propria was insufficient for flow cytometry analysis. Evidence of disease was more pronounced in the large intestine, where the total histological score was significantly higher in the wild type group (Fig. 3a). Inflammation was moderate to severe in the wild type group compared to generally minimal or mild in the CerS6-deficient group (Fig. 3b). In mice receiving wild type cells, extensive infiltrate reached the muscularis mucosae (with thickening of mucosa and abundant edema) or the submucosa. In contrast, in the CerS6-deficient group infiltrate tended to be limited to around crypt bases sometimes reaching the muscularis. Crypt inflammation was detected in half of the mice in the wild type group but not in the CerS6-deficient group. Crypt sloughed epithelial cells were significantly higher in the wild type compared to the CerS6-deficient group (Fig. 3c). Crypt sloughing is a pathological process that occurs when crypts become hyperplastic and epithelial cells that normally slough from the tip of the villi start sloughing into the lumen. This process may eventually lead to crypt abscesses. Epithelial morphology was also more affected following adoptive transfer of wild type rather than CerS6-deficient cells, although differences did not reach statistical significance (Fig. 3d). Adoptive transfer of wild type cells tended to result in moderate epithelial hyperplasia in the majority of mice (score 2 or 3), whereas mice receiving CerS6-deficient cells tended to have no or mild hyperplasia (score 0 or 1). Neutrophils were largely normal except for a slight increase in the colon of two mice that received wild type cells. Representative images of pathologies observed in wild type and CerS6-deficient mice are shown in Fig. 4.
Next, we isolated cells from the gut lamina propria to determine potential differences in the proportion of immunosuppressive Treg or pro-inflammatory Th17 cells. The percentage of cells expressing the transcription factors FoxP3 and RORγT that are characteristic of immunosuppressive Treg and pro-inflammatory Th17 cells, respectively was comparable between the groups (Fig. 5a,b). No differences were detected in the ability of Th17 RORγT+ cells to express IL-17 (Fig. 5c). Interestingly, while sufficient cells for flow cytometry were recovered from all mice in the wild type group (8/8), we were able to recover cells from only 3 of 5 mice in the CerS6-deficient group. This did not appear to be a failure of the cells to populate recipients following adoptive transfer as neither spleen weight (p = 0.176) nor the number of splenocytes (p = 0.158) significantly differed between CerS6-deficient and wild type groups.
Decreased C16-ceramide levels do not interfere with in vitro polarization into Treg or Th17 cells
To confirm that CerS6-deficiency does not impact T cell subset development, we also polarized cells in vitro. Splenocytes were incubated under conditions known to drive differentiation towards T1, T17 and Treg subsets. Expression of the transcription factor Tbet, which regulates the development of naïve T lymphocytes was expressed under all polarizing conditions, while RORγT and FoxP3 were preferentially expressed under condition that favor the development of pro-inflammatory T17 cells and immunosuppressive Treg cells, respectively (Fig. S3). There were no significant differences among transcription factor expression between wild type and CerS6-deficient cells (Fig. S3). Both IFN- γ and IL-17 have been linked to the development of colitis. We therefore analyzed the ability of Th1 polarized CD3+CD8+ cells to express IFN-γ and Th17 CD3+CD4+ cells to express IL-17. Stimulation with PMA/ionomycin resulted in comparable induction of cytokine expression in both wild type and CerS6-deficient cells (Fig. 6). Taken together these results suggest that the decrease in C16-ceramide resulting from CerS6 deficiency does not impact the ability of cells to develop into T cell subsets or express cytokines such as IL-17 or IFN- γ.
To evaluate whether CerS6 diminishes proliferative capacity, we activated splenocytes from wild type and CerS6-deficient mice (day 0) and counted cells on day 4 and 10. We found that CerS6-deficient cells expanded less by day 10 (Fig. 7a). To determine whether lack of CerS6 can diminishes proliferation in a non-T cell model, we evaluated SW480 colon cancer cells that express a doxycycline-inducible CerS6 shRNA18. As shown in Fig. 7b, treatment with doxycycline did not affect SW480 control cells but proliferation was reduced by approximately 25% in cells that expressed CerS6 shRNA.
Ceramide synthase 6, which preferentially generates C16-ceramide, has been implicated to play roles in various cellular responses19. CerS6-deficient mice were significantly smaller than their wild type littermates (Fig. 1a). Reduced body weight is likely due to an effect on fat metabolism as CerS6-deficient mice are protected from diet-induced obesity13. Transgenic mice that lack CerS5, which like CerS6 preferentially generates C16-ceramide, also maintain a lean phenotype on a high fat diet, although no significant difference in body weight was discernible on a low fat diet12. Our mice were fed a regular diet and therefore differences in body weight were subtle, becoming more apparent with age but clearly significant upon comparison of male littermates. Characterization of ceramide composition confirms that absence of CerS6 results in a specific decrease in C16-ceramide (Fig. 1, Table S1). In contrast to a recent study that reported a higher proportion of splenic CD8+ cells in CerS6-deficient mice, we did not observe any effect of a specific decrease in C16-ceramide on the proportions of splenic CD4+ or CD8+ T cells or on memory phenotype20. The underlying reason for this discrepancy is unclear.
Adoptive transfer of naïve CD4+ T cells resulted in significantly reduced colitis following adoptive transfer (Figs 2–4 ). These results are consistent with other recent studies in which CerS6-deficiency had a protective effect against inflammation. For example, transfer of CerS6-deficient T cells also led to reduced development of graft-vs-host disease and diminished macrophage infiltration and pro-inflammatory gene expression in the diet-induced obesity model13,20. In the graft-vs-host disease model, CerS6-deficiency interfered with allogenic T cell responses by reducing transduction of the TCR signal20. The mechanism by which CerS6-deficiency protects from inflammation in the diet-induced obesity model remains to be elucidated but was not directly mediated by macrophages as lineage-specific ablation of CerS6 failed to recapitulate the protective effect13. The pro-inflammatory lipid sphingosine-1-phosphate (S1P) has been associated with colitis and targeting S1P signaling has shown promising results in preclinical models21. However, since levels of S1P did not differ in splenocytes from wild type and CerS6-deficient mice, it is unlikely that altered S1P signaling contributed to the colitis phenotype observed in our study.
In patients with colitis, Th17 cells are increased while Treg are decreased, suggesting the proportion of these cells plays an important role in disease development22. The underlying reason for diminished development of colitis in our model does not appear to be a direct result of altered T cell subsets (i.e. more immunosuppressive Treg or less pro-inflammatory Th17 cells) or the ability to express the pro-inflammatory cytokine IL-17. Our data suggest that CerS6-deficiency may interfere with migration or proliferation in vivo rather than alter T cell subsets. In cancer cells, altered membrane fluidity in CerS6-deficient cells reduces cell migration and invasion of in vitro 23,24. Although mechanisms of motility between cancer cells and lymphocytes are different, it is possible that CerS6-deficient T cells have a reduced ability for homing and invasion of intestinal tissues. Our data suggest that CerS6-deficient splenocytes have a reduced capacity for clonal expansion. Reduced proliferation has been observed in CerS6-deficient T cells by Sofi et al.20. Expression of CerS6 shRNA also decreased the growth of SW480 cancer cells. However, loss of CerS6 does not universally decrease proliferation as targeting this enzyme results enhanced proliferation in melanoma cells20,25.
It is important to point out that CerS6-deficiency is not consistently protective against inflammation. In experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis, CerS6-deficiency enhances inflammation through increased infiltration of neutrophils as CerS6 was not able to counteract CD11b mediated activation and CXCR2-mediated migration11. Thus the different consequences of CerS6 ablation in various models and cell types underscores the complexity of ceramide synthase signaling, which may depend on the overall sphingolipid environment in which ceramide synthase isoforms are modulated. Ceramide synthases differ not only in tissue distribution but also in subcellular localization and their ability to form homo- or heterodimers4,19,26. CerS2, -4, -5 and -6 activity can also be modulated through phosphorylation27. Thus regulation of CerS activity is multi-faceted and additional studies to understand the biology of ceramide synthases will be required before therapeutic strategies to modulate the activity of ceramide synthases can be rationally designed.
All animal experiments were performed with approval by the Institutional Animal Care and Use Committee at the Medical University of South Carolina to ensure that ethical regulatory and policy mandates governing the use of animals in research are met (Animal Welfare Assurance #A3428-01). All methods were performed in accordance with the relevant guidelines and regulations. CerS6-deficient mice (C57BL6) were generated by the Texas Institute of Genomic Medicine and obtained from Dr. Besim Ogretmen. Colonies were maintained through heterozygous breeding. Genomic tail DNA was used to genotype offspring by PCR. Primers were as follows: 5′TTCGGTTAAGAATGGCCTTG3′; 5′CACACCCATATGGAACTCTTACA-3′; and 5′-CCAATAAACCCTCTTGCAGTTGC-3′. Expected PCR products are 460 bp for wild type, 295 bp for CerS6 knockout, or both for heterozygous animals. RAG1-deficient mice (B6.129S7-Rag1tm1Mom/J) were purchased from Jackson Laboratories.
Ceramide species (C14, C16, C18, C18:1, C20, C20:1, C20:4, C22, C22:1, C24, C24:1, C26 and C26:1 were quantified by LC/MS as previously described18.
Cell isolation, polarization, and adoptive transfer
Four million wild-type or CerS6-deficient splenocytes/well in a 24-well plate were polarized in 2 ml RPMI with 10% FBS (Hyclone) and 1 μg/ml anti-mouse CD3 as follows: For T1 polarization, 6ng/ml mIL-12, 20 μg/ml anti–mouse anti–mouse IL-4 (anti-mIL-4, clone 11B11), and 200 IU/ml hIL-2 were added. For T17 polarization 100 ng/ml recombinant human (rh) IL-6 (NIH repository), 100 ng/ml rhIL-21 (Shenandoah), 30 ng/ml rhTGF-β1 (Biolegend), 10 ng/ml rhIL-1β (NIH Repository), 10 μg/ml each of anti–mIFN-γ (clone XMG1.2), anti–mIL-4 (clone 11B11), and anti–mIL-2 (clone JES6-1A12) (all BioXCell) were added. For Treg we added 2 μM rapamycin, 60 ng/ml rhTGF-β1 (Biolegend), and 200 IU/ml IL-2. On day 3, 1 ml of the supernatant was removed without disturbing the cells and 20 ng/ml IL-23 and 50 IU/ml IL-2 or 200 IU/ml IL2 were added to Th17 and Treg cultures, respectively. On day 4 cells were counted and placed into fresh RPMI with 10% FBS and 100 IU/ml IL2. Flow cytometry was performed 3, 5- or 9 days after polarization.
For adoptive transfer, splenocytes from wild type or CerS6 deficient mice were pooled and naïve CD4+ mouse splenocytes obtained through deletion of non-target cells (Naïve CD4+ T cells isolation kit, Miltenyi Biotec 130-104-453) followed by further selection for CD45RBHI cells (Biolegend) as previously described28. Prior to adoptive transfer, flow cytometry was used to verify that populations contained at least 95% of desired cells. 200,000 cells/animal were injected into RAG-1-deficient recipients via retro-orbital route. Data in this manuscript were from 2 independent experiments performed with a total of 10 wild type and 8 CerS6-deficient cell recipients.
Isolation of lamina propria cells was performed as previously described29. Briefly, after small and large intestines were cleaned, a small section of tissue was collected for histology and the remainder digested (2 mg/ml collagenase D, 0.1 mg/ml DNAse I and 0.075 U/ml dispase), and washed. Lymphcytes were enriched by Percoll centrifugation and cells at the interface collected for subsequent analysis.
Cells were incubated with or without PMA/ionomycin in Golgi-Stop for 4 hours and then surface stained with fluorochrome-conjugated antibodies against CD3, CD4, and CD25 to allow for gating of specific populations. For subsequent intracellular staining, surface stained cells were washed, fixed in 4% paraformaldehyde for 20 minutes, and permeabilized for 30 minutes prior to overnight incubation with antibody (FoxP3, RORγT, IL-17, IFN-γ). After washing, signals were acquired using the BD LSRFortessa cell analyzer (BD Biosciences) and analyzed with the FlowJo software (Tree Star Inc.). Treg were defined as CD3+CD4+CD25+FoxP3+ and Th17 cells as CD3+CD4+ RORγT+.
Intestinal tissues were removed and fixed in formalin. Sections were placed in cassettes and cut longitudinally for histological analysis. Tissues were scored as follows: lymphoid follicles (0, none; 1, one; 2, two; 3, three; 4, greater than 3); infiltration of inflammation (0, none; 1, around crypt bases; 2, infiltrate to muscularis; 3, extensive infiltrate reaching muscularis mucosae with thickening of the mucosa with abundant edema); epithelium (0, normal morphology; 1, loss of goblet cells; 2, loss of goblet cells in a large area; 3, loss of crypts; 4, loss of crypts in a large area/polypoid formation); depth of ulcer (0, none; 1, mucocal involvement; 2, mucosal, submucosal; 3, penetration of muscularis propria; 4, full thickness); extent of ulcer (0, none; 1, punctate; 2, minimal; 3, moderate; 4, widespread); crypt sloughed cells (0, none; 1, 1 cell; 2, 2–5 cells; 3, many); inflammation severity (0, none; 1, minimal; 2, mild; 3, moderate; 4, marked – severe)30,31,32.
Paired (Fig. 1a) or two-sample t-tests (Figs 1b,d and 3) were used to compare means between wild type and CerS6-deficient groups. Statistical significance was defined by a two-sided alpha level of 0.05. In Fig. 2, relative body weight (weight divided by baseline weight) was modeled over time using linear regression, estimated using generalized estimating equations to account for repeated measures over time. Based on exploratory data analysis, there appeared no need to transform the outcome measure. The fitted model included treatment effects, a linear term for time, and also a quadratic time term to account for the non-linear pattern over time, and interactions between time main effect and treatment groups. Linear combinations of model coefficients were used to evaluate differences between groups at individual time points, using Wald tests and 95% confidence intervals.
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The authors would like to thank the Hollings Cancer Center shared resources facilities for assistance with lipidomics analysis, flow cytometry, and biostatistics. Shared Resources of the Hollings Cancer Center, Medical University of South Carolina were in part supported by P30 CA138313. We would also like to thank Margaret Romano at the Pathology Core Facility for preparation of fixed tissue for histological analysis. The research presented in this article was supported in part by pilot research funding from the COBRE in Lipidomics and Pathobiology grant (5P30GM103339-03) and a Program Project grant (P01CA203628) at the Medical University of South Carolina.
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Scientific Reports (2018)