Figure 1 | Scientific Reports

Figure 1

From: Frequency of Human CD45+ Target Cells is a Key Determinant of Intravaginal HIV-1 Infection in Humanized Mice

Figure 1

Greater than 10% human CD45+ cells in the peripheral blood is associated with higher susceptibility to HIV-1 transmission. Hu-mice with <10% circulating hCD45+ cells (AD) and >10% circulating hCD45+ cells (E–H), identified by flow cytometry, were challenged IVAG with low (103) (ACEG) or high (105 TCID50 (NL4.3-BAL)) (BDFH) dose HIV-1. Paired viral titres (RNA copies/mL) were quantified in the vaginal lavage (blue) and plasma (red) of Hu-mice by clinical real-time RT-PCR. Infection did not occur in Hu-mice with <10% hCD45+ cells in the peripheral blood when challenged with low dose HIV-1 (AC – 1/6 infected) while those challenged at high dose were more likely to be infected (BD – 8/12 infected). Several Hu-mice with >10% hCD45+ cells in the peripheral blood became infected following IVAG challenge with both low (EG – 4/5 infected) and high dose (FH – 10/12 infected) HIV-1. Each symbol represents an individual Hu-mouse and paired titres are denoted by identical symbols on the corresponding graph (ie. A and CB and DE and GF and H). Data was pooled from >3 independent experiments. One experiment did not include vaginal and plasma titres at week 1, thus some values for week 1 titres were omitted. IVAG: intravaginal.

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