Figure 2: | Scientific Reports

Figure 2

From: Activation of Fas/FasL pathway and the role of c-FLIP in primary culture of human cholangiocarcinoma cells

Figure 2

Apoptosis and proliferation in mixed and mucin-iCCA cells. (A) Representative phase contrast images of mixed- and mucin-iCCA cells cultured alone and co-cultured with PBMCs. (B) Western Blot analysis of PCNA and NF-kB p65 in mixed- and mucin-iCCA cells cultured alone and after co-culture with PBMCs. Actin bands represent a control of protein loading; histograms shown as mean ± SD, **P < 0.01, *P < 0.05, versus iCCA cells cultured alone at the same time points. (C) Immunofluorescence and pseudocolor analysis of PCNA immunolabeling in mixed- and mucin-iCCA cells cultured alone and after 24 h of co-culture with PBMCs. Scale bar 50 µm. (D) Histograms show the mean ± SD of cancer cell counts obtained from both iCCA cells cultured alone and co-cultured with PBMCs at different time points, *P < 0.05, versus iCCA cells cultured alone at the same time points. (E) Representative FACS analysis of Propidium Iodide and Annexin V double stained mixed- and mucin-iCCA cells cultured alone and co-cultured with PBMCs at different time points. Statistical analysis was carried out by Student t-test. Full-length blots in (B) are presented in Supplementary Figure S3.