Evaluation of Fas and FasL in intrahepatic cholangiocarcinoma (iCCA) cells. (A) Western Blot analysis of Fas and FasL performed on iCCA cells cultured alone and after 24, 48 and 72 h of co-culture with PBMCs; histograms represent mean ± SD; *P < 0.05, **P < 0.01, ***P < 0.001; §P < 0.05 iCCA co-cultured with PBMCs versus iCCA cells cultured alone at the same time points. Statistical analysis was carried out by Student t-test. (B) Confocal images of iCCA cells labelled by anti-FasL and by anti-Fas Abs. Cells were counterstained by DAPI. The squares in the images indicate magnification area shown on the right side. Scale bar = 10 µm. Full-length blots in (A) are presented in Supplementary Figure S2.