Heterogeneity of tumor tissues. (a) The workflow shows the rapid dissociation of cells from two sites of a primary tumor to generate single cells and sphere cells. The obtained cells were then analyzed by Nx1-seq and by histopathological analysis. (b) Clustering analysis. t-SNE map representation of transcriptome similarities between individual cells. The upper two panels represent T cells (red) from the E-side and the M-side, while the lower two panels represent macrophages (red) from each side. (c) Immunohistostaining of endometrial adenocarcinoma at the myoinvasive state. The endometrial side and myoinvasive tumor side were stained using specific antibodies. (d) Heterogeneity of cancer cells. EA, EAintEMT and EAEMT cells were defined as described in the Materials and Methods. *p < 0.05 for the E-side versus M-side by the Mann–Whitney U-test. (e) Cells infiltrating into tumor tissues. These cells were defined as described in the Materials and Methods. *p < 0.05 for the E-side versus M-side by the the Mann–Whitney U-test. (f) Chemokine production in cancer cells and infiltrating immune cells in each side. (g) Comparison of CCL4 and CCL20 expressing cells between the E-side and the M-side. These data were calculated using cells extracted from defined cell data shown in Supplementary Table 2. ***p < 0.001 for the E-side versus M-side by the Mann–Whitney U-test.