Figure 4 | Scientific Reports

Figure 4

From: Correlating Fluorescence and High-Resolution Scanning Electron Microscopy (HRSEM) for the study of GABAA receptor clustering induced by inhibitory synaptic plasticity

Figure 4

Post-synaptic surface and post-synaptic GABAARα1 distribution at inhibitory synapses of CTRL and NMDA stimulated hippocampal neurons. (a): 3D model of an inhibitory synapse immuno-labelled for the GABAARα1 in a hippocampal neuron in control conditions (CTRL). Slice1 and slice2 are two tomographic slices through the dotted lines in a. Arrowheads point to gold clusters; (b): 3D model of an inhibitory synapse immunolabelled for the GABAARα1 in a hippocampal neuron after the induction of plasticity by NMDA stimulation. Slice1 and slice2 are two tomographic slices through the dotted lines in b. Arrowheads point to gold clusters; note that in NMDA treated samples, more gold clusters are visible. (c): bar plot showing the area of the post-synaptic membrane on GABAARα1 immunolabelled synapses in both CTRL and NMDA stimulated neurons; (d): bar plot showing the number of gold clusters/synapse in CTRL and NMDA stimulated neurons; (e): bar plot showing the gold clusters volume normalized over the post-synaptic area in CTRL and NMDA stimulated neurons. Values are mean ± s.e.m. * Indicates significant differences (p < 0.01, Student’s t-test); ns = statistically not significant. Colour codes for the 3D models: post-synaptic membrane (blue), pre-synaptic membrane (green), gold clusters (yellow), and neurotransmitter vesicles (cyan). Scale bars: 200 nm.

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