MCMs improve reverse transfection of hESCs. (A) Merged FITC and phase-contrast micrographs of reverse transfection in human embryonic stem cells (H1 hESCs) in microwells. (B) Quantification of percent EGFP+ area of reverse-transfected H1 hESC colonies. (C) Influence of MCMs on pluripotency markers Oct4 and Nanog after 48 hrs of culture. (D) Mean EGFP expression of EGFP+ colony fraction, as measured by fluorescence intensity. Scale bar = 500 µm. *p-value < 0.05.