Expression analysis of miR-16 and miR-103 and HTR4b and HTR4b_2 in the human jejunal mucosa in IBS-D vs. healthy controls (Ctrl.). (A) qPCR analysis of miR-16 and miR-103. Fold-change value is based on the ratio of target miRNA and the average of reference genes normalised to the average of the healthy group. Values are means ± SEM. (14 IBS-D; 17 controls). **p < 0.01. Unpaired t-test with Welch’s correction. (B) nCounter analyses of HTR4b and HTR4b_2. Fold-change is based on the ratio between target mRNA and the average of the reference genes normalised to the average of the healthy control group. Values are means ± SEM. (30 IBS-D; 18 controls). Mann-Whitney U test. (C) Correlations of IBS symptoms with miRNA expression in the jejunal mucosa of IBS-D patients and controls. Spearman’s correlation rho was applied to the pooled data. Identical values refer to multiple equal correlation values of different individuals. n = 31 (14 IBS-D; 17 controls). Spearman’s rho (rs) and p-values (p) are given.