Figure 6: | Scientific Reports

Figure 6

From: miR-16 and miR-103 impact 5-HT4 receptor signalling and correlate with symptom profile in irritable bowel syndrome

Figure 6

The HTR4b_2 novel isoform carrying the c.*61 T > C SNP escapes miRNA regulation. Illustration of the HTR4b/b_2 related luciferase reporter gene expression experiments. (A) Respective HTR4b/i 3′UTRs including predicted miRNA target sites (I-III, indicated in blue) and luciferase gene reporter constructs. (A′) Relative luciferase activity in HEK293T transfected with miR-16 family members. (A″) Relative luciferase activity in Colo320 transfected with miR-103. (A′) and (A″) Particular miRNAs are both co-expressed with respective luciferase constructs (WT, c.*61 C, mut2, mut3, mut4) and related to negative control miRNA (neg. ctrl. miR). Values are means ± SEM. n = 4 (A′) and n = 3 (A″) experiments for each condition. (B) Respective HTR4b_2 3′UTR including predicted miRNA target site (I) and luciferase gene reporter constructs. (B′) Relative luciferase activity in Colo320 transfected with miR-15b/16/497 (miR-16 family) or (B″) relative luciferase activity in Colo320 transfected with miR-103. (B′) and (B″) Particular miRNAs are both co-expressed with luciferase constructs (WT, c.*61 C, mut1) or negative control miRNA (neg. ctrl. miR). Values are means ± SEM. (n = 5) (B′) and (n = 4) (B″) experiments for each condition. *p < 0.05; **p < 0.01; ***p < 0.001. One-way ANOVA with Bonferroni post-hoc test, WT (wild type), mut (mutated).