Reversible Nε-lysine acetylation has emerging as an important metabolic regulatory mechanism in microorganisms. Herein, we systematically investigated the site-specific and kinetic characterization of enzymatic (lysine acetyltransferase) and nonenzymatic acetylation (AcP-dependent or Acyl-CoA-dependent), as well as their different effect on activity of metabolic enzyme (AMP-forming acetyl-CoA synthetase, Acs). It was found that Bacillus subtilis acetyl-CoA synthetase (BsAcsA) can be acetylated in vitro either catalytically by lysine acetyltransferase BsAcuA and Ac-CoA (at low concentration), or nonenzymatically by Ac-CoA or AcP (at high concentration). Two distinct mechanisms show preference for different lysine acetylation site (enzymatic acetylation for K549 and nonenzymatic acetylation for K524), and reveal different dynamics of relative acetylation changes at these lysine sites. The results demonstrated that lysine residues on the same protein exhibit different acetylation reactivity with acetyl-phosphate and acetyl-CoA, which was determined by surface accessibility, three-dimensional microenvironment, and pKa value of lysine. Acetyl-CoA synthetase is inactivated by AcuA-catalyzed acetylation, but not by nonenzymatic acetylation.
Reversible Nε-lysine acetylation, which can change protein conformation, protein charge, protein stability, protein-protein interactions, protein-DNA binding affinity, or protein localization, is an abundant post-translational modification (PTM) in cells of all domains of life. Thousands of acetylated proteins were identified to be involved in diverse cellular processes, such as translation, transcription, and metabolism. Compelling evidence shows that protein lysine acetylation can occur via two distinct mechanisms: enzymatic and nonenzymatic acetylation (chemical acetylation). Enzymatic acetylation relies on a protein acetyltransferase (PAT) to catalyze donation of the acetyl group from acetyl-coenzyme A (Ac-CoA) to the ε-amino group of a lysine residue1,2,3. Recent studies have shown that acetyl phosphate (AcP), the high-energy intermediate of the phosphotransacetylase-acetate kinase pathway, can chemically acetylate lysine residues of protein directly4,5. Based on experimental data in Escherichia coli, nonenzymatic acetylation with AcP is more global and less specific than enzymatic acetylation, and is thought to predominate in affecting the levels of protein acetylation4. Deletion of acetate kinase, which increases intracellular AcP, increase protein lysine acetylation. In contrast, knockout of phosphotransacetylase, which converts Ac-CoA into AcP, decreases protein acetylation. This finding indicates that the intracellular AcP concentration is correlated with protein acetylation levels4. Lysine acetylation (or succinylation) has been proposed to also occur by Acyl-CoA-dependent chemical acylation, particularly within the mitochondria, in which the relatively high pH and Ac-CoA (or succinyl-CoA) concentrations would favour transferring acyl group to the deprotonated ε-amino group of lysines. Acyl-CoA-dependent nonenzymatic acylation results in substantial spontaneous mitochondrial protein lysine acetylation or succinylation6.
Two distinct mechanisms of lysine acetylation produce the same result, acetylation of ε-amino group of lysine within the target protein. Recently, the structural characterization and physiological function of two acetylation mechanisms was investigated. The previously identified bacterial acetyltransferases only recognize and acetylate the conserved motif (PXXXXGK) which is important for catalytic activity in these AMP-forming acyl-CoA synthetases, and therefore, acetylation inhibits their activity7. Mass spectrometric and crystallographic methods revealed AcP-dependent nonenzymatic acetylation to be specific: with specificity determined by the accessibility and three-dimensional microenvironment of the target acetyllysine5. Kuhn et al. found that an AcP-acetylatable lysine tended to be located near protein surfaces with microenvironment enriched for residues with positive charges, hydroxyls or amides, but often with an adjacent negatively charged residue. More recently, results from studies by Schilling et al. demonstrated that individual lysine sites from the same protein not only exhibit different susceptibilities to acetylation, but can also show markedly different acetylation rates in wild-type E. coli K-12 during the glucose time course experiments8. Nevertheless, the structural characterization and physiological function of two distinct mechanisms of lysine acetylation remain poorly understood yet.
In this work, we systematically investigated the site-specific and kinetic characterization of enzymatic and nonenzymatic acetylation (AcP-dependent or Acyl-CoA-dependent), as well as their different effect on activity of metabolic enzyme (AMP-forming acetyl-CoA synthetase, Acs). It was found that Bacillus subtilis acetyl-CoA synthetase (BsAcsA) can be acetylated in vitro either catalytically by lysine acetyltransferase BsAcuA and Ac-CoA (at low concentration), or nonenzymatically by Ac-CoA or AcP (at high concentration). Two distinct mechanisms show preference for different lysine acetylation site, and reveal different dynamics of relative acetylation changes at these lysine sites. Acetyl-CoA synthetase is inactivated by AcuA-catalyzed acetylation, but not by nonenzymatic acetylation.
Results and Discussion
Acetyl phosphate or acetyl-coenzyme A directly acetylates acetyl-coenzyme A synthetase
The best-studied acetylated protein is AMP-forming Ac-CoA synthetase (AcsA), which synthesizes Ac-CoA from acetate and CoA along with the hydrolysis of ATP to AMP. The activity of this enzyme is inhibited by Pat/AcuA-catalyzed acetylation in Salmonella enterica and B. subtilis 1,9, and is reactivated by either of the two protein deacetylases, the Zn(II)-dependent BsAcuC deacetylase and/or the sirtuin CobB10,11. The recent researches indicated that intracellular AcP plays a critical role in a chemical acetylation reaction, can function directly as an acetyl donor4,5. Previous work also showed that the abundant acetyl-CoA in mitochondria (their acetyl-CoA concentration is estimated to be 0.1-1.5 mM)12 results in substantial spontaneous mitochondrial protein lysine acetylation6,13. To determine if Ac-CoA or AcP can acetylate protein, we investigated Ac-CoA/AcP-dependent acetylation using the purified recombinant B. subtilis Ac-CoA synthetase (BsAcsA). Ac-CoA/AcP-induced acetylation on BsAcsA in a dose-dependent manner was observed by Western immunoblot analysis (Fig. 1). As shown in Fig. 1, the most obvious increases in acetylation level was first observed in the presence of concentration of AcP (1 mM) or Ac-CoA (200 μM). These concentrations are within the endogenous ranges reported for Ac-CoA, which can reach cytoplasmic concentrations of 20 to 610 μM in E. coli cells14,15, and for AcP, which is estimated to be 0.2-12 mM in E. coli cells4. The intracellular levels of Ac-CoA/AcP are comparable to the concentrations of Ac-CoA/AcP needed to acetylate proteins in vitro, indicating that Ac-CoA/AcP are able to acetylate chemically lysine residues in vivo.
The kinetic analysis of in vitro nonenzymatic acetylation of BsAcsA was also conducted. When incubated with AcP or Ac-CoA, BsAcsA became acetylated in a time-dependent manner (Fig. 2A). As shown in Fig. 2B, the results showed the Ac-CoA/AcP-dependent acetylation reaction to be slow and non-saturating, which are characteristics of non-enzymatic reactions5. Based on these results and previous works4,5,6,16, we conclude that Ac-CoA/AcP-dependent nonenzymatic acetylation can occur spontaneously depending on the concentrations of Ac-CoA/AcP (>1 mM AcP or 200 μM Ac-CoA).
AcuA acetyltransferase rapidly acetylates acetyl-coenzyme A synthetase at low concentration of Ac-CoA
Previous work showed that the acetyltransferase BsAcuA acetylates the conserved catalytic lysine (K549) of BsAcsA9. Indeed, we found that BsAcuA acetyltransferase quickly acetylates BsAcsA at low concentration of Ac-CoA (20 μM). As shown in Fig. 3, the obvious increases in overall acetylation occurred at 5-10 s. In the absence of BsAcuA, no acetylation of BsAcsA was observed at the Ac-CoA concentration of <100 μM for 6 h (Fig. 1A). The kinetic analysis of AcuA-catalyzed acetylation revealed that the reaction reached saturation at 180 s. The results demonstrated that acetyltransferase BsAcuA significantly accelerated the acetylation of BsAcsA at low concentration of Ac-CoA (can’t chemically acetylate ε-amino group of a lysine residue).
Acetyl-coenzyme A synthetase is inactivated by AcuA-catalyzed acetylation, but not by nonenzymatic acetylation
To investigate the effect of acetylation on AcsA enzyme activity, the activities of native (nonacetylated) and acetylated enzymes (AcuA-catalyzed acetylated AcsA, Ac-CoA/AcP-dependent acetylated AcsA) were determined. It was found that AcuA-catalyzed acetylation of Acs enzymes resulted in 85–90% decrease in acetyl-CoA synthetase activity compared to the nonacetylated enzymes, whereas Ac-CoA/AcP-dependent acetylation exerted no effect on AcsA enzyme activity (Fig. 4A). It has been reported that the conserved active-site K549 in conserved putative acylation motif PKTRSGK in BsAcsA was acetylated by acetyltransferase BsAcuA, and was critical for catalytic activity9. As shown in Fig. 4B, BsAcuA acetylated AcsAWT but did not acetylate AcsAK549Q, confirming that BsAcuA modified only the conserved lysine residue K549 in the active site. The mutants of K549 (AcsAK549Q, AcsAK549A, and AcsAK549R) were essentially inactive, with 10-20% of the wild-type AcsA activity (Fig. 4C). These results indicated that residue K549 can be acetylated by AcuA-catalyzed acetylation, but not by Ac-CoA/AcP-dependent nonenzymatic acetylation. The conclusion was in agreement with the results from Saccharopolyspora erythraea recently reported by our group17. In our previous work, we found that AcP was unable to acetylate conserved lysine residue K628 of S. erythraea AcsA2, whereas acetylated lysine residue K611 (possible role in binding the acetate substrate), thus resulted in a 30% decrease in acetyl-CoA synthetase activity17. No lysine residue in BsAcsA corresponding to K611 of S. erythraea AcsA2 was observed (Figure S1).
Enzymatic and nonenzymatic acetylation reveal different preference for lysine sites
To determine the acetylation sites resulted from two distinct mechanisms, the acetylated BsAcsA proteins (AcuA-catalyzed acetylated AcsA, Ac-CoA/AcP-dependent acetylated AcsA) were subjected to trypsin digestion, and the resulting peptides were analyzed by LTQ orbitrap Elite mass spectrometer. The data were shown in Table 1. Many acetylation sites were identified. Ac-CoA/AcP-dependent nonenzymatic mechanism acetylated more lysine residues than AcuA-catalyzed enzymatic mechanism. Surprisingly, acetylated site K549 was observed in Ac-CoA/AcP-dependent acetylated BsAcsA, while some other acetylated sites besides K549 were identified in AcuA-catalyzed acetylated AcsA.
To obtain a more precise understanding of two distinct lysine acetylation, we monitored dynamic changes of acetylation at each lysine residue over time by label-free, quantitative mass spectrometry. It was found that lysine on the BsAcsA protein exhibited different acetylation rates and different acetylation extent during the time course experiments (Fig. 5). Enzymatic and nonenzymatic acetylation reveal different preference for lysine sites with markedly different acetylation rates. The most obvious increase in acetylation was observed at K549 for AcuA-catalyzed reaction. The level of acetylation at K549 was two to three orders of magnitude higher than other acetylated sites (acetylation intensity 4.0 × 108 for K549, 5.0 × 106 for K524 at 5 s).
A dozen acetylated lysine residues were identified on Ac-CoA/AcP-dependent nonenzymatic acetylated AcsA. As shown in Fig. 6, each lysine was acetylated to a different extent at a different rate. Among them, K524 yielded the highest reactivity with both Ac-CoA and AcP, and revealed the most significant relative increase on acetylation. Other lysines showed low reactivity. Ac-CoA/AcP-dependent acetylation of K549 occurred nonenzymatically at a low level (reflecting the low stoichiometry of acetylation), which did not impact activity of AcsA. The mutants of K524 (AcsAK524A, AcsAK524Q, and AcsAK524R) revealed similar activity of the wild-type AcsA, which further demonstrated that acetylation of K524 did not affect the enzymatic activity of AcsA (Fig. 7A). On the other hand, low-level acetylation of nonspecific lysine sites (not target sites of AcuA) in AcuA-catalyzed reaction perhaps resulted from Ac-CoA-dependent nonenzymatic mechanism. Indeed, these acetylated sites were similar to those detected in Ac-CoA-dependent nonenzymatic acetylated AcsA (Fig. 6A). Taken together, enzymatic and nonenzymatic acetylation revealed different preference for lysine sites (enzymatic acetylation for K549 and nonenzymatic acetylation for K524). To investigate the effects of mutation at K524 and K549 in AcsA in vivo, alleles encoding B. subtilis AcsAWT, AcsAK549A or AcsAK524A were introduced into S. enterica Δacs strain18. The resulting strains were grown at 37 °C in 5 ml of the minimal acetate medium (10 mM acetate as sole carbon source). The growth was monitored in triplicate at OD600. Consistent with the activity of AcsA variants measured in vitro (Figs 4C and 7A), differences in growth behavior were observed. The strain synthesizing AcsAK549A failed to grow on 10 mM acetate as sole carbon and energy source, while strain that synthesized AcsAK524A grew as well as the AcsAWT (Fig. 7B).
Enzymatic and nonenzymatic propionylation shows similar behavior
Enzymatic and nonenzymatic propionylation was also investigated. When incubated with propionyl-CoA, BsAcsA became propionylated nonenzymatically in a dose and time-dependent manner (Fig. 8A,B). The most obvious increase in overall propionylation occurred in the presence of Pr-CoA concentration (200 μM), which far exceeds intracellular concentration of Pr-CoA 5.3 μM in E. coli 15. The result indicated that Pr-CoA-dependent nonenzymatic propionylation can’t occur spontaneously in vivo. Like acetylation, BsAcuA acetyltransferase quickly propionylated BsAcsA at low concentration of Pr-CoA (20 μM). As shown in Fig. 8C,E, the clear propionylation was observed at 30 s. The results demonstrated that acetyltransferase BsAcuA can transfer propionyl group to the ε-amino group of lysines. AcuA-catalyzed propionylation inactivated AcsA, but Pr-CoA-dependent nonenzymatic propionylation not (Fig. 8F). K524 revealed the highest reactivity in Pr-CoA-dependent nonenzymatic propionylation. Two distinct mechanisms of lysine propionylation showed similar preference for lysine sites (enzymatic mechanism for K549 and nonenzymatic mechanism for K524) as acetylation (Fig. 9). The acetylation and propionylation of the two lysine sites (K524 and K549) were identified in vivo (Table 1).
Structural analysis of the mechanism-sensitive lysine sites
To analyze the structural basis for selectivity, we investigated the linear neighboring amino acids of each acetyllysine, and relationship between the adjacent environment of a lysine within its folded structure and its acylation sensitivity to AcuA, AcP, or AcCoA. Two sequence logos were generated by compiling neighboring amino acids from position −10 to position +10 relative to Ac-CoA/AcP-dependent nonenzymatic acetylated lysine sites using two sample logo method19. As shown in Figure S2, the results revealed that to some extent acetyllysine sites tend to be position adjacent to glycine and negatively charged resides (glutamate (E) in left side and aspartate (D) in right side). However, no obvious sequence motif was observed. These appear to be consistent with the observation described by Kuhn et al.5.
The structure of BsAcsA was constructed by Swiss Model server (http://swissmodel.expasy.org/) based on crystal structure of acetyl-CoA synthetase from Salmonella enterica (PDB 1PG4)20. We mapped the locations of the mechanism-sensitive lysine sites (K524 and K549) onto BsAcsA structure, and analyzed the secondary structures in which K524 and K549 reside (Fig. 10). Structural analysis showed that the nonenzymatic acetylation-sensitive K524 was exposed to the surface, and the enzymatic acetylation-sensitive K549 appeared to be located near inside of the cave (Fig. 10A). It was observed that K524 was located on a stable α-helix, while K549 was on free active site loop (Fig. 10B). We further examined the amino acids adjacent to the K524 site in three-dimensional microenvironment of the folded structure, and found that positively charged arginine residues (R495, R520, and R534) were located in the vicinity of K524 (Fig. 10C,D). The charge of these arginine residues might attract negatively charged Ac-CoA and AcP. Ac-CoA and AcP molecules were coordinated to the arginine residues, and significantly acetylated K524 site but a little others.
To further evaluate the structural and microenvironmental features that affect lysine reactivity on acetylation, we determined the predicted pKa and buried ration (surface accessibility) of all lysine residues in BsAcsA using Propka3.0 revision 182 (http://propka.org/)21. As shown in Table S1, K524 exhibited pKa value of 9.86 with buried ration of 5%, which was the smallest pKa value among the lysine residues in BsAcsA with buried ration of <10%. Taken together, structural analysis showed that surface accessibility, three-dimensional microenvironment, and pKa value of K524 residue enhance the its reactivity toward AcP and Ac-CoA, and favour nonenzymatic acylation reaction.
In summary, we systematically investigated the site-specific and kinetic characterization of AcuA-catalyzed enzymatic and AcCoA/AcP-dependent nonenzymatic acetylation. B. subtilis acetyl-CoA synthetase (BsAcsA) can be acetylated in vitro either catalytically by lysine acetyltransferase BsAcuA and Ac-CoA (at low concentration), or nonenzymatically by Ac-CoA or AcP (at high concentration). Acetyl-CoA synthetase is inactivated by AcuA-catalyzed acetylation, but not by nonenzymatic acetylation. The results showed that lysine residues on the same protein exhibit different acetylation reactivity with acetyl-phosphate and acetyl-CoA, which was determined by surface accessibility, three-dimensional microenvironment, and pKa value of lysine. The enzymatic and nonenzymatic acetylation revealed different preference for lysine sites (enzymatic acetylation for K549 and nonenzymatic acetylation for K524).
Materials and Methods
Bacterial strains, growth conditions, and chemicals
The strains and plasmids used in this study were listed in Table 2. Bacillus subtilis 168 and E. coli were grown on Luria-Bertani (LB) medium. When needed, antibiotics were added to the medium at the following concentrations: ampicillin, 100 μg/ml; kanamycin, 50 μg/ml. All media were sterilized by autoclaving at 121 °C for 20 min.
The antibodies and columns for purification of proteins used were as follows: anti-acetyl-lysine antibody (ImmuneChem Pharmaceuticals, Burnaby, CA); pan anti-propionyl-lysine antibody (PTM Biolabs); HRP conjugates (anti-PrK; ImmuneChem); glutathione transferase (GST) agarose column and nickel-nitrilotriacetic acid (Ni-NTA) superflow column (Qiangen, Valencia, CA).
Overproduction and purification of proteins
The genes for heterologous expression of protein acetyltransferase BsAcuA and acetyl-CoA synthetase BsAcsA were amplified by PCR from template DNA of B. subtilis 168, and proteins were expressed using the E. coli BL21 (DE3) strain. The primers used in this study are listed in Table S2. The gene coding for AcsA was inserted into the the EcoRI and HindIII sites of plasmid pET-28, and acuA gene was cloned into pGEX-4T-2 that was digested by EcoRI and NotI by one step cloning kit (Novoprotein Scientific, Summit, NJ) respectively, in order to generate a 6-His tag or GST-tag fusion protein. These constructed E. coli BL21(DE3) strains were grown overnight in 5 ml LB medium, and then cultures were transferred into 100 ml LB medium at 37 °C supplemented with kanamycin or ampicillin in shaking flasks. Expression of the cloned genes was induced by the addition of IPTG (0.4 mM), followed by overnight incubation at 20 °C.
Cells were harvested by centrifugation and washed twice wtih 20 mM ice-cold PBS buffer (pH 8.0), resuspended with PBS, then broken by sonication on ice using Ultrasonic cell crusher. Shortly thereafter, cell debris was removed by centrifugation 4 °C. His-tagged proteins were purified using Ni-NTA Superflow columns (Qiangen, Valencia, CA). And bound proteins were eluted using a linear gradient from 20 to 250 mM imidazole 50 mM NaH2PO4 and 300 mM NaCl (pH 8.0). In addition, GST-AcuA was purified by GST affinity chromatography as previously described9. Next, these purified proteins were analyzed by SDS-PAGE. Protein concentration was monitored by the BCA method using buffer PBS as the control and the standard curve was determined by BSA.
In vitro Acyl-CoA synthetase assays
The activity of acetyl-CoA synthetase were determined as previously reported22,23. Briefly, the reaction mixture (100 μl) included 2 mM of acyl substrates (sodium acetate or sodium propionate), 5 mM ATP, 3 mM MgCl2, 0.5 mM dithiothreitol, CoA (2.5 mM), 300 mM hydroxylamine and 50 mM Tris-HCl buffer (pH 8.0). The assay was initiated by adding suitable amount of enzyme, and the reaction mixture was incubated at 37°C for 20 min. The reaction was terminated by adding 100 μl of 2.5% (w/v) FeCl3 in 2 M HCl containing 10% perchloric acid. The denatured protein was removed by centrifugation at 9000 rpm for 5 min, and the absorbance at 520 nm was measured. The standard curves for acyl-CoA were prepared for estimating the enzyme activity, and every sample was averaged from three independent determinations.
Western blot analysis
Protein samples were separated by SDS-PAGE and transferred to a PVDF membrane (Merck, Millipore). The PVDF membrane was blocked at room temperature for 2 h in BSA Blocking Buffer Western Blot I (BSA) (CWBIO, China). After washing with TBST buffer (20 mM Tris-HCl, pH 7.6, 150 mM NaCl, and 0.1%(v/v) Tween 20) three times, the membrane (Millipore) was incubated with anti-acetyl-lysine antibody (Anti-acK) or anti-propionyl-lysine (Anti-prK) antibody which was diluted 1:1000 in TBST at 4 °C for overnight. Then after washing with TBST, the Anti-prK membrane incubated with HRP-conjugated goat anti-rabbit secondary antibody at 1:10000 dilution in TBST for 1 h at room temperature. Eventually, signal detection was tested by ECL system (CTB, USA) according to the manufacturer.
Site-directed mutagenesis of BsAcsA acetylated site
Site-directed mutations at K549 or K524 of BsAcsA were inserted into the pET28a plasmid using the fast mutagenesis system (Transgen Biotech, China) as described previously23. All site-directed genes were amplified by PCR from the template DNA of B. subtilis 168 with the primers listed in Table S2. Next, the DMT enzyme (Transgen Biotech, China) can be directly added to the PCR products systems, and the reaction mixtures were inserted into the EcoRI and HindIII sites of plasmid pET-28 for expression as His tagged fusion proteins by one step cloning kit (Novoprotein Scientific). In order to express protein, these plasmids were transformed into the E. coli BL21 (DE3) strain.
Enzyme-dependent protein acetylation and propionylation
AcuA-catalyzed acetylation and propionylation were carried out as reported22,24. Briefly, purified BsAcsA (10 μg) was incubated at 37 °C with GST-H6-AcuA (10 μg) and 20 μM Pr-CoA (Sigma) or Ac-CoA (20 μM) in HEPES-NaOH buffer (50 mM, pH 7.5) containing tris(2-carboxyethyl) phosphine hydrochloride (200 μM); final volume was 200 µl. After 2 h, samples were divided into two portions for SDS-PAGE and Western blot, meanwhile the other was used for measurement of activity.
In vitro nonenzymatic acetylation and propionylation
Purified BsAcsA (10 μg) were incubated at 37 °C with AcP, Ac-CoA or Pr-CoA (Sigma) in Tris-HCl (50 mM, pH 8.0) containing NaCl (150 mM) for 6 h6,13. After the acylation reaction, samples were analyzed by SDS-PAGE, Western blot, and measurement of the activity.
Identification of in vivo acetylation and propionylation
Bacillus subtilis AcsAWT was introduced into S. enterica Δacs strain18. The resulting strain was grown at 37 °C in the minimal medium (acetate or propionate as sole carbon source). The induced endogenous BsAcsAWT enzymes in S. enterica Δacs strains were analyzed by mass spectrometry for identification of acetylated sites (in acetate minimal medium) and propionylated sites (in propionate minimal medium).
In Gel Digestion and Label-Free Quantification
For sample preparation, gels were excised from the SDS-PAGE gels, then destained with 50% ethanol. After that, the gels were sliced into small pieces and dehydrated with 100% acetonitrile. The proteins in gels were reduced by 10 mM DTT at 56°C for 40 min and alkylated by 15 mM iodoacetamide in darkness for 45 min at room temperature. 50% acetonitrile / 50% 50 mM ammonium bicarbonate (v/v) were used to remove the reagents used and proteins underwent digestion by using trypsin (an enzyme-to-substrate ratio of 1:30). Digested peptides were extracted from the gels by with 50% ACN/50% 0.1% trifluoroacetic acid (v/v) for 30 min and then 100% ACN for 10 min. Peptides were dried in a SpeedVac (Thermo Fisher Scientific, Waltham, MA) before LC-MS/MS analysis17,25.
Peptide samples dissolved in solvent A (0.1% formic acid and 2% acetonitrile in water) were injected onto a reverse-phase C18 column (18 cm length × 75 μm inner diameter; C18 resin with 3 μm particle size; 90 Å pore diameter; Dikma Technologies Inc., Lake Forest, CA) coupled to a nano-HPLC system (Thermo Fisher Scientific, Waltham, MA) and eluted by 60 min gradient with 8−30% solvent B (0.1% formic acid and 10% water in acetonitrile) for 51 min, 30–48% solvent B for 5 min, 48–80% solvent B for 1 min, and 80% solvent B for 3 min at a flow rate of 300 nL/min. LTQ orbitrap Elite mass spectrometer (Thermo Fisher Scientific) was used for analysis with the full MS spectra with an m/z range of 350 to 1700. A mass resolution of 120000 at m/z 200 was set and 15 most intense ions were sequentially isolated for MS/MS fragmentation by using collision-induced dissociation with a normalized collision energy of 35%. Ions with either a single charge or more than four charges were discard. The electrospray voltage was set at 2 kV and dynamic exclusion duration was maintained as 40 s.
Raw data was converted to mgf files by using Thermo Proteome Discoverer v22.214.171.1248, then the mgf files were searched against the Bacillus subtilis database with a Mascot search engine. The search parameters were: enzyme, trypsin/P; missed cleavage, 2; fixed modification, carbamidomethy (C); variable modification, acetyl (protein N-term), oxidation (M) and propionyl or acetyl (K); 10 ppm for MS and 0.5 Da for MS/MS. Qualitative and quantitative analysis were conducted for propionyl or acetyl sites. Areas under the curves (AUC) of the precursor ion’s peak extracted from the ion chromatograms was used to evaluate the MS intensity26. Data were presented by using the normalized intensity. We normalized the protein level by using the peak area of the unmodified peptide ‘HVLSVGEPLNPEVIR’ in each sample. All samples were performed on two replicates.
pKa Calculations and surface accessibility
pKa values and buried ratio of BsAcuA were calculated using propka3.0 (revision 182) (http://propka.org/). And propka calculated the surface accessibility, defining a parameter referred to as buried ratio5,21.
Publisher's note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
This work was supported by grants from the National Natural Science Foundation of China (21335003 and 21575089).