Quantifying morphology of 50 × 20 μm FN nanofibers pre-release and post-release using AFM. (a) Pre-release and (b) post-release FN nanofibers were measured at five transverse locations to determine the width and longitudinally along the center to determine contour length. (c) Nanofiber width was monodisperse and decreased significantly upon release. (d) Similarly, nanofiber length was monodisperse and decreased significantly during release. (e) The pre-lease aspect ratio of the nanofiber increased significantly post-release due to greater contraction along the width than the length. (f) In contrast to the length and width, while nanofiber thickness was monodisperse, it increased significantly during release. (g) Nanofiber volume remained constant between pre-release and post-release nanofibers, indicating the FN was incompressible over this strain range. Scale bars are (a) 10 μm and (b) 2 μm and the symbol ‘#’ indicates a statistically significant difference of P < 0.05.