Quantitative assessment of the blood-brain barrier opening caused by Streptococcus agalactiae hyaluronidase in a BALB/c mouse model

Streptococcus agalactiae is a pathogen causing meningitis in animals and humans. However, little is known about the entry of S. agalactiae into brain tissue. In this study, we developed a BALB/c mouse model based on the intravenous injection of β-galactosidase-positive Escherichia coli M5 as an indicator of blood-brain barrier (BBB) opening. Under physiological conditions, the BBB is impermeable to E. coli M5. In pathological conditions caused by S. agalactiae, E. coli M5 is capable of penetrating the brain through a disrupted BBB. The level of BBB opening can be assessed by quantitative measurement of E. coli M5 loads per gram of brain tissue. Further, we used the model to evaluate the role of S. agalactiae hyaluronidase in BBB opening. The inactivation of hylB gene encoding a hyaluronidase, HylB, resulted in significantly decreased E. coli M5 colonization, and the intravenous injection of purified HylB protein induced BBB opening in a dose-dependent manner. This finding verified the direct role of HylB in BBB invasion and traversal, and further demonstrated the practicability of the in vivo mouse model established in this study. This model will help to understand the S. agalactiae–host interactions that are involved in this bacterial traversal of the BBB and to develop efficacious strategies to prevent central nervous system infections.

neutrophil chemokines IL-8, CXCL-1, CXCL-2, CCL-20 and IL-6 in brain endothelium, and therefore increased the permeability of the BBB 19 . Several investigations in animals have also shown that GBS can penetrate the CNS by crossing the BBB after a prolonged period of bacteremia 20,21 . Therefore, the BBB plays an important role in controlling the entry of pathogens into the brain.
Increased permeability of the BBB can be seen in bacterial meningitis caused by S. agalactiae 22,23 . However, how this bacterium crosses the BBB and enters the CNS is not clearly understood. Therefore, it is of crucial importance to characterize BBB permeability in order to better understand the pathogenesis of meningitis. In our previous study, the inactivation of hylB gene encoding a hyaluronidase, HylB, caused the significantly decreased brain bacterial counts in mice 24 . However, whether HylB directly acts on the BBB opening remains unclear. In this study, we sought to establish a model to evaluate BBB opening by screening a bacterial strain as an indicator, and used this model to quantitatively evaluate the direct role of HylB in S. agalactiae penetration across BBB.

Results
The screening of an indicator strain. To make the counting easy, we aimed to find a bacterium with a colony morphology distinct from that of S. agalactiae. The results showed that among the M1 to M5 E. coli mutants, only the M5 strain generated characteristic blue clones on M63 media (Fig. 1), indicating that it was βgalactosidase-positive.

Determination of E. coli M5 virulence in mice.
To determine whether E. coli M5 was virulent to mice, we performed bacterial infection in BALB/c mice. Interestingly, it was observed that none of the mice infected with 2 × 10 9 CFU of E. coli M5 showed any signs of illness, and there was zero mortality throughout the experimental period of 7 d. The diet and mental state of the experimental mice were identical to those of the control group. The result indicated that E. coli M5 was avirulent to mice.

Kinetics of E. coli presence in blood of mice.
To investigate the rate of E. coli M5 metabolism in mice, the mice were injected intravenously with M5, and blood samples were collected from each mouse at intervals of 3 min, 5 min, 10 min, 30 min, 60 min and 120 min post-infection. The CFU enumeration results showed that the number of M5 cells in the blood increased from 6 × 10 5 CFU/mL at 3 min to a peak value of approximately 2.5 × 10 6 CFU/mL at 5 min and then decreased dramatically to 3 × 10 5 CFU/mL at 10 min. After one hour, bacteria could hardly be detected, and they had clearly been removed from circulation within 2 hours after intravenous injection (Fig. 2). No bacteria were detected in the brain at any time points.
Determination of a challenge concentration of S. agalactiae. We first sought to determine whether the S. agalactiae GD201008-001 strain causes significant BBB permeability by intraperitoneal infection. It is important to determine an appropriate initial inoculation concentration for S. agalactiae to create an in vivo model. We chose values of 5-fold (50 CFU) and 10-fold (100 CFU) that of the median lethal dose (LD 50 < 10 CFU) 25 of S. agalactiae to inoculate the mice. The result showed that with the duration of infection, the number of GD201008-001 cells increased in both the blood (Fig. 3A) and the brain (Fig. 3B), suggesting that GD201008-001 was capable of replicating within the bloodstream and spreading to the brain. GD201008-001 began to appear in the blood at 3 h post-infection, 3 h earlier than in the brain. The bacteria could be detected in the brain at 6 h post-infection when we injected 100 CFU of S. agalactiae. However, we could not detect any  . Detection of S. agalactiae in blood and the brain tissues. (A) Bacterial loads in the blood; (B) bacterial loads in the brain. Groups of four BALB/c mice were inoculated with 50 CFU or 100 CFU of S. agalactiae and killed at different time points post-infection. The CFU of S. agalactiae in the blood and the brain were quantified and described as the mean and S.D. *P < 0.05, **P < 0.01 or ***P < 0.001 indicates significantly different bacterial loads between the two infection groups.
bacteria until 12 h post-infection in the 50 CFU group. This result suggests that the intraperitoneal infection with 100 CFU of S. agalactiae is more appropriate to induce BBB opening in this mouse model. Evaluation of BBB opening. Groups of five mice were challenged with 100 CFU of GD201008-001. At 3 h, 6 h, 9 h and 12 h after challenge, the indicator strain E. coli M5 was inoculated intravenously into each group. In determining the sampling time points, based on Fig. 2, more M5 tracers were detected in the blood at 5 min. Therefore, 5 min after injection was selected to quantify the degree of BBB permeability. Our data showed that GD201008-001 began to appear in the brain 6 h post-infection, and with the duration of infection, the bacterial number increased (Fig. 4). M5 could be detected 3 h after S. agalactiae inoculation, and the number of M5 had a similar increasing trend as GD201008-001 (Fig. 5). This result suggests that E. coli M5 may be used as an appropriate indicator for describing the degree of BBB opening.
Detection of BBB opening caused by S. agalactiae hyaluronidase. Previous study showed that hyaluronidase contributed to S. agalactiae penetration into the CNS 24 . To verify the utility of this mouse model, the mice were infected with the wild-type S. agalactiae, ΔhylB and CΔhylB strains, and then E. coli M5 was administered to the mice. After intraperitoneal infection with 100 CFU of S. agalactiae and its derivatives, the numbers of the three bacterial strains in the blood increased rapidly from <10 3 CFU/mL at 3 h to >10 7 CFU/mL at 15 h (Fig. 6A). In the brains, the wild-type and the complemented strain CΔhylB were present 6 h post-infection, which was earlier than the mutant ΔhylB (Fig. 6B). Compared with the wild-type and complemented strains, for the hylB mutant, the numbers were lower at each time point in the brains. As time passed, the number of bacteria in the brains increased to >10 5 CFU/g at 15 h post-infection. E. coli M5 in the wild-type and CΔhylB groups could be detected in the brain tissues 6 h post-infection, and the increasing trend of the number of M5 cells was similar to that of S. agalactiae (Fig. 6C). The sudden increase in the number of M5 at 15 h indicated that the BBB of the mice had opened to a great degree. However, in the ΔhylB group, the ΔhylB strain and M5 were not detected until 9 h post-infection, and the amount of M5 was significantly lower than that of the wild-type group at 9 h post-infection (P < 0.001). At 12 h and 15 h, the difference in the number of M5 cells between the wild-type and ΔhylB groups reached a highly significant difference (P < 0.001). Although the number of M5 cells in the CΔhylB group was lower than that in the wild-type group, the difference was smaller than that with the ΔhylB group.
To further investigate the role of the hylB gene in BBB opening, the protein HylB, which is encoded by the hylB gene, was expressed and injected intravenously into the mice. An injection of 0.5 mg/mL HylB did not cause BBB  opening at 3 h, as evidenced by the absence of E. coli M5 in brain, whereas M5 could enter and begin to accumulate in the brains of the 1.0 mg/mL and 2.0 mg/mL groups (Fig. 7). There was a dose-dependent increase in the degree of BBB opening at each time point.

Discussion
Meningitis is the most common clinical syndrome of S. agalactiae infection. Bacterial penetration across the BBB and into the CNS is the first step in the development of meningitis 26 . Therefore, adequate BBB models need to be developed in order to characterize the properties of bacterial penetration into the CNS. The in vitro BBB model based on the culture of brain microvascular endothelial cells has been widely used to probe the potential role(s) of individual virulence determinants in the initial pathogenesis of CNS infection by S. agalactiae. For example, hBMEC has been used to determine the invasive roles of fibronectin binding protein A (SfbA) 27 , laminin-binding protein (Lmb) 28 and the surface protein HvgA in GBS infection 29 . However, the in vitro model might not completely mimic the disease in animals or humans. It was reported that CovR-deficient GBS showed a decreased ability to invade the brain endothelium in vitro, but in vivo, this deletion mutant was more proficient in the induction of permeability and proinflammatory signaling pathways in the brain endothelium and in penetration of the BBB 30 . In contrast, a previous study on the major pilin subunit PilB reported that the pilB mutant was less Figure 6. Evaluation of BBB opening in mice infected intraperitoneally with the S. agalactiae wild-type (WT), mutant (ΔhylB), and complemented (CΔhylB) strains. Groups of 40 BALB/c mice were inoculated with 100 CFU S. agalactiae and its derivatives. At 3 h, 6 h, 9 h, 12 h, and 15 h post infection, the blood (A) and brain (B) tissues were collected from four mice of each groups for S. agalactiae quantification. Meanwhile, at each time point, another four mice from each group were injected intravenously with E. coli M5 (2 × 10 8 CFU). Five minutes later, the mice were killed, and the brains were removed for quantification of M5 (C). Bacterial loads in the blood are expressed as CFU/mL, and those in the brains are expressed as CFU/g of tissue. *P < 0.05, **P < 0.01 or ***P < 0.001 indicates significantly different bacterial loads between the two infection groups.
virulent than its wild-type strain in the newborn mice model, whereas, in vitro, the mutant had the similar ability with the wild-type GBS in resistance to macrophage killing 31 . Therefore, the development of an in vivo model will be extremely helpful in the study of bacterial meningitis.
The mouse has been widely used for investigating S. agalactiae virulence 24,27,32 . In recent years, some researchers have developed mouse models by using intravenous injection of exogenous tracers in mice and subsequent detection of extravasate molecules in the brain tissues to measure BBB permeability 33,34 . The tracers provide convenient morphological evidence for BBB opening; however, Saunders et al. 35 reported that the exogenous tracers are not a satisfactory tool for studying blood-brain barrier dysfunction. For example, Evans blue, one of the most commonly used markers, has toxic properties and thus the dye detected in brain might be due to toxic effects on cerebral endothelial or ependymal cells. Also, Evans blue detected in brain is likely to be a mixture of dye bound to plasma proteins, dye bound to brain tissue and free dye. Therefore, it is unreliable to estimate the size of BBB impairment using Evans blue. In this study, we established a model to assess the degree of BBB opening using E. coli M5, which has strong β-galactosidase activity, as an indicator. The bacterial strain is capable of producing blue colonies when cultured on medium containing X-Gal, and cannot permeate an intact BBB. Any entry of this bacterium into and spread within the brain is indicative of a leaky BBB. Therefore, the extent of BBB integrity can be quantitatively assessed through the monitoring of E. coli M5 and the measurement of the bacterial number in brain tissue. The similar method has been reported in S. pneumonia by Tsao et al. 26 Nevertheless, the parameters and criteria may be variable due to different bacterial species. In our study, this model was optimized for use in S. agalactiae, and demonstrated to be a powerful method for analyzing the BBB opening.
Under physiological conditions, the BBB strictly regulates the entry of blood-borne substances into the brain. Brain inflammation can affect the permeability of the BBB directly via cytokine-mediated activation of metalloproteinases or tight junction disruption, or indirectly by promoting transmigration of leukocytes 36 . In S. agalactiae, hyaluronidase has been demonstrated to contribute to the bacterial invasion and the pathogenesis of meningitis in mice 24 . However, unlike the PilA and β-hemolysin/cytolysin which stimulate the release of pro-inflammatory cytokines 18,19 , hyaluronidase acts as an anti-inflammation factor instead. Our previous study demonstrated that compared to the wild-type S. agalactiae, the hyaluronidase-deficient mutant stimulated a significantly higher level of pro-inflammatory cytokines including IL-1β, IL-6 and TNF-α in macrophages, whereas its mortality to zebrafish was lower 24 . Afterwards, a probable reason for this phenomenon was illustrated by Kolar et al. 37 . They revealed that GBS evades host immunity by degrading hyaluronan (HA) which is a component of extracellular matrix nearly in all tissues. HA is commonly cleaved into small fragments by tissue hyaluronidase in response to tissue injury. These small HA fragments are inflammatory factors that ligate to Toll-like receptor (TLRs) to elicit inflammatory response and repair the damaged tissue. However, bacterial hyaluronidase degrades pro-inflammatory HA fragments to the major end product disaccharides. HA disaccharides bind to TLR2/4 to block signaling elicited by host HA fragments and other TLR2/4 ligands, thus preventing GBS ligands from activating pro-inflammatory signaling cascades. Therefore we assume that hyaluronidase contributes to GBS meningitis by anti-inflammation and evasion of host immune. However, our recent study showed that intravenous injection of a purified hyaluronidase, HylB, induced acute lung and brain injury 38 . This led to us to speculate that HylB might play important role in BBB permeability. In order to evaluate this speculation, we first investigated the role of HylB in disrupting the BBB integrity using this model established in this study. Compared with the wild-type S. agalactiae, the inactivation of hylB resulted in decreased BBB opening throughout the infection. Although the presence of S. agalactiae in brain indicates the BBB opening, the use of E. coli M5 as an indicator excludes the possibility that the differential BBB integrity may be caused by different proliferation abilities in vivo between the wild-type and hylB mutant strains.
To further determine whether HylB has a direct impact on BBB integrity, we intravenously injected the purified HylB protein into the mice. We found that the intravenous injection of HylB induced BBB opening in a dose-dependent manner. In the groups treated with 1 mg/mL and 2 mg/mL, the BBB was open 3 h post-infection, 3 h earlier than in the 0.5 mg/mL group, and the number of E. coli M5 increased with the time of infection. In considering the dose of HylB protein, we tested a treatment of 3 mg/mL HylB, but all the mice died 15 h coli M5 (2 × 10 8 CFU) was injected intravenously. Five minutes later, the mice were killed, and the brains were removed for quantification of M5. The data are expressed as CFU per gram of brain. *P < 0.05, **P < 0.01 or ***P < 0.001 indicates significantly different bacterial loads between the two infection groups.
Scientific REPORTS | 7: 13529 | DOI:10.1038/s41598-017-13234-1 post-infection. This finding indicated that HylB is one of the important virulence factors of S. agalactiae, which is in agreement with previous studies 24,38 . A similar role for hyaluronidase in inducing pneumococcal meningitis has also been reported by Zwijnenburg et al. 39 . The present investigation of HylB further demonstrates that using E. coli M5 as an indicator is an easy and reliable method for assessing BBB integrity and/or leakiness. In particular, the model could be more suitable to investigate the contribution of soluble bacterial virulence factors to BBB disruption.
In this study, we used a piscine strain of S. agalactiae with an extremely high virulence to BALB/c mice. It is not clear why this piscine strain is so virulent and what genetic relationship exists between fish and human isolates. Our previous study has made a comparative genomic analysis among 15 S. agalactiae strains of different origins, and found that the Chinese piscine isolates GD201008-001 and ZQ0910 are phylogenetically distinct from the Latin American piscine isolates SA20-06 and STIR-CD-17, but are closely related to the human strain A909 40 . Additionally, a published study reported that a GBS isolate from a clinical case of human neonatal meningitis caused disease and death in Nile tilapia 8 . In this regard, it may be of interest to further investigate the pathogenic mechanisms of meningitis caused by different origins of S. agalactiae strains. This model established here could be a potentially useful tool for the investigation. Nevertheless, it will be imperative to demonstrate that the E. coli tracer works with other GBS and mouse strains that are widely used in the meningitis model.
In summary, the present study developed a model that can quantify the degree of BBB opening caused by S. agalactiae, and used this model to demonstrate that hyaluronidase plays a direct role in BBB permeability.

Methods
Bacterial strains and growth conditions. S. agalactiae strain GD201008-001, β-hemolysin/cytolysin positive, which belongs to serotype Ia, MLST type ST-7, was isolated from farmed tilapia with meningoencephalitis in Guangdong Province, China, in 2010 40 . Its genome sequence has been deposited in the GenBank database under accession number CP003810. The S. agalactiae hylB deleted mutant strain ΔhylB and the complemented strain CΔhylB were constructed in the previous study 24  Determination of E. coli M5 virulence in mice. BALB/c mice (24-26 g, aged 5-6 weeks) were bought from the Experimental Animal Center, Yangzhou University. The mice were divided into two groups with 10 mice for each group. The screened E. coli M5 were grown overnight in LB broth. A bacterial suspension of 50 µL was transferred into 5 mL LB and incubated at 37 °C to allow the cells to reach mid-log phase growth. When the bacteria reached an OD 600 of 0.6, they were harvested by centrifugation at 5000 × g for 5 min. The cell pellets were washed twice with sterile phosphate-buffered saline (PBS) (pH 7.4) and re-suspended in PBS to a concentration of 2 × 10 10 CFU/mL. One group of mice was injected intravenously with 100 μL of bacterial suspension, whereas the other was injected with 100 μL PBS and served as a control. The mice were observed until one week post infection.
Kinetics of E. coli presence in blood of mice. As an indicator, E. coli M5 should be eliminated rapidly from the circulatory system. Based on five predetermined time points, BALB/c mice (24-26 g, aged 5-6 weeks) were divided into five groups with eight mice for each group. Mid-log phase bacteria were washed twice with PBS, followed by re-suspension in PBS and adjustment of the concentration to 2 × 10 9 CFU/mL. For each time point, five mice were used as the experimental group and were injected intravenously with 100 μL of bacterial suspension, while another three were injected intravenously with 100 μL of PBS. Blood samples and brains were obtained aseptically at 3 min, 5 min, 10 min, 30 min and 60 min post infection. Blood samples of 100 μL were spread onto M63 plates. To avoid surface contamination, the organs were washed twice with PBS. Tissues were placed in 1 mL of PBS and homogenized with a biological sample homogenizer (BioPrep-24, Ningbo Hinotek Instrument Co Ltd, China). Then, 100 μL of homogenate that was either undiluted or diluted 10 −1 , 10 −2 and 10 −3 in PBS were plated on M63 plates. The M63 plates were incubated overnight at 37 °C. Colonies were counted and given as CFU/g for brain samples or CFU/mL for blood samples.

Determination of the challenge concentration of S. agalactiae.
Our previous study has shown that the bacterial strain GD201008-001 is highly virulent to BALB/c mice by intraperitoneal administration, with LD 50 values of less than 10 CFU 25 . Here, we chose two different doses of S. agalactiae, 50 and 100 CFU (5-and 10-fold greater than the LD 50 ), to find an applicable dose for this mouse model. BALB/c mice were divided into two groups with 16 mice for each group. One group received an intraperitoneal injection of 100 μL of 500 CFU/ mL bacterial suspension, and the other received an injection of 1000 CFU/mL. In each group, four mice were sacrificed every three hours to aseptically collect the blood and brain. Homogenized brain tissues and blood were plated onto THB plates for bacterial cell counting to determine tissue colonization. The experiments were repeated at least three times to ensure reproducibility. The data are expressed as CFU/g or CFU/mL per mouse.
The mice were infected with a predetermined dose of 100 CFU of the strain GD201008-001. Control mice were injected with sterile PBS. Then, 2 × 10 8 CFU of the indicator E. coli M5 in 100 μL PBS was given intravenously at the specified time points (3 h, 6 h, 9 h and 12 h), and 5 min later, the mice were sacrificed. To detect the degree of BBB opening, the brains were removed aseptically and homogenized in PBS. The homogenate was serially diluted and spread onto THB or M63 agar plates, then incubated overnight at 37 °C. The organ CFU enumeration of S. agalactiae and E. coli M5 were determined and expressed as the mean and S.D. per mouse.
Detection of BBB opening caused by S. agalactiae hyaluronidase. To investigate the effect of BBB opening caused by S. agalactiae hyaluronidase, we used the deficient mutant strain ΔhylB and the complemented strain CΔhylB constructed in our previous study 24 . One hundred and twenty mice were divided into three groups with 40 mice for each group. Mid-log phase S. agalactiae and its derivatives were washed twice in PBS and re-suspended in PBS to 1 × 10 3 CFU/mL. The concentration of E. coli M5 was adjusted to 2 × 10 9 CFU/ mL. Three groups of mice were infected with 100 µL of the wild-type S. agalactiae, ΔhylB or CΔhylB by intraperitoneal injection. At 3 h, 6 h, 9 h, 12 h, and 15 h post infection, groups of four mice were killed, and the blood and brain tissues were collected for S. agalactiae quantification. Meanwhile, another four mice from each group were respectively inoculated with 100 µL of E. coli M5 by intravenous route at each time point as mentioned above. At 5 min post-inoculation with E. coli, the brains were aseptically removed and homogenized in PBS. The homogenates were serially diluted, spread onto THB plates for S. agalactiae counting or M63 plates for E. coli M5 counting and incubated overnight at 37 °C. The bacteria were counted and reported as CFU/g per mouse.
To further determine the role of the hylB gene in the pathogenesis of BBB opening caused by S. agalactiae, we expressed HylB with good enzymatic activity, as described in our previous study 38 . Sixty mice were divided into three groups with 20 mice in each group. The three groups of the mice were intravenously injected with 200 μL of HylB protein at a final concentration of 0.5 mg/mL, 1.0 mg/mL and 2.0 mg/mL, respectively. Another 20 control mice were injected with 200 μL of sterile PBS. Then, 100 μL of the E. coli M5 (2 × 10 9 CFU/mL) was inoculated intravenously at intervals of 3 h, 6 h, 9 h, 12 h and 15 h post infection, and the brain tissues were sampled at 5 min post-infection with E. coli. The homogenates were serially diluted, spread onto M63 plates and incubated overnight at 37 °C for E. coli M5 counting. The bacteria were counted and expressed as CFU/g per mouse.
Statistical analysis. Data were collected and analyzed using MS Excel 2010 and SPSS Statistics version 20.0 software. Multiple comparisons were performed by analysis of variance (ANOVA) followed by Turkey's multiple-comparison test, with P < 0.05 indicating a statistically significant difference and P < 0.01 indicating a highly significant difference. The error bars presented in the figures represent the standard deviations of the means of multiple replicate experiments.

Experimental procedures
Ethics statement. All the animal experiments were carried out according to animal welfare standards and were approved by the Ethical Committee for Animal Experiments of Nanjing Agricultural University, China. All animal experiments complied with the guidelines of the Animal Welfare Council of China.