Figure 7: | Scientific Reports

Figure 7

From: N-terminal gelsolin fragment potentiates TRAIL mediated death in resistant hepatoma cells

Figure 7

Inhibition of p53 expression decreased the loss in cell viability in p53 positive cells treated with an N-terminal gelsolin1–70 fragment and TRAIL. Treatment of HepG2 cells with siRNA to p53 prior to exposure to the N-terminal gelsolin1–70 fragment and TRAIL enhanced cell viability after 48 hours of treatment (panel A). Treatment of HepG2 cells with siRNA to p53 prior to exposure to the N-terminal gelsolin1–70 fragment and TRAIL inhibited phosphorylation of p53 at S15 and enhanced p21 expression by Western blot analysis (panel B). Densitometric scanning results are shown on the right. Each panel is representative of three independent experiments and mean values ± SD are shown as error bars. Note that cropped gel images are used and the gels were run under the same experimental conditions.