Figure 3: | Scientific Reports

Figure 3

From: N-terminal gelsolin fragment potentiates TRAIL mediated death in resistant hepatoma cells

Figure 3

CM and TRAIL reduces association between p53 and Mdm2. p53 and Mdm2 phosphorylation was analyzed in CM, TRAIL, and CM + TRAIL treated HepG2 cells (panels A and B). Cellular phosphorylation status of p53 at Serine15 residue was apparent when cells were exposed to both CM and TRAIL, but total p53 levels were not affected. Phosphorylated Mdm2 was reduced in the presence of both CM and TRAIL (panel B). Densitometric scan data is provided. Note that cropped gel images are used and the gels were run under the same experimental conditions. Subcellular localization of p53 and Mdm2 is shown by immunofluorescence (panel C). Co-localization of p53 and Mdm2 was noted in HepG2 control cells, and their association noted in the presence of TRAIL. This interaction was not observed in cells treated with CM and TRAIL when examining multiple fields. Each panel is representative of three independent experiments.