Treatment of TRAIL insensitive transformed hepatic cells with CM and/or TRAIL enhances death sensitivity. HepG2 and Huh7 cells (panel A) were treated for 24 hours with CM as a source of gelsolin fragment, TRAIL (50 μg/ml and 100 μg/ml, respectively) or both CM and TRAIL. TRAIL-R1 receptor expression was analyzed in HepG2 (panel B) and Huh7 (panel C) cells by Western blot. Note that cropped gel images are used and the gels were run under the same experimental conditions. Densitometric scanning of the Western blot results are shown at the bottom of respective panels. Values represent mean from three independent experiments ± SD and are shown as error bars. Statistical significance was analyzed using the two-tailed Student’s t-test: *P < 0.05, **P < 0.01.