Flow Cytometric Analysis for Tumor Infiltrating Lymphocytes 5 Days After aFP Treatment in the One Tumor Mouse Model. Flow cytometry analysis was performed to confirm whether CD3+ and CD8+ lymphocyte, and regulatory T cell (Treg) numbers were affected by aFP. (a) Proportion of CD3+, CD8+ and CD4+ T cells and Treg normalized to tumor weight. (b) Ratio of CD8+ T cells to Tregs (CD4+ Foxp3+). (c) Proportion of antigen specific CD8+ T cells normalized to tumor weight and the percentage of antigen specific CD8+ T cells normalized to total number of CD8+ T cells. The bars represent SD. * P < 0.05, **P < 0.005.