Tumor Volume and Survival Curves after Treatment and Rechallenge Test in the One Tumor Mouse Model. Mice were inoculated unilaterally at the left leg with 3.5 × 105 CT26WT subcutaneously into the depilated thigh. aFP laser irradiation was performed 8 days after tumor inoculation, and then the growth of the tumors was observed. Anti-PD-1 blocking antibodies were administered intraperitoneally at a dose of 200 µg per mouse on days 8, 10, 12, 14, and 16 in the one tumor mouse model after tumor cell inoculation. (a) Tumor volume curves of mice in the control, anti-PD-1, aFP and aFP + anti-PD-1 groups after tumor inoculation. (b) Kaplan-Meier survival curves of mice receiving tumor inoculation. The significance values for the difference between the survival curves are: Control vs. anti-PD1: P < 0.05, Control vs. aFP: P < 0.0001, control vs. aFP + anti-PD1: P < 0.0001, anti-PD-1 vs. aFP: P < 0.05, anti-PD1 vs. aFP + anti-PD1: P < 0.0002. (c) Kaplan-Meier survival curves of mice receiving the rechallenge test with CT26WT cells. These mice were each inoculated with 3.5 × 105 CT26WT cells subcutaneously in the contralateral (right) thigh against previous inoculation. The significance values for the difference between the survival curves are: control mice vs. survival mice (p < 0.005), control vs. aFP + anti-PD1: P < 0.0001. *The bars represent SD. P < 0.05, **P < 0.005, ***P < 0.0005, ****P < 0.0001.