Insecticidal activity and the mechanism of action of three phenylpropanoids isolated from the roots of Piper sarmentosum Roxb

Hexane, dichloromethane and methanol extracts of the roots of Piper sarmentosum Roxb. were screened for toxicity towards Sitophilus oryzae (L.), Rhyzopertha dominica (F.), and Plodia interpunctella (Hübner) and the hexane extract exhibited the highest mortality percentage. Bioassay-guided fractionation of the hexane extract resulted in the isolation of asaricin 1, isoasarone 2, and trans-asarone 3. Asaricin 1 and isoasarone 2 were the most toxic compounds to Sitophilus oryzae, Rhyzopertha dominica, and Plodia interpunctella. Sitophilus oryzae and Rhyzopertha dominica exposed to asaricin 1 and isoasarone 2 required the lowest median lethal time. Insecticidal activity of trans-asarone 3 showed consistent toxicity throughout the 60 days towards all three insects as compared to asaricin 1 and isoasarone 2. Asaricin 1 and isoasarone 2 at different doses significantly reduced oviposition and adult emergence of the three insects in treated rice. Trans-asarone 3 had lowest toxicity with highest LC and LT values in all tested insects relative to its mild oviposition inhibition and progeny activity. Moreover, asaricin 1 and isoasarone 2 significantly inhibited acetylcholinesterase in comparison with trans-asarone 3 and the control. Acetylcholinesterase inhibition of Rhyzopertha dominica and Plodia interpunctella by asaricin 1 and isoasarone 2 were lower than that of Sitophilus oryzae, which correlated with their higher resistance.

Storage pests have a direct effect on the reduction of the quantity and quality of grain during postharvest storage. The damages brought upon stored grains and their related grain products by insects could be as high as 20-30% in tropical countries such as Malaysia [1][2][3] . Sitophilus oryzae (L.), Rhyzopertha dominica (F.), and Plodia interpunctella (Hübner) are considered as main pests of stored food 4 . These insects can infest a wide range of stored grains such as wheat and corn. The adults of S. oryzae and R. dominica are known to attack and consume the intact grains while their larvae feed on the kernel and develop inside of it 5,6 . Plodia interpunctella, also known as the Indian moth larva, during its adult stage does not cause much damage as compared to its larval stage 7 . P. interpunctella is an external feeder. The larva continuously produces a silk web around the surface and inside of the food and feeds within the web 8 .
Synthetic insecticides are commonly used to protect stored grains and their related products against pests. The mechanism of toxicity of most insecticides such as organophosphorus and carbamate compounds is based on the inhibition of acetylcholinesterase [9][10][11] . In insects, acetylcholinesterase (AChE) hydrolyzes the neurotransmitter acetylcholine (ACh) to terminate neuronal excitement at the postsynaptic membrane 12 . Repeated usage of synthetic insecticides has resulted in poisoning non-targeted organisms, residual contamination and the increased resistance in insect pests [13][14][15] . These problems have warranted the need for developing alternative strategies which include using ecofriendly products in particular plant derived compounds [16][17][18] .
Plants offer an alternative source of insect-control agents as they contain a wide range of bioactive compounds, many of which are selective and have little or no harmful effects on non-targeted organisms and the environment unlike synthetic insecticides 16,19,20 . Plant extracts have been employed in many traditions as insecticides even before the advent of synthetic insecticides. Their potential in controlling insect growth has been used to store

Results
Insecticidal activity with treated grains. The toxicity potential of the hexane (HE), dichloromethane (DE) and methanol extracts (ME) from the roots of P. sarmentosum against S. oryzae, R. dominica, and P. interpunctella are presented in Table 1. Based on the results obtained, the hexane extract exhibited the highest percentage of mortality for all three storage pests at a concentration level of 500 µg/mL over an exposure period of 72 hours (Table 1). Hence, HE was subjected to toxicity-guided fractionation which gave faction 2 (F2) as the active fraction (Table 1). Potent toxicity of F2 led to the isolation and characterization of asaricin 1, isoasarone 2, and trans-asarone 3 as the active constituents (Fig. 1).
Evaluation of lethal concentration of the compounds. Asaricin 1 and isoasarone 2 showed high toxicity against all three tested insects. The LC 50 of S. oryzae was estimated to be 4.7 µg/mL for asaricin 1 and 5.6 µg/ mL for isoasarone 2 which considered the lowest LC 50 among the tested insects (Table 2). Similarly lowest LC 95 value was observed for S. oryzae in response to asaricin 1 (13.6 µg/mL) followed by isoasarone 2 (14.3 µg/mL). P. interpunctella was more tolerant to the toxicity of asaricin 1 and isoasarone 2 with LC 95 value of 35.9 and 37.7 µg/ mL respectively. Evaluation of lethal time of the compounds. The lethal time (LT 50 and LT 95 ) by each isolated compound studied on S. oryzae, R. dominica, and P. interpunctella was presented in Table 3. Smaller LT 50 and LT 95 values of asaricin 1 and isoasarone 2 indicated both compounds had faster action than trans-asarone 3. However, S. oryzae and R. dominica exposed to asaricin 1 and isoasarone 2 had lower LT 50 values ranging from 15.7 hours to 18.4 hours as compared to P. interpunctella (46.9 hours). Trans-asarone 3 showed the highest LT 50 (≥38.9 hours) and LT 95 values (≥79.2 hours). By comparing of 95% confidence interval there was no significant difference (p > 0.05) between the LT values observed from asaricin 1 and isoasarone 2. P. interpunctella had the significant highest LT values as compared to S. oryzae and R. dominica.

Contact toxicity.
The results clearly demonstrated that asaricin 1, isoasarone 2, and trans-asarone 3 did not show potent contact toxicity against stored grain insect pest even at the highest concentration of 200 μg/mL (Fig. 2). The efficacy in respect to the toxicity of asaricin 1, isoasarone 2, and trans-asarone 3 towards S. oryzae Residual toxicity using LC 95 value of each active compound. The mean mortality of S. oryzae, R.
dominica and P. interpunctella were recorded during the 60 days of bioassay (Fig. 3). During the 60 days of residual activity test, the mean mortality of S. oryzae (F = 3.6, p < 0.05), R. dominica (F = 4.6, p < 0.05) and P. interpunctella (F = 4.4, p < 0.05) were significantly higher than the control after treated with asaricin 1, isoasarone 2 and trans-asarone 3. The efficacy of asaricin 1 and isoasarone 2 with their relative LC 95 against S. oryzae was consistent during the first 30 days and slowly decreased and this pattern continued till last day of the assay. On the other hand, toxicity efficacy of trans-asarone 3 towards S. oryzae with high LC 95 value of 670.2 μg/mL was consistent till the 60 th day. R. dominica and P. interpunctella response to residual effects of asaricin 1, isoasarone 2, and trans-asarone 3 were similar and followed almost the same pattern as S. oryzae. Asaricin 1 and isoasarone 2 showed consistent toxicity against tested insects during the first 30 days according to the mortality rate. Then, the insecticidal activity reduced and by the 60 th day, it was at the lowest point (Fig. 3).
dominica, and P. interpunctella (Table 4). S. oryzae and R. dominica were repelled more by asaricin 1 and isoasarone 2 with mean repellency of 79-85% while P. interpunctella was less repelled with mean repellency of 50-58% after 20 hours. Trans-asarone 3 was less effective in repellency with less than 10% repellency for all tested insects. F1 progeny activity. Table 5 shows the effect of F1 progeny activity of asaricin 1, isoasarone 2, and trans-asarone 3 on S. oryzae, R. dominica, and P. interpunctella. Asaricin 1 and isoasarone 2 were highly potent on inhibition of S. oryzae, R. dominica, and P. interpunctella emergence of the adult insects at low dosage as 2 µg/ mL. The asaricin 1 and isoasarone 2 at 4 µg/mL concentrations were able to inhibit 100% of the emergence of adult S. oryzae and R. dominica. P. interpunctella were more resistance to asaricin 1 and isoasarone 2 where they only caused 78.98 and 86.37% inhibition of F1 adult emergence at 4 µg/mL. Meanwhile trans-asarone 3 was weak in inhibition of F1 adult emergence as compared to asaricin 1 and isoasarone 2. All the tested isolated compounds were significantly (p < 0.05) different from the controls in their effects.  Table 2. LC 50 and LC 95 of asaricin 1, isoasarone 2, and trans-asarone 3 against S. oryzae, R. dominica and P. interpunctella after 72 hours exposure. *Asaricin 1, isoasarone 2 and trans-asarone 3 represent phenylpropanoids isolated from P. sarmentosum hexane active fraction (F2). **Test performed on S. oryzae and R. dominica two weeks old adults and P. interpunctella third instar larvae. ***LC 50 and LC 95 values significant difference (p < 0.05) is based on non-overlap of the 95% confidence interval (C.I.). ****Since Chi square goodness of fit test is not significant (p > 0.15), no heterogeneity factor is used in the calculation of fiducial limits.  Table 3. LT 50 and LT 95 of asaricin 1, isoasarone 2, and trans-asarone 3 against S. oryzae, R. dominica and P. interpunctella after 72 hours exposure. *Test performed on S. oryzae and R. dominica two weeks old adults and P. interpunctella their instar larvae. **LT 50 and LT 95 values significant difference (p < 0.05) is based on non-overlap of the 95% C.I. ***Since Chi square goodness of fit test is not significant (p > 0.15), no heterogeneity factor is used in the calculation of fiducial limits.

Compounds
Scientific REPORTS | 7: 12576 | DOI:10.1038/s41598-017-12898-z Oviposition deterrent effect. The ovipositional inhibition effect is demonstrated in Fig. 4. The egg deposition was recorded 72 hours after exposure of insects to asaricin 1, isoasarone 2, and trans-asarone 3. Cumulative number of egg laying by the S. oryzae, R. dominica, and P. interpunctella females were highly inhibited by asaricin 1 and isoasarone 2. These results indicated that asaricin 1 and isoasarone 2 significantly suppressed the egg deposition tested insects. Asaricin 1 and isoasarone 2 caused 10 to 35% reduction of egg deposition at the lowest dosage of 0.5 µg/mL in all tested insects. At concentration of 4 µg/mL, asaricin 1 and isoasarone 2 caused 100% ovipositional inhibition in S. oryzae and R. dominica and up to 68-77.9% inhibition in P. interpunctella. Overall, the statistical analysis of proportions of egg laying by insect pests between control and asaricin 1 were found to be statistically significant for S. oryzae (F = 7.6, p > 0.05), R. dominica (F = 5.1, p > 0.05) and P. interpunctella (F = 4.4, p > 0.05) which was similar to isoasarone 2. On the other hand, trans-asarone 3 had weak ovipositional inhibition in all tested insects where it could not reduce the egg laying after treatment. There was no significant differences in trans-asarone 3 and control (F = 3.9, p < 0.05).
Cholinesterase Enzymes Inhibitory Activity. The initial AChE inhibition activity of the three compounds was evaluated at their relative LC 50 on the enzyme supernate extract from each insect. Asaricin 1 and isoasarone 2 showed potent AChE inhibition for all three insects. Asaricin 1 and isoasarone 2 which showed high toxicity towards S. oryzae (LC 50 value of 4.7 and 5.6 μg/mL respectively) also showed high AChE inhibition on enzyme supernate extracted from S. oryzae. Both asaricin 1 and isoasarone 2 exhibited a slightly lower AChE inhibition of the extracted enzyme from R. dominica and P. interpunctella as compared to that of S. oryzae. The lower AChE inhibition can relate with the relative higher LC value for R. dominica and P. interpunctella. In general, asaricin 1 exhibited the highest acetylcholinesterase inhibition followed by isoasarone 2. Trans-asarone 3 exhibited a lower inhibitory activity of less than 30% as compared to asaricin 1 and isoasarone 2 for all tested insects. The LC 50 value and percentage of AChE inhibition had significant negative correlation (Fig. 5). The correlation study between the LC 50 and mean percentage of AChE inhibition was significant (p > 0.05). The results of correlation coefficient is presented in Table 6. The correlation study on AChE inhibition of S. oryzae was meaningful and negative for asaricin 1 and isoasarone 2 (correlation coefficient (r) of asaricin 1: −0.920 and isoasarone 2: −0.946) ( Table 6). R. dominica and P. interpunctella correlation study was almost similar to S. oryzae.

Discussion
Bioassay-guided fractionation of the active hexane extract resulted in the isolation and characterization of asaricin 1, isoasarone 2, and trans-asarone 3. Asaricin 1 and isoasarone 2 exhibited potent insecticidal activity against S. oryzae, R. dominica and P. interpunctella. The lowest LC 50 and LC 95 values for S. oryzae were observed for asaricin 1 and isoasarone 2 among the tested insects while P. interpunctella was more resistant to the toxicity of asaricin 1 and isoasarone 2. Based on the LC 50 values and their respective 95% confidence intervals, trans-asarone 3 was found to be significantly less effective towards S. oryzae and R. dominica adults and P. interpunctella larvae. The LC 50 and LC 95 values for all three species were variable and species specific. This result is supported by other reports, which showed great variations in the susceptibility of insects to other biopesticide product 34 . interpunctella larvae on rice grain treated with asaricin 1, isoasarone 2, and trans-asarone 3 by using their relative LC 95 value, exposed from 0 to 60 days after treatment. Percentage of mortality for trans-asarone 3 were significant stable as compared to asaricin 1 and isoasarone 2 after 30 th days.
The percentage of variation in the sensitivity of stored grain insect pests is high in response to toxicity of volatile compounds. Similar results have been reported by several researches on insecticidal activities of other plant extracts 35,36 . Besides, the results of lethal time (LT 50 and LT 95 ) showed fast acting of the asaricin 1 and isoasarone 2 against the S. oryzae, R. dominica and P. interpunctella 37,38 . The fast and significant mortality indicated the potent insecticidal activity of asaricin 1 and isoasarone 2. Furthermore, none of these compounds exhibited potent contact toxicity while asaricin 1 and isoasarone 2 were highly toxic against the pests in treated grain assay. According to previous research some natural compounds or oils express toxicity against stored product pests, such as l-carvone, d-carvone, horseradish oil, and mustard oil, but not all are active against insect pests through contact toxicity 39,40 . The results suggested that all three compounds act as stomach poison as the toxicity may be due to ingestion and digestion of the compounds in the stomach but not by absorption through insects' curticle 41,42 . To confirm this statement, further investigations are needed after the formulation of the compounds. On the other hand, extended period of protection, repellency activities, F1 progeny activity and anti-ovipositional properties of asaricin 1 and isoasarone 2 suggested their excellent grain protectant potential.
Asaricin 1 and isoasarone 2 could provide protection against S. oryzae, R. dominica and P. interpunctella over a relatively long period (over 30 days) according to residual toxicity test. However, insecticidal activity of trans-asarone 3 showed consistent toxicity throughout the 60 days towards the three stored grain pests. This observation suggested that trans-asarone 3 has a longer half-life as compared to asaricin 1 and isoasarone 2. This result may be due to stability of trans-asarone 3. The three compounds have carbon double bonds in the aromatic ring and alkyl side chain. However, trans-asarone 3 has conjugated double bonds while asaricin 1 and isoasarone 2 have isolated double bonds. Conjugated double bond has resonance stabilization, so it is more stable than isolated double bond. Hence, trans-asarone 3 may be have better stability and resistance to oxidative degradation which resulted in longer half-life in comparison to asaricin 1 and isoasarone 2 43,44 . Although the insecticidal activity of trans-asarone 3 was the lowest (LC 50 value of 258.90-432.42 μg/mL) but since its toxicity effect was consistent during the 60 days, it may be a good option to be used in mixtures with asaricin 1 and isoasarone 2 as it is most probably more stable.

Compounds Insects
Repellency (mean ± SE)*   Table 5. Effect of asaricin 1, isoasarone 2, and trans-asarone 3 against on the adult emergence of three stored product insect pests infesting rice. *Test performed on S. oryzae and R. dominica two weeks old adults and P. interpunctella their instar larvae. **% IR (inhibition rate) values followed by a common letter are not significantly different (p < 0.05).
As shown in Fig. 6, the presence of asaricin 1, isoasarone 2 and trans-asarone 3 as acetylcholinesterase inhibitors can cause inhibition of AChE from breaking down to neurotransmitter; acetylcholine, which disrupt or increase the duration of action of the neurotransmitter 9,11 . Mechanism of action asaricin 1 and isoasarone 2 maybe has similarity with organophosphate insecticides due to their strong AChE inhibition. The inhibition of AChE may has direct effect on bradycardia, bronchoconstriction and prolonged muscle contraction of insects which lead to paralysation and death 45 . The biochemical assay revealed the possible connection between the AChE inhibition and the toxicity of the three compounds. These results suggested that there was significant and negative relationship between AChE inhibition and LC value where higher inhibition of AChE causes stronger toxicity and results in lower LC 50 value. The difference in the AChE inhibition for all three compounds may be Scientific REPORTS | 7: 12576 | DOI:10.1038/s41598-017-12898-z due to the insect resistance and different binding modes due to differences in AChE structural features in each species studied 46,47 . This is the first study conducted on the insecticidal activity and the mechanism of action of the compounds isolated from P. sarmentosum in insect pest control. The results obtained from this study suggested the potential use of P. sarmentosum extracts and its active components in storage pest management. In addition, further investigations regarding the safety issues for mammalian and environmental health, antifeedant activity and formulations will be conducted. Formulations may improve and enhance the insecticidal potency of the compounds.

Materials and Methods
General experimental procedures. General experimental procedures were similar to those described by Hematpoor et al. 31 .
Plant material. The plant material was collected as previously described 31  Extraction and Isolation. The roots of P. sarmentosum were extracted as previously described 31 . The highest percentage of mortality was observed for the hexane extract in a preliminary screening of the potential toxicity of the extracts towards S. oryzae, R. dominica, and P. interpunctella (Table 1). Hence, the hexane extract was subjected to bioassay-guided fractionation and fraction F2 from total of eight fractions was found to exhibit the highest toxicity towards S. oryzae, R. dominica, and P. interpunctella (Table 1). Asaricin 1, isoasarone 2, and trans-asarone 3 were isolated from the active fraction F2 as previously described 31 .
Structural elucidation of compounds. The structures of the isolated compounds (asaricin 1, isoasarone 2, and trans-asarone 3) were characterized using spectroscopic data as previously described 31 and compared with literature values 48-50 . Insecticidal activity. Test insects. All tested insects used for the experiments were obtained from colonies cultured in the laboratory at 25 ± 1 °C and 65 ± 5% relative humidity. S. oryzae, R. dominica, and P. interpunctella were reared on rice grains with additional 5% yeast. Two weeks old adults of S. oryzae and R. dominica and 3 rd instar-larvae of P. interpunctella were used for the bioassay.
Insecticidal effects on treated grain. Bioassay was conducted by dissolving 1 mg of each extract (hexane, dichloromethane and methanol) in 1 mL of acetone and introducing them onto 5 g of rice grains using a pipette. The control was only treated with 1 mL of acetone. The treated grains were dried under the fume hood. Twenty x represents mean percentage of AChE inhibition, y represents relative LC 95 of asaricin 1, isoasarone 2 and trans-asarone 3 against S. oryzae, R. dominica and P. interpunctella.
Scientific REPORTS | 7: 12576 | DOI:10.1038/s41598-017-12898-z unsexed adults of S. oryzae and R. dominica were separately introduced into the respective petri dishes containing 5 g of treated rice. Twenty 3 rd instar larvae of P. interpunctella were carefully collected with a fine brush and placed in each petri dish (10 cm diameter × 1.5 cm). All treatments were monitored to obtain the mean percentage of mortality after 72 hours. Experiments were replicated four times. The same technique was used to determine the bioactivity of each fraction obtained from the active extract against the test insects. Compounds isolated from the active fraction were evaluated for their toxicity towards S. oryzae, R. dominica and P. interpunctella to obtain their lethal concentration values (LC). Five grams of rice grains were treated with different concentrations (w/v) of each compound. Controls were treated with acetone. The treated grains were allowed to dry at room temperature (27 °C). The petri dishes were covered and sealed with parafilm. Mortality was recorded after 24, 48, and 72 hours of exposure.
Contact toxicity. Residual film bioassay technique was used to study the contact toxicity of asaricin 1, isoasarone 2, and trans-asarone 3 1,25 . Amount of 1 mg of each compound was dissolved in 1 mL of acetone and used as the stock solution to apply in several dosages to determine their contact toxicity. After a serial dilution, dosages between 20-200 µg/ mL were carefully introduced onto filter papers (Whatman No. 1). Controls received only acetone. After drying under a fume hood for 15 minutes, each filter paper was placed at the bottom of a petri dish. Twenty adults of S. oryzae and R. dominica and twenty larvae of P. interpunctella were introduced into each petri dish, respectively. The petri dishes were covered and sealed with parafilm. Mortality of tested insects were recorded after 24 and 48 hours of treatment and percentage of mortality of each compound were calculated using Abbott's formula 51 . Abbott's formula is as follows: where: X = percent live in control. Y = percent live in treatment.
Residual toxicity against storage pests. Residual toxicity against adults of S. oryzae and R. dominica, and larvae of P. interpunctella were assisted by optimization of the method from Islam and Talukder, 2005 52 . The assay was carried out in separate plastic cups for a period of 60 days after 15 g of rice were treated with asaricin 1, isoasarone 2, and trans-asarone 3. The length of exposure and the dosage of each compound were chosen as the concentration required to cause 95% mortality of the individuals. The LC 95 values obtained from the earlier study (section 2.5.2) was used to evaluate the persistence of toxicity during the 60 days period. After each treatment was completed, the plastic cups were closed with their caps, secured and held at room temperature (27 °C). The control was only treated with acetone. These bioassays were carried out over a period of 60 days whereby on the 7 th day, 30 insects were placed in each plastic cup. After 72 hours, the insects were removed from each cup. Insect's mortality was recorded and the dead insects were discarded. All cups were tightly closed until the next bioassay. The procedures were repeated on the 15 th , 20 th , 30 th , 40 th , 50 th and 60 th day. All bioassays were replicated 4 times.
Repellency test. Repellency of asaricin 1, isoasarone 2, and trans-asarone 3 was evaluated using area preference methods 25 . Whatman No. 1 filter papers were cut into halves. One side treated with 100 µL/mL of asaricin 1, isoasarone 2 and trans-asarone 3 respectively using pipette. The other half was treated with acetone as control.
The treated and control half discs were air dried under laboratory conditions and full discs re-made using paper tapes to attach together. Each paper was placed in a petri dish and ten S. oryzae was introduced at the centre of the filter paper then the petri dish was closed and sealed with parafilm. Each treatment was replicated four times and the number of S. oryzae present on each side was recorded every 5 hours up to 20 hours. The same procedures were applied for R. dominica and P. interpunctella. The Percentage of Repellency (PR) calculated as follows: Negative PR value were treated as zero.
F1 progeny activity of asaricin 1, isoasarone 2, and trans-asarone 3. Fifty grams of rice grains were weighed and then three concentrations (1, 2, and 4 μg/mL) of each compound asaricin 1, isoasarone 2, and trans-asarone 3 were individually mixed and specimen were deposited in a 300 mL of plastic containers. The compounds were dissolved in acetone and uniformly spread on rice. Rice treated with acetone only was set as control. Ten pairs of adult insects were introduced into each container. Four replicates of each treatment and untreated controls were laid out in Complete Randomized Design. Adult mortality was counted and recorded after 48 and 72 hours of application. On the 4 th day, all insects whether dead or alive, were removed and experiments were left for 35 days to allow for emergence of F1 generation. The number of adults emerged was counted after 35 days and inhibition rate (% IR) in adult emergence was calculated using following formula 53 .
n n n where: C n = number of emerged insects in the control. T n = number of emerged insects in the treated container.
Oviposition deterrent activity. The asaricin 1, isoasarone 2, and trans-asarone 3 were tested for their oviposition inhibition activity 54 . Three concentrations (0.5, 1, 2, 3, and 4 μg/mL) for each compound were prepared by dissolving them in acetone. Twenty undamaged grain of rice were placed in the plastic petri dish and treated separately with different dose of asaricin 1, isoasarone 2, and trans-asarone 3 in uniform manner. The seeds were air dried until all acetone evaporated. For control seeds were dressed in 1 mL acetone only. The treated samples were kept in laboratory condition (27 ± 2 °C and 80 ± 5% RH). Five males and five females were placed on each treatment. After 48 hours the mortality of each insect was observed in each petri dish and all insects (live and dead) were removed. The number of eggs laid on seeds of treated and control seeds were counted after three days of starting the experiment. To evaluate the number of eggs laid for S. oryzae on rice grains samples were recorded after staining the grains to detect the egg plugs 55 . Biochemical assays. The tested insects were respectively subjected to acetylcholinesterase biochemical assay (AChE) 56 . Each insect was removed and homogenized at 0 °C in 0.05 M-phosphate buffer, pH 7.5 (KH 2 PO 4 -NaOH) (Sigma) using a glass homogenizer pestle (5%, w/v, homogenate). Plastic tube covered with ice were used to keep the homogenized mixture. The homogenates were centrifuged at 16,000 rpm for 20 min (5 °C). These supernatants were kept in the ice and used without further purification for studying the acetylcholinesterase and its inhibition. To determine the AChE inhibition activity, procedures from Ellman et al., 1961 were slightly modified and used 57,58 . 10 μL of enzyme supernatants were transferred to each well of a 96-well microtiter plate using electronic multichannel pipette (Eppendorf, USA) then mixed with 20 μL of tested compounds in solution form and 150 μL of phosphate buffer which were kept at 1-4 °C temperature were added. Asaricin 1, isoasarone 2, and trans-asarone 3 were dissolved in DMSO (Merck, USA) as AChE inhibitor at their LC 95 value and 0.1% DMSO (v/v) were added into test well in separated rows. The microtiter plates were incubated for 10 minutes at 25 °C. Next step was followed by adding 20 μL of acetylthiocholine iodide (ACTHI) (Sigma-Aldrich, USA) (0.4 mM) and DTNB (Sigma-Aldrich, USA) (0.3 mM) to enzyme supernatants solution to observe the reaction. During the 30 minutes reaction at room temperature, yellowish or colorless solution was observed. The microtiter plates were sentenced for microplate readers (Synergy H1 Hybrid Multi-Mode Microplate Reader, USA) at 412 nm and results were recorded. Percentage of inhibition calculated based on the mean optical density of enzyme as below: Absorbance sample Absorbance of background Absorbance of blank Absorbance of background 100% (5) The resulting data for each selected insect was analyzed using correlation study compare the enzyme expression levels between different insects in response to asaricin 1, isoasarone 2, and trans-asarone 3. All levels of statistical significance were determined at p < 0.05. Data Analysis. Bioassay data within the range of 5-95% were pooled and subjected to probit analysis 59 by using Polo Plus (LeOra Software, Berkeley, CA) to obtain 50% and 95% lethal concentrations (LC 50 and LC 95 ). Data on adult mortality were also subjected for determining lethal time required by each isolated compound to kill 50% and 95% (LT 50 and LT 95 ). Significant differences in the LC 50 and LC 95 values were based on non-overlap of 95% confidence intervals 60 . Abbott's formula 51 was applied to correct the percentage of mortality if the control mortality was more than 5%. The analyses of the data were done using ANOVA followed by Tukey's test using SAS version 9.1.3, 2002 software. Statistical correlation study was conducted to understand the relation between acetylcholinesterase activity and LC 50 dose of asaricin 1, isoasarone 2, and trans-asarone 3 levels of statistical significant were determined at p < 0.05. Data availability statement. All data generated or analysed during this study are included in this published article.