Abstract
Chicken Ovalbumin Upstream Promoter-Transcription Factor II (COUP-TFII) plays pivotal roles in cell growth, cell differentiation, and cell fate determination. Although genome-wide studies have identified COUP-TFII binding on gene sets mainly involved in neural crest cell (NCC) development and craniofacial morphogenesis, the direct functional connection between COUP-TFII and NCCs in vivo has not been well characterized. In this study, we show that COUP-TFII is expressed in the subpopulation of NCCs and its derivatives, and targeted ablation of COUP-TFII in mouse NCCs results in markedly shortened and bifurcated tympanic rings, which in turn disturb the caudal direction of external acoustic meatus invagination. However, formation of the manubrium of the malleus (MM) in Wnt1-Cre/+;COUP-TFII flox/flox mice is not perturbed, suggesting that the rostral half of the tympanic ring is sufficient to support proper MM development. Interestingly, we found that loss of COUP-TFII up-regulates Sox9 in the tympanic ring primordium and affects the distribution of preosteoblasts before mesenchymal condensation. Together, our results demonstrate that COUP-TFII plays an essential role in regulating the patterning of the NCC-derived tympanic ring.
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Introduction
Neural crest cells (NCCs) are a multipotent, migratory cell population that is unique to vertebrates and is generated transiently during embryonic development. NCCs originate from neural folds at the dorsal part of the closing neural tube, undergo an epithelial-to-mesenchymal transition, delaminate from the neuroepithelium, and migrate extensively to generate numerous derivatives. According to their emigration position along the anteroposterior axis of the embryo, NCCs can be divided into four main functional domains—cranial, cardiac, vagal and sacral, and trunk—each of which possesses distinct differentiation capacities. Of these, cranial NCCs are capable of differentiating into bone and cartilage tissues and constitute the majority of craniofacial structure1. In addition, the bones of skull develop through endochondral or intramembranous ossification, both of which begin with mesenchymal condensation. During endochondral ossification, condensed mesenchymal cells first differentiate into chondrocytes to form a cartilage intermediate that is subsequently replaced by bone. By contrast, during intramembranous ossification, mesenchymal cells directly differentiate into osteoblasts2.
The Sry-related transcription factor Sox9 and runt-related transcription factor 2 (Runx2/Cbfa1) are crucially involved in skeletal development. Mutations in human SOX9 and RUNX2 genes cause the heritable skeletal disorders campomelic and cleidocranial dysplasia, respectively3,4,5,6. Targeted ablation of Sox9 in mice shows that Sox9 not only is required for promoting the commitment of mesenchymal cells to the chondrogenic cell lineage but also serves as a central player in maintaining the lineage fate and successive differentiation program of growth plate chondrocytes7,8,9. Runx2 is imperative for osteoblast cell fate determination and regulates the expression of many osteoblastic genes. Therefore, Runx2 is indispensable for osteoblast differentiation during both endochondral and intramembranous ossification10,11,12,13. Based on the expression profiles of Sox9 and Runx2 at the mesenchymal condensation stage, a binary molecular code is proposed to predict the identity of skeletal tissues14. Before overt skeletogenesis, both Sox9 and Runx2 are expressed in dual-potential osteochondral progenitors. During intramembranous bone formation, the expression of Sox9 is down-regulated and the expression of Runx2 is up-regulated in mesenchymal condensation. On the contrary, Sox9 expression is up-regulated and Runx2 expression is down-regulated during chondrogenic mesenchymal condensation14,15. Nevertheless, Runx2 expression is subsequently up-regulated, which facilitates later endochondral bone formation through activating chondrocyte maturation and osteoblast specification and differentiation in the perichondrium12,16.
Chicken Ovalbumin Upstream Promoter-Transcription Factor II (COUP-TFII, also known as Nr2f2) is an orphan member of the steroid/thyroid hormone receptor superfamily. Previous studies showed that COUP-TFII can function as a transcriptional repressor through direct binding to COUP-TFII response elements or a transcriptional activator by associating with specific transcriptional factors to regulate their target genes in diverse cellular contexts17,18. COUP-TFII is expressed abundantly during the early embryonic stage. Loss of COUP-TFII results in lethality at embryonic day (E)10 with severe defects in angiogenesis and heart development, demonstrating the crucial role of COUP-TFII during mouse embryogenesis19. Conditional ablation of COUP-TFII further revealed that COUP-TFII makes different contributions to the development of various organs, including cell fate specification in venous and lymphatic vasculature20,21, atria of the heart22, and metanephric mesenchyme23; endocardial and epicardial epithelial-mesenchymal transformation24; differentiation of Leydig cells25; and cell proliferation and apoptosis in granule cell precursors of the cerebellum26. COUP-TFII can also fine-tune the lineage-specific differentiation of mesenchymal stem cells to osteogenic and myogenic lineages at the expense of adipocyte and chondrocyte development, both in vivo and in vitro 27.
Recently, genome-wide epigenomic profiling of chromatin landscapes, gene expression, and cis-element sequence analysis of human embryonic stem cell-derived NCCs revealed that TFAP2A, COUP-TFI, and COUP-TFII simultaneously co-occupy human NCC enhancers and facilitate establishment of a transcriptionally active chromatin enhancer state. The functional annotation of TFAP2A and COUP-TFI/COUP-TFII co-bound regions uncovered a strong association with NC gene expression, cranial NCC-derived structures, and craniofacial anomalies, suggesting that COUP-TFII regulates NC development and craniofacial morphogenesis28,29. However, the in vivo physiological function of COUP-TFII in NC development has not been well studied. Here, we show that COUP-TFII is expressed in NCCs and their derivatives. Targeted ablation of COUP-TFII in premigratory NCCs by Wnt1-Cre transgenic mice30 dramatically shortens the tympanic ring with a bifurcated end. Loss of COUP-TFII up-regulates the expression of Sox9 in the tympanic ring primordium before mesenchymal condensation and affects the distribution of preosteoblasts, uncovering an essential role of COUP-TFII in the patterning of tympanic ring development. Therefore, our study provides new insights into the role of COUP-TFII in NCC-derived intramembranous bone formation.
Results
COUP-TFII is expressed in the NCC-derived subpopulation
To explore whether COUP-TFII plays a physiological role in regulating development of the NCC lineage, we examined the spatiotemporal expression of COUP-TFII. First, we monitored COUP-TFII expression by performing whole-mount X-Gal staining of COUP-TFII Z/+ embryos31, in which the LacZ reporter expression is driven by the COUP-TFII promoter. In E10.5 COUP-TFII Z/+ embryos, X-Gal staining revealed relatively high levels of COUP-TFII expression in several embryonic regions, including the caudal telencephalon, posterior two-thirds of the diencephalon, diencephalon-mesencephalon boundary, eye, cephalic flexure, hindbrain, and craniofacial ganglia (Fig. 1a). This expression pattern recapitulates the expression profile of endogenous COUP-TFII32,33. We also observed moderate X-Gal-positive signals in pharyngeal arches (PAs), especially in the posterior part of PA1 and the anterior part of PA2 (Fig. 1a). Dual immunofluorescence staining of E10.5 Wnt1-Cre/+;R26R mTmG/+ embryonic sections further showed COUP-TFII expression in the cephalic mesenchyme and pharyngeal endoderm as well as in the pharyngeal mesenchyme subpopulation (Fig. 1b and e). This COUP-TFII expression in GFP-positive mesenchymal cells of the PAs confirmed that COUP-TFII is expressed in specific tissues of NCC origin (Fig. 1b–g). Next, we performed whole-mount X-Gal staining of COUP-TFII Z/+ embryos to further characterize the expression of COUP-TFII during craniofacial development. We observed spatiotemporal changes in the distribution of strongly X-Gal-stained cells as development proceeded (Fig. 1h–l), indicating that COUP-TFII exhibits a dynamic expression pattern during craniofacial morphogenesis. Also, we noticed that strongly X-Gal-stained cells located within PAs were gradually confined to the corner of the mouth and showed a C-shaped ring rostral to the ear pinna at E13.5 (Fig. 1l).
Next, to characterize the COUP-TFII expression profile in NCCs and their derivatives, we performed lineage-tracing experiments using Wnt1-Cre/+;COUP-TFII flox/+ mice, in which LacZ reporter expression is under control of the COUP-TFII promoter upon Cre-mediated COUP-TFII deletion (Fig. 1h’–l’). The dynamic distribution of strongly X-Gal-stained cells in Wnt1-Cre/+;COUP-TFII flox/+ embryos was comparable to that of COUP-TFII Z/+ embryos from E10.5 to E13.5. These results show that craniofacial cells with high COUP-TFII expression mainly reside within the NCC lineage during development, and therefore COUP-TFII may play an important role in guiding the development of a specific NCC subpopulation.
Loss of COUP-TFII leads to malformation of the tympanic ring and external acoustic meatus (EAM)
Cranial NCCs and their derivatives can differentiate not only into cranial ganglia and connective tissues but also into bone and cartilage in the head and neck1. Therefore, we stained cranial skeletal preparations with Alcian Blue and Alizarin Red to examine skull architecture (Fig. 2a–b). We observed that the tympanic ring, which develops to provide physical support for the tympanic membrane, was slightly thicker in E15.5 Wnt1-Cre/+;COUP-TFII flox/flox mutants (Fig. 2c’) than in littermate controls (Fig. 2c). From E16.5 onward, the normal C-shaped tympanic ring gradually developed in control embryos (Fig. 2d–g). In contrast, the tympanic ring in Wnt1-Cre/+;COUP-TFII flox/flox mutants was noticeably shortened. As development proceeded, abnormal bifurcation of the tympanic ring was also seen in Wnt1-Cre/+;COUP-TFII flox/flox mutants with full penetrance (Fig. 2d’–g’). Comparative histological analyses further confirmed the shortening and abnormal bifurcation of the tympanic ring in Wnt1-Cre/+;COUP-TFII flox/flox mutants (Fig. 2i–k’).
The tympanic ring directs the invagination of the EAM, which later forms the external ear canal34. The EAM is an ectodermal epithelial cell layer that directly originates from the first pharyngeal cleft. Therefore, we utilized the epithelial cell marker cytokeratin 5 (K5) and osteogenic lineage marker Runx2 to designate the spatial locations of the EAM and tympanic ring, respectively. Dual immunofluorescence analysis of K5 and Runx2 revealed defective morphogenesis of the EAM in COUP-TFII mutants (Fig. 3a–j’). The configuration of EAM invagination in Wnt1-Cre/+;COUP-TFII flox/flox mutants was different from that of littermate controls (Fig. 3c and c’). In the presence of the tympanic ring, the extremity of EAM invagination was directed toward the position of the tympanic ring in both control and mutant embryos (Fig. 3f–j and f’–h’). However, in the caudal part, Wnt1-Cre/+;COUP-TFII flox/flox mutants exhibited a misalignment of EAM invagination (Fig. 3i’ and j’), which was a likely consequence of the premature ending and bifurcation of the mutant tympanic ring. Proper EAM invagination is crucial for coordinating the development and positioning of the manubrium of the malleus (MM)34. Therefore, it was surprising that the anomalies in the tympanic ring and EAM invagination of Wnt1-Cre/+;COUP-TFII flox/flox mutants did not perturb the coordinated development of the MM (compare Fig. 3k’ with k), indicating that the rostral half of the tympanic ring is sufficient to guide MM formation.
Loss of COUP-TFII up-regulates Sox9 in the tympanic ring primordium
Sox9 expression is progressively down-regulated in the osteogenic cell compartment during intramembranous ossification, indicating that Sox9 may be involved in incipient intramembranous bone formation35,36. However, the role of Sox9 in the development of intramembranous bones remains poorly understood. In mice, the primordium for the tympanic ring becomes apparent by E13.5 as a condensation ventral to the first pharyngeal cleft lateral to Meckel’s cartilage37. To investigate whether Sox9 contributes to malformation of the tympanic ring in Wnt1-Cre/+;COUP-TFII flox/flox mutants, we first performed COUP-TFII, β-galactosidase (β-gal), and Sox9 immunostaining of E13.5 head sections. COUP-TFII was detected in almost all cranial mesenchyme but not in the EAM around the otic region of COUP-TFII flox/flox control mice. Compared with COUP-TFII flox/flox control embryos (Fig. 4a), COUP-TFII levels were moderately decreased and nearly undetectable in Wnt1-Cre/+;COUP-TFII flox/+ (Fig. 4a’) and Wnt1-Cre/+;COUP-TFII flox/flox (Fig. 4a”) embryos, respectively. The profiles of Wnt1-Cre-mediated COUP-TFII ablation, as visualized by anti-β-gal staining in cranial mesenchymes around the EAM, were indistinguishable between Wnt1-Cre/+;COUP-TFII flox/+ (Fig. 4b’) and Wnt1-Cre/+;COUP-TFII flox/flox (Fig. 4b”) embryos except that the intensity of β-gal staining (Fig. 4b–b”) was inversely correlated with levels of COUP-TFII. Interestingly, up-regulation of Sox9 was observed in the specific subset of mesenchymal cells medial to the EAM and lateral to Meckel’s cartilage, where the future tympanic ring is situated, in E13.5 Wnt1-Cre/+;COUP-TFII flox/+ (Fig. 4c’) and Wnt1-Cre/+;COUP-TFII flox/flox (Fig. 4c”) embryos. Moreover, we detected Sox9 up-regulation in the corresponding β-gal-expressing region. Notably, no obvious cellular condensation was observed in this Sox9-expressing population at this stage (Fig. 4e–e”). To ensure equivalent fluorescent intensity of β-gal-expressing cells in control and mutant embryos, Wnt1-Cre/+;COUP-TFII flox/+ mice were mated with COUP-TFII +/− mice to generate Wnt1-Cre/+;COUP-TFII flox/+ and Wnt1-Cre/+;COUP-TFII flox/− mice that contained only one LacZ allele. Additional immunohistochemical analyses showed comparable expression patterns of β-gal between Wnt1-Cre/+;COUP-TFII flox/+ and Wnt1-Cre/+;COUP-TFII flox/− embryos (Supplementary Fig. S1b-b”). We also confirmed that Sox9 expression was up-regulated in the subpopulation of evenly distributed β-gal-positive mesenchyme in Wnt1-Cre/+;COUP-TFII flox/− mutants (Supplementary Fig. S1). These results indicate that loss of COUP-TFII does not interrupt the migration and localization of NC lineage cells to the otic region but results in Sox9 up-regulation in the subset of COUP-TFII-expressing NCC-derived mesenchyme that may give rise to the tympanic ring before mesenchymal condensation. The differential expression of Sox9 in tympanic ring became evident at E14.5 (Supplementary Fig. S2a-e’). We observed consistent up-regulation of Sox9 in Wnt1-Cre/+;COUP-TFII flox/flox mutants compared with control mice, and those Sox9-expressing mesenchymal cells in mutants also exhibited abnormal expansion with a bifurcated end (compare Supplementary Fig. S2a’–b’ with a–b). In control embryo, Sox9-expressing mesenchymal cells were detected in all examined otic region. However, in Wnt1-Cre/+;COUP-TFII flox/flox mutants, Sox9-expressing mesenchymal cells were not seen in the caudalmost part of examined region (Supplementary Fig. S2e’ and e). Consistent with the previous study showing that COUP-TFII expression is gradually down-regulated during osteoblast differentiation38, we noticed that there was a much weaker β-gal signal in the corresponding Sox9-expressing region at E14.5 (Supplementary Fig. S2f–j’), which is in contrast to the uniform β-gal expression in cranial mesenchyme around the E13.5 otic region. These results further suggest that Sox9 is expressed in the primordium for the future tympanic ring.
At a later stage (E16.5) when the bony structure of the tympanic ring can be clearly identified by histological analysis, the level of Sox9 expression was lower in the bony part of the tympanic ring than in the surrounding mesenchyme in both control and mutant embryos (Fig. 5a–j’), consistent with previous studies reporting the down-regulation of Sox9 expression during intramembranous ossification35,36. We also noticed that high levels of Sox9 were detected in the mesenchyme around the caudal end of developing tympanic ring in both control and COUP-TFII mutant embryos (control – Fig. 5i and j; mutant – Fig. 5h’ and i’). Nevertheless, the Sox9 expression in Wnt1-Cre/+;COUP-TFII flox/flox mutants was consistently higher in the surrounding mesenchyme of the rostral part of tympanic ring than that of the control mice (compare Fig. 5f–h with f’–g’). The master regulator of osteogenesis, Runx2, was highly expressed in the bony part of the tympanic ring, and more moderate levels were observed in Sox9-positive surrounding mesenchymal cells in both control and Wnt1-Cre/+;COUP-TFII flox/flox mutant mice (Fig. 5k–o’). These results suggest that COUP-TFII may coordinate the tympanic ring development by inhibiting Sox9 expression in the osteogenic mesenchyme. Given that Sox9 is required for determining chondrogenic cell lineage and is sufficient for cartilage formation8,14,39,40, we next investigated whether malformations of the tympanic ring in Wnt1-Cre/+;COUP-TFII flox/flox mutants result from inaccurate cell fate decision (i.e., chondrogenic cell fate) due to abnormal up-regulation of Sox9. However, skeletal preparations revealed that corresponding regions of the Alizarin Red-stained tympanic ring showed no Alcian Blue (i.e., cartilage) staining in either E18.5 controls or Wnt1-Cre/+;COUP-TFII flox/flox mutants (Fig. 5p–q’). Thus, aberrant Sox9 up-regulation did not result in the diversion of osteogenic mesenchymal cells toward cartilage differentiation. Collectively, our findings indicate that COUP-TFII-null mesenchymal cells are able to condense, differentiate into osteoblasts, and form a truncated tympanic ring in Wnt1-Cre/+;COUP-TFII flox/flox mutants through intramembranous ossification.
COUP-TFII governs the distribution of preosteoblasts during tympanic ring development
Mesenchymal condensation is a crucial stage for skeletal patterning during bone development. Therefore, we analyzed mesenchymal condensation to verify whether the COUP-TFII-null mesenchyme exhibits impaired skeletal patterning for the future tympanic ring. Tenascin-C, a glycoprotein of extracellular matrix components, is involved in establishing the condensation boundary of the osteogenic population from non-skeletogenic mesenchymes41,42,43. At E13.5, tenascin-C expression was detected in the condensed mesenchyme as evidenced by cell aggregation but not in the non-condensed mesenchyme of the developing tympanic ring in both control and mutant embryos (Fig. 6a–e’). Loss of COUP-TFII in NCCs did not affect the level of tenascin-C expression, but tenascin-C-positive condensed mesenchyme within the developing tympanic ring of Wnt1-Cre/+;COUP-TFII flox/flox mutants did not extend caudally as far as the condensed mesenchyme did in control mice (compare Fig. 6c’ with c). This result revealed that the abnormal shortening of condensed mesenchymal structure contributes to anomalies of the tympanic ring in Wnt1-Cre/+;COUP-TFII flox/flox mutants. Similarly, expression of the osteogenic markers Runx2 and Osterix (Osx) at the single-cell level were comparable between the corresponding regions within the developing tympanic rings of control and COUP-TFII mutant embryos. However, both Runx2- and Osx-expressing mesenchymal cells were not detected in the caudal region of Wnt1-Cre/+;COUP-TFII flox/flox mutants, while such cells could easily be seen in the counterpart of control mice (Fig. 6f–o’). According to previous studies, specified preosteoblasts express Runx2, and at a later stage, preosteoblast committed to an osteoblast lineage express both Runx2 and Osx44. Therefore, these results revealed that, instead of extending into more caudal region as in control mice, the distributions of both specified and committed preostroblasts were rostrally restricted in Wnt1-Cre/+;COUP-TFII flox/flox mutants. Interestingly, we noted that Runx2- and Osx-positive signals were detected not only in condensed mesenchyme but also in non-condensed mesenchyme at the caudal part of the developing tympanic ring (Fig. 6a–o’). Thus, the distributions of Runx2- and Osx-expressing cells were much broader than that of tenascin-C-expressing cells, which indicates that, before mesenchymal condensation, these Runx2- and Osx-expressing preosteoblasts already exist during tympanic ring development. Taken together, our results suggest that loss of COUP-TFII results in aberrant up-regulation of Sox9 in the subpopulation of NCC-derived mesenchyme and affects the distribution of preosteoblasts, which in turn leads to improper pattern of mesenchymal condensation and consequently truncated tympanic ring formation.
Discussion
The work presented here unveils the differential expression of COUP-TFII and its biological function in cranial NCCs and their derivatives in vivo. We showed that targeted inactivation of COUP-TFII in the NCC lineage leads to tympanic ring abnormalities. Genetic evidence showed that COUP-TFII deficiency results in the aberrant expression/regulation of Sox9 in the primordium of the tympanic ring and affects the distribution of preosteoblasts before mesenchymal condensation.
In vivo fate mapping and comparative histological analyses showed that loss of COUP-TFII affects neither NCC migration from neural folds to the ear region nor survival during this period. Before mesenchymal condensation, we detected COUP-TFII loss-of-function and increased Sox9 protein levels in the mesenchyme that presumably gives rise to the tympanic ring in E13.5 Wnt1-Cre/+;COUP-TFII flox/flox mutants, suggesting that COUP-TFII may negatively regulate Sox9 expression in the osteogenic mesenchyme. This result stands in contrast to the previous finding showing that COUP-TFII is recruited to the Sox9 regulatory region by Sp1 to activate the expression of Sox9, leading to the chondrogenic commitment of mesenchymal precursors27. The underlying basis for the inverse relationship between COUP-TFII and Sox9 during osteogenesis versus chondrogenesis is currently not clear. One possibility is that COUP-TFII interacts with other transcription factors to cooperatively inhibit Sox9 expression, whereas another possibility is that Sox9 is an indirect target of COUP-TFII-mediated repression. Future studies are needed to delineate how COUP-TFII regulates Sox9 expression in osteogenesis. In addition, COUP-TFII physically interacts with Runx2 and interferes with its binding on the target promoter, which inhibits the transcriptional activity, but not the level of Runx2, in directed osteoblast differentiation27,38. However, we found that loss of COUP-TFII does not affect the expression level of Runx2 or its direct targets, Osx and osteocalcin (OC) (Supplementary Fig. S3), suggesting that COUP-TFII does not regulate Runx2 transactivity during osteoblast differentiation in the developing tympanic ring.
We detected Runx2- and Osx-positive signals, which indicate commitment of preosteoblasts to an osteoblast lineage, in dispersed mesenchyme, as well as in condensed mesenchyme (i.e., tenascin-C-expressing cells or the cell aggregation in tissues), in the developing tympanic ring, which reveals that preosteoblast commitment precedes mesenchymal condensation during tympanic ring development. A similar process has been observed during the development of the chicken mandible45, which also occurs through intramembranous ossification. Therefore, our findings provide further evidence supporting that osteogenesis is different from chondrogenesis, in which mesenchymal condensation triggers prechondroblast commitment46,47.
In skeletal development, Sox9 and Runx2 are the major transcription factors for chondrogenesis and osteogenesis, respectively. The spatiotemporal overlap of Sox9 and Runx2 expression unveils lineage-specific roles in orchestrating transcription of downstream targets during bone formation. Loss of Sox9 in NCCs and growth plate result in ectopic expression of osteoblast marker genes, such as Runx2, Osx and Col1a1, in the presumptive cartilage existing in wild-type embryos and differentiated growth plate chondrocytes, respectively8,9. In addition to directly activating the expression of major cartilage-specific extracellular matrix genes, Sox9 is also capable of suppressing Runx2 expression to specify and maintain the chondrogenic lineage fate48,49,50. By contrast, the role of Sox9 in osteogenesis is not well understood. Sox9 expression is gradually down-regulated during intramembranous bone formation35,36. In cranial skeletogenesis, misexpression of Sox9 results in down-regulation of Runx2 in chondrocytes of the ceratobranchial cartilage, whereas the expression of Runx2 in osteoblasts and osteogenic mesenchymal condensation are not disrupted in the intramembranous surangular bone14. Further mechanistic investigation showed that Sox9 suppresses the transactivity of Runx2 in the established osteoblast lineage, which hinders osteoblast differentiation51. Our study provides additional in vivo evidence that the regulation of Runx2 by Sox9 during osteogenesis is different from that during chondrogenesis. Despite the shortened primordium of the tympanic ring in Wnt1-Cre/+;COUP-TFII flox/flox mutants, abnormal Sox9 up-regulation did not affect the expression of Runx2, and Runx2-positive cells still resided within their anatomical locations, consistent with previous observations in surangular bone14. Notably, the expression levels of Osx and OC, the direct targets of Runx2, were not altered, indicating that abnormally increased Sox9 in the Wnt1-Cre/+;COUP-TFII flox/flox tympanic ring primordium does not inhibit the transactivity of Runx2. However, we did not rule out the possibility that the level of Sox9 might not have been sufficient to inhibit Runx2 transactivity in mutants. The inconsistencies among studies may also be due to different experimental approaches under varying physiological and cellular contexts.
Morphogenesis of the tympanic ring, EAM, and MM are spatially and temporally associated37,52. This close relationship is also observed through clinical experience with humans53,54. Analyses of genetically modified (Gsc −/− and Prx1 −/− mutants) and retinoic acid-treated mouse embryos support the deduction that the tympanic ring is essential for inducing the invagination of the first pharyngeal cleft to form the EAM, which then provides signals that act on the underling mesenchymal cells to coordinate proper MM development34,52,55,56,57,58. It is noteworthy that the severity of tympanic ring anomaly is tightly associated with the level of MM deformity. Concurrent disappearance of the tympanic ring and MM is also found in endothelin A receptor-deficient mice59,60. Both Gas1-deficient and Tshz1-deficient mice have shorter and thicker tympanic rings accompanied by the absence of a MM61,62. Bapx1 −/− embryos show relatively normal MM formation but exhibit hypoplasia (i.e., thinness) of the tympanic ring only in the anterior/rostral part63. Although EAM development has not been clearly described in every aforementioned mutant, studies consistently indicate that the MM forms in a coordinated manner alongside tympanic ring development. In this study, we found that the loss of COUP-TFII in the NCC lineage results in a shortened and thicker tympanic ring. Although this truncated tympanic ring was able to induce and guide the morphogenesis of EAM invagination, the extremity of caudal EAM invagination went astray. However, the MM was well developed and properly positioned in Wnt1-Cre/+;COUP-TFII flox/flox mutants, suggesting that the rostral half of the tympanic ring—rather than the entire structure—is sufficient to coordinate the development and positioning of the MM. Nevertheless, this hypothesis is inconsistent with phenotypes observed in Tshz1 −/− mutants. The morphologies of the shortened tympanic rings in Wnt1-Cre/+;COUP-TFII flox/flox and Tshz1 −/− mutants are similar to each other and to that of the rostral half of the control tympanic ring; however, MM development differs between these two mutants. Coré et al. showed that Tshz1 is expressed in the mesenchyme surrounding the malleal primordium, implying that Tshz1 may play a cell-autonomous role in MM development. By contrast, down-regulation of COUP-TFII in the Sox9-positive developing malleus and neighboring mesenchyme was noted in control embryos from E12.5 (Supplementary Fig. S4). Therefore, the discrepancy in MM development between Wnt1-Cre/+;COUP-TFII flox/flox and Tshz1 −/− mutants could result from a difference between the cell-autonomous roles of COUP-TFII and Tshz1.
In summary, our study establishes that COUP-TFII is an essential transcription factor in NCCs and their derivatives for tympanic ring development in vivo. Our results provide new insights into the differential roles of COUP-TFII in fine-tuning the expression of Sox9 and regulating the distribution of preosteoblasts at the beginning of intramembranous bone development. As previous studies report that COUP-TFII can specify the lineage commitment of mesenchymal stem/stromal cells (MSCs), prospective studies aimed at understanding the precise spatiotemporal control and plasticity of MSCs in vivo will be of great benefit to future MSCs-based therapy.
Materials and Methods
Mice
COUP-TFII +/− 19, COUP-TFII flox/flox, and COUP-TFII Z/+ 31 mice were kindly provided by Dr. Sophia Y. Tsai, Baylor College of Medicine, Houston, Texas. To inactivate COUP-TFII expression in the NCC lineage, Wnt1-Cre transgenic mice (Tg(Wnt1-Cre)11Rth)30 were crossed with COUP-TFII flox/flox mice. Double-heterozygous Wnt1-Cre/+;COUP-TFII flox/+ mice were then mated with COUP-TFII flox/flox or COUP-TFII +/− mice to generate Wnt1-Cre/+;COUP-TFII flox/flox or Wnt1-Cre/+;COUP-TFII flox/− mice, respectively. For lineage-tracing experiments, the Cre reporter mouse line R26R mTmG/+ 64 was crossed with Wnt1-Cre transgenic mice to characterize the distribution of neural crest derivatives as described previously65. The animal experiments were approved by the Institutional Animal Care and Use Committee (IACUC) of National Yang-Ming University, Taiwan. The animal care and experimental procedures were performed in accordance with the Guidelines of the IACUC of National Yang-Ming University, Taiwan.
Whole-mount X-Gal staining
Embryos from timed-pregnant mice were collected and fixed in 2% paraformaldehyde (PFA; Electron Microscopy Science)/1 × phosphate-buffered saline (PBS), pH 7.3 for 10–15 min at 4 °C. Embryos were then incubated in X-Gal staining solution at 30 °C until appropriate color development and post-fixed in 4% PFA/PBS at 4 °C overnight. Detailed materials and procedures were described previously24.
Histological and immunohistochemical staining
Histological and immunohistochemical experiments were performed as described previously24,66. Antibodies against β-gal (Chemicon, AB1211, 1:1200), COUP-TFII (Perseus Proteomics, PP-H7147, 1:1000), GFP (Genetex, GTX13970, 1:100), K5 (Abcam, ab24647, 1:125), Osx (Abcam, ab22552, 1:3000), Runx2 (Santa Cruz Biotechnology, sc10758 [M-70], 1:1000), Sox9 (Santa Cruz Biotechnology, sc20095 [H-90], 1:150), and tenascin-C (Millipore, AB19013, 1:3000) were used.
Skeletal preparations
Skeletal staining was performed as described previously65 with slight modifications. Briefly, embryo heads were carefully skin-peeled and fixed in 95% ethanol followed by degreasing with acetone. Cartilage and bone were visualized by staining with 0.05% Alcian Blue 8GX (Sigma-Aldrich) in acid ethanol and 0.01% Alizarin Red S (Alfa Aesar) in 1% KOH, respectively. Soft tissues were cleared by 1% KOH. For single Alcian Blue staining, the step for Alizarin Red S staining was omitted, and the samples were directly immersed in 1% KOH.
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Acknowledgements
We would like to thank Dr. Sophia Y. Tsai for kindly providing the COUP-TFII flox/flox, COUP-TFII Z/+ and COUP-TFII +/− mice used in this study and for critical reading and helpful comments on the manuscript. We also thank Dr. Yu-Ting Yan for helpful discussions on the manuscript. This work was supported by the Ministry of Science and Technology of Taiwan (grant numbers MOST103-2320-B-010-029-, MOST104-2320-B-010-002- and MOST105-2320-B-010-027- to L.-R.Y.); and the Ministry of Education, Aim for the Top University Plan (grant numbers 104AC-T503 and 105AC-T503 to L.-R.Y.).
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Conceptualization: W.-H.H., C.-M.C., L.-R.Y.; Investigation: W.-H.H.; Writing - original draft preparation: W.-H.H., L.-R.Y.; Writing - review and editing: C.-M.C., L.-R.Y.; Resources: C.-M.C., L.-R.Y.; Supervision: L.-R.Y.
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Hsu, WH., Chen, CM. & You, LR. COUP-TFII is required for morphogenesis of the neural crest-derived tympanic ring. Sci Rep 7, 12386 (2017). https://doi.org/10.1038/s41598-017-12665-0
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DOI: https://doi.org/10.1038/s41598-017-12665-0
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