The overall similarity of the skull shape of some dog breeds with that of juvenile wolves begs the question if and how ontogenetic changes such as paedomorphosis (evolutionary juvenilisation) played a role in domestication. Here we test for changes in patterns of development and growth during dog domestication. We present the first geometric morphometric study using ontogenetic series of dog and wolf crania, and samples of dogs with relatively ancestral morphology and from different time periods. We show that patterns of juvenile-to-adult morphological change are largely similar in wolves and domestic dogs, but differ in two ways. First, dog skulls show unique (neomorphic) features already shortly after birth, and these features persist throughout postnatal ontogeny. Second, at any given age, juvenile dogs exhibit skull shapes that resemble those of consistently younger wolves, even in dog breeds that do not exhibit a ‘juvenilized’ morphology as adults. These patterns exemplify the complex nature of evolutionary changes during dog domestication: the cranial morphology of adult dogs cannot simply be explained as either neomorphic or paedomorphic. The key to our understanding of dog domestication may lie in a closer comparative examination of developmental phases.
Investigating how novel variation of domesticated forms is generated through changes in development and growth brings new insights into the micro-evolutionary processes of domestication. How do new morphological and behavioural peculiarities of domesticated species arise1,2,3,4? The retention of juvenile characteristics of wolves (Canis lupus) into the adulthood of domestic dogs has been suggested to describe the anatomical and behavioural disparity between the ancestor and the domesticated form5,6,7,8,9,10,11. Morphologically, the relatively short face and broad skull of domestic dogs have been interpreted as a paedomorphic pattern5,6,7,8,9,10. The size relationships between major portions of the skull during growth, coupled with a body size decrease due to domestication, could contribute to a juvenilized skull shape in domestic dogs12: the neurocranium scales negatively allometrically and the facial region positively allometrically with body size, thereby leading to a relatively short snout and proportionally large braincase in some domestic dogs. However, the paedomorphosis hypothesis has been challenged on various grounds12,13,14,15. In particular, a recent study revealed no resemblance between adult cranial shape of modern breeds and neither adult or juvenile wolves15. This led to the conclusion that dog cranial shape is neomorphic, i.e., showing novel, taxon-specific features that cannot simply be explained as paedomorphic (i.e., juvenilized) variants of the wolf morphology.
Neomorphosis and paedomorphosis as patterns of evolutionary-developmental differentiation between groups are not mutually exclusive but likely act in concert to generate taxon-specific morphology. Neomorphosis largely corresponds to the notion of heterotopy (originally coined by Haeckel16), which denotes modification of spatial/structural aspects of the ancestral developmental program leading to new morphology. Paedomorphosis, on the other hand, results from heterochrony, which denotes modification of temporal characteristics (onset, rate, and cessation) of the ancestral developmental program. Both empirical and theoretical data indicate that the evolutionary diversification of closely related taxa typically results from a combination of spatial and temporal modifications of the ancestor’s developmental program17. In hominoids, for example, neonates already exhibit distinct, taxon-specific cranial shapes, indicating prenatal neomorphosis18. Postnatal cranial ontogeny, on the other hand, largely unfolds along a trajectory shared by all taxa, while taxon-specific differences arise through heterochronic shifts along the trajectory18.
Here, we assess the relative contribution of neomorphic and paedomorphic modifications during dog domestication. Obviously, this can only be done by comparing patterns of ontogeny in wolves (the ancestral form) and dogs (‘descendant’ forms resulting from domestication). In this study, we test the neomorphosis and paedomorphosis hypotheses taking into account two aspects which have not yet been considered in similar studies: (1) comparing cranial ontogeny of wolves and dogs including juveniles and adults of both groups; (2) taking into account that different selection regimes may have acted on dog crania during the course of domestication. To track group-specific ontogenetic trajectories, we document cranial growth and development from early postnatal life to adulthood in cross-sectional ontogenetic series, using methods of geometric mophometrics19,20 and phenotypic trajectory analysis21.
Phenotypic trajectory analysis is a method based on geometric morphometrics22, which provides a straightforward operationalization of the concepts of heterotopic (neomorphic) and heterochronic (e.g., paedomorphic) modification of ontogenetic trajectories23,24. In our case, each group’s (wolf, dog groups and breeds) pattern of three-dimensional cranial shape change from juveniles to adults is characterized by an ontogenetic trajectory through multidimensional shape space. Provided that group-specific trajectories are fairly linear, their directions can be compared statistically25. Divergence of dog relative to wolf trajectories indicates heterotopic modification. Collinearity or parallelism of wolf and dog trajectories in shape space is a precondition to analyse heterochronic modifications. These manifest themselves as shifts of stage-specific locations (such as neonate, adult) along the shared trajectory direction.
In comparing skull shape modifications during domestication, it is crucial to take into account that past and recent processes of domestication and selection may be fundamentally different. In its early phase, the domestication process was likely driven by (relaxed) natural selection in an anthropogenic environment and unintentional artificial selection, probably for tameness26,27,28. In contrast, cranial proportions of modern domestic dog breeds are clearly the effect of intentional artificial selection driven by aesthetic or functional requirements (e.g., refs29,30). One example is brachycephaly and airorhynchy, which describe a short skull relative to its width and a dorsally rotated rostrum in e.g., bulldogs31. Although initially described as the retention of juvenile characters (paedomorphosis)12, such extreme morphology is clearly neomorphic15 and likely related to alterations in the branchial arch developmental system32,33. To avoid the confounding effects of modern breeding regimes, we investigate dog breeds that are either relatively close to the wolf skull morphology (mesaticephalic), or were likely less subject to a strong artificial selection regime, such as pre-modern and archaeological domestic morphotypes.
‘Modern’ breeds are recognized by kennel clubs and have been subject to exhaustive breeding regimes with strict aesthetic requirements and closed bloodlines during the last 150 years or so34. This group is represented here by one breed that is wolf-like in appearance and size, the German shepherd (Table 1). ‘Premodern’ domestic dogs are defined here as populations that are geographically and/or culturally isolated from the modern domestic dog breeds and occupy well-supported basal positions on molecular phylogenetic trees34,35,36. This group is represented by the Afghan hound, Akita, Australian dingo, and New Guinea singing dog (Table 1). The latter two are feral37 and likely submitted to less intense artificial selection and more intense natural selection38. The premodern sample also includes a group from the early 19th century, when dogs were bred and classified according to their function and selection for aesthetics traits was of minor importance. This study contains one such type of dog, the pointing dog (‘Hühnerhund’), which was used to track down prey during a hunt (Table 1). ‘Archaeological’ domestic dogs represent prehistoric (Neolithic) and proto-historic (Iron Age) dogs from Switzerland (Table 1)39; these reflect the most ancestral forms in our dataset.
We use this sample to address two questions: 1) when during ontogeny do the unique (neomorphic) features of the cranium of dogs appear? 2) Taking into account neomorphic differences between wolf and dogs, is there evidence for a paedomorphic pattern of cranial development in domestic dogs compared to wolves?
Figure 1 visualizes patterns of cranial shape variation along the first two principal components (PCs) of shape space. PC1 explains 53.49% of the total variation and is associated with a relative elongation and narrowing of the rostrum and a dorsoventral flattening of the braincase (Fig. 1). PC2 (7.86%) captures variation in rostrum width, neurocranial height, and rotation of the rostrum relative to the cranial base (Fig. 1). PC1 and 2 are further both associated with variation in the caudal protrusion of the external occipital protuberance (Fig. 1). The ontogenetic trajectories of cranial shape change in wolf and domestic dog extend mainly along PC1, whereas differences among groups/breeds of domestic dogs are mainly along PC2 (Fig. 1 ). However, there is overlap between groups along PC1 and PC2.
Statistical comparisons of between-group morphological similarity (assessed by MANOVAs and quantified by Mahalanobis distances, Supplementary Information Table S1) show that the cranial morphology of domestic dogs not only differs from that of wolves during adulthood, but already in the juvenile forms (Fig. 2, Supplementary Information Table S1). The New Guinea singing dog is morphologically more distant from the wolf than most other domestic groups, including the modern German shepherd (Figs 1, 2, Supplementary Information Table S1). The archaeological dogs, pointing dogs and Australian dingos cluster together and are relatively closer to the wolves than the other domestic dogs (Fig. 2, Supplementary Information Table S1).
Phenotypic trajectory analysis reveals that no difference exists between the direction of the wolf ontogenetic trajectory and that of any of the domestic dog groups/breeds (Table 2). However, the trajectories of wolf and dog breeds show differences in their position in morphospace (indicative for heterotopy), as well as in their length (indicative for heterochrony, Fig. 3, Table 2). Therefore, trajectories are largely parallel to each other and group-specific, heterotopic differences arise early during development, and persist into adulthood (Fig. 3). Given that group-specific trajectories are largely parallel to each other, it is possible to compare them in terms of heterochronic modification of their shared ancestral (wolf) trajectory. Compared to adult wolves, the adults of some dog groups are less advanced along the shared trajectory vector, i.e., have clearly paedomorphic crania (e.g., Australian dingo; Figs 1, 3). Others have similarly advanced cranial shapes (German shepherd) or even tend to be more advanced than wolves (peramorphic; Afghan hound; Figs 1, 3). For comparisons of trajectory length, the Akita sample is crucial because the youngest known-age specimens are of similar age as the youngest known-age wolf specimens (12 and 13 days, respectively, Fig. 3). The juvenile-to-adult trajectory of the Akita is slightly longer than that of the wolf (Fig. 3, Table 2, Supplementary Information Fig. S1). Most significantly, Akita juveniles have clearly more paedomorphic cranial shapes than wolf juveniles of the same age; Supplementary Information Fig. S1).
There is an overall similar direction of postnatal cranial ontogenetic trajectories in wolves and domestic dogs (Fig. 3, Table 2), implying that spatial patterns of cranial shape change are similar in these groups. We infer that the postnatal cranial ontogenies of wolf and domestic dogs follow a shared ancestral pattern of morphological change, but with heterochronic changes. On the other hand, skull shapes of domestic dogs and wolves are different already early after birth (Figs 1, 2, 3, Supplementary Information Table S1). Thus, we deduce that the group-specific neomorphic deviation of domestic dog crania from wolf crania likely reflects neomorphic processes during pre- and/or perinatal development. The ontogenetic timing of artificial selection might have an impact on the observed neomorphy early during postnatal ontogeny, as selection on domesticated animals is essentially a two-stage process: ‘culling’ of puppies for non-reproduction, followed by selection of adults for reproduction. Both processes might have had natural and cultural (intentional breeding) components.
In sum, our results provide support for both the neomorphosis hypothesis12,13,14,15 and the paedomorphosis hypothesis5,6,7,8,9,10, but at different stages of ontogeny. These patterns of ontogenetic modification are apparently not the result of recently applied artificial selection related to modern breed formation but were probably already present in early phases of the domestication process as a result of (relaxed) natural selection in an anthropogenic environment and/or ancient artificial selection (for tameness and/or increased reproduction rates)26,28.
While it is generally assumed that the cranial morphology of adult dogs is a good indicator of ongoing domestication and related (artificial) selection regimes40,41,42,43, our ontogenetic data allow us to critically assess this supposition. Adult archaeological dogs, pointing dogs, and Australian dingos, with a supposedly more ancestral skull conformation, are indeed more similar to adult wolves than groups which have been artificially selected more intensively, such as Akita, Afghan hound, and German shepherd (Figs 1, 2, Supplementary Information Table S1). On the other hand, adult New Guinea singing dogs, which are unlikely to have been subject to strong artificial selection, occupy positions in cranial shape space that are more distant from adults of wolves than any other domestic dog group/breed (Figs 1, 2). We conclude that adult skull shape alone cannot be used as a reliable indicator of paedomorphic processes during dog domestication, nor of specific domestication-related selection regimes. (The term ‘paedomorphosis’ is typically only used to compare adults – i.e., endpoints of ontogenetic trajectories – of ancestral and descendant groups. Here we use the term to describe ancestor-descendant differences between same-age groups at any point along the ontogenetic trajectory). However, our data on juveniles indicate a previously undescribed mechanism of paedomorphic shift during early ontogeny that might be relevant to understand dog domestication. As shown in Figure 3 and Supplementary Information Figure S1, known-age domestic dog juveniles are consistently more paedomorphic than wolves at the same age. Gestation length is relatively constant in different-sized domestic dogs44,45 and remarkably invariant between the domestic dog (64–66 days46) and the wolf (60–65 days47). In view of these data, the developmental shifts documented here (Fig. 3) likely indicate a pattern of deceleration of the rate of pre/perinatal skull development in dogs compared to wolves, resulting in a paedomorphic shift during early postnatal development. Depending on the rate of postnatal ontogeny, paedomorphic neonates might result in paedomorphic adults, in wolf-like adults (neutral in terms of heterochrony), or even in peramorphic adults. The known-age juvenile subsample available for this study is comparatively small, and larger samples will be required to substantiate the hypothesis of early paedomorphic shifts.
A trend toward more altricial newborns would present the ideal neurological substrate for the development of behavioral features such as tameness. It has been hypothesized that a low degree of organ differentiation at birth may lead to a more flexible reaction of the development to environmental conditions after birth (e.g., through artificial selection) because organogenesis (e.g., bone formation, brain development) is still underway48 and the development of the brain might be more sensitive to environmental influences (such as social context). Indeed, it has been shown that in silver foxes that had been selected for tameness over 45 generations, and in domestic dogs the period of socialization begins earlier than in their non-tamed/wild ancestors (see ref.49 for a review).
The ‘early paedomorphosis’ hypothesis offers interesting comparative perspectives on the proximate, developmental patterns of domestication. Humans compared to our closest relatives, the great apes, are characterized by secondary altriciality (helpless, fetal-like neonates). A trend toward secondary altriciality has long been postulated to be a prerequisite of early cognitive and social development in humans and their fossil relatives50, as early brain development is strongly influenced by an individual’s social environment.
To conclude, we found that the process of dog domestication involved evolutionary developmental modifications that resulted in a combination of neomorphic and paedomorphic features of cranial morphology. Hypotheses about paedomorphic shifts cannot be answered by comparing adult morphotypes alone, but require complete comparative ontogenetic series. Ideally, investigations on cranial shape changes during perinatal development of domestic dogs and wolves will further elucidate the significance of early developmental shifts during domestication.
Materials and Methods
Specimens, landmarks, and age stages
Ontogenetic series of crania of 110 specimens were used in this study: 39 wolves (15 juveniles, 24 adults) and 71 domestic dogs (19 juveniles, 52 adults) (Table 1). The specimens are housed in the Zoologisches Institut/Populationsgenetik (former Institut für Haustierkunde), Christian-Albrechts-Universität zu Kiel, Germany (I.f.H.); Naturhistorisches Museum Bern, Switzerland (NMBE); Naturhistoriska Riksmuseet, Stockholm, Sweden (NRM). One CT-scanned specimens is housed in the Zoologisches Institut und Museum der Universität Zürich, Switzerland (ZMUZH) and three CT-scanned specimens were contributed from the Tierpark Bern, Dählhölzli & Bärenpark, Switzerland (TBDB). No specimen was killed for this study and no live specimens were used.
The three-dimensional coordinates of 34 landmarks on the left and the right side of the skull (Fig. 4, Table 3) were captured with a MicroScribe MX 3D digitizer (Solution Technologies, Inc.). Additionally, one specimen was scanned with a micro-CT (XtremeCT II, SCANCO Medical AG, Brüttisellen, Switzerland) and three specimens were scanned with a medical CT (SOMATOM Definition Flash, Siemens Healthcare, Forchheim, Germany). Isosurfaces were subsequently extracted with Avizo version 6.2.1 or VGStudio MAX version 2.2.5 and landmarks were captured with MeshLab version 1.3.3. The choice of landmarks was based on their recognizability in all age stages. The ventral and the dorsal aspects of the crania that have been digitized with a MicroScribe were combined into one set of coordinates for the entire skull through digitizing three landmarks from both aspects. Forty-nine crania were digitized twice to account for random errors in individual measurements during digitization. To determine the amount of measurement error between the two replicates in every specimen, Procrustes ANOVA was calculated51. The amount of shape variation due to digitizing error in the skull is 58–117 times smaller than any biological variation (among individuals and/or between the two sides of the skull) and is thus considered negligible. Captive wolves were only considered if they were less than one month old because skull shape can be greatly altered in captive specimens52,53. However, these changes were not considered extensive in very young specimens. All scanning data of the specimens using the medical CT are available in MorphoMuseuM54.
A few specimens were not complete, i.e., not all landmark positions were measurable. There were 21 specimens with at least one missing landmark. The maximum number of missing landmarks in one specimen was 4 (a juvenile Australian dingo). The missing landmarks were estimated using the ‘estimate.missing’ function in the geomorph-package version 2.1.4 in R. This function interpolates the coordinates of a landmark of a reference specimen (obtained from a set of specimens for which all landmarks are present) on the specimen with missing landmarks using the thin-plate spline.
All wolf and domestic dog specimens were allocated to a dental age stage: if all permanent teeth were fully erupted into occlusion, specimens were described as adults. If deciduous and not jet fully erupted permanent teeth were still present, the specimens were described as juveniles. For the wolf and every group/breed of domestic dog, ontogenetic series were sampled. This was, however, not possible for the archaeological specimens. The youngest domestic dog (Akita) and wolf are comparable in absolute age (12 and 13 days old). On the other extreme, domestic dog and wolf specimens of more than 10 years of age have been sampled.
All analyses were conducted using Microsoft Excel 2010, R version 3.2.155 and RStudio version 1.0.13656. The mean of both replicates was calculated for every landmark in all specimens that have been digitized twice. The complete dataset is available as part of the supplementary data (Supplementary Table S2).
A generalized Procrustes superimposition was computed to extract geometric shape of the investigated specimens simultaneously. Subsequently, a covariance matrix of the symmetric component of averaged Procrustes shape coordinates (average of the left and right sides of the skull) was generated. Based on this covariance matrix, a principal component analysis (PCA) was performed to explore patterns of shape variation in domestic dogs and wolves. Differences between groups and age classes in skull shape were assessed with MANOVAs and quantified using Mahalanobis distances. P-values from MANOVAs were corrected for multiple testing.
Phenotypic ontogenetic trajectories of wolves and domestic dogs in shape space were compared57,58 using the ‘trajectory.analysis’ function in the geomorph-package version 2.1.459 in R. In doing so, cranial shape changes from juveniles to adults were quantified and compared between wolf and groups/breeds of domestic dogs indicated in Table 1. This analysis compares the trajectory size defined as the path-length distance along the trajectory, and the trajectory orientation (direction) defined as the direction of first principal component of the covariance matrix estimated from the trajectory points after standardisation for the starting point21. A permutation procedure (1000 random permutations) was used to statistically test differences of sampling distributions.
All data generated or analysed during this study are included in this published article (and its supplementary information files).
Darwin, C. The variation of animals and plants under domestication. (BiblioBazaar, 1868).
Herre, W. & Röhrs, M. Haustiere - zoologisch gesehen. (Gustav Fischer Verlag, 1990).
Clutton-Brock, J. A natural history of domesticated mammals. (Cambridge University Press, 1999).
Sánchez-Villagra, M. R., Geiger, M. & Schneider, R. A. The taming of the neural crest: a developmental perspective on the origins of morphological covariation in domesticated mammals. R. Soc. Open Sci. 3, 160107 (2016).
Dechambre, E. La theorie de la foetalisation et la formation des races de chiens et de porcs. Mammalia 13, 129–137 (1949).
Morey, D. F. Size, shape and development in the evolution of the domestic dog. J. Archaeol. Sci. 19, 181–204 (1992).
Wayne, R. K. Cranial morphology of domestic and wild canids: the influence of development on morphological change. Evolution (N.Y). 243–261 (1986).
Bolk, L. Das Problem der Menschwerdung. (Fischer, 1926).
Morey, D. F. The early evolution of the domestic dog. Am. Sci. 82, 336–347 (1994).
Coppinger, R. & Schneider, R. In The domestic dog: its evolution, behaviour, and interactions with people (ed. Serpell, J.) 21–47 (Cambridge University Press, 1995).
Price, E. O. Behavioral development in animals undergoing domestication. Appl. Anim. Behav. Sci. 65, 245–271 (1999).
Klatt, B. Über den Einfluss der Gesamtgrösse auf das Schädelbild nebst Bemerkungen über die Vorgeschichte der Haustiere. Arch. für Entwicklungsmechanik der Org. 36, 387–471 (1913).
Starck, D. Der heutige Stand des Fetalisationsproblems. Zeitschrift für Tierzüchtung und Züchtungsbiologie 77, 129–155 (1962).
Rosenberg, K. F. Die postnatale Proportionsänderung der Schädel zweier extremer Wuchsformen des Haushundes. Zeitschrift für Tierzüchtung und Züchtungsbiologie 82, 1–36 (1966).
Drake, A. G. Dispelling dog dogma: an investigation of heterochrony in dogs using 3D geometric morphometric analysis of skull shape. Evol. Dev. 13, 204–213 (2011).
Haeckel, E. H. P. A. Generelle Morphologie der Organismen: allgemeine Grundzüge der organischen Formen-Wissenschaft, mechanisch begründet durch die von Charles Darwin reformirte Descendenz-Theorie. (Reimer, 1866).
Zollikofer, C. P. E. & Ponce De León, M. S. Kinematics of cranial ontogeny: Heterotopy, heterochrony, and geometric morphometric analysis of growth models. J. Exp. Zool. Part B Mol. Dev. Evol. 302B, 322–340 (2004).
Zollikofer, C. P. E. & de León, M. S. P. The evolution of hominin ontogenies. Semin. Cell Dev. Biol. 21, 441–452 (2010).
Klingenberg, C. P. Evolution and development of shape: integrating quantitative approaches. Nat. Rev. Genet. 11, 623–635 (2010).
Lawing, A. M. & Polly, P. D. Geometric morphometrics: recent applications to the study of evolution and development. J. Zool. 280, 1–7 (2010).
Adams, D. C. & Collyer, M. L. A general framework for the analysis of phenotypic trajectories in evolutionary studies. Evolution (N.Y). 63, 1143–1154 (2009).
Bookstein, F. Morphometric tools for landmark data: geometry and biology (1997).
Mitteroecker, P., Gunz, P. & Bookstein, F. L. Heterochrony and geometric morphometrics: a comparison of cranial growth in Pan paniscus versus Pan troglodytes. Evol. Dev. 7, 244–258 (2005).
León, M. & Zollikofer, C. Neanderthals and modern humans—chimps and bonobos: similarities and differences in development and evolution. Neanderthals Revisit. New approaches (2006).
Alberch, P., Gould, S. J., Oster, G. F. & Wake, D. B. Size and shape in ontogeny and phylogeny. Paleobiology 296–317 (1979).
Tchernov, E. & Horwitz, L. K. Body size diminution under domestication: unconscious selection in primeval domesticates. J. Anthropol. Archaeol. 10, 54–75 (1991).
Lord, K., Feinstein, M., Smith, B. & Coppinger, R. Variation in reproductive traits of members of the genus Canis with special attention to the domestic dog (Canis familiaris). Behav. Processes 92, 131–142 (2013).
Trut, L., Oskina, I. & Kharlamova, A. Animal evolution during domestication: the domesticated fox as a model. Bioessays 31, 349–360 (2009).
Goerttler, V. Neufundländer. (Die neue Brehm-Bücherei, Franckh’sche Verlagshandlung Stuttgart, Kosmos-Verlag, 1966).
Drake, A. G. & Klingenberg, C. P. The pace of morphological change: historical transformation of skull shape in St Bernard dogs. Proc. R. Soc. London B Biol. Sci. 275, 71–76 (2008).
Krämer, E. M. Faszination Rassehunde–Herkunft & Aufgaben, Temperament & Wesen. (Franckh-Kosmos Verlag, 2013).
Hendricks, J. C. & Pack, A. Animal models of sleep apnea. In Sleep and Breathing (eds. Saunders, N. A. & Sullivan, C. E.) 847–866 (Marcel Dekker Inc., 1994).
Boyko, A. R. et al. A simple genetic architecture underlies morphological variation in dogs. PLoS Biol. 8, e1000451 (2010).
Larson, G. et al. Rethinking dog domestication by integrating genetics, archeology, and biogeography. Proc. Natl. Acad. Sci. 109, 8878–8883 (2012).
Parker, H. G. et al. Genetic structure of the purebred domestic dog. Science (80-.). 304, 1160–1164 (2004).
Vonholdt, B. M. et al. Genome-wide SNP and haplotype analyses reveal a rich history underlying dog domestication. Nature 464, 898–902 (2010).
Savolainen, P., Leitner, T., Wilton, A. N., Matisoo-Smith, E. & Lundeberg, J. A detailed picture of the origin of the Australian dingo, obtained from the study of mitochondrial DNA. Proc. Natl. Acad. Sci. USA. 101, 12387–12390 (2004).
Lord, K., Coppinger, L. & Coppinger, R. Differences in the behavior of landraces and breeds of dogs. In Genetics and the behavior of domestic animals (eds. Grandin, T. & Deesing, M. J.) 195–235 (Elsevier, 2014).
Studer, T. Die prähistorischen Hunde in ihrer Beziehung zu den gegenwärtig lebenden Rassen. Abhandlungen der Schweizerischen paläontologischen Gesellschaft 28 (1901).
Germonpré, M. et al. Fossil dogs and wolves from Palaeolithic sites in Belgium, the Ukraine and Russia: osteometry, ancient DNA and stable isotopes. J. Archaeol. Sci. 36, 473–490 (2009).
Sablin, M. & Khlopachev, G. The earliest Ice Age dogs: evidence from Eliseevichi. Curr. Anthropol. (2002).
Drake, A., Coquerelle, M. & Colombeau, G. 3D morphometric analysis of fossil canid skulls contradicts the suggested domestication of dogs during the late Paleolithic. Sci. Rep. (2015).
Ovodov, N. D. et al. A 33,000-year-old incipient dog from the Altai mountains of Siberia: evidence of the earliest domestication disrupted by the last glacial maximum. PLoS One 6 (2011).
Kirkwood, J. K. The influence of size on the biology of the dog. J. Small Anim. Pract. 26, 97–110 (1985).
Clauss, M., Dittmann, M. T., Mueller, D. W. H., Zerbe, P. & Codron, D. Low scaling of a life history variable: analysing eutherian gestation periods with and without phylogeny-informed statistics. Mamm. Biol. 79, 9–16 (2014).
Concannon, P. W. Canine pregnancy: predicting parturition and timing events of gestation. In Recent Advances in Small Animal Reproduction . International Veterinary Information Services (eds. Concannon, P. W., England, E. & J. V.) (International Veterinary Information Service, 2000).
Seal, U. S., Plotka, E. D., Packard, J. M. & Mech, L. D. Endocrine correlates of reproduction in the wolf. I. Serum progesterone, estradiol and LH during the estrous cycle. Biol. Reprod. 21, 1057–1066 (1979).
Werneburg, I. & Geiger, M. Ontogeny of domestic dogs and the developmental foundations of carnivoran domestication. J. Mamm. Evol. 1–21 (2016).
Hare, B. Survival of the friendliest: Homo sapiens evolved via selection for prosociality. Annu. Rev. Psychol. 68, 155–186 (2017).
Zeveloff, S. & Boyce, M. Why human neonates are so altricial. Am. Nat (1982).
Klingenberg, C. P., Barluenga, M. & Meyer, A. Shape analysis of symmetric structures: quantifying variation among individuals and asymmetry. Evolution (N.Y). 56, 1909–1920 (2002).
O’Regan, H. J. & Kitchener, A. C. The effects of captivity on the morphology of captive, domesticated and feral mammals. Mamm. Rev 35, 215–230 (2005).
Epstein, H. The origin of the domestic animals of Africa. 1, (Africana, 1971).
Gascho, D., Beutler, S., Mainini, C. & Geiger, M. 3D models of three wolf pup skulls related to the publication: Neomorphosis and heterochrony of skull shape in dog domestication. MorphoMuseuM 3(4), e6. https://doi.org/10.18563/m3.3.4.e6 (2017)
R Core Team. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria, 2012 (2012).
RStudio. RStudio: Integrated development environment for R (2012).
Collyer, M. L. & Adams, D. C. Phenotypic trajectory analysis: comparison of shape change patterns in evolution and ecology. Hystrix 24, 75–83 (2013).
Collyer, M. L. & Adams, D. C. Analysis of two-state multivariate phenotypic change in ecological studies. Ecology 88, 683–692 (2007).
Adams, D. C. & Otárola‐Castillo, E. geomorph: an R package for the collection and analysis of geometric morphometric shape data. Methods Ecol. Evol 4, 393–399 (2013).
The authors are grateful to Sabrina Beutler, Marianne Haffner (ZMUZH), Daniela Kalthoff (NRM), Renate Lücht (I.f.H.), and Marc Nussbaumer (NMBE) for providing access to specimens and to Campbell Rolian for helpful comments which have substantially improved the manuscript. This work has been supported by the Swiss National Science Foundation grant 31003A_149605 to MRSV.
The authors declare that they have no competing interests.
Publisher's note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Cite this article
Geiger, M., Evin, A., Sánchez-Villagra, M.R. et al. Neomorphosis and heterochrony of skull shape in dog domestication. Sci Rep 7, 13443 (2017). https://doi.org/10.1038/s41598-017-12582-2
Disruptive natural selection by male reproductive potential prevents underexpression of protein-coding genes on the human Y chromosome as a self-domestication syndrome
BMC Genetics (2020)
Old dogs, new tricks: 3D geometric analysis of cranial morphology supports ancient population substructure in the Australian dingo
Frontiers in Zoology (2019)
Frontiers in Zoology (2018)