Table 2 The number of fish taxa and OTUs (class Actinopterygii) identified at Coral Bay in west Australia across PCR assays used in the study.

From: Ecosystem biomonitoring with eDNA: metabarcoding across the tree of life in a tropical marine environment

Primer Set Order Family Genus Species OTUs
18S_V4F 4 2    16
18S_VR
18S_1 F 1     2
18S_400 R
m1COIintF 6 12 15 9 16
jgHCO2198
16Smam1 1 2 2 2 2
16Smam2
16SF/D 10 38 61 29 143
16S2R-degenerate
Multiple Hits 7 12 14 6  
  1. The row ‘Multiple Hits’ refers to the number of taxa identified with more than one PCR assay. OTUs were generated using a 98% similarity cut-off.